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Table of Contents

Table of Contents: Issue 70, December 2012

Jump to:  JoVE Editorial (1) JoVE Biology (13) JoVE Neuroscience (11) JoVE Immunology and Infection (10) JoVE Clinical and Translational Medicine (7) JoVE Bioengineering (3) JoVE Applied Physics (3)
 JoVE Biology

Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy

1Department of Biology, Howard Hughes Medical Institute, University of Utah


JoVE 3995

Date Published: December 3, 2012  |  doi:10.3791/3995  |  Section: JoVE Biology

 JoVE Biology

Use of Time Lapse Microscopy to Visualize Anoxia-induced Suspended Animation in C. elegans Embryos

1Department of Biological Sciences, University of North Texas


JoVE 4319

Date Published: December 3, 2012  |  doi:10.3791/4319  |  Section: JoVE Biology

 JoVE Biology

Spatial Multiobjective Optimization of Agricultural Conservation Practices using a SWAT Model and an Evolutionary Algorithm

1School of Environmental and Forest Sciences, University of Washington, 2Center for Agricultural and Rural Development, Department of Economics, Iowa State University, 3Department of Civil, Architectural, and Environmental Engineering, North Carolina A&T University, 4Iowa Geological and Water Survey


JoVE 4009

Date Published: December 9, 2012  |  doi:10.3791/4009  |  Section: JoVE Biology

 JoVE Biology

A Novel Bayesian Change-point Algorithm for Genome-wide Analysis of Diverse ChIPseq Data Types

1Department of Applied Mathematics & Statistics, Stony Brook University, 2Computational Biology and Bioinformatics, Cold Spring Harbor Laboratory, 3Department of Molecular and Cell Biology, University of Texas at Dallas


JoVE 4273

Date Published: December 10, 2012  |  doi:10.3791/4273  |  Section: JoVE Biology

 JoVE Biology

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization

1Department of Biochemistry and Molecular Biology, Knight Cancer Institute, Oregon Health & Science University


JoVE 4400

Date Published: December 10, 2012  |  doi:10.3791/4400  |  Section: JoVE Biology

 JoVE Biology

Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes

1Department of Flow and Image Cytometry, Roswell Park Cancer Institute, 2Flow Cytometry & Cell Sorting Resource Laboratory, University of Pennsylvania, 3SciGro, Inc., 4Department of Pathology and Laboratory Medicine, University of Pennsylvania


JoVE 4287

Date Published: December 13, 2012  |  doi:10.3791/4287  |  Section: JoVE Biology

 JoVE Biology

Monitoring Plasmid Replication in Live Mammalian Cells over Multiple Generations by Fluorescence Microscopy

1Department of Oncology, University of Wisconsin - Madison


JoVE 4305

Date Published: December 13, 2012  |  doi:10.3791/4305  |  Section: JoVE Biology

 JoVE Biology

The Green Monster Process for the Generation of Yeast Strains Carrying Multiple Gene Deletions

1Department of Synthetic Biology and Bioenergy, J. Craig Venter Institute, 2Department of Microbial and Environmental Genomics, J. Craig Venter Institute, 3Donnelly Centre & Department of Molecular Genetics, University of Toronto, 4Lunenfeld Research Institute, Mt Sinai Hospital


JoVE 4072

Date Published: December 15, 2012  |  doi:10.3791/4072  |  Section: JoVE Biology

 JoVE Biology

Glycan Profiling of Plant Cell Wall Polymers using Microarrays

1Australian Centre of Excellence in Plant Cell Walls, School of Botany, University of Melbourne, 2Plant Cell Biology Research Centre, School of Botany, University of Melbourne, 3CSIRO Plant Industry, Black Mountain Laboratories, 4Department of Plant Biology and Biotechnology, University of Copenhagen


JoVE 4238

Date Published: December 17, 2012  |  doi:10.3791/4238  |  Section: JoVE Biology

 JoVE Biology

Visualization of Endoplasmic Reticulum Localized mRNAs in Mammalian Cells

1Department of Biochemistry, University of Toronto


JoVE 50066

Date Published: December 17, 2012  |  doi:10.3791/50066  |  Section: JoVE Biology

 JoVE Biology

Cryosectioning Yeast Communities for Examining Fluorescence Patterns

1Division of Basic Sciences, Fred Hutchinson Cancer Research Center


JoVE 50101

Date Published: December 26, 2012  |  doi:10.3791/50101  |  Section: JoVE Biology

 JoVE Biology

Measurement of Leaf Hydraulic Conductance and Stomatal Conductance and Their Responses to Irradiance and Dehydration Using the Evaporative Flux Method (EFM)

1University of California, Los Angeles


JoVE 4179

Date Published: December 31, 2012  |  doi:10.3791/4179  |  Section: JoVE Biology

 JoVE Biology

Long-term, High-resolution Confocal Time Lapse Imaging of Arabidopsis Cotyledon Epidermis during Germination

1Department of Biology, University of Washington, 2Howard Hughes Medical Institute, University of Washington, 3PRESTO, Japan Science and Technology Agency


JoVE 4426

Date Published: December 31, 2012  |  doi:10.3791/4426  |  Section: JoVE Biology

 JoVE Neuroscience

Simultaneous Intracellular Recording of a Lumbar Motoneuron and the Force Produced by its Motor Unit in the Adult Mouse In vivo

1Department of Physiology, Northwestern University Feinberg School of Medicine


JoVE 4312

Date Published: December 5, 2012  |  doi:10.3791/4312  |  Section: JoVE Neuroscience

 JoVE Neuroscience

An In Vitro Preparation for Eliciting and Recording Feeding Motor Programs with Physiological Movements in Aplysia californica

1Department of Biology, Case Western Reserve University, 2Department of Neurosciences, Case Western Reserve University, 3Department of Biomedical Engineering, Case Western Reserve University


JoVE 4320

Date Published: December 5, 2012  |  doi:10.3791/4320  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Methods for Intravenous Self Administration in a Mouse Model

1Addictions Unit, McGill University Health Centre


JoVE 3739

Date Published: December 8, 2012  |  doi:10.3791/3739  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging

1Department of Neuroscience, Medical University of South Carolina


JoVE 50025

Date Published: December 12, 2012  |  doi:10.3791/50025  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Tactile Conditioning And Movement Analysis Of Antennal Sampling Strategies In Honey Bees (Apis mellifera L.)

1Biological Cybernetics, CITEC - Cognitive Interaction Technology - Center of Excellence, Bielefeld University


JoVE 50179

Date Published: December 12, 2012  |  doi:10.3791/50179  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Manual Drainage of the Zebrafish Embryonic Brain Ventricles

1Department of Biology, Whitehead Institute of Biomedical Research, Massachusetts Institute of Technology


JoVE 4243

Date Published: December 16, 2012  |  doi:10.3791/4243  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Simultaneous Electroencephalography, Real-time Measurement of Lactate Concentration and Optogenetic Manipulation of Neuronal Activity in the Rodent Cerebral Cortex

1Department of Veterinary & Comparative Anatomy, Pharmacology and Physiology, Sleep and Performance Research Center, WWAMI Medical Education Program, Washington State University


JoVE 4328

Date Published: December 19, 2012  |  doi:10.3791/4328  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Assaying DNA Damage in Hippocampal Neurons Using the Comet Assay

1Department of Radiation Oncology, University of Alabama-Birmingham, 2Department of Radiation Oncology, The Ohio State University Medical School, 3Department of Cell Biology, and Pharmacology and Toxicology, Comprehensive Cancer Center, University of Alabama at Birmingham School of Medicine, University of Alabama-Birmingham


JoVE 50049

Date Published: December 19, 2012  |  doi:10.3791/50049  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Progenitor-derived Oligodendrocyte Culture System from Human Fetal Brain

1Laboratory of Molecular Medicine and Neuroscience, National Institute of Neurological Disorders and Stroke, National Institutes of Health, 2Laboratory of Neurophysiology, National Institute of Neurological Disorders and Stroke, National Institutes of Health


JoVE 4274

Date Published: December 20, 2012  |  doi:10.3791/4274  |  Section: JoVE Neuroscience

 JoVE Neuroscience

A Molecular Readout of Long-term Olfactory Adaptation in C. elegans

1Department of Biological Sciences and Institute for Neuroscience, George Washington University, 2Fred Hutchinson Cancer Research Center, 3Department of Cell and Tissue Biology, University of California San Francisco


JoVE 4443

Date Published: December 22, 2012  |  doi:10.3791/4443  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Dissection, Culture, and Analysis of Xenopus laevis Embryonic Retinal Tissue

1Department of Biology, College of William and Mary


JoVE 4377

Date Published: December 23, 2012  |  doi:10.3791/4377  |  Section: JoVE Neuroscience

 JoVE Immunology and Infection

In Vivo Imaging Systems (IVIS) Detection of a Neuro-Invasive Encephalitic Virus

1Experimental Pathology, University of Texas Medical Branch


JoVE 4429

Date Published: December 2, 2012  |  doi:10.3791/4429  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

A Quantitative Evaluation of Cell Migration by the Phagokinetic Track Motility Assay

1Department of Microbiology and Immunology, Louisiana State University Health Sciences Center, 2Center for Molecular and Tumor Virology, Louisiana State University Health Sciences Center, 3Department of Microbiology and Immunology, SUNY Upstate Medical University, 4Feist-Weiller Cancer Center, Louisiana State University Health Sciences Center


JoVE 4165

Date Published: December 4, 2012  |  doi:10.3791/4165  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Quantitative Measurement of the Immune Response and Sleep in Drosophila

1Center for Sleep and Circadian Neurobiology, University of Pennsylvania Perelman School of Medicine


JoVE 4355

Date Published: December 4, 2012  |  doi:10.3791/4355  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Rearing and Injection of Manduca sexta Larvae to Assess Bacterial Virulence

1Department of Bacteriology, University of Wisconsin-Madison


JoVE 4295

Date Published: December 11, 2012  |  doi:10.3791/4295  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

The Insect Galleria mellonella as a Powerful Infection Model to Investigate Bacterial Pathogenesis

1INRA, Micalis UMR1319, France


JoVE 4392

Date Published: December 11, 2012  |  doi:10.3791/4392  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Using the BLT Humanized Mouse as a Stem Cell based Gene Therapy Tumor Model

1Department of Medicine, Division of Hematology-Oncology, David Geffen School of Medicine at UCLA, 2UCLA AIDS Institute, 3Eli & Edythe Broad Center of Regenerative Medicine and Stem Cell Research at UCLA, 4Department of Medical and Molecular Pharmacology, David Geffen School of Medicine at UCLA, 5Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine at UCLA


JoVE 4181

Date Published: December 18, 2012  |  doi:10.3791/4181  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Recurrent Herpetic Stromal Keratitis in Mice, a Model for Studying Human HSK

1Department of Ophthalmology, Saint Louis University


JoVE 4276

Date Published: December 18, 2012  |  doi:10.3791/4276  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies

1Research Group Immune Regulation, Helmholtz Centre for Infection Research, 2Research Group Infection Immunology, Institute of Medical Microbiology, Otto-von-Guericke University, 3Department of Experimental Immunology, Helmholtz Centre for Infection Research


JoVE 4322

Date Published: December 26, 2012  |  doi:10.3791/4322  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Artificial Antigen Presenting Cell (aAPC) Mediated Activation and Expansion of Natural Killer T Cells

1Department of Microbiology and Immunology, University of Maryland


JoVE 4333

Date Published: December 29, 2012  |  doi:10.3791/4333  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

A Parasite Rescue and Transformation Assay for Antileishmanial Screening Against Intracellular Leishmania donovani Amastigotes in THP1 Human Acute Monocytic Leukemia Cell Line

1National Center for Natural Products Research, School of Pharmacy, University of Mississippi, 2Department of Pharmacology, University of Mississippi


JoVE 4054

Date Published: December 30, 2012  |  doi:10.3791/4054  |  Section: JoVE Immunology and Infection

 JoVE Clinical and Translational Medicine

Preparation and Pathogen Inactivation of Double Dose Buffy Coat Platelet Products using the INTERCEPT Blood System

1Department of Laboratory Medicine, Section for Transfusion Medicine, Örebro University Hospital


JoVE 4414

Date Published: December 7, 2012  |  doi:10.3791/4414  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Surgical Technique for Spinal Cord Delivery of Therapies: Demonstration of Procedure in Gottingen Minipigs

1Department of Neurosurgery, Emory University, 2Department of Neuroscience, Medical University of South Carolina, 3Division of Neurosurgery, University of Alabama, Birmingham, 4Department of Biomedical Engineering, Georgia Institute of Technology, 5Department of Biomedical Engineering, Emory University


JoVE 4371

Date Published: December 7, 2012  |  doi:10.3791/4371  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Portable Intermodal Preferential Looking (IPL): Investigating Language Comprehension in Typically Developing Toddlers and Young Children with Autism

1Department of Psychology, University of Connecticut


JoVE 4331

Date Published: December 14, 2012  |  doi:10.3791/4331  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

The Measurement and Treatment of Suppression in Amblyopia

1Department of Optometry and Vision Science, University of Auckland, 2Department of Ophthalmology, McGill University, 3Centre for Intelligent Machines, McGill University


JoVE 3927

Date Published: December 14, 2012  |  doi:10.3791/3927  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Intraoperative Detection of Subtle Endometriosis: A Novel Paradigm for Detection and Treatment of Pelvic Pain Associated with the Loss of Peritoneal Integrity

1Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, Greenville Hospital System, 2Department of Pathology, Duke University Health System, 3Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, Duke University


JoVE 4313

Date Published: December 21, 2012  |  doi:10.3791/4313  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Quantitative Analysis of Chromatin Proteomes in Disease

1Department of Anesthesiology, David Geffen School of Medicine at UCLA, 2Department of Medicine, David Geffen School of Medicine at UCLA, 3Department of Physiology, David Geffen School of Medicine at UCLA, 4Department of Internal Medicine, Nora Eccles Harrison Cardiovascular Research and Training Institute, University of Utah


JoVE 4294

Date Published: December 28, 2012  |  doi:10.3791/4294  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

A Research Method For Detecting Transient Myocardial Ischemia In Patients With Suspected Acute Coronary Syndrome Using Continuous ST-segment Analysis

1Orvis School of Nursing, University of Nevada, Reno, 2The State University of New York at Buffalo, St. Joseph's Medical Center, 3Strong Memorial Hospital, University of Rochester Medical Center


JoVE 50124

Date Published: December 28, 2012  |  doi:10.3791/50124  |  Section: JoVE Clinical and Translational Medicine

 JoVE Bioengineering

Tissue Engineering of the Intestine in a Murine Model

1Children's Hospital Los Angeles, Division of Pediatric Surgery, Saban Research Institute, Keck School of Medicine of the University of Southern California


JoVE 4279

Date Published: December 1, 2012  |  doi:10.3791/4279  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Procedure for Decellularization of Porcine Heart by Retrograde Coronary Perfusion

1McGowan Institute for Regenerative Medicine, 2Department of Bioengineering, University of Pittsburgh, 3Department of Cardiothoracic Surgery, Children's Hospital of Pittsburgh of UPMC, 4Department of Surgery, University of Pittsburgh


JoVE 50059

Date Published: December 6, 2012  |  doi:10.3791/50059  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Generation and Recovery of β-cell Spheroids From Step-growth PEG-peptide Hydrogels

1Department of Biomedical Engineering, Purdue School of Engineering and Technology, Indiana University - Purdue University at Indianapolis


JoVE 50081

Date Published: December 6, 2012  |  doi:10.3791/50081  |  Section: JoVE Bioengineering

 JoVE Applied Physics

Revealing Dynamic Processes of Materials in Liquids Using Liquid Cell Transmission Electron Microscopy

1Materials Sciences Division, Lawrence Berkeley National Laboratory


JoVE 50122

Date Published: December 20, 2012  |  doi:10.3791/50122  |  Section: JoVE Applied Physics

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