- 70: December 2012
- 69: November 2012
- 68: October 2012
- 67: September 2012
- 66: August 2012
- 65: July 2012
- 64: June 2012
- 63: May 2012
- 62: April 2012
- 61: March 2012
- 60: February 2012
- 59: January 2012
- 58: December 2011
- 57: November 2011
- 56: October 2011
- 55: September 2011
- 54: August 2011
- 53: July 2011
- 52: June 2011
- 51: May 2011
- 50: April 2011
- 49: March 2011
- 48: February 2011
- 47: January 2011
- 46: December 2010
- 45: November 2010
- 44: October 2010
- 43: September 2010
- 42: August 2010
- 41: July 2010
- 40: June 2010
- 39: May 2010
- 38: April 2010
- 37: March 2010
- 36: February 2010
- 35: January 2010
- 34: December 2009
- 33: November 2009
- 32: October 2009
- 31: September 2009
- 30: August 2009
- 29: July 2009
- 28: June 2009
- 27: May 2009
- 26: April 2009
- 25: March 2009
- 24: February 2009
- 23: January 2009
- 22: December 2008
- 21: November 2008
- 20: October 2008
- 19: September 2008
- 18: August 2008
- 17: July 2008
- 16: June 2008
- 15: May 2008
- 14: April 2008
- 13: March 2008
- 12: February 2008
- 11: January 2008
- 10: December 2007
- 9: November 2007
- 8: October 2007
- 7: August/September 2007
- 6: July/August 2007
- 5: June 2007
- 4: May 2007
- 3: April 2007
- 2: December-February 2006-07
Table of Contents
Table of Contents: Issue 30, August 2009
Fast and Sensitive Colloidal Coomassie G-250 Staining for Proteins in Polyacrylamide Gels
Biological Medical Research Center (BMFZ), University Duesseldorf
Date Published: August 3, 2009 | doi:10.3791/1431 | Section: JoVE General
Keywords: Basic Protocols, protein in-gel detection, Coomassie G-250, colloidal staining, 1- and 2-dimensional SDS-gels
Use of the Protease Fluorescent Detection Kit to Determine Protease Activity
Date Published: August 4, 2009 | doi:10.3791/1514 | Section: JoVE General
Keywords: Basic Protocols, Protease Fluorescent Detection Kit, Protease Detection, serine proteases, cysteine proteases, metallo-proteases, aspartic proteases
Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)
Department of Cell Biology, Scripps Institute
Date Published: August 5, 2009 | doi:10.3791/1325 | Section: JoVE General
Keywords: Cellular Biology, FSM, qFSM, speckle, actin, cytoskeleton, fluorescence, microscopy, microinjection
Obtaining High Quality RNA from Single Cell Populations in Human Postmortem Brain Tissue
1Department of Structural and Molecular Neuroscience, McLean Hospital, 2Department of Psychiatry, Harvard Medical School, 3Department of Psychiatry, Beth Israel Deaconess Medical Center
Date Published: August 6, 2009 | doi:10.3791/1444 | Section: JoVE General
Keywords: Neuroscience, Postmortem, microarrays, RNA, superior temporal gyrus, laser-capture microdissection, pyramidal neurons
RNA Isolation from Embryonic Zebrafish and cDNA Synthesis for Gene Expression Analysis
School of Health Sciences, Purdue University
Date Published: August 7, 2009 | doi:10.3791/1470 | Section: JoVE General
Keywords: Developmental Biology, zebrafish, RNA, cDNA, expression, microarray, gene
Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform
School of Health Sciences, Purdue University
Date Published: August 10, 2009 | doi:10.3791/1471 | Section: JoVE General
Keywords: Developmental Biology, zebrafish, microarray, genomics, gene expression, RNA, oligonucleotide
Optical Mapping of Langendorff-perfused Rat Hearts
1Department of Anesthesiology, Perioperative and Pain Medicine, Children's Hospital Boston and Harvard Medical School, 2Departments of Cardiac Surgery, Children's Hospital Boston and Harvard Medical School
Date Published: August 11, 2009 | doi:10.3791/1138 | Section: JoVE General
Keywords: Cellular Biology, cardiac, voltage-sensitive dye, electrophysiology, fluorescence, action potentials, crystalloid-perfusion
Mating and Tetrad Separation of Chlamydomonas reinhardtii for Genetic Analysis
Boyce Thompson Institute for Plant Research, Cornell University
Date Published: August 12, 2009 | doi:10.3791/1274 | Section: JoVE General
Keywords: Plant Biology, mating, zygote, tetrad, mating type, Chlamydomonas
A Technique to Simultaneously Visualize Virus-Specific CD8+ T Cells and Virus-Infected Cells In situ
1Department of Microbiology, Medical School, University of Minnesota, 2Department of Veterinary and Biomedical Sciences, University of Minnesota
Date Published: August 13, 2009 | doi:10.3791/1561 | Section: JoVE General
Keywords: Immunology, Virus, antigen-specific T cells, in situ tetramer staining, in situ hybridization
Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid
Protein Expression and Purification Core Facility, EMBL Heidelberg
Date Published: August 14, 2009 | doi:10.3791/1350 | Section: JoVE General
Keywords: Basic Protocols, SDS-PAGE, Coomassie staining, Protein detection, Protein staining
Isolation of Protoplasts from Tissues of 14-day-old Seedlings of Arabidopsis thaliana
Crop and Soil Sciences, Cornell University
Date Published: August 17, 2009 | doi:10.3791/1149 | Section: JoVE General
Keywords: Plant Biology, protoplasts, isolation, Arabidopsis, seedlings
In vivo Ca2+- Imaging of Mushroom Body Neurons During Olfactory Learning in the Honey Bee
1Institut für Biologie - Neurobiologie, Freie Universität Berlin, 2Institut für Biologie - Neurobiologie, Free University Berlin - Freie Universitaet Berlin
Date Published: August 18, 2009 | doi:10.3791/1353 | Section: JoVE General
Keywords: Neuroscience, Calcium Imaging, Insects, Mushroom Body, PER Conditioning, Olfaction, Fura-2
Detection of Protein Ubiquitination
Signal Transduction Program, The Sanford Burnham Institute for Medical Research
Date Published: August 19, 2009 | doi:10.3791/1293 | Section: JoVE General
Keywords: Cell Biology, Biochemistry, ubiquitination, cultured cell, in vitro system, immunoprecipitation, immunoblotting, ubiquitin, posttranslational modification
Mesoscopic Fluorescence Tomography for In-vivo Imaging of Developing Drosophila
1Center for Systems Biology, Massachusetts General Hospital, 2Institute for Biological and Medical Imaging (IBMI), Technical University of Munich and Helmholtz Center Munich, 3Department of Genetics, Harvard Medical School and Howard Hughes Medical Institute
Date Published: August 20, 2009 | doi:10.3791/1510 | Section: JoVE General
Keywords: Developmental Biology, fluorescence tomography, mesoscopic imaging, Drosophila, optical imaging, diffusion tomography, scattering
A Quantitative Assessment of The Yeast Lipidome using Electrospray Ionization Mass Spectrometry
Department of Biology, Concordia University
Date Published: August 21, 2009 | doi:10.3791/1513 | Section: JoVE General
Keywords: Cellular Biology, mass spectrometry, lipidomics, lipid identification, lipid quantification
Purification of Mitochondria from Yeast Cells
Department of Biology, Concordia University
Date Published: August 24, 2009 | doi:10.3791/1417 | Section: JoVE General
Keywords: Cellular Biology, subcellular fractionation, organelles, organelle purification, mitochondria
Antifouling Self-assembled Monolayers on Microelectrodes for Patterning Biomolecules
1Department of Physics, Texas A&M University (TAMU), 2Department of Biomedical Engineering, Texas A&M University (TAMU)
Date Published: August 25, 2009 | doi:10.3791/1390 | Section: JoVE General
Keywords: Biomedical Engineering, protein patterning, self-assembly, tubulin, kinesin, biofouling, bioNEMS, biosensor
Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
1Mechanical Engineering, Johns Hopkins University, 2Biomedical Engineering, Duke University, 3Biomedical Engineering, Johns Hopkins University
Date Published: August 26, 2009 | doi:10.3791/1432 | Section: JoVE General
Keywords: Biomedical Engineering, microfluidics, gene delivery, quantum dots, fluorescence resonance energy transfer, self-assembly, nanocomplexes
In vivo Imaging of Deep Cortical Layers using a Microprism
Department of Biomedical Engineering, Yale University
Date Published: August 27, 2009 | doi:10.3791/1509 | Section: JoVE General
Keywords: Neuroscience, Cortex, Layer V, Multiphoton Microscopy, Brain, Mouse, Fluorescence, Microprism, Imaging, Neurovasculature, In vivo
Bioluminescence Imaging of Heme Oxygenase-1 Upregulation in the Gua Sha Procedure
1Department of Radiology, Massachusetts General Hospital, Harvard Medical School, 2Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School, 3Gastrointestinal Unit, Massachusetts General Hospital, Harvard Medical School, 4Department of Medicine, Massachusetts General Hospital, Harvard Medical School, 5Center for biotechnology and Informatics, The Methodist Hospital Research Institute, 6Department of Radiology, The Methodist Hospital, Weill Cornell Medical College, 7Bejing University of Chinese Medicine, 8Department of Health Technology and Informatics, The Hong Kong Polytechnic University, 9Department of Radiology, Brigham and Women's Hospital, Harvard Medical School
Date Published: August 28, 2009 | doi:10.3791/1385 | Section: JoVE General
Keywords: Medicine, Gua Sha, blood extravasation, bruises, heme oxygenase-1, gene expression, systems biology, small animal molecular imaging, optical and bioluminescence imaging, HO-1-luciferase transgenic mice, Chinese folk therapy
Dopamine Release at Individual Presynaptic Terminals Visualized with FFNs
1Departments of Neurology, Columbia University, 2Departments of Psychiatry and Pharmacology, Columbia University, 3Department of Chemistry, Columbia University, 4eMolecules, Inc., 5Departments of Neurology and Physiology, University of California School of Medicine, San Francisco, 6Division of Molecular Therapeutics, New York Psychiatric Institute
Date Published: August 31, 2009 | doi:10.3791/1562 | Section: JoVE General
Keywords: Neuroscience, striatal slice, dopamine, synapse, synaptic vesicles, amphetamine, optical imaging, fluorescence, release
