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The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.

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Table of Contents

Table of Contents: Issue 53, July 2011

Jump to:  JoVE Editorial (1) JoVE General (18) JoVE Neuroscience (11) JoVE Immunology and Infection (11) JoVE Clinical and Translational Medicine (11) JoVE Bioengineering (4) JoVE Errata (1)
 JoVE General

Live Cell Calcium Imaging Combined with siRNA Mediated Gene Silencing Identifies Ca2+ Leak Channels in the ER Membrane and their Regulatory Mechanisms


JoVE 2730

*1, *1, 2, 1

1Medical Biochemistry and Molecular Biology, Saarland University, 2Experimental and Clinical Pharmacology and Toxicology, Saarland University

Date Published: July 7, 2011  |  doi:10.3791/2730  |  Section: JoVE General

Keywords: Cell Biology, Cellular calcium homeostasis, calmodulin, complementation, endoplasmic reticulum, ER calcium leakage, gene silencing, IQ motif, mutant analysis, Sec61 complex

 JoVE General

Bioengineering Human Microvascular Networks in Immunodeficient Mice


JoVE 3065

,

Department of Cardiac Surgery, Children's Hospital Boston, Harvard Medical School

Date Published: July 11, 2011  |  doi:10.3791/3065  |  Section: JoVE General

Keywords: Bioengineering, vascular network, blood vessel, vasculogenesis, angiogenesis, endothelial progenitor cells, endothelial colony-forming cells, mesenchymal stem cells, collagen gel, fibrin gel, tissue engineering, regenerative medicine

 JoVE General

Quantifying the Frequency of Tumor-propagating Cells Using Limiting Dilution Cell Transplantation in Syngeneic Zebrafish


JoVE 2790

1, 1, 2

1Department of Molecular Pathology, Massachusetts General Hospital, Harvard Medical School, 2Department of Molecular Pathology, Massachusetts General Hospital Cancer Center, Harvard Stem Cell Institute

Date Published: July 14, 2011  |  doi:10.3791/2790  |  Section: JoVE General

Keywords: Developmental Biology, cancer stem cell, T-cell acute lymphoblastic leukemia, microinjection, fluorescence, self-renewal

 JoVE General

Lentiviral-mediated Knockdown During Ex Vivo Erythropoiesis of Human Hematopoietic Stem Cells


JoVE 2813

1, 1, 1,2

1The Sprott Center for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, 2Department of Cellular and Molecular Medicine, University of Ottawa

Date Published: July 16, 2011  |  doi:10.3791/2813  |  Section: JoVE General

Keywords: Cellular Biology, Human hematopoietic stem cells, erythropoiesis, red blood cells, lentivirus, knockdown, transcription factor

 JoVE General

Visualization of the Interstitial Cells of Cajal (ICC) Network in Mice


JoVE 2802

1,2, 2, 3, 3, 2,4, 1,5

1Department of Medicine, Memorial Sloan Kettering Cancer Center, 2Human Oncology and Pathogenesis Program, Memorial Sloan Kettering Cancer Center, 3Developmental Biology Program, Memorial Sloan Kettering Cancer Center, 4Howard Hughes, Medical Institute, 5Laboratory of Chromatin Biology and Epigenetics, The Rockefeller University

Date Published: July 27, 2011  |  doi:10.3791/2802  |  Section: JoVE General

Keywords: Developmental Biology, mice, fluorescence microscopy, gastrointestinal track, motility, interstital cells of cajal, kit, ano1, pgp9.5

 JoVE General

Long-term Culture of Human Breast Cancer Specimens and Their Analysis Using Optical Projection Tomography


JoVE 3085

1, 2, 1, 2, 1, 1

1Breakthrough Breast Cancer Research Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, 2MRC Technology

Date Published: July 29, 2011  |  doi:10.3791/3085  |  Section: JoVE General

Keywords: Medicine, Breast cancer, Optical Projection Tomography, Imaging, Three-dimensional, computer assisted, Tumour microenvironment

 JoVE General

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry


JoVE 2812

*1, *1, 2, 3, 4, 5, 2, 3, 1

1Clinical Research Division, Fred Hutchinson Cancer Research Center - FHCRC, 2Department of Biochemistry and Microbiology, University of Victoria, 3Broad Institute of MIT and Harvard, 4Genome BC Proteomics Centre, University of Victoria, 5Plasma Proteome Institute

Date Published: July 31, 2011  |  doi:10.3791/2812  |  Section: JoVE General

Keywords: Molecular Biology, Mass spectrometry, targeted assay, peptide, MRM, SISCAPA, protein quantitation

 JoVE Neuroscience

Brain Imaging Investigation of the Neural Correlates of Observing Virtual Social Interactions


JoVE 2379

1, 2, 3, 3, 4, 5, 2,6,7

1Department of Computing Science, University of Alberta, 2Department of Psychology, University of Illinois, 3Centre for Neuroscience, University of Alberta, 4Department of Psychology, University of Alberta, 5Department of Marketing, Business Economics, and Law, University of Alberta, 6Neuroscience Program, University of Illinois at Urbana-Champaign, 7Beckman Institute, University of Illinois at Urbana-Champaign

Date Published: July 6, 2011  |  doi:10.3791/2379  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Social Perception, Social Knowledge, Social Cognition Network, Non-Verbal Communication, Decision-Making, Event-Related fMRI

 JoVE Neuroscience

Surgical Transplantation of Mouse Neural Stem Cells into the Spinal Cords of Mice Infected with Neurotropic Mouse Hepatitis Virus


JoVE 2834

1,2, 1,2, 1,2, 1,2, 1,2,3

1Department of Molecular Biology and Biochemistry, University of California, Irvine, 2Sue and Bill Gross Stem Cell Center, University of California, Irvine, 3Institute for Immunology, University of California, Irvine

Date Published: July 10, 2011  |  doi:10.3791/2834  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Transplantation, neural stem cells, spinal cord, laminectomy, demyelination, virus

 JoVE Neuroscience

Analysis of Dendritic Spine Morphology in Cultured CNS Neurons


JoVE 2794

1, 1, 1,2

1Department of Physiology, Northwestern University Feinberg School of Medicine, 2Department of Psychiatry and Behavioral Sciences, Northwestern University Feinberg School of Medicine

Date Published: July 13, 2011  |  doi:10.3791/2794  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Excitatory synapse, neuroscience, brain, cortex, cortical neurons, primary culture, confocal microscopy, time-lapse imaging, remodeling.

 JoVE Neuroscience

Screening Assay for Oxidative Stress in a Feline Astrocyte Cell Line, G355-5


JoVE 2841

1, 1, 1, 2, 3, 2, 1,2

1College of Veterinary Medicine, Western University of Health Sciences, 2Graduate College of Biomedical Sciences, Western University of Health Sciences, 3ReadiSorb, Products

Date Published: July 13, 2011  |  doi:10.3791/2841  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Astrocytes, oxidative stress, flow cytometry, CM-H2DCFDA

 JoVE Neuroscience

Chromatin Immunoprecipitation from Dorsal Root Ganglia Tissue following Axonal Injury


JoVE 2803

*1,2, *1, 1

1Laboratory for NeuroRegeneration and Repair, Department of Neurology, Hertie Institute for Clinical Brain Research, University of Tuebingen, 2Graduate School for Cellular and Molecular Neuroscience, University of Tuebingen

Date Published: July 20, 2011  |  doi:10.3791/2803  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Chromatin immunoprecipitation, dorsal root ganglia, transcription factor, epigenetic, axonal regeneration

 JoVE Neuroscience

A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices


JoVE 2807

1, 2, 3,4

1Department of Molecular & Human Genetics, Baylor College of Medicine (BCM), 2Precisionary Instruments Inc., 3Departments of Molecular & Human Genetics and Neuroscience, Baylor College of Medicine (BCM), 4Jan and Dan Duncan Neurological Research Institute, Texas Children's Hospital

Date Published: July 26, 2011  |  doi:10.3791/2807  |  Section: JoVE Neuroscience

Keywords: Neuroscience, nervous tissue, neurons, confocal, epifluorescent, imaging, clearing, fluorescent proteins

 JoVE Immunology and Infection

Immuno-fluorescence Assay of Leptospiral Surface-exposed Proteins


JoVE 2805

1,2, 3,4

1Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, 2Research service, 151, Veterans Affairs Greater Los Angeles Healthcare System, 3Departments of Medicine, Urology at David Geffen School of Medicine and Department of Microbiology, Immunology and Molecular Gentics, University of California Los Angeles (UCLA), 4Division of Infectious Diseases, 111F, Veterans Affairs Greater Los Angeles Health Care System

Date Published: July 1, 2011  |  doi:10.3791/2805  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Molecular Biology, Leptospira, intact cells, outer membrane, surface-exposed proteins, surface immuno-fluorescence

 JoVE Immunology and Infection

Flow Cytometry Analysis of Immune Cells Within Murine Aortas


JoVE 2848

1, 1, 2, 1

1Deptartment of Microbiology and Molecular Cell Biology, Eastern Virginia Medical School, 2Division of Inflammation Biology, LaJolla Institute for Allergy and Immunology

Date Published: July 1, 2011  |  doi:10.3791/2848  |  Section: JoVE Immunology and Infection

Keywords: Immunology, atherosclerosis, immune response, leukocytes, adventitia, flow cytometry

 JoVE Immunology and Infection

'Bioluminescent' Reporter Phage for the Detection of Category A Bacterial Pathogens


JoVE 2740

1, 2, 3

1BioSciences Division, Guild Associates, Inc., 2Department of Molecular Genetics and Microbiology, University of Texas at Austin, 3Department of Craniofacial Biology, Medical University of South Carolina

Date Published: July 8, 2011  |  doi:10.3791/2740  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Reporter phage, bioluminescence, detection, plague, anthrax

 JoVE Immunology and Infection

Visualizing Dengue Virus through Alexa Fluor Labeling


JoVE 3168

1, 2, 3

1Defence Medical and Environmental Research Institute, DSO National Laboratories, 2Program in Emerging Infectious Diseases, Duke-NUS Graduate Medical School, 3Program in Emerging Infectious Diseases, Duke-NUS Graduate Medical School

Date Published: July 9, 2011  |  doi:10.3791/3168  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Dengue virus, Alexa Fluor, labeling, fluorescence

 JoVE Immunology and Infection

Ex vivo Imaging of T Cells in Murine Lymph Node Slices with Widefield and Confocal Microscopes


JoVE 3054

1,2, 1,2, 1,2, 1,2, 1,2, 1,2

1Institut Cochin, Université Paris Descartes, CNRS (UMR 8104), 2Inserm, U1016, Paris, France

Date Published: July 15, 2011  |  doi:10.3791/3054  |  Section: JoVE Immunology and Infection

Keywords: Immunology, mouse, lymph node, organotypic slices, T cell, migration, fluorescence, microscopy, confocal

 JoVE Immunology and Infection

DNA Fingerprinting of Mycobacterium leprae Strains Using Variable Number Tandem Repeat (VNTR) - Fragment Length Analysis (FLA)


JoVE 3104

, , ,

Department of Microbiology, Immunology and Pathology, Colorado State University

Date Published: July 15, 2011  |  doi:10.3791/3104  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Mycobacterium leprae, leprosy, biopsy, STR, VNTR, PCR, fragment length analysis

 JoVE Immunology and Infection

Human In Vitro Suppression as Screening Tool for the Recognition of an Early State of Immune Imbalance


JoVE 3071

1, 2, 3

1Department of Pediatrics/Allergy, Medical College of Wisconsin, 2Flow Cytometry Core Facility, Medical College of Wisconsin, 3Max McGee National Research Center for Juvenile Diabetes and Human Molecular Genetics Center, Medical College of Wisconsin

Date Published: July 22, 2011  |  doi:10.3791/3071  |  Section: JoVE Immunology and Infection

Keywords: Immunology, suppression, regulatory T cells, Tregs, activated T cells, autoimmune disease, Type 1 Diabetes (T1D)

 JoVE Immunology and Infection

Use of an Optical Trap for Study of Host-Pathogen Interactions for Dynamic Live Cell Imaging


JoVE 3123

1, 2, 3, 1, 1, 3, 4, 1

1Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Harvard Medical School, 2Department of Mechanical and Aerospace Engineering, The Ohio State University, 3Center for Computational and Integrative Biology, Massachusetts General Hospital, Harvard Medical School, 4Dept. of Chemical and Biomolecular Engineering, Vanderbilt University

Date Published: July 28, 2011  |  doi:10.3791/3123  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Optical trapping, optical tweezers, T-cell, pathogen, live cell imaging, spinning disk confocal microscopy, Aspergillus fumigatus, Candida albicans, fungi

 JoVE Immunology and Infection

Live Cell Imaging of Bacillus subtilis and Streptococcus pneumoniae using Automated Time-lapse Microscopy


JoVE 3145

, , ,

Molecular Genetics Group, Groningen Biomolecular Sciences and Biotechnology Institute, Centre for Synthetic Biology, University of Groningen

Date Published: July 28, 2011  |  doi:10.3791/3145  |  Section: JoVE Immunology and Infection

Keywords: Immunology, time-lapse fluorescence microscopy, single cell analysis, cell history, cell growth, development, promoter activity, protein localization, GFP, Bacillus subtilis, Streptococcus pneumoniae

 JoVE Clinical and Translational Medicine

Microvascular Decompression: Salient Surgical Principles and Technical Nuances


JoVE 2590

1, *2, *2, *1, *1

1Department of Neurosurgery, Vanderbilt University Medical Center, 2Vanderbilt School of Medicine, Vanderbilt University Medical Center

Date Published: July 5, 2011  |  doi:10.3791/2590  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, microvascular, decompression, trigeminal, neuralgia, operation, video

 JoVE Clinical and Translational Medicine

Electrolytic Inferior Vena Cava Model (EIM) of Venous Thrombosis


JoVE 2737

1, 1, 1, 2, 1, 1

1Conrad Jobst Vascular Research Laboratories, Section of Vascular Surgery, University of Michigan, 2Department of Pharmacology, University of Michigan

Date Published: July 12, 2011  |  doi:10.3791/2737  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Endothelial dysfunction, Thrombosis, Electrolytic injury, Inflammation, Animal model

 JoVE Clinical and Translational Medicine

Use of a Hanging-weight System for Isolated Renal Artery Occlusion


JoVE 2549

1, 1, 1, 1, 2, 3, 1

1Department of Anesthesiology, University of Colorado, 2School of Medicine, University of Colorado, 3Department of Anesthesiology, Korea University College of Medicine

Date Published: July 19, 2011  |  doi:10.3791/2549  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, targeted gene deletion, murine model, acute renal failure, ischemia, reperfusion, video demonstration

 JoVE Clinical and Translational Medicine

Multi-photon Imaging of Tumor Cell Invasion in an Orthotopic Mouse Model of Oral Squamous Cell Carcinoma


JoVE 2941

1, 1, 1, 2, 3, 1

1Department of Neurobiology and Anatomy, Program in Cancer Cell Biology, Mary Babb Randolph Cancer Center, West Virginia University, 2Sensory Neuroscience Research Center, West Virginia University, 3Departments of Otolaryngology and Physiology, Center for Neuroscience, West Virginia University

Date Published: July 25, 2011  |  doi:10.3791/2941  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Invasion, mouse model, two-photon microscopy, tongue, orthotopic, head and neck cancer

 JoVE Clinical and Translational Medicine

The Application Of Permanent Middle Cerebral Artery Ligation in the Mouse


JoVE 3039

1, 2, 3

1Department of Pharmacology and Physiology, University of Rochester, 2Department of Neurology, University of Alabama at Birmingham, 3Departments of Anesthesiology, Pharmacology and Physiology, University of Rochester

Date Published: July 25, 2011  |  doi:10.3791/3039  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, brain, stroke, mouse, middle cerebral artery ligation

 JoVE Clinical and Translational Medicine

Method for Novel Anti-Cancer Drug Development using Tumor Explants of Surgical Specimens


JoVE 2846

1, 1, 1, 1, 2, 1

1Department of Neurological Surgery, The Ohio State University Medical Center, 2Department of Pathology, The Ohio State University Medical Center

Date Published: July 29, 2011  |  doi:10.3791/2846  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Glioblastoma multiforme, glioma, temozolomide, therapeutics, drug design

 JoVE Clinical and Translational Medicine

High-Resolution Endocardial and Epicardial Optical Mapping in a Sheep Model of Stretch-Induced Atrial Fibrillation


JoVE 3103

, , , , , , , ,

Center for Arrhythmia Research. Internal Medicine, University of Michigan

Date Published: July 29, 2011  |  doi:10.3791/3103  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, atrial fibrillation, endocardial mapping, patterns of activation, posterior left atrium

 JoVE Clinical and Translational Medicine

Isolation of Human Islets from Partially Pancreatectomized Patients


JoVE 2962

1, 1, 1, 2, 1, 1, 3, 2, 1

1Department of GI-, Thorax- and Vascular Surgery, University Hospital Carl Gustav Carus, University of Technology Dresden, 2Molecular Diabetology, Paul Langerhans Institute Dresden, 3Department of Pathology, University Hospital Carl Gustav Carus, University of Technology Dresden

Date Published: July 30, 2011  |  doi:10.3791/2962  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, human islets, Diabetes mellitus, partial pancreatectomy, human islet isolation

 JoVE Bioengineering

Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems


JoVE 2896

1, 1, 2

1Bioengineering Department, Northeastern University, 2Department of Pharmaceutical Sciences, Northeastern University

Date Published: July 4, 2011  |  doi:10.3791/2896  |  Section: JoVE Bioengineering

Keywords: Bioengineering, nanoparticles, nanosensors, polymer, fluorescence, imaging, intracellular, in vivo, sodium

 JoVE Bioengineering

Increasing cDNA Yields from Single-cell Quantities of mRNA in Standard Laboratory Reverse Transcriptase Reactions using Acoustic Microstreaming


JoVE 3144

1, 2, 2, 3, 1, 1

1Florey Neuroscience Institutes and Centre for Neuroscience, University of Melbourne, 2Fluid Dynamics Group, CSIRO Materials Science and Engineering, 3Swinburne University of Technology, Faculty of Engineering and Industrial Sciences

Date Published: July 11, 2011  |  doi:10.3791/3144  |  Section: JoVE Bioengineering

Keywords: Bioengineering, neuroscience, brain, cells, reverse transcription, qPCR, gene expression, acoustic microstreaming, micromixer, microfluidics

 JoVE Bioengineering

Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy


JoVE 3061

1, 2, 2

1College of Nursing, Interdisciplinary Life Sciences Research Laboratory, Seattle University, 2College of Science and Engineering, Interdisciplinary Life Sciences Research Laboratory, Seattle University

Date Published: July 18, 2011  |  doi:10.3791/3061  |  Section: JoVE Bioengineering

Keywords: Bioengineering, atomic force microscopy, glucocorticoid receptor, protein-protein interaction, DNA-protein interaction, scanning probe microscopy, immunoadsorption

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