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JoVE Science Education

General Laboratory Techniques

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Basic Methods in Cellular and Molecular Biology

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Model Organisms I

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Essentials of
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Table of Contents

Table of Contents: Issue 75, May 2013

Jump to:  JoVE Editorial (1) JoVE Biology (18) JoVE Neuroscience (9) JoVE Immunology and Infection (8) JoVE Clinical and Translational Medicine (9) JoVE Bioengineering (5) JoVE Applied Physics (3) JoVE Chemistry (2)
 JoVE Biology

A Novel Method for the Culture and Polarized Stimulation of Human Intestinal Mucosa Explants

1Department of Experimental Oncology, European Institute of Oncology, 2Department of Pathology and Laboratory Medicine, European Institute of Oncology, 3U.O. Gastroenterologia 2, IRCCS Ca' Granda, Ospedale Policlinico di Milano


JoVE 4368

Date Published: May 1, 2013  |  doi:10.3791/4368  |  Section: JoVE Biology

 JoVE Biology

Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass

1Department of Pathology, University of Utah School of Medicine


JoVE 50064

Date Published: May 1, 2013  |  doi:10.3791/50064  |  Section: JoVE Biology

 JoVE Biology

Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy

1Department of Biochemistry and Molecular Medicine, University of California, Davis, 2NSF Center for Biophotonics Science & Technology, University of California, Davis, 3University of Tromsø, 4Department of Surgery (Division of Surgical Oncology), University of California, Davis, 5UC Davis Comprehensive Cancer Center, University of California, Davis, 6Department of Biological Chemistry, University of California, Davis


JoVE 50047

Date Published: May 3, 2013  |  doi:10.3791/50047  |  Section: JoVE Biology

 JoVE Biology

Naïve Adult Stem Cells Isolation from Primary Human Fibroblast Cultures

1Department of Dermatology and Institute for Medical Engineering, Technische Universität München, 2Department of Dermatology and Allergology, Technische Universität München


JoVE 50185

Date Published: May 3, 2013  |  doi:10.3791/50185  |  Section: JoVE Biology

 JoVE Biology

Whole-Body Nanoparticle Aerosol Inhalation Exposures

1Department of Physiology and Pharmacology, School of Medicine, West Virginia University, 2Center for Cardiovascular and Respiratory Sciences, West Virginia University, 3National Institute for Occupational Safety and Health


JoVE 50263

Date Published: May 7, 2013  |  doi:10.3791/50263  |  Section: JoVE Biology

 JoVE Biology

Direct Imaging of ER Calcium with Targeted-Esterase Induced Dye Loading (TED)

1Institute for Clinical Neurobiology, University of Wuerzburg, 2Department of Synapses - Circuits - Plasticity, Max Planck Institute of Neurobiology, Martinsried, 3Walter Brendel Centre of Experimental Medicine, Ludwig-Maximilians University of Munich


JoVE 50317

Date Published: May 7, 2013  |  doi:10.3791/50317  |  Section: JoVE Biology

 JoVE Biology

A High-throughput Method for Measurement of Glomerular Filtration Rate in Conscious Mice

1Division of Nephrology-Hypertension, Department of Medicine, University of California, San Diego, 2San Diego VA Healthcare System


JoVE 50330

Date Published: May 10, 2013  |  doi:10.3791/50330  |  Section: JoVE Biology

 JoVE Biology

Isolation of Native Soil Microorganisms with Potential for Breaking Down Biodegradable Plastic Mulch Films Used in Agriculture

1Biology Department, Western Washington University, 2Washington State University Northwestern Research and Extension Center, 3Department of Plant and Soil Science, Texas Tech University


JoVE 50373

Date Published: May 10, 2013  |  doi:10.3791/50373  |  Section: JoVE Biology

 JoVE Biology

Introduction to Solid Supported Membrane Based Electrophysiology

1Department of Biophysical Chemistry, Max Planck Institute of Biophysics, 2Buchmann Institute for Molecular Life Sciences, Goethe University Frankfurt


JoVE 50230

Date Published: May 11, 2013  |  doi:10.3791/50230  |  Section: JoVE Biology

 JoVE Biology

Protein Transfection of Mouse Lung

1Department of Medicine, St. Luke's Roosevelt Medical Center


JoVE 50080

Date Published: May 15, 2013  |  doi:10.3791/50080  |  Section: JoVE Biology

 JoVE Biology

Evaluation of Respiratory System Mechanics in Mice using the Forced Oscillation Technique

1Meakins-Christie Laboratories, Department of Medicine, McGill University, 2SCIREQ Scientific Respiratory Equipment Inc.


JoVE 50172

Date Published: May 15, 2013  |  doi:10.3791/50172  |  Section: JoVE Biology

 JoVE Biology

A Practical and Novel Method to Extract Genomic DNA from Blood Collection Kits for Plasma Protein Preservation

1Division of Gastroenterology, Hepatology and Nutrition, Department of Pediatrics, Emory University School of Medicine and Children's Health Care of Atlanta, 2Division of Rheumatology, Department of Pediatrics, Emory University School of Medicine and Children's Health Care of Atlanta


JoVE 4241

Date Published: May 18, 2013  |  doi:10.3791/4241  |  Section: JoVE Biology

 JoVE Biology

Live Cell Cycle Analysis of Drosophila Tissues using the Attune Acoustic Focusing Cytometer and Vybrant DyeCycle Violet DNA Stain

1Molecular, Cellular and Developmental Biology, University of Michigan


JoVE 50239

Date Published: May 19, 2013  |  doi:10.3791/50239  |  Section: JoVE Biology

 JoVE Biology

Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence

1Department of Medicine, Albert Einstein College of Medicine, 2Diabetes Research and Training Center, Albert Einstein College of Medicine, 3Department of Genetics, Albert Einstein College of Medicine


JoVE 50246

Date Published: May 22, 2013  |  doi:10.3791/50246  |  Section: JoVE Biology

 JoVE Biology

Measuring Intracellular Ca2+ Changes in Human Sperm using Four Techniques: Conventional Fluorometry, Stopped Flow Fluorometry, Flow Cytometry and Single Cell Imaging

1Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología-Universidad Nacional Autónoma de México, 2Math and Sciences Department, Edison State College


JoVE 50344

Date Published: May 24, 2013  |  doi:10.3791/50344  |  Section: JoVE Biology

 JoVE Biology

A Novel Ex vivo Culture Method for the Embryonic Mouse Heart

1McAllister Heart Institute, University of North Carolina at Chapel Hill


JoVE 50359

Date Published: May 24, 2013  |  doi:10.3791/50359  |  Section: JoVE Biology

 JoVE Biology

RNA Secondary Structure Prediction Using High-throughput SHAPE

1RT Biochemistry Section, HIV Drug Resistance Program, Frederick National Laboratory for Cancer Research


JoVE 50243

Date Published: May 31, 2013  |  doi:10.3791/50243  |  Section: JoVE Biology

 JoVE Biology

Application of an In vitro DNA Protection Assay to Visualize Stress Mediation Properties of the Dps Protein

1Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology


JoVE 50390

Date Published: May 31, 2013  |  doi:10.3791/50390  |  Section: JoVE Biology

 JoVE Neuroscience

F1FO ATPase Vesicle Preparation and Technique for Performing Patch Clamp Recordings of Submitochondrial Vesicle Membranes

1Department of Internal Medicine, Yale University


JoVE 4394

Date Published: May 4, 2013  |  doi:10.3791/4394  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Direct Intraventricular Delivery of Drugs to the Rodent Central Nervous System

1Department of Neurology, Washington University in St. Louis School of Medicine


JoVE 50326

Date Published: May 12, 2013  |  doi:10.3791/50326  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Implementing Dynamic Clamp with Synaptic and Artificial Conductances in Mouse Retinal Ganglion Cells

1Discipline of Biomedical Science, School of Medical Sciences, Sydney Medical School and Bosch Institute, University of Sydney, 2The MARCS Institute, University of Western Sydney, 3Discipline of Physiology, School of Medical Sciences, Sydney Medical School and Bosch Institute, University of Sydney


JoVE 50400

Date Published: May 16, 2013  |  doi:10.3791/50400  |  Section: JoVE Neuroscience

 JoVE Neuroscience

A Caenorhabditis elegans Model System for Amylopathy Study

1Department of Neuroscience and Cell Biology, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey


JoVE 50435

Date Published: May 17, 2013  |  doi:10.3791/50435  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Three Dimensional Vestibular Ocular Reflex Testing Using a Six Degrees of Freedom Motion Platform

1Department of Neuroscience, Erasmus MC, 2TNO Human Factors


JoVE 4144

Date Published: May 23, 2013  |  doi:10.3791/4144  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Micromanipulation of Gene Expression in the Adult Zebrafish Brain Using Cerebroventricular Microinjection of Morpholino Oligonucleotides

1DFG-Center for Regenerative Therapies Dresden, Cluster of Excellence (CRTD) and Biotechnology Center (BIOTEC) of the Technische Universität Dresden


JoVE 50415

Date Published: May 23, 2013  |  doi:10.3791/50415  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Designing and Implementing Nervous System Simulations on LEGO Robots

1Marine Science Center, Northeastern University, 2Fachrichtung Bionik, Bremen University of Applied Sciences


JoVE 50519

Date Published: May 25, 2013  |  doi:10.3791/50519  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Electroporation of the Hindbrain to Trace Axonal Trajectories and Synaptic Targets in the Chick Embryo

1Koret School of Veterinary Medicine, The Hebrew University of Jerusalem, 2Department of Medical Neurobiology, The Hebrew University of Jerusalem


JoVE 50136

Date Published: May 29, 2013  |  doi:10.3791/50136  |  Section: JoVE Neuroscience

 JoVE Immunology and Infection

Trans-vivo Delayed Type Hypersensitivity Assay for Antigen Specific Regulation

1Department of Surgery, University of Wisconsin-Madison, School of Medicine and Public Health


JoVE 4454

Date Published: May 2, 2013  |  doi:10.3791/4454  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Oral Transmission of Listeria monocytogenes in Mice via Ingestion of Contaminated Food

1Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky


JoVE 50381

Date Published: May 6, 2013  |  doi:10.3791/50381  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Accelerated Type 1 Diabetes Induction in Mice by Adoptive Transfer of Diabetogenic CD4+ T Cells

1Department of Microbiology & Immunology, Pennsylvania State University College of Medicine


JoVE 50389

Date Published: May 6, 2013  |  doi:10.3791/50389  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

A Simple Protocol for Platelet-mediated Clumping of Plasmodium falciparum-infected Erythrocytes in a Resource Poor Setting

1Malawi-Liverpool-Wellcome Trust Clinical Research Programme, 2Liverpool School of Tropical Medicine, 3Department of Microbiology, Division of Medical Parasitology, New York University School of Medicine


JoVE 4316

Date Published: May 16, 2013  |  doi:10.3791/4316  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Isolation of Adipose Tissue Immune Cells

1Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine


JoVE 50707

Date Published: May 22, 2013  |  doi:10.3791/50707  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'

1Department of Microbial and Environmental Genomics, The J. Craig Venter Institute


JoVE 3899

Date Published: May 26, 2013  |  doi:10.3791/3899  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

New Tools to Expand Regulatory T Cells from HIV-1-infected Individuals

1Ragon Institute of MGH, MIT, and Harvard, 2Division of Infectious Diseases, Massachusetts General Hospital


JoVE 50244

Date Published: May 30, 2013  |  doi:10.3791/50244  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Determination of Tolerable Fatty Acids and Cholera Toxin Concentrations Using Human Intestinal Epithelial Cells and BALB/c Mouse Macrophages

1Department of Biological Sciences, Kingsborough Community College, 2Institute for Cellular and Molecular Biology, University of Texas at Austin, 3New Jersey Center for Science, Technology and Mathematics, Kean University


JoVE 50491

Date Published: May 30, 2013  |  doi:10.3791/50491  |  Section: JoVE Immunology and Infection

 JoVE Clinical and Translational Medicine

Bilateral Common Carotid Artery Occlusion as an Adequate Preconditioning Stimulus to Induce Early Ischemic Tolerance to Focal Cerebral Ischemia

1Department of Neurology, Center for Stroke Research Berlin, Charité - Universitätsmedizin Berlin, Germany


JoVE 4387

Date Published: May 9, 2013  |  doi:10.3791/4387  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Controlled Cervical Laceration Injury in Mice

1Norton Neuroscience Institute, Norton Healthcare, 2Spinal Cord and Brain Injury Research Group, Stark Neurosciences Research Institute, Department of Neurological Surgery and Goodman and Campbell Brain and Spine, Medical Neuroscience Graduate Program, and Department of Anatomy and Cell Biology, Indiana University School of Medicine


JoVE 50030

Date Published: May 9, 2013  |  doi:10.3791/50030  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

A Murine Model of Stent Implantation in the Carotid Artery for the Study of Restenosis

1Institute for Molecular Cardiovascular Research, RWTH Aachen University, 2Institute for Textile Technology and Mechanical Engineering, RWTH Aachen University, 3Institute for Applied Medical Engineering, Helmholtz-Institute of RWTH Aachen University, 4Department of Experimental Molecular Imaging, RWTH Aachen University, 5Department of Oral and Maxillofacila Surgery, RWTH Aachen University


JoVE 50233

Date Published: May 14, 2013  |  doi:10.3791/50233  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Mouse Models for Graft Arteriosclerosis

1Department of Surgery, Yale University School of Medicine, 2Department of Pathology, Yale University School of Medicine


JoVE 50290

Date Published: May 14, 2013  |  doi:10.3791/50290  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

In vitro Organoid Culture of Primary Mouse Colon Tumors

1Department of Molecular & Integrative Physiology, University of Michigan, 2Department of Internal Medicine, Division of Gastroenterology, University of Michigan


JoVE 50210

Date Published: May 17, 2013  |  doi:10.3791/50210  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Matrix-assisted Autologous Chondrocyte Transplantation for Remodeling and Repair of Chondral Defects in a Rabbit Model

1Department of Orthopaedic Sports Medicine, Klinikum rechts der Isar der Technischen Universität München, 2Department of Radiology, Klinikum rechts der Isar der Technischen Universität München, 3Institute of Experimental Oncology and Therapy Research, Klinikum rechts der Isar der Technischen Universität München, 4Department of Radiology, Uniklinik Köln


JoVE 4422

Date Published: May 21, 2013  |  doi:10.3791/4422  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Treatment of Osteochondral Defects in the Rabbit's Knee Joint by Implantation of Allogeneic Mesenchymal Stem Cells in Fibrin Clots

1Department of Orthopaedic Sports Medicine, Klinikum rechts der Isar der Technischen Universität München, 2Department of Radiology, Klinikum rechts der Isar der Technischen Universität München, 3Institute of Experimental Oncology and Therapy Research, Klinikum rechts der Isar der Technischen Universität München, 4Department of Radiology, Uniklinik Köln


JoVE 4423

Date Published: May 21, 2013  |  doi:10.3791/4423  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Minimal Erythema Dose (MED) Testing

1Cancer Prevention and Control Program, Fox Chase Cancer Center, 2Department of Psychiatry, University of Pennsylvania, 3Department of Psychology, Drexel University, 4Department of Medicine, Fox Chase Cancer Center, 5Cancer Prevention and Control Program, The Cancer Institute of New Jersey


JoVE 50175

Date Published: May 28, 2013  |  doi:10.3791/50175  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Murine Model of Wound Healing

1The Heart Research Institute, 2Sydney Medical School, University of Sydney, 3Cardiology Department, Royal Prince Alfred Hospital


JoVE 50265

Date Published: May 28, 2013  |  doi:10.3791/50265  |  Section: JoVE Clinical and Translational Medicine

 JoVE Bioengineering

Microfluidic-based Electrotaxis for On-demand Quantitative Analysis of Caenorhabditis elegans' Locomotion

1Department of Biology, McMaster University, 2Department of Mechanical Engineering, McMaster University


JoVE 50226

Date Published: May 2, 2013  |  doi:10.3791/50226  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Manufacturing Devices and Instruments for Easier Rat Liver Transplantation

1Transplantation Division, Department of Surgery, University of Geneva Hospitals, 2Department of Surgery, University of Pavia, 3Department of Surgery, University of Geneva, 4Division of Abdominal Surgery, Department of Surgery, University of Geneva Hospitals


JoVE 50380

Date Published: May 5, 2013  |  doi:10.3791/50380  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

1Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn University, 2Clinical Research Laboratory, 81st Medical Group, Keesler Air Force Base


JoVE 50474

Date Published: May 8, 2013  |  doi:10.3791/50474  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Air Filter Devices Including Nonwoven Meshes of Electrospun Recombinant Spider Silk Proteins

1Biomaterials Research Group, University of Bayreuth


JoVE 50492

Date Published: May 8, 2013  |  doi:10.3791/50492  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Measurement of Tension Release During Laser Induced Axon Lesion to Evaluate Axonal Adhesion to the Substrate at Piconewton and Millisecond Resolution

1Institute of Biophysics, National Research Council of Italy, 2Dipartimento di Sistemi e Informatica, Università di Firenze, 3Department of Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia


JoVE 50477

Date Published: May 27, 2013  |  doi:10.3791/50477  |  Section: JoVE Bioengineering

 JoVE Applied Physics

Synthesis and Functionalization of Nitrogen-doped Carbon Nanotube Cups with Gold Nanoparticles as Cork Stoppers

1Department of Chemistry, University of Pittsburgh


JoVE 50383

Date Published: May 13, 2013  |  doi:10.3791/50383  |  Section: JoVE Applied Physics

 JoVE Applied Physics

Measuring Spatially- and Directionally-varying Light Scattering from Biological Material

1Department of Biomedical Science, Cornell University, 2Department of Ecology and Evolutionary Biology, Cornell University, 3Cornell University Museum of Vertebrates, 4Department of Computer Science, Cornell University


JoVE 50254

Date Published: May 20, 2013  |  doi:10.3791/50254  |  Section: JoVE Applied Physics

 JoVE Applied Physics

In-situ Tapering of Chalcogenide Fiber for Mid-infrared Supercontinuum Generation

1Edward L. Ginzton Laboratory, Stanford University


JoVE 50518

Date Published: May 27, 2013  |  doi:10.3791/50518  |  Section: JoVE Applied Physics

 JoVE Chemistry

Fabricating Nanogaps by Nanoskiving

1Stratingh Institute for Chemistry and Zernike Institute for Advanced Materials, University of Groningen


JoVE 50406

Date Published: May 13, 2013  |  doi:10.3791/50406  |  Section: JoVE Chemistry

 JoVE Chemistry

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)

1Centers for Cancer Pharmacology and Excellence in Environmental Toxicology, Department of Pharmacology, University of Pennsylvania


JoVE 50433

Date Published: May 20, 2013  |  doi:10.3791/50433  |  Section: JoVE Chemistry

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