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  JoVE Biology

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  JoVE Neuroscience

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  JoVE Immunology and Infection

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  JoVE Medicine

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  JoVE Bioengineering

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  JoVE Chemistry

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  JoVE Developmental Biology


Table of Contents

Table of Contents: Issue 50, April 2011

Jump to:  JoVE Editorial (1) JoVE Biology (18) JoVE Neuroscience (12) JoVE Immunology and Infection (9) JoVE Medicine (9) JoVE Bioengineering (3)
 JoVE Biology

Evisceration of Mouse Vitreous and Retina for Proteomic Analyses

1Omics Laboratory, University of Iowa, 2Ophthalmology and Visual Sciences, University of Iowa, 3Harkness Eye Institute, Columbia University College of Physicians and Surgeons

JoVE 2795

Date Published: April 3, 2011  |  doi:10.3791/2795  |  Section: JoVE Biology

 JoVE Biology

Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney

1Department of Molecular Medicine, Maine Medical Center Research Institute, 2Molecular Medicine and Gene Therapy, Lund University Hospital

JoVE 2555

Date Published: April 22, 2011  |  doi:10.3791/2555  |  Section: JoVE Biology

 JoVE Biology

Modified Mouse Embryonic Stem Cell based Assay for Quantifying Cardiogenic Induction Efficiency

1Division of Cardiovascular Medicine, Department of Medicine, Vanderbilt University School of Medicine, 2Department of Pharmacology, Vanderbilt University School of Medicine, 3Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, 4Research Medicine, Veterans Administration TVHS

JoVE 2656

Date Published: April 22, 2011  |  doi:10.3791/2656  |  Section: JoVE Biology

 JoVE Biology

Modified Annexin V/Propidium Iodide Apoptosis Assay For Accurate Assessment of Cell Death

1Department of Biological Sciences, University of Alberta, 2Department of Agriculture, Food and Nutrition Sciences, University of Alberta

JoVE 2597

Date Published: April 24, 2011  |  doi:10.3791/2597  |  Section: JoVE Biology

 JoVE Biology

Detection of Post-translational Modifications on Native Intact Nucleosomes by ELISA

1Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, 2Department of Molecular and Cell Biology, University of Connecticut, 3Department of Pharmaceutical Sciences, University of Connecticut

JoVE 2593

Date Published: April 26, 2011  |  doi:10.3791/2593  |  Section: JoVE Biology

 JoVE Biology

Lineage Labeling of Zebrafish Cells with Laser Uncagable Fluorescein Dextran

1Department of Biological Sciences, Vanderbilt University, 2Department of Chemical and Systems Biology, Stanford University

JoVE 2672

Date Published: April 28, 2011  |  doi:10.3791/2672  |  Section: JoVE Biology

 JoVE Biology

iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution

1Laboratory of Molecular Biology, Medical Research Council - MRC, 2European Bioinformatics Institute, EMBL Heidelberg, 3Computer and Information Science, University of Ljubljana, 4Wellcome Trust Genome Campus, Wellcome Trust Sanger Institute

JoVE 2638

Date Published: April 30, 2011  |  doi:10.3791/2638  |  Section: JoVE Biology

 JoVE Neuroscience

Time-lapse Live Imaging of Clonally Related Neural Progenitor Cells in the Developing Zebrafish Forebrain

1Department of Bioengineering and Therapeutic Sciences, Programs in Human Genetics and Biological Sciences , University of California San Francisco

JoVE 2594

Date Published: April 6, 2011  |  doi:10.3791/2594  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Fluorescence Recovery After Photobleaching (FRAP) of Fluorescence Tagged Proteins in Dendritic Spines of Cultured Hippocampal Neurons

1National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda

JoVE 2568

Date Published: April 16, 2011  |  doi:10.3791/2568  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation

1Department of Neurobiology, Yale University, 2Program in Developmental Biology, Baylor College of Medicine

JoVE 2678

Date Published: April 17, 2011  |  doi:10.3791/2678  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Migratory Behavior of Cells Generated in Ganglionic Eminence Cultures

1Dept. of Anatomy, Physiology and Genetics, Uniformed Services University, 2Neuroscience Program, Uniformed Services University

JoVE 2583

Date Published: April 21, 2011  |  doi:10.3791/2583  |  Section: JoVE Neuroscience

 JoVE Neuroscience

An Optic Nerve Crush Injury Murine Model to Study Retinal Ganglion Cell Survival

1National Eye Institute, NIH, 2Ophthalmology Department, The Second Hospital of Harbin Medical University

JoVE 2685

Date Published: April 25, 2011  |  doi:10.3791/2685  |  Section: JoVE Neuroscience

 JoVE Immunology and Infection

Intravital Microscopy of the Inguinal Lymph Node

1Interdisciplinary Science, University of Northern British Columbia, 2Northern Medical Program, University of Northern British Columbia

JoVE 2551

Date Published: April 4, 2011  |  doi:10.3791/2551  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Quantitative Analyses of all Influenza Type A Viral Hemagglutinins and Neuraminidases using Universal Antibodies in Simple Slot Blot Assays

1Centre for Vaccine Evaluation, Biologics and Genetic Therapies Directorate, HPFB, Health canada, 2National Institute for the Control of Pharmaceutical and Biological Products, The State Food and Drug Administration, Beijing, 3Department of Biochemistry, Microbiology and Immunology, University of Ottawa, 4Microbiology Department, Faculty of Medicine, King Abdulaziz University, 5National Microbiology Laboratory, Public Health Agency of Canada

JoVE 2784

Date Published: April 4, 2011  |  doi:10.3791/2784  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Quantitative Assessment of Immune Cells in the Injured Spinal Cord Tissue by Flow Cytometry: a Novel Use for a Cell Purification Method

1Institute for Memory Impairments and Neurological Disorders, University of California, 2Physical Medicine & Rehabilitation, University of California, 3Anatomy & Neurobiology, University of California, 4Sue and Bill Gross Stem Cell Research Center, University of California, 5Section of Molecular Biology, University of California, 6Reeve-Irvine Research Center, University of California

JoVE 2698

Date Published: April 9, 2011  |  doi:10.3791/2698  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

HLA-Ig Based Artificial Antigen Presenting Cells for Efficient ex vivo Expansion of Human CTL

1Immunology Graduate Program, Johns Hopkins University, 2Department of Internal Medicine, Far-Eastern Memorial Hospital, 3Department of Pathology, Johns Hopkins University, 4Institute of Cell Engineering, Johns Hopkins University

JoVE 2801

Date Published: April 11, 2011  |  doi:10.3791/2801  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Using Bioluminescent Imaging to Investigate Synergism Between Streptococcus pneumoniae and Influenza A Virus in Infant Mice

1Department of Microbiology and Immunology, University of Melbourne, 2Laboratory of Pediatric Infectious Diseases, Radboud University Nijmegen Medical Centre, 3The Centre for Dynamic Imaging, The Walter and Eliza Hall Institute for Medical Research

JoVE 2357

Date Published: April 14, 2011  |  doi:10.3791/2357  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

A Calcium Bioluminescence Assay for Functional Analysis of Mosquito (Aedes aegypti) and Tick (Rhipicephalus microplus) G Protein-coupled Receptors

1Department of Entomology, Texas A&M University (TAMU), 2Department of Molecular and Cellular Medicine, Texas A&M University (TAMU)

JoVE 2732

Date Published: April 20, 2011  |  doi:10.3791/2732  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Particle Agglutination Method for Poliovirus Identification

1Department of Virology II, National Institute of Infectious Diseases, 2New Product Design Department, Fujirebio Inc.

JoVE 2824

Date Published: April 20, 2011  |  doi:10.3791/2824  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Using a Pan-Viral Microarray Assay (Virochip) to Screen Clinical Samples for Viral Pathogens

1Department of Laboratory Medicine, University of California, San Francisco, 2Division of Infectious Diseases, University of California, San Francisco

JoVE 2536

Date Published: April 27, 2011  |  doi:10.3791/2536  |  Section: JoVE Immunology and Infection

 JoVE Medicine

In Vivo Canine Muscle Function Assay

1Department of Neurology and Wake Forest Institute for Regenerative Medicine, Wake Forest University, 2Department of Human Nutrition, Foods and Exercise, Virginia Polytechnic Institute and State University, 3Departments of Pathology and Laboratory Medicine and Neurology and the Gene Therapy Center , University of North Carolina-Chapel Hill

JoVE 2623

Date Published: April 5, 2011  |  doi:10.3791/2623  |  Section: JoVE Medicine

 JoVE Medicine

Manual Muscle Testing: A Method of Measuring Extremity Muscle Strength Applied to Critically Ill Patients

1Outcomes After Critical Illness and Surgery (OACIS) Group, Division of Pulmonary and Critical Care Medicine, Johns Hopkins University, 2Critical Care Physical Medicine and Rehabilitation Program, Johns Hopkins Hospital, 3Department of Physical Medicine and Rehabilitation, Johns Hopkins University, 4Department of Rehabilitation Services, University of Maryland Medical System

JoVE 2632

Date Published: April 12, 2011  |  doi:10.3791/2632  |  Section: JoVE Medicine

 JoVE Medicine

A Method for Murine Islet Isolation and Subcapsular Kidney Transplantation

1Molecular and Cellular Biochemistry, Center for Molecular Neurobiology, The Ohio State University, 2Integrated Biomedical Science Graduate Program, The Ohio State University, 3Comprehensive Cancer Center, The Ohio State University

JoVE 2096

Date Published: April 13, 2011  |  doi:10.3791/2096  |  Section: JoVE Medicine

 JoVE Medicine

Monitoring Tumor Metastases and Osteolytic Lesions with Bioluminescence and Micro CT Imaging

1Imaging Biology Research and Development, Caliper Life Sciences

JoVE 2775

Date Published: April 14, 2011  |  doi:10.3791/2775  |  Section: JoVE Medicine

 JoVE Medicine

Pseudofracture: An Acute Peripheral Tissue Trauma Model

1Department of Surgery, University of Pittsburgh, 2Department of Orthopedic Surgery, University of Aachen Medical Center

JoVE 2074

Date Published: April 18, 2011  |  doi:10.3791/2074  |  Section: JoVE Medicine

 JoVE Medicine

Methods to Quantify Pharmacologically Induced Alterations in Motor Function in Human Incomplete SCI

1Sensory Motor Performance Program, Rehabilitation Institute of Chicago, 2Department of Kinesiology and Nutrition, University of Illinois at Chicago, 3Department of Physical Therapy, University of Illinois at Chicago

JoVE 2148

Date Published: April 18, 2011  |  doi:10.3791/2148  |  Section: JoVE Medicine

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