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General Laboratory Techniques

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Basic Methods in Cellular and Molecular Biology

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Model Organisms I

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Essentials of
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Table of Contents

Table of Contents: Issue 61, March 2012

Jump to:  JoVE Editorial (1) JoVE Biology (19) JoVE Neuroscience (10) JoVE Immunology and Infection (10) JoVE Clinical and Translational Medicine (7) JoVE Bioengineering (7) JoVE Errata (2)
 JoVE Biology

Avidity-based Extracellular Interaction Screening (AVEXIS) for the Scalable Detection of Low-affinity Extracellular Receptor-Ligand Interactions

1Cell Surface Signalling Laboratory, Wellcome Trust Sanger Institute


JoVE 3881

Date Published: March 5, 2012  |  doi:10.3791/3881  |  Section: JoVE Biology

 JoVE Biology

Expression and Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein in Saccharomyces cerevisiae

1Faculty of Life Sciences, University of Manchester


JoVE 3860

Date Published: March 10, 2012  |  doi:10.3791/3860  |  Section: JoVE Biology

 JoVE Biology

The ITS2 Database

1Department of Bioinformatics, Biocenter, University of Würzburg, 2Institute of Pharmacy and Food Chemistry, University of Würzburg


JoVE 3806

Date Published: March 12, 2012  |  doi:10.3791/3806  |  Section: JoVE Biology

 JoVE Biology

Determining the Contribution of the Energy Systems During Exercise

1Laboratory of Applied Nutrition, School of Physical Education and Sport, University of Sao Paulo, 2Aerobic Performance Research Group, School of Physical Education and Sport, University of Sao Paulo, 3Laboratory of Neuromuscular Adaptations to Strength Training, School of Physical Education and Sport, University of Sao Paulo, 4Martial Arts and Combat Sports Research Group, School of Physical Education and Sport, University of Sao Paulo


JoVE 3413

Date Published: March 20, 2012  |  doi:10.3791/3413  |  Section: JoVE Biology

 JoVE Biology

Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry

1Plant Molecular and Cellular Biology Program, University of Florida, 2Department of Biology, University of Florida, 3Interdisciplinary Center for Biotechnology Research, University of Florida, 4Genetics Institute, University of Florida


JoVE 3766

Date Published: March 24, 2012  |  doi:10.3791/3766  |  Section: JoVE Biology

 JoVE Biology

Endothelial Cell Co-culture Mediates Maturation of Human Embryonic Stem Cell to Pancreatic Insulin Producing Cells in a Directed Differentiation Approach

1Department of Bioengineering, University of Pittsburgh, 2Department of Chemical Engineering, University of Pittsburgh


JoVE 3759

Date Published: March 27, 2012  |  doi:10.3791/3759  |  Section: JoVE Biology

 JoVE Biology

In vivo Electroporation of Morpholinos into the Regenerating Adult Zebrafish Tail Fin

1Department of Biological Sciences, Center for Zebrafish Research, University of Notre Dame, 2Department of Microbiology, Immunology, and Pathology, Colorado State University, 3Departments of Anatomy and Cell Biology and Ophthalmology, Wayne State University School of Medicine


JoVE 3632

Date Published: March 29, 2012  |  doi:10.3791/3632  |  Section: JoVE Biology

 JoVE Biology

Sexual Development and Ascospore Discharge in Fusarium graminearum

1Genetics Program, Michigan State University, 2Department of Plant Biology, Michigan State University, 3Human Biology Program, Michigan State University, 4Department of Plant Pathology, Michigan State University


JoVE 3895

Date Published: March 29, 2012  |  doi:10.3791/3895  |  Section: JoVE Biology

 JoVE Biology

Isolation and Biophysical Study of Fruit Cuticles

1Department of Chemistry, City College of New York, City University of New York Graduate Center and Institute for Macromolecular Assemblies, 2Department of Chemical Engineering, City College of New York


JoVE 3529

Date Published: March 30, 2012  |  doi:10.3791/3529  |  Section: JoVE Biology

 JoVE Biology

Fruit Volatile Analysis Using an Electronic Nose

1Department of Plant Sciences, University of California, Davis, 2Department of Chemical Engineering and Material Science, University of California, Davis, 3Department of Viticulture and Enology, University of California, Davis


JoVE 3821

Date Published: March 30, 2012  |  doi:10.3791/3821  |  Section: JoVE Biology

 JoVE Biology

Affinity Precipitation of Active Rho-GEFs Using a GST-tagged Mutant Rho Protein (GST-RhoA(G17A)) from Epithelial Cell Lysates

1Keenan Research Centre, Li Ka Shing Knowledge Institute, St. Michael's Hospital, 2Department of Surgery, University of Toronto, 3Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill


JoVE 3932

Date Published: March 31, 2012  |  doi:10.3791/3932  |  Section: JoVE Biology

 JoVE Neuroscience

Large-scale Recording of Neurons by Movable Silicon Probes in Behaving Rodents

1Center for Molecular and Behavioral Neuroscience, University of New Jersey, 2Center for Interdisciplinary Research in Biology, Collège de France, 3Janelia Farm Research Campus, Howards Hughes Medical Institute, 4Deptartment of Psychology, University of Wisconsin at Milwaukee


JoVE 3568

Date Published: March 4, 2012  |  doi:10.3791/3568  |  Section: JoVE Neuroscience

 JoVE Neuroscience

A Polished and Reinforced Thinned-skull Window for Long-term Imaging of the Mouse Brain

1Department of Physics, University of California, San Diego, 2Department of Engineering Science and Mechanics, Pennsylvania State University, 3Department of Neurosurgery, Pennsylvania State University, 4Section of Neurobiology, University of California, San Diego


JoVE 3742

Date Published: March 7, 2012  |  doi:10.3791/3742  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Methods to Assay Drosophila Behavior

1Department of Pharmacology and Experimental Therapeutics, Louisiana State University Health Sciences Center, 2Department of Genetics, Louisiana State University Health Sciences Center


JoVE 3795

Date Published: March 7, 2012  |  doi:10.3791/3795  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Dissection and Culture of Mouse Dopaminergic and Striatal Explants in Three-Dimensional Collagen Matrix Assays

1Department of Neuroscience & Pharmacology, Rudolf Magnus Institute for Neuroscience, University Medical Center Utrecht


JoVE 3691

Date Published: March 23, 2012  |  doi:10.3791/3691  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Dissection of Adult Mouse Utricle and Adenovirus-mediated Supporting-cell Infection

1Department of Pathology and Laboratory Medicine, Medical University of South Carolina, 2Department of Microbiology & Immunology, Medical University of South Carolina, 3National Institute on Deafness and Other Communication Disorders, National Institutes of Health


JoVE 3734

Date Published: March 28, 2012  |  doi:10.3791/3734  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Preparation of Parasagittal Slices for the Investigation of Dorsal-ventral Organization of the Rodent Medial Entorhinal Cortex

1Neuroinformatics DTC, University of Edinburgh, 2Centre for Integrative Physiology, University of Edinburgh


JoVE 3802

Date Published: March 28, 2012  |  doi:10.3791/3802  |  Section: JoVE Neuroscience

 JoVE Immunology and Infection

Simple and Robust in vivo and in vitro Approach for Studying Virus Assembly

1Department of Plant Pathology and Microbiology, University of California, Riverside, 2Department of Bioengineering, University of California, Riverside


JoVE 3645

Date Published: March 1, 2012  |  doi:10.3791/3645  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Glass Wool Filters for Concentrating Waterborne Viruses and Agricultural Zoonotic Pathogens

1Wisconsin Water Science Center, United States Geological Survey, 2University of Wisconsin – Madison, 3Agricultural Research Service, United States Department of Agriculture, 4Alaska Science Center, United States Geological Survey


JoVE 3930

Date Published: March 3, 2012  |  doi:10.3791/3930  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

1Department of Microbiology and Parasitology, College of Basic Medical Sciences, China Medical University, 2Department of Obstetrics and Gynecology, College of Medicine, University of Arkansas for Medical Sciences, 3Department of Pathology, College of Medicine, University of Arkansas for Medical Sciences


JoVE 3657

Date Published: March 8, 2012  |  doi:10.3791/3657  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Imaging of HIV-1 Envelope-induced Virological Synapse and Signaling on Synthetic Lipid Bilayers

1Department of Pathology, New York University Langone School of Medicine, 2Program in Molecular Pathogenesis, Marty and Helen Kimmel Center for Biology and Medicine and Skirball Institute for Biomolecular Medicine, 3Laboratory of Molecular Immunogenetics, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, 4Veteran Affairs New York Harbor Healthcare System


JoVE 3757

Date Published: March 8, 2012  |  doi:10.3791/3757  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Infection of Zebrafish Embryos with Intracellular Bacterial Pathogens

1Department of Molecular Cell Biology, Institute of Biology, Leiden University, 2Department of Medical Microbiology and Infection Control, VU University Medical Center, 3Australian Regenerative Medicine Institute, Monash University


JoVE 3781

Date Published: March 15, 2012  |  doi:10.3791/3781  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Customization of Aspergillus niger Morphology Through Addition of Talc Micro Particles

1Institute of Biochemical Engineering, Technische Universität Braunschweig


JoVE 4023

Date Published: March 15, 2012  |  doi:10.3791/4023  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Detection of Invasive Pulmonary Aspergillosis in Haematological Malignancy Patients by using Lateral-flow Technology

1Biosciences, University of Exeter, 2BICMS, Queen Mary University of London, 3St. Bartholomew's Hospital and The London NHS Trust


JoVE 3721

Date Published: March 22, 2012  |  doi:10.3791/3721  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

1Laboratory of Cellular and Molecular Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health


JoVE 3892

Date Published: March 22, 2012  |  doi:10.3791/3892  |  Section: JoVE Immunology and Infection

 JoVE Clinical and Translational Medicine

MRI-guided Disruption of the Blood-brain Barrier using Transcranial Focused Ultrasound in a Rat Model

1Imaging Research, Sunnybrook Research Institute, 2Department of Medical Biophysics, University of Toronto, 3Department of Medical Biophysics, and Institute of Biomaterials & Biomedical Engineering (IBBME), University of Toronto


JoVE 3555

Date Published: March 13, 2012  |  doi:10.3791/3555  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Clinical Testing and Spinal Cord Removal in a Mouse Model for Amyotrophic Lateral Sclerosis (ALS)

1Dept. of Neurology, University Medicine Göttingen, 2DFG Research Center for the Molecular Physiology of the Brain (CMPB), Göttingen, Germany


JoVE 3936

Date Published: March 17, 2012  |  doi:10.3791/3936  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Assessing Teratogenic Changes in a Zebrafish Model of Fetal Alcohol Exposure

1Program in Developmental Biology, Children's Memorial Research Center, 2Department of Pediatrics, Northwestern University


JoVE 3704

Date Published: March 20, 2012  |  doi:10.3791/3704  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Eye Tracking Young Children with Autism

1School of Behavioral and Brain Sciences, University of Texas at Dallas, 2Carolina Institute for Developmental Disabilities, School of Medicine, University of North Carolina at Chapel Hill


JoVE 3675

Date Published: March 27, 2012  |  doi:10.3791/3675  |  Section: JoVE Clinical and Translational Medicine

 JoVE Bioengineering

Preparation of 3D Fibrin Scaffolds for Stem Cell Culture Applications

1Department of Biology, University of Victoria, 2Department of Mechanical Engineering, Division of Medical Sciences, University of Victoria


JoVE 3641

Date Published: March 2, 2012  |  doi:10.3791/3641  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Alginate Microcapsule as a 3D Platform for Propagation and Differentiation of Human Embryonic Stem Cells (hESC) to Different Lineages

1Stem Cell Lab, School of Psychiatry, Faculty of Medicine, The University of New South Wales, 2Siriraj Center of Excellence for Stem cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University, 3Neuropsychiatric Institute, Prince of Wales Hospital


JoVE 3608

Date Published: March 9, 2012  |  doi:10.3791/3608  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells

1Department of Cell Biology, Center for Cell Dynamics, Johns Hopkins University, 2Graduate School of Pharmaceutical Sciences, University of Tokyo, 3Biomedical Engineering, Johns Hopkins University


JoVE 3794

Date Published: March 9, 2012  |  doi:10.3791/3794  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Monitoring the Reductive and Oxidative Half-Reactions of a Flavin-Dependent Monooxygenase using Stopped-Flow Spectrophotometry

1Department of Biochemistry, Virginia Polytechnic Institute and State University


JoVE 3803

Date Published: March 18, 2012  |  doi:10.3791/3803  |  Section: JoVE Bioengineering

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