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The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.

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Table of Contents

Table of Contents: Issue 50, April 2011

Jump to:  JoVE Editorial (1) JoVE General (18) JoVE Neuroscience (12) JoVE Immunology and Infection (9) JoVE Clinical and Translational Medicine (9) JoVE Bioengineering (3)
 JoVE General

Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney


JoVE 2555

1, 2, 1, 1, 1, 1, 1

1Department of Molecular Medicine, Maine Medical Center Research Institute, 2Molecular Medicine and Gene Therapy, Lund University Hospital

Date Published: April 22, 2011  |  doi:10.3791/2555  |  Section: JoVE General

Keywords: Cellular Biology, Kidney development, nephrogenesis, nephrogenic zone, nephron progenitor cells, cortical stroma

 JoVE General

Modified Mouse Embryonic Stem Cell based Assay for Quantifying Cardiogenic Induction Efficiency


JoVE 2656

1, 1, 1, 1,2,3,4

1Division of Cardiovascular Medicine, Department of Medicine, Vanderbilt University School of Medicine, 2Department of Pharmacology, Vanderbilt University School of Medicine, 3Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, 4Research Medicine, Veterans Administration TVHS

Date Published: April 22, 2011  |  doi:10.3791/2656  |  Section: JoVE General

Keywords: Cellular Biology, Embryonic stem cells (ES) cells, embryonic bodies (EB), signaling pathways, modulators, 96-round bottom well microtiter plates and hanging droplets.

 JoVE General

Modified Annexin V/Propidium Iodide Apoptosis Assay For Accurate Assessment of Cell Death


JoVE 2597

1, 1, 1, 1,2

1Department of Biological Sciences, University of Alberta, 2Department of Agriculture, Food and Nutrition Sciences, University of Alberta

Date Published: April 24, 2011  |  doi:10.3791/2597  |  Section: JoVE General

Keywords: Cellular Biology, Apoptosis, cell death, propidium iodide, Annexin V, necrosis, immunology

 JoVE General

Detection of Post-translational Modifications on Native Intact Nucleosomes by ELISA


JoVE 2593

1, 2, 3, 2, 3

1Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, 2Department of Molecular and Cell Biology, University of Connecticut, 3Department of Pharmaceutical Sciences, University of Connecticut

Date Published: April 26, 2011  |  doi:10.3791/2593  |  Section: JoVE General

Keywords: Cellular Biology, Chromatin, Nucleosome, Epigenetics, ELISA, Histone, Modification, Methylation, Acetylation

 JoVE General

iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution


JoVE 2638

1, 2, 3, 3, 1, 3, 4, 2, 1

1Laboratory of Molecular Biology, Medical Research Council - MRC, 2European Bioinformatics Institute, EMBL Heidelberg, 3Computer and Information Science, University of Ljubljana, 4Wellcome Trust Genome Campus, Wellcome Trust Sanger Institute

Date Published: April 30, 2011  |  doi:10.3791/2638  |  Section: JoVE General

Keywords: Cellular Biology, RNA biochemistry, transcriptome, systems biology, RNA-binding protein

 JoVE Neuroscience

Time-lapse Live Imaging of Clonally Related Neural Progenitor Cells in the Developing Zebrafish Forebrain


JoVE 2594

, ,

Department of Bioengineering and Therapeutic Sciences, Programs in Human Genetics and Biological Sciences , University of California San Francisco

Date Published: April 6, 2011  |  doi:10.3791/2594  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Live imaging, electroporation, confocal microscopy, neural progenitor cells, forebrain, zebrafish

 JoVE Neuroscience

Fluorescence Recovery After Photobleaching (FRAP) of Fluorescence Tagged Proteins in Dendritic Spines of Cultured Hippocampal Neurons


JoVE 2568

, , ,

National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda

Date Published: April 16, 2011  |  doi:10.3791/2568  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Spine, FRAP, hippocampal neurons, live cell imaging, protein mobility

 JoVE Neuroscience

Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation


JoVE 2678

1,2, 1

1Department of Neurobiology, Yale University, 2Program in Developmental Biology, Baylor College of Medicine

Date Published: April 17, 2011  |  doi:10.3791/2678  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Retinotopy, Eye Segregation, Superior Colliculus, Lateral Geniculate Nucleus, Visual Development, Retinal Ganglion Cell, Retina, Electroporation

 JoVE Neuroscience

An Optic Nerve Crush Injury Murine Model to Study Retinal Ganglion Cell Survival


JoVE 2685

1, 1,2, 1, 1, 1, 1, 1, 1

1National Eye Institute, NIH, 2Ophthalmology Department, The Second Hospital of Harbin Medical University

Date Published: April 25, 2011  |  doi:10.3791/2685  |  Section: JoVE Neuroscience

Keywords: Neuroscience, optic nerve crush injury, retinal ganglion cell, glaucoma, optic neuropathy, retrograde labeling

 JoVE Immunology and Infection

Quantitative Analyses of all Influenza Type A Viral Hemagglutinins and Neuraminidases using Universal Antibodies in Simple Slot Blot Assays


JoVE 2784

*1, *2, 2, 1,3,4, 1, 5, 5, 1,3

1Centre for Vaccine Evaluation, Biologics and Genetic Therapies Directorate, HPFB, Health canada, 2National Institute for the Control of Pharmaceutical and Biological Products, The State Food and Drug Administration, Beijing, 3Department of Biochemistry, Microbiology and Immunology, University of Ottawa, 4Microbiology Department, Faculty of Medicine, King Abdulaziz University, 5National Microbiology Laboratory, Public Health Agency of Canada

Date Published: April 4, 2011  |  doi:10.3791/2784  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Virology, influenza, hemagglutinin, neuraminidase, quantification, universal antibody

 JoVE Immunology and Infection

Quantitative Assessment of Immune Cells in the Injured Spinal Cord Tissue by Flow Cytometry: a Novel Use for a Cell Purification Method


JoVE 2698

1,2,3,4, 5, 1,2,3,4,6

1Institute for Memory Impairments and Neurological Disorders, University of California, 2Physical Medicine & Rehabilitation, University of California, 3Anatomy & Neurobiology, University of California, 4Sue and Bill Gross Stem Cell Research Center, University of California, 5Section of Molecular Biology, University of California, 6Reeve-Irvine Research Center, University of California

Date Published: April 9, 2011  |  doi:10.3791/2698  |  Section: JoVE Immunology and Infection

Keywords: Immunology, spinal cord injury, cellular inflammation, neuroinflammation, OptiPrep, central nervous system, neutrophils, macrophages, microglia, T-cells, flow cytometry

 JoVE Immunology and Infection

HLA-Ig Based Artificial Antigen Presenting Cells for Efficient ex vivo Expansion of Human CTL


JoVE 2801

1,2, 3,4, 3,4

1Immunology Graduate Program, Johns Hopkins University, 2Department of Internal Medicine, Far-Eastern Memorial Hospital, 3Department of Pathology, Johns Hopkins University, 4Institute of Cell Engineering, Johns Hopkins University

Date Published: April 11, 2011  |  doi:10.3791/2801  |  Section: JoVE Immunology and Infection

Keywords: Immunology, immunotherapy, adoptive T cell therapy, CD8+ T cells, HLA-A2-Ig, CMV, aAPC, DC

 JoVE Immunology and Infection

Using Bioluminescent Imaging to Investigate Synergism Between Streptococcus pneumoniae and Influenza A Virus in Infant Mice


JoVE 2357

1, 2, 1, 1, 3, 1, 1

1Department of Microbiology and Immunology, University of Melbourne, 2Laboratory of Pediatric Infectious Diseases, Radboud University Nijmegen Medical Centre, 3The Centre for Dynamic Imaging, The Walter and Eliza Hall Institute for Medical Research

Date Published: April 14, 2011  |  doi:10.3791/2357  |  Section: JoVE Immunology and Infection

Keywords: Infection, Bioluminescent imaging, influenza A virus, Streptococcus pneumoniae, mice, intranasal infection, otitis media, co-infections

 JoVE Immunology and Infection

A Calcium Bioluminescence Assay for Functional Analysis of Mosquito (Aedes aegypti) and Tick (Rhipicephalus microplus) G Protein-coupled Receptors


JoVE 2732

1, 1, 1,2, 1

1Department of Entomology, Texas A&M University (TAMU), 2Department of Molecular and Cellular Medicine, Texas A&M University (TAMU)

Date Published: April 20, 2011  |  doi:10.3791/2732  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Aequorin calcium reporter, coelenterazine, G protein-coupled receptor (GPCR), CHO-K1 cells, mammalian cell culture, neuropeptide SAR studies (SAR= structure-activity relationships), receptor-neuropeptide interaction, bioluminescence, drug discovery, semi-throughput screening in plates

 JoVE Immunology and Infection

Using a Pan-Viral Microarray Assay (Virochip) to Screen Clinical Samples for Viral Pathogens


JoVE 2536

1, 1, 1,2, 1,2

1Department of Laboratory Medicine, University of California, San Francisco, 2Division of Infectious Diseases, University of California, San Francisco

Date Published: April 27, 2011  |  doi:10.3791/2536  |  Section: JoVE Immunology and Infection

Keywords: Immunology, virus, microarray, Virochip, viral detection, genomics, clinical diagnostics, viral discovery, metagenomics, novel pathogen discovery

 JoVE Clinical and Translational Medicine

In Vivo Canine Muscle Function Assay


JoVE 2623

1, 2, 3

1Department of Neurology and Wake Forest Institute for Regenerative Medicine, Wake Forest University, 2Department of Human Nutrition, Foods and Exercise, Virginia Polytechnic Institute and State University, 3Departments of Pathology and Laboratory Medicine and Neurology and the Gene Therapy Center , University of North Carolina-Chapel Hill

Date Published: April 5, 2011  |  doi:10.3791/2623  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, dog, muscle strength, muscle force, exercise, eccentric contraction, muscle damage, stretch

 JoVE Clinical and Translational Medicine

Methods for ECG Evaluation of Indicators of Cardiac Risk, and Susceptibility to Aconitine-induced Arrhythmias in Rats Following Status Epilepticus


JoVE 2726

, ,

Department of Pharmacology and Toxicology, University of Utah

Date Published: April 5, 2011  |  doi:10.3791/2726  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, cardiac, seizure disorders, QTc, QTd, cardiac arrhythmias, rat

 JoVE Clinical and Translational Medicine

Manual Muscle Testing: A Method of Measuring Extremity Muscle Strength Applied to Critically Ill Patients


JoVE 2632

1, 1, 1,2, 2,3, 4, 1,2,3

1Outcomes After Critical Illness and Surgery (OACIS) Group, Division of Pulmonary and Critical Care Medicine, Johns Hopkins University, 2Critical Care Physical Medicine and Rehabilitation Program, Johns Hopkins Hospital, 3Department of Physical Medicine and Rehabilitation, Johns Hopkins University, 4Department of Rehabilitation Services, University of Maryland Medical System

Date Published: April 12, 2011  |  doi:10.3791/2632  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Muscle Strength, Critical illness, Intensive Care Units, Reproducibility of Results, Clinical Protocols.

 JoVE Clinical and Translational Medicine

A Method for Murine Islet Isolation and Subcapsular Kidney Transplantation


JoVE 2096

1, 1,2, 1,2,3

1Molecular and Cellular Biochemistry, Center for Molecular Neurobiology, The Ohio State University, 2Integrated Biomedical Science Graduate Program, The Ohio State University, 3Comprehensive Cancer Center, The Ohio State University

Date Published: April 13, 2011  |  doi:10.3791/2096  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, islet isolation, islet transplantation, diabetes, murine, pancreas

 JoVE Clinical and Translational Medicine

Monitoring Tumor Metastases and Osteolytic Lesions with Bioluminescence and Micro CT Imaging


JoVE 2775

, , , , ,

Imaging Biology Research and Development, Caliper Life Sciences

Date Published: April 14, 2011  |  doi:10.3791/2775  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, osteolytic lesions, micro CT, tumor, bioluminescence, in vivo, imaging, IVIS, luciferase, low dose, co-registration, 3D reconstruction

 JoVE Clinical and Translational Medicine

Pseudofracture: An Acute Peripheral Tissue Trauma Model


JoVE 2074

1, 2, 2, 1, 2, 1

1Department of Surgery, University of Pittsburgh, 2Department of Orthopedic Surgery, University of Aachen Medical Center

Date Published: April 18, 2011  |  doi:10.3791/2074  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Trauma, musculoskeletal, mouse, extremity, inflammation, immunosuppression, immune response.

 JoVE Clinical and Translational Medicine

Methods to Quantify Pharmacologically Induced Alterations in Motor Function in Human Incomplete SCI


JoVE 2148

1,2, 1, 1, 1,3

1Sensory Motor Performance Program, Rehabilitation Institute of Chicago, 2Department of Kinesiology and Nutrition, University of Illinois at Chicago, 3Department of Physical Therapy, University of Illinois at Chicago

Date Published: April 18, 2011  |  doi:10.3791/2148  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, spinal cord injury, spasticity, locomotion, strength, vector coding, biomechanics, reflex, serotonin, human, electromyography

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