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The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.

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Table of Contents

Table of Contents: Issue 59, January 2012

Jump to:  JoVE Editorial (1) JoVE General (18) JoVE Neuroscience (12) JoVE Immunology and Infection (5) JoVE Clinical and Translational Medicine (8) JoVE Bioengineering (9)
 JoVE General

Modified Yeast-Two-Hybrid System to Identify Proteins Interacting with the Growth Factor Progranulin


JoVE 3562

1, 1, 1,2

1Department of Orthopaedic Surgery, NYU Hospital for Joint Diseases, 2Department of Cell Biology, New York University School of Medicine

Date Published: January 17, 2012  |  doi:10.3791/3562  |  Section: JoVE General

Keywords: Molecular Biology, Modified yeast two-hybrid screen, PGRN, TNFR2, inflammation, autoimmune diseases

 JoVE General

Analysis of Cell Cycle Position in Mammalian Cells


JoVE 3491

1, 1, 2

1Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, 2London Regional Cancer Program, Children's Health Research Institute, and Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario

Date Published: January 21, 2012  |  doi:10.3791/3491  |  Section: JoVE General

Keywords: Molecular Biology, cell cycle, proliferation, flow cytometry, DNA synthesis, fluorescence

 JoVE General

Quantitative Live Cell Fluorescence-microscopy Analysis of Fission Yeast


JoVE 3454

1, 1,2, 1

1Science for Life Laboratory, Department of Medical Biochemistry and Microbiology, University of Uppsala, 2Department of Microbiology, Swedish University of Agricultural Sciences

Date Published: January 23, 2012  |  doi:10.3791/3454  |  Section: JoVE General

Keywords: Molecular Biology, Fission yeast, fluorescence microscopy, nuclear organisation, chromatin, GFP

 JoVE General

Quantifying Mixing using Magnetic Resonance Imaging


JoVE 3493

1, 1, 2, 2, 1

1Dept. Food Science and Technology, University of California, Davis, 2Corporate Engineering and Technology Laboratory, Procter & Gamble Company

Date Published: January 25, 2012  |  doi:10.3791/3493  |  Section: JoVE General

Keywords: Biophysics, Magnetic resonance imaging, MRI, mixing, rheology, static mixer, split-and-recombine mix

 JoVE General

Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids


JoVE 3583

1, 2, 1, 1, 3, 1

1Department of Energy, Environmental and Chemical Engineering, Washington University, 2Department of Biology, Washington University, 3Department of Energy, Environmental and Chemical Engineering and Department of Biology, Washington University

Date Published: January 26, 2012  |  doi:10.3791/3583  |  Section: JoVE General

Keywords: Molecular Biology, GC-MS, novel pathway, metabolism, labeling, phototrophic microorganism

 JoVE Neuroscience

Cut-loading: A Useful Tool for Examining the Extent of Gap Junction Tracer Coupling Between Retinal Neurons


JoVE 3180

1, 2, 1

1Department of Neuroscience, Ohio State University College of Medicine, 2Department of Ophthalmology and Visual Science, University of Texas Medical School

Date Published: January 12, 2012  |  doi:10.3791/3180  |  Section: JoVE Neuroscience

Keywords: Neuroscience, retina, photoreceptors, gap junctions, tracer coupling, neurobiotin, labeling

 JoVE Neuroscience

Monitoring Cleaved Caspase-3 Activity and Apoptosis of Immortalized Oligodendroglial Cells using Live-cell Imaging and Cleaveable Fluorogenic-dye Substrates Following Potassium-induced Membrane Depolarization


JoVE 3422

, ,

Department of Molecular and Cellular Biology, University of Guelph

Date Published: January 13, 2012  |  doi:10.3791/3422  |  Section: JoVE Neuroscience

Keywords: Neuroscience, myelin basic protein, apoptosis, neuroprotection, caspase-3, live-cell imaging, glia, oligodendrocytes

 JoVE Neuroscience

Analysis of Trunk Neural Crest Cell Migration using a Modified Zigmond Chamber Assay


JoVE 3330

1, 2, 1

1Department of Biology, California State University, Northridge, 2Centro de Biología Celular y Molecular, Universidad Nacional de Córdoba

Date Published: January 19, 2012  |  doi:10.3791/3330  |  Section: JoVE Neuroscience

Keywords: Neuroscience, neural crest, cell migration, primary culture, chemotaxis, chemokinesis, Zigmond, cell polarity, explant culture, microdissection

 JoVE Neuroscience

Patch-clamp Capacitance Measurements and Ca2+ Imaging at Single Nerve Terminals in Retinal Slices


JoVE 3345

, ,

The Vollum Institute, Oregon Health and Science University

Date Published: January 19, 2012  |  doi:10.3791/3345  |  Section: JoVE Neuroscience

Keywords: Neuroscience, synaptic physiology, axon terminal, synaptic ribbon, retina microcircuits, goldfish, zebrafish, brain slices, patch-clamp, membrane capacitance measurements, calcium-imaging, exocytosis, transmitter release

 JoVE Neuroscience

Tissue Preparation and Immunostaining of Mouse Sensory Nerve Fibers Innervating Skin and Limb Bones


JoVE 3485

1, 1,2

1Department of Pharmacology, The University of Iowa, 2Department of Anesthesia, Roy J. and Lucille A. Carver College of Medicine, The University of Iowa

Date Published: January 26, 2012  |  doi:10.3791/3485  |  Section: JoVE Neuroscience

Keywords: Neuroscience, pain, immunostaining, sensory nerve fiber, skin, bone, plantar punch, CGRP, NF200, TRPV1, Tubulin

 JoVE Neuroscience

The Tail Suspension Test


JoVE 3769

*1, *1,2, 3, 1, 1, 1,3,4

1Department of Psychiatry, University of Maryland School of Medicine, 2Tulane University School of Medicine, 3The Program in Neuroscience, University of Maryland, 4Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine

Date Published: January 28, 2012  |  doi:10.3791/3769  |  Section: JoVE Neuroscience

Keywords: Neuroscience, animal models, behavioral analysis, neuroscience, neurobiology, mood disorder, depression, mood stabilizer, antidepressant

 JoVE Neuroscience

The Mouse Forced Swim Test


JoVE 3638

1, 2, 1, 3, 1, 1,3,4

1Department of Psychiatry, University of Maryland School of Medicine, 2Tulane University School of Medicine, 3Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine, 4The Program in Neuroscience, University of Maryland

Date Published: January 29, 2012  |  doi:10.3791/3638  |  Section: JoVE Neuroscience

Keywords: Neuroscience, animal models, behavioral analysis, neuroscience, neurobiology, mood disorder, depression, mood stabilizer, antidepressant, forced swimming test, FST

 JoVE Neuroscience

Rodent Stereotaxic Surgery and Animal Welfare Outcome Improvements for Behavioral Neuroscience


JoVE 3528

1, 1, 1, 1, 1, 1, 1, 2, 1

1Department of Neuroscience, Section Anatomy, University Medical Center Groningen, University of Groningen, 2Animal Welfare Office, University of Groningen

Date Published: January 30, 2012  |  doi:10.3791/3528  |  Section: JoVE Neuroscience

Keywords: Neuroscience, stereotaxic surgery, cannula implantation, rat, refinement

 JoVE Immunology and Infection

Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance


JoVE 3686

, , ,

Department of Molecular Biology and Microbiology, University of Central Florida

Date Published: January 3, 2012  |  doi:10.3791/3686  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Surface plasmon resonance, AB toxin, translocation, endoplasmic reticulum, cell culture, cholera toxin, pertussis toxin

 JoVE Immunology and Infection

Locked Nucleic Acid Flow Cytometry-fluorescence in situ Hybridization (LNA flow-FISH): a Method for Bacterial Small RNA Detection


JoVE 3655

,

Center for Bio/Molecular Science and Engineering, Naval Research Laboratory

Date Published: January 10, 2012  |  doi:10.3791/3655  |  Section: JoVE Immunology and Infection

Keywords: Immunology, fluorescence in situ hybridization, FISH, flow cytometry, locked nucleic acid, sRNA, Vibrio

 JoVE Immunology and Infection

Intravital Microscopy of the Spleen: Quantitative Analysis of Parasite Mobility and Blood Flow


JoVE 3609

*1, *1, 2, 1,3

1Department of poverty related diseases, Barcelona Centre for International Health Research, 2Confocal Microscopy Unit, University of Barcelona- Scientific and Technological Centers, 3Institució Catalana de Recerca i Estudis Avançats (ICREA)

Date Published: January 14, 2012  |  doi:10.3791/3609  |  Section: JoVE Immunology and Infection

Keywords: Immunology, intravital microscopy, GFP, malaria, spleen, mobility, adhesion, Plasmodium yoelii, Balb/c mice

 JoVE Clinical and Translational Medicine

How to Measure Cortical Folding from MR Images: a Step-by-Step Tutorial to Compute Local Gyrification Index


JoVE 3417

1, 2,3, 4, 4, 2, 1

1Department of Psychiatry, University of Geneva School of Medicine, 2Signal Processing Laboratory, École Polytechnique Fédérale de Lausanne, 3Department of Radiology, University Hospital Center and University of Lausanne, 4Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital

Date Published: January 2, 2012  |  doi:10.3791/3417  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, neuroimaging, brain, cortical complexity, cortical development

 JoVE Clinical and Translational Medicine

Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue


JoVE 3396

1, 2, 1

1Obstetrics, Gynecology and Women’s Health and Division of Biological Sciences, University of Missouri, 2Obstetrics, Gynecology and Women’s Health and Animal Sciences, University of Missouri

Date Published: January 6, 2012  |  doi:10.3791/3396  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, mouse, rat, endometriosis, surgery, uterus, ectopic, endometriotic lesion

 JoVE Clinical and Translational Medicine

High Content Screening in Neurodegenerative Diseases


JoVE 3452

1, 2, 1

1Department of Clinical Genetics, VU University Medical Center, 2Center for Neurogenomics and Cognitive Research, Neuroscience Campus Amsterdam

Date Published: January 6, 2012  |  doi:10.3791/3452  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, High-throughput screening, high-content screening, neurodegeneration, automated cell culturing, Parkinson’s disease

 JoVE Clinical and Translational Medicine

Implantation of a Carotid Cuff for Triggering Shear-stress Induced Atherosclerosis in Mice


JoVE 3308

1, 2,3, 1, 3, 2, 4, 4, 1, 1

1European Institute for Molecular Imaging, Westfälische Wilhelms-University Münster, 2British Heart Foundation Cardiovascular Sciences Unit, Imperial College London, 3Department of Bioengineering, Imperial College London, 4Biomedical Engineering, Eindhoven University of Technology

Date Published: January 13, 2012  |  doi:10.3791/3308  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, atherosclerosis, mouse, cardiovascular disease, shear stress

 JoVE Clinical and Translational Medicine

Utilizing Transcranial Magnetic Stimulation to Study the Human Neuromuscular System


JoVE 3387

, ,

Ohio Musculoskeletal and Neurological Institute (OMNI) and the Department of Biomedical Sciences, Ohio University

Date Published: January 20, 2012  |  doi:10.3791/3387  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, neuroscience, muscle, electromyography, physiology, TMS, strength, motor control. sarcopenia, dynapenia, lumbar

 JoVE Clinical and Translational Medicine

Real-time fMRI Biofeedback Targeting the Orbitofrontal Cortex for Contamination Anxiety


JoVE 3535

1, 1, 2, 1, 1, 1, 2,3,4, 1, 1

1Department of Diagnostic Radiology, Yale University School of Medicine, 2Department of Psychiatry, Yale University School of Medicine, 3Yale Child Study Center, Yale University School of Medicine, 4Interdepartmental Neuroscience Program, Yale University School of Medicine

Date Published: January 20, 2012  |  doi:10.3791/3535  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Real-time fMRI, rt-fMRI, neurofeedback, biofeedback, orbitofrontal cortex, OFC, obsessive-compulsive disorder, OCD, contamination anxiety, resting connectivity

 JoVE Clinical and Translational Medicine

Femoral Arterial and Venous Catheterization for Blood Sampling, Drug Administration and Conscious Blood Pressure and Heart Rate Measurements


JoVE 3496

, ,

Department of Pharmacology and Toxicology, Michigan State University

Date Published: January 24, 2012  |  doi:10.3791/3496  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Rat, catheter, blood pressure, vein, artery, blood sampling, surgery, femoral

 JoVE Bioengineering

A Cre-Lox P Recombination Approach for the Detection of Cell Fusion In Vivo


JoVE 3581

1, 1, 1, 2

1Department of Biomedical Engineering, University of Wisconsin-Madison, 2Department of Biomedical Engineering, Materials Science Program, Laboratory for Optical and Computational Instrumentation, University of Wisconsin-Madison

Date Published: January 4, 2012  |  doi:10.3791/3581  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Cell fusion, stem cell, fusogen, cre recombinase, biophotonic imaging, cellular transplantation

 JoVE Bioengineering

Therapeutic Gene Delivery and Transfection in Human Pancreatic Cancer Cells using Epidermal Growth Factor Receptor-targeted Gelatin Nanoparticles


JoVE 3612

,

Department of Pharmaceutical Sciences, School of Pharmacy, Northeastern University

Date Published: January 4, 2012  |  doi:10.3791/3612  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Gelatin Nanoparticle, Gene Therapy, Targeted Delivery, Pancreatic Cancer, Epidermal Growth Factor Receptor, EGFR

 JoVE Bioengineering

In vitro Assembly of Semi-artificial Molecular Machine and its Use for Detection of DNA Damage


JoVE 3628

1,2, 1,2,3

1Neurosurgery, Baylor College of Medicine, 2Michael E. DeBakey Veterans Affairs Medical Center, 3Molecular & Cellular Biology, Baylor College of Medicine

Date Published: January 11, 2012  |  doi:10.3791/3628  |  Section: JoVE Bioengineering

Keywords: Bioengineering, molecular machine, bio-nanotechnology, 5'OH DNA breaks, 5'PO4 DNA breaks, apoptosis labeling, in situ detection, vaccinia topoisomerase I, DNA breaks, green nanotechnology

 JoVE Bioengineering

Gold Nanostar Synthesis with a Silver Seed Mediated Growth Method


JoVE 3570

1, 2, 1, 3

1Department of Physics and Astronomy, The University of Texas at San Antonio, 2Centro de Investigaciones en Optica A. C., 3Department of Biology and Neurosciences Institute, The University of Texas at San Antonio

Date Published: January 15, 2012  |  doi:10.3791/3570  |  Section: JoVE Bioengineering

Keywords: Bioengineering, thermal ablation, surface plasmon resonance, nanoparticle, nanotechnology, silver seeds

 JoVE Bioengineering

Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shear Stress


JoVE 3349

1, 2, 2, 3, 1, 1, 2, 2, 1, 4, 2, 1

1Department of Surgery, Duke University Medical Center, 2Department of Biomedical Engineering, Duke University, 3School of Medicine, University of Pennsylvania, 4Department of Medicine, Division of Cardiology, Duke University Medical Center

Date Published: January 17, 2012  |  doi:10.3791/3349  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Fluid Shear Stress, Shear Stress, Shear Force, Endothelium, Endothelial Progenitor Cells, Flow Chamber, Laminar Flow, Flow Circuit, Continuous Flow, Cell Adhesion

 JoVE Bioengineering

Rotating Cell Culture Systems for Human Cell Culture: Human Trophoblast Cells as a Model


JoVE 3367

*1, *1,2, 3, 1, 1

1Department of Microbiology and Immunology, Tulane University Medical School, 2Physician/Scientist Program, Tulane University Medical School, 3Department of Molecular and Cellular Biology, Baylor College of Medicine

Date Published: January 18, 2012  |  doi:10.3791/3367  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Extravillous trophoblasts, cytotrophoblast, invasion, matrix metalloproteinase, 3-D cell culture, RCCS, ECM, microcarriers

 JoVE Bioengineering

Polymer Microarrays for High Throughput Discovery of Biomaterials


JoVE 3636

1, 2, 1, 1, 3, 3, 2, 2, 1, 1

1Laboratory of Biophysics and Surface Analysis, University of Nottingham, 2School of Molecular Medical Sciences, University of Nottingham, 3David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology

Date Published: January 25, 2012  |  doi:10.3791/3636  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Materials discovery, Surface characterization, Polymer library, High throughput, Cell attachment

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