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The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.

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Table of Contents

Table of Contents: Issue 58, December 2011

Jump to:  JoVE Editorial (1) JoVE General (13) JoVE Neuroscience (7) JoVE Immunology and Infection (9) JoVE Clinical and Translational Medicine (6) JoVE Bioengineering (9)
 JoVE General

Quantitative Comparison of cis-Regulatory Element (CRE) Activities in Transgenic Drosophila melanogaster


JoVE 3395

1, 2

1Department of Biology, University of Dayton, 2Department of Biology, Center for Tissue Regeneration and Engineering at Dayton, University of Dayton

Date Published: December 19, 2011  |  doi:10.3791/3395  |  Section: JoVE General

Keywords: Developmental Biology, Cis-regulatory element, CRE, cis-regulatory module, enhancer, site-specific integration, reporter transgenes, confocal microscopy, regulatory logic, transcription factors, binding sites, Drosophila melanogaster, Drosophila

 JoVE General

Visualization of Vascular Ca2+ Signaling Triggered by Paracrine Derived ROS


JoVE 3511

1, 1, 2, 1

1Department of Biochemistry, Temple University, 2Department of Anesthesiology and Pain Medicine, University of Washington

Date Published: December 21, 2011  |  doi:10.3791/3511  |  Section: JoVE General

Keywords: Molecular Biology, Reactive oxygen species, Calcium, paracrine superoxide, endothelial cells, confocal microscopy

 JoVE General

Quantifying Agonist Activity at G Protein-coupled Receptors


JoVE 3179

1, 2, 3

1Department of Pharmacology, University of California, Irvine, 2Department of Pharmacology, University of California, 3Schmid College of Science, Chapman University

Date Published: December 26, 2011  |  doi:10.3791/3179  |  Section: JoVE General

Keywords: Molecular Biology, agonist activity, active state, ligand bias, constitutive activity, G protein-coupled receptor

 JoVE General

In vivo Electroporation of Morpholinos into the Adult Zebrafish Retina


JoVE 3603

1, 2,3, 2,3

1Departments of Anatomy and Cell Biology and Ophthalmology, Wayne State University School of Medicine, 2Department of Biological Sciences, University of Notre Dame, 3Center for Zebrafish Research, University of Notre Dame

Date Published: December 27, 2011  |  doi:10.3791/3603  |  Section: JoVE General

Keywords: Developmental Biology, Electroporation, morpholino, zebrafish, retina, regeneration

 JoVE General

Two Types of Assays for Detecting Frog Sperm Chemoattraction


JoVE 3407

1, 2, 2

1Department of Animal Sciences, University of Illinois, Urbana-Champaign, 2School of Life Sciences, Arizona State University

Date Published: December 27, 2011  |  doi:10.3791/3407  |  Section: JoVE General

Keywords: Developmental Biology, Sperm chemotaxis, fertilization, sperm accumulation assay, sperm tracking assay, sperm motility, Xenopus laevis, egg jelly

 JoVE Neuroscience

Imaging Pheromone Sensing in a Mouse Vomeronasal Acute Tissue Slice Preparation


JoVE 3311

1, 1, 1, 2, 1

1Department of Pharmacology and Toxicology, University of Lausanne, 2Department of Genetics and Evolution, University of Geneva

Date Published: December 6, 2011  |  doi:10.3791/3311  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Vomeronasal organ, VNO, pheromone, calcium imaging, tissue slice preparation, floating immunohistochemistry, GFP

 JoVE Neuroscience

Imaging Neuronal Responses in Slice Preparations of Vomeronasal Organ Expressing a Genetically Encoded Calcium Sensor


JoVE 3404

1, 1, 1, 1, 1, 1,2

1Stowers Institute for Medical Research, 2Department of Anatomy and Cell Biology, The University of Kansas School of Medicine

Date Published: December 6, 2011  |  doi:10.3791/3404  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Vomeronasal organ, VNO, pheromone, urine, slice preparation, G-CaMP2, calcium imaging

 JoVE Neuroscience

Electrophysiological Measurements and Analysis of Nociception in Human Infants


JoVE 3118

*1, *2, 1, 1, 1, 3, 2, 1,4

1Neuroscience, Physiology and Pharmacology, University College London, 2Department of Clinical Neurophysiology, Great Ormond Street Hospital, 3Elizabeth Garrett Anderson Obstetric Hospital, University College Hospital, 4Nuffield Department of Anaesthetics, University of Oxford

Date Published: December 20, 2011  |  doi:10.3791/3118  |  Section: JoVE Neuroscience

Keywords: Neuroscience, pain, infant, electrophysiology, human development

 JoVE Neuroscience

Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic in vivo Optogenetic Stimulation


JoVE 3380

, , , ,

Laboratory for Perception and Memory, Institut Pasteur and Centre National de la Recherche Scientifique (CNRS)

Date Published: December 28, 2011  |  doi:10.3791/3380  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Olfactory bulb, Olfactory neurons, in vivo, viral transduction, mouse, LED

 JoVE Immunology and Infection

Determination of Molecular Structures of HIV Envelope Glycoproteins using Cryo-Electron Tomography and Automated Sub-tomogram Averaging


JoVE 2770

1,2, 1, 3, 3, 1, 1, 1, 1, 1, 1,4, 5, 6, 7, 8, 9, 10, 1, 11, 12, 1

1Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 2The Medical Research Council Mitochondrial Biology Unit, University of Cambridge, 3National Library of Medicine, National Institutes of Health, 4Massachusetts Institute of Technology, 5William Fremd High School, 6University of Virginia, 7Duke University, 8Yale University, 9University of Notre Dame, 10Washington University in St. Louis, 11Bioinformatics and Computational Biosciences Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 12Thomas Jefferson High School for Science and Technology

Date Published: December 1, 2011  |  doi:10.3791/2770  |  Section: JoVE Immunology and Infection

Keywords: Immunology, HIV, Envelope glycoprotein, membrane protein, vaccine design, cryo-electron tomography, transmission electron microscopy, structural biology, high school science, scientific outreach, scientific visualization, National Institutes of Health, National Cancer Institute, National Library of Medicine

 JoVE Immunology and Infection

Prediction of HIV-1 Coreceptor Usage (Tropism) by Sequence Analysis using a Genotypic Approach


JoVE 3264

1, 1, 1, 2, 1, 1, 3, 4, 5, 6, 1, 7, 2

1Institute of Virology, University of Cologne, 2Max Planck Institute for Informatics, 3Institute for Immune genetics, 4Department of Gastroenterology, Hepatology and Infectiology, University of Duesseldorf, 5Department of Dermatology, University of Essen, 6Department of Internal Medicine, University of Cologne, 7Augustinerinnen Hospital

Date Published: December 1, 2011  |  doi:10.3791/3264  |  Section: JoVE Immunology and Infection

Keywords: Immunology, HIV-1, coreceptor, coreceptor antagonist, prediction of coreceptor usage, tropism, R5, X4, maraviroc, MVC

 JoVE Immunology and Infection

Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses


JoVE 2820

, , , , ,

Laboratory of Water and Food Viral Pollution, Department of Microbiology, Faculty of Biology, University of Barcelona

Date Published: December 3, 2011  |  doi:10.3791/2820  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Quantitative PCR, qPCR, flocculation, virus, adenovirus, polyomavirus, water, Microbial Source Tracking, bovine, human, porcine, contamination

 JoVE Immunology and Infection

Recognition of Epidermal Transglutaminase by IgA and Tissue Transglutaminase 2 Antibodies in a Rare Case of Rhesus Dermatitis


JoVE 3154

1, 1, 2, 3, 2, 2

1Division of Microbiology, Tulane National Primate Research Center, 2Division of Comparative Pathology, Tulane National Primate Research Center, 3Division of Veterinary Medicine, Tulane National Primate Research Center

Date Published: December 15, 2011  |  doi:10.3791/3154  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Gluten sensitivity, transglutaminase, autoimmunity, dermatitis, confocal microscopy, skin, rhesus monkey, Macaca mulatta

 JoVE Immunology and Infection

Assessing Hepatic Metabolic Changes During Progressive Colonization of Germ-free Mouse by 1H NMR Spectroscopy


JoVE 3642

1, 2

1School of Chemistry, Food and Pharmacy, The University of Reading, 2Department of Nutritional Sciences, The University of Reading

Date Published: December 15, 2011  |  doi:10.3791/3642  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Germ-free animal, colonization, NMR, HR MAS NMR, metabonomics

 JoVE Clinical and Translational Medicine

Longitudinal Evaluation of Mouse Hind Limb Bone Loss After Spinal Cord Injury using Novel, in vivo, Methodology


JoVE 3246

,

Department of Integrative Biology and Pharmacology, University of Texas Health Science Center at Houston

Date Published: December 7, 2011  |  doi:10.3791/3246  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, spinal cord injury, bone, osteoporosis, x-ray, femur, tibia, longitudinal

 JoVE Clinical and Translational Medicine

Modeling and Imaging 3-Dimensional Collective Cell Invasion


JoVE 3525

1, 2, 2

1Strathclyde Institute for Pharmacy and Biomedical Sciences, University of Strathclyde, 2The Beatson Institute for Cancer Research

Date Published: December 7, 2011  |  doi:10.3791/3525  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, cancer, cell invasion, imaging, retroviral labeling, RNAi, 3D, Matrix, Matrigel, ECM

 JoVE Clinical and Translational Medicine

Skeletal Muscle Gender Dimorphism from Proteomics


JoVE 3536

1, 2, 3, 4

1Center for Proteomics, Smith College, 2Department of Molecular Biophysics and Biochemistry, Yale University, 3Department of Chemistry, Smith College, 4Department of Biological Sciences and Center for Proteomics, Smith College

Date Published: December 14, 2011  |  doi:10.3791/3536  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, skeletal muscle, gender, 2-D gel electrophoresis, HPLC/MS, mouse

 JoVE Clinical and Translational Medicine

Method to Measure Tone of Axial and Proximal Muscle


JoVE 3677

1, 2, 1, 3

1Department of Biomedical Engineering, Oregon Health and Science University, 2UCL Institute of Neurology, Queen Square, 3Department of Neurology, Oregon Health and Science University

Date Published: December 14, 2011  |  doi:10.3791/3677  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Muscle Tone, Posture, Stiffness, Motor Control

 JoVE Clinical and Translational Medicine

Evaluation of Cancer Stem Cell Migration Using Compartmentalizing Microfluidic Devices and Live Cell Imaging


JoVE 3297

*1,2, *3, 3, 1,2,3, 3,4

1Department of Biomedical Engineering, University of Wisconsin-Madison, 2Materials Science Program, University of Wisconsin-Madison, 3Department of Neurological Surgery, University of Wisconsin-Madison, 4Carbone Comprehensive Cancer Center and Center for Stem Cell and Regenerative Medicine, University of Wisconsin-Madison

Date Published: December 23, 2011  |  doi:10.3791/3297  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, BTSC, Tumor, cancer stem cell, cell migration, microfluidics, Glioblastoma Multiforme, GBM, chemotaxis, amoeboid, mesenchymal, haptotaxis, PDMS

 JoVE Bioengineering

Planar and Three-Dimensional Printing of Conductive Inks


JoVE 3189

1, 1, 1, 1, 1, 1, 1,2, 1,3, 1

1Department of Materials Science and Engineering, University of Illinois at Urbana-Champaign, 2Center for Micro- and Nanotechnology, Lawrence Livermore National Laboratory, 3Presently at the Interdisciplinary Center for Wide Band-gap Semiconductors, University Of California Santa Barbara

Date Published: December 9, 2011  |  doi:10.3791/3189  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Direct-write assembly, silver ink, 3D printing, planar, three-dimensional, microelectrodes, flexible electronics, printed electronics

 JoVE Bioengineering

Specimen Preparation, Imaging, and Analysis Protocols for Knife-edge Scanning Microscopy


JoVE 3248

1, 2, 3, 1, 1, 1, 4, 1, 5

1Department of Computer Science and Engineering, Texas A&M University, 2Beckman Institute for Advanced Science and Technology, University of Illinois, 3Department of Electrical and Computer Engineering, Kettering University, 43Scan, 5Department of Veterinary Integrative Biosciences, Texas A&M University

Date Published: December 9, 2011  |  doi:10.3791/3248  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Physical sectioning, serial sectioning, light microscopy, brain imaging, microtome

 JoVE Bioengineering

Fluorescence detection methods for microfluidic droplet platforms


JoVE 3437

1, 1, 1, 1, 2, 1, 3

1Department of Chemistry, Imperial College London, 2Department of Biochemistry, Protein Chip Research Center, Chungbuk National University, 3Department of Chemistry and Applied Biosciences, Institute for Chemical and Bioengineering, ETH Zurich

Date Published: December 10, 2011  |  doi:10.3791/3437  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Droplet Microfluidics, Single Cell Assays, Single Molecule Assays, Fluorescence Spectroscopy, Fluorescence Lifetime Imaging

 JoVE Bioengineering

Soft Lithographic Functionalization and Patterning Oxide-free Silicon and Germanium


JoVE 3478

1, 1, 2, 3

1Department of Chemistry, Duke University, 2Hajim School of Engineering and Applied Sciences, University of Rochester, 3Department of Chemical Engineering, University of Rochester

Date Published: December 16, 2011  |  doi:10.3791/3478  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Soft lithography, microcontact printing, protein arrays, catalytic printing, oxide-free silicon

 JoVE Bioengineering

Visualizing Proteins and Macromolecular Complexes by Negative Stain EM: from Grid Preparation to Image Acquisition


JoVE 3227

1, 2, 2

1Graduate Group in Biophysics, University of California San Francisco, 2Department of Biochemistry and Biophysics, University of California San Francisco

Date Published: December 22, 2011  |  doi:10.3791/3227  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Electron Microscopy, EM, cryoEM, protein, negative stain, 3D structures

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