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The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.

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Table of Contents

Table of Contents: Issue 70, December 2012

Jump to:  JoVE Editorial (1) JoVE General (13) JoVE Neuroscience (11) JoVE Immunology and Infection (10) JoVE Clinical and Translational Medicine (7) JoVE Bioengineering (3) JoVE Applied Physics (3)
 JoVE General

Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy


JoVE 3995

, , , , ,

Department of Biology, Howard Hughes Medical Institute, University of Utah

Date Published: December 3, 2012  |  doi:10.3791/3995  |  Section: JoVE General

Keywords: Molecular Biology, Cellular Biology, Genetics, Proteomics, Proteins, Protein localization, super-resolution fluorescence microscopy, fluorescence, electron microscopy, nano-fEM, EM, SEM, electron micrograph, imaging

 JoVE General

Use of Time Lapse Microscopy to Visualize Anoxia-induced Suspended Animation in C. elegans Embryos


JoVE 4319

, ,

Department of Biological Sciences, University of North Texas

Date Published: December 3, 2012  |  doi:10.3791/4319  |  Section: JoVE General

Keywords: Developmental Biology, Cellular Biology, Molecular Biology, Anatomy, Physiology, C. elegans, Caenorhabdits elegans, anoxia, suspended animation, in vivo imaging, microscopy, oxygen deprivation, cell cycle, animal model

 JoVE General

Spatial Multiobjective Optimization of Agricultural Conservation Practices using a SWAT Model and an Evolutionary Algorithm


JoVE 4009

1, 2, 2, 2, 3, 4, 4, 2

1School of Environmental and Forest Sciences, University of Washington, 2Center for Agricultural and Rural Development, Department of Economics, Iowa State University, 3Department of Civil, Architectural, and Environmental Engineering, North Carolina A&T University, 4Iowa Geological and Water Survey

Date Published: December 9, 2012  |  doi:10.3791/4009  |  Section: JoVE General

Keywords: Environmental Sciences, Plant Biology, Civil Engineering, Forest Sciences, Water quality, multiobjective optimization, evolutionary algorithms, cost efficiency, agriculture, development

 JoVE General

A Novel Bayesian Change-point Algorithm for Genome-wide Analysis of Diverse ChIPseq Data Types


JoVE 4273

1, 1,2, 1,2, 2,3

1Department of Applied Mathematics & Statistics, Stony Brook University, 2Computational Biology and Bioinformatics, Cold Spring Harbor Laboratory, 3Department of Molecular and Cell Biology, University of Texas at Dallas

Date Published: December 10, 2012  |  doi:10.3791/4273  |  Section: JoVE General

Keywords: Genetics, Bioinformatics, Genomics, Molecular Biology, Cellular Biology, Immunology, Chromatin immunoprecipitation, ChIP-Seq, histone modifications, segmentation, Bayesian, Hidden Markov Models, epigenetics

 JoVE General

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization


JoVE 4400

,

Department of Biochemistry and Molecular Biology, Knight Cancer Institute, Oregon Health & Science University

Date Published: December 10, 2012  |  doi:10.3791/4400  |  Section: JoVE General

Keywords: Genetics, Biochemistry, Molecular Biology, Cellular Biology, Chromosome replication timing, fluorescent in situ hybridization, FISH, BrdU, cytogenetics, chromosome rearrangements, fluorescence microscopy

 JoVE General

Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes


JoVE 4287

1, 1, 2, 3, 4, 1

1Department of Flow and Image Cytometry, Roswell Park Cancer Institute, 2Flow Cytometry & Cell Sorting Resource Laboratory, University of Pennsylvania, 3SciGro, Inc., 4Department of Pathology and Laboratory Medicine, University of Pennsylvania

Date Published: December 13, 2012  |  doi:10.3791/4287  |  Section: JoVE General

Keywords: Cellular Biology, Molecular Biology, Cell tracking, PKH26, CFSE, membrane dyes, dye dilution, proliferation modeling, lymphocytes

 JoVE General

Monitoring Plasmid Replication in Live Mammalian Cells over Multiple Generations by Fluorescence Microscopy


JoVE 4305

, ,

Department of Oncology, University of Wisconsin - Madison

Date Published: December 13, 2012  |  doi:10.3791/4305  |  Section: JoVE General

Keywords: Genetics, Molecular Biology, Cellular Biology, Genomics, DNA synthesis, DNA partitioning, plasmids, lac operator, lac repressor, mammalian cells, fluorescence microscopy

 JoVE General

The Green Monster Process for the Generation of Yeast Strains Carrying Multiple Gene Deletions


JoVE 4072

1, 1, 2, 3, 2, 3,4

1Department of Synthetic Biology and Bioenergy, J. Craig Venter Institute, 2Department of Microbial and Environmental Genomics, J. Craig Venter Institute, 3Donnelly Centre & Department of Molecular Genetics, University of Toronto, 4Lunenfeld Research Institute, Mt Sinai Hospital

Date Published: December 15, 2012  |  doi:10.3791/4072  |  Section: JoVE General

Keywords: Microbiology, Genetics, Synthetic Biology, Environmental Genomics, Genomics, Bioengineering, Biomedical Engineering, Cellular Biology, Multi-site genomic engineering, genetic interaction, green fluorescent protein, GFP, flow cytometry, Saccharomyces cerevisiae, yeast, Green Monster

 JoVE General

Glycan Profiling of Plant Cell Wall Polymers using Microarrays


JoVE 4238

1,2, 3, 1, 1, 4, 1,2

1Australian Centre of Excellence in Plant Cell Walls, School of Botany, University of Melbourne, 2Plant Cell Biology Research Centre, School of Botany, University of Melbourne, 3CSIRO Plant Industry, Black Mountain Laboratories, 4Department of Plant Biology and Biotechnology, University of Copenhagen

Date Published: December 17, 2012  |  doi:10.3791/4238  |  Section: JoVE General

Keywords: Plant Biology, Molecular Biology, Cellular Biology, Genetics, Genomics, Proteomics, Proteins, Cell Walls, Polysaccharides, Monoclonal Antibodies, Microarrays, CoMPP, glycans, Arabidopsis, tissue collection

 JoVE General

Visualization of Endoplasmic Reticulum Localized mRNAs in Mammalian Cells


JoVE 50066

,

Department of Biochemistry, University of Toronto

Date Published: December 17, 2012  |  doi:10.3791/50066  |  Section: JoVE General

Keywords: Cellular Biology, Biochemistry, Genetics, Molecular Biology, Genomics, mRNA localization, RNA, digitonin extraction, cell fractionation, endoplasmic reticulum, secretion, microscopy, imaging, fluorescent in situ hybridization, FISH, cell biology

 JoVE General

Cryosectioning Yeast Communities for Examining Fluorescence Patterns


JoVE 50101

,

Division of Basic Sciences, Fred Hutchinson Cancer Research Center

Date Published: December 26, 2012  |  doi:10.3791/50101  |  Section: JoVE General

Keywords: Microbiology, Molecular Biology, Cellular Biology, Basic Protocols, Yeasts, Saccharomyces cerevisiae, Clinical Laboratory Techniques, Cytological Techniques, Environmental Microbiology, Investigative Techniques, Life Sciences, cryosectioning, sectioning, cryotome, fixing, microbial community, yeast colonies, Saccharomyces cerevisiae, community interactions

 JoVE General

Measurement of Leaf Hydraulic Conductance and Stomatal Conductance and Their Responses to Irradiance and Dehydration Using the Evaporative Flux Method (EFM)


JoVE 4179

,

University of California, Los Angeles

Date Published: December 31, 2012  |  doi:10.3791/4179  |  Section: JoVE General

Keywords: Plant Biology, Molecular Biology, Physiology, Ecology, Biology, Botany, Leaf traits, hydraulics, stomata, transpiration, xylem, conductance, leaf hydraulic conductance, resistance, evaporative flux method, whole plant

 JoVE General

Long-term, High-resolution Confocal Time Lapse Imaging of Arabidopsis Cotyledon Epidermis during Germination


JoVE 4426

1, 1,2,3

1Department of Biology, University of Washington, 2Howard Hughes Medical Institute, University of Washington, 3PRESTO, Japan Science and Technology Agency

Date Published: December 31, 2012  |  doi:10.3791/4426  |  Section: JoVE General

Keywords: Plant Biology, Molecular Biology, Developmental Biology, Cellular Biology, Botany, plant, live imaging, epidermis, stomata, confocal, time lapse, Arabidopsis, cotyledon

 JoVE Neuroscience

Simultaneous Intracellular Recording of a Lumbar Motoneuron and the Force Produced by its Motor Unit in the Adult Mouse In vivo


JoVE 4312

,

Department of Physiology, Northwestern University Feinberg School of Medicine

Date Published: December 5, 2012  |  doi:10.3791/4312  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Physiology, Biophysics, Anatomy, Medicine, Motor System, Spinal Cord, Intracellular Recordings, Motoneurons, EMG, Force, lumbar, neuron, brain, mouse, animal model

 JoVE Neuroscience

An In Vitro Preparation for Eliciting and Recording Feeding Motor Programs with Physiological Movements in Aplysia californica


JoVE 4320

1, 1, 1,2,3

1Department of Biology, Case Western Reserve University, 2Department of Neurosciences, Case Western Reserve University, 3Department of Biomedical Engineering, Case Western Reserve University

Date Published: December 5, 2012  |  doi:10.3791/4320  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Physiology, Biomedical Engineering, Anatomy, Marine Biology, Aplysia, Aplysia californica, California sea slug, invertebrate, feeding, neurobiology, buccal mass, semi-intact preparation, extracellular electrodes, extracellular recording, neurons, animal model

 JoVE Neuroscience

Methods for Intravenous Self Administration in a Mouse Model


JoVE 3739

, ,

Addictions Unit, McGill University Health Centre

Date Published: December 8, 2012  |  doi:10.3791/3739  |  Section: JoVE Neuroscience

Keywords: Medicine, Neuroscience, Pharmacology, Behavior, Anatomy, Physiology, Surgery, Intravenous self-administration, IVSA, catheterization, indwelling catheters, drug abuse, addiction, operant training, mouse, animal model

 JoVE Neuroscience

Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging


JoVE 50025

, , , , ,

Department of Neuroscience, Medical University of South Carolina

Date Published: December 12, 2012  |  doi:10.3791/50025  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Molecular Biology, Cellular Biology, Anatomy, Physiology, Two-photon imaging, non-rodent, cortical maps, functional architecture, orientation pinwheel singularity, optical imaging, calcium-sensitive dye, bulk loading, single-cell electroporation

 JoVE Neuroscience

Tactile Conditioning And Movement Analysis Of Antennal Sampling Strategies In Honey Bees (Apis mellifera L.)


JoVE 50179

, , ,

Biological Cybernetics, CITEC - Cognitive Interaction Technology - Center of Excellence, Bielefeld University

Date Published: December 12, 2012  |  doi:10.3791/50179  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Physiology, Anatomy, Entomology, Behavior, Sensilla, Bees, behavioral sciences, Sense Organs, Honey bee, Apis mellifera L., Insect antenna, Tactile sampling, conditioning, Proboscis extension response, Motion capture

 JoVE Neuroscience

Manual Drainage of the Zebrafish Embryonic Brain Ventricles


JoVE 4243

,

Department of Biology, Whitehead Institute of Biomedical Research, Massachusetts Institute of Technology

Date Published: December 16, 2012  |  doi:10.3791/4243  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Developmental Biology, Medicine, Anatomy, Physiology, Zebrafish, Danio rerio, eCSF, neuroepithelium, brain ventricular system, brain, microsurgery, animal model

 JoVE Neuroscience

Simultaneous Electroencephalography, Real-time Measurement of Lactate Concentration and Optogenetic Manipulation of Neuronal Activity in the Rodent Cerebral Cortex


JoVE 4328

, , ,

Department of Veterinary & Comparative Anatomy, Pharmacology and Physiology, Sleep and Performance Research Center, WWAMI Medical Education Program, Washington State University

Date Published: December 19, 2012  |  doi:10.3791/4328  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Physiology, Anatomy, Medicine, Pharmacology, Surgery, Sleep, rapid eye movement, glucose, glycolysis, pyramidal neurons, channelrhodopsin, optogenetics, optogenetic stimulation, electroencephalogram, EEG, EMG, brain, animal model

 JoVE Neuroscience

Assaying DNA Damage in Hippocampal Neurons Using the Comet Assay


JoVE 50049

1, 2, 1,3

1Department of Radiation Oncology, University of Alabama-Birmingham, 2Department of Radiation Oncology, The Ohio State University Medical School, 3Department of Cell Biology, and Pharmacology and Toxicology, Comprehensive Cancer Center, University of Alabama at Birmingham School of Medicine, University of Alabama-Birmingham

Date Published: December 19, 2012  |  doi:10.3791/50049  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Genetics, Cellular Biology, Molecular Biology, Medicine, Cancer Biology, Anatomy, Physiology, DNA, DNA damage, double strand break, single strand break, repair, neurons, comet assay, cell culture

 JoVE Neuroscience

Progenitor-derived Oligodendrocyte Culture System from Human Fetal Brain


JoVE 4274

1, 2, 1, 1, 1, 1

1Laboratory of Molecular Medicine and Neuroscience, National Institute of Neurological Disorders and Stroke, National Institutes of Health, 2Laboratory of Neurophysiology, National Institute of Neurological Disorders and Stroke, National Institutes of Health

Date Published: December 20, 2012  |  doi:10.3791/4274  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Developmental Biology, Medicine, Stem Cell Biology, Molecular Biology, Cellular Biology, Physiology, lineage characterization, neural progenitors, differentiation, cell culture model

 JoVE Neuroscience

A Molecular Readout of Long-term Olfactory Adaptation in C. elegans


JoVE 4443

1, 2, 3, 1

1Department of Biological Sciences and Institute for Neuroscience, George Washington University, 2Fred Hutchinson Cancer Research Center, 3Department of Cell and Tissue Biology, University of California San Francisco

Date Published: December 22, 2012  |  doi:10.3791/4443  |  Section: JoVE Neuroscience

Keywords: Developmental Biology, Neuroscience, Molecular Biology, Cellular Biology, Olfactory adaptation, C. elegans, EGL-4, nuclear translocation, olfaction, animal model

 JoVE Neuroscience

Dissection, Culture, and Analysis of Xenopus laevis Embryonic Retinal Tissue


JoVE 4377

*, *, , , ,

Department of Biology, College of William and Mary

Date Published: December 23, 2012  |  doi:10.3791/4377  |  Section: JoVE Neuroscience

Keywords: Developmental Biology, Neuroscience, Cellular Biology, Surgery, Anatomy, Physiology, Ophthalmology, retina, primary cell culture, dissection, confocal microscopy, calcium imaging, fluorescent in situ hybridization, FISH, Xenopus laevis, animal model

 JoVE Immunology and Infection

In Vivo Imaging Systems (IVIS) Detection of a Neuro-Invasive Encephalitic Virus


JoVE 4429

*, *, , , , , ,

Experimental Pathology, University of Texas Medical Branch

Date Published: December 2, 2012  |  doi:10.3791/4429  |  Section: JoVE Immunology and Infection

Keywords: Virology, Immunology, Medicine, Neuroscience, Molecular Biology, Pathology, IVIS, in vivo modeling, VEE, CNS, Neuroinvasion, Hume’s 3Rs, Encephalitis, bioluminescence, luciferase, virus

 JoVE Immunology and Infection

A Quantitative Evaluation of Cell Migration by the Phagokinetic Track Motility Assay


JoVE 4165

1,2, 3, 1,2, 1,2, 1,2,4

1Department of Microbiology and Immunology, Louisiana State University Health Sciences Center, 2Center for Molecular and Tumor Virology, Louisiana State University Health Sciences Center, 3Department of Microbiology and Immunology, SUNY Upstate Medical University, 4Feist-Weiller Cancer Center, Louisiana State University Health Sciences Center

Date Published: December 4, 2012  |  doi:10.3791/4165  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Microbiology, Cellular Biology, Molecular Biology, gold nanoparticles, coverslips, cell migration, quantitative cell movement, microscopy, motility, assay

 JoVE Immunology and Infection

Quantitative Measurement of the Immune Response and Sleep in Drosophila


JoVE 4355

, ,

Center for Sleep and Circadian Neurobiology, University of Pennsylvania Perelman School of Medicine

Date Published: December 4, 2012  |  doi:10.3791/4355  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Neuroscience, Medicine, Physiology, Pathology, Microbiology, immune response, sleep, Drosophila, infection, bacteria, luciferase reporter assay, animal model

 JoVE Immunology and Infection

Rearing and Injection of Manduca sexta Larvae to Assess Bacterial Virulence


JoVE 4295

,

Department of Bacteriology, University of Wisconsin-Madison

Date Published: December 11, 2012  |  doi:10.3791/4295  |  Section: JoVE Immunology and Infection

Keywords: Infection, Microbiology, Immunology, Bacteriology, Entomology, Bacteria, injection, pathogenesis, insect larvae, instar, Manduca sexta, tobacco hornworm, animal model, host pathogen interactions

 JoVE Immunology and Infection

The Insect Galleria mellonella as a Powerful Infection Model to Investigate Bacterial Pathogenesis


JoVE 4392

, ,

INRA, Micalis UMR1319, France

Date Published: December 11, 2012  |  doi:10.3791/4392  |  Section: JoVE Immunology and Infection

Keywords: Infection, Microbiology, Immunology, Molecular Biology, Bacteriology, Entomology, Bacteria, Galleria mellonella, greater wax moth, insect larvae, intra haemocoelic injection, ingestion, animal model, host pathogen interactions

 JoVE Immunology and Infection

Using the BLT Humanized Mouse as a Stem Cell based Gene Therapy Tumor Model


JoVE 4181

1,2,3, 1,2, 1,2, 1,2, 4, 4, 1,2,3, 1,2,5

1Department of Medicine, Division of Hematology-Oncology, David Geffen School of Medicine at UCLA, 2UCLA AIDS Institute, 3Eli & Edythe Broad Center of Regenerative Medicine and Stem Cell Research at UCLA, 4Department of Medical and Molecular Pharmacology, David Geffen School of Medicine at UCLA, 5Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine at UCLA

Date Published: December 18, 2012  |  doi:10.3791/4181  |  Section: JoVE Immunology and Infection

Keywords: Cancer Biology, Stem Cell Biology, Immunology, Biomedical Engineering, Medicine, Bioengineering, Genetics, Oncology, Humanized mice, stem cell transplantation, stem cells, in vivo animal imaging, T cells, cancer, animal model

 JoVE Immunology and Infection

Recurrent Herpetic Stromal Keratitis in Mice, a Model for Studying Human HSK


JoVE 4276

, , , , ,

Department of Ophthalmology, Saint Louis University

Date Published: December 18, 2012  |  doi:10.3791/4276  |  Section: JoVE Immunology and Infection

Keywords: Infection, Immunology, Virology, Medicine, Infectious Diseases, Ophthalmology, Herpes, herpetic stromal keratitis, HSK, keratitis, pathogenesis, clinical evaluation, virus, eye, mouse, animal model

 JoVE Immunology and Infection

Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies


JoVE 4322

1,2, 1,2, 3, 2, 1,2, 1

1Research Group Immune Regulation, Helmholtz Centre for Infection Research, 2Research Group Infection Immunology, Institute of Medical Microbiology, Otto-von-Guericke University, 3Department of Experimental Immunology, Helmholtz Centre for Infection Research

Date Published: December 26, 2012  |  doi:10.3791/4322  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Cellular Biology, Molecular Biology, Infection, Infectious Diseases, Microbiology, alveolar type II epithelial cells, mouse, respiratory tract, lung, cell sorting, flow cytometry, influenza, autoimmunity

 JoVE Immunology and Infection

Artificial Antigen Presenting Cell (aAPC) Mediated Activation and Expansion of Natural Killer T Cells


JoVE 4333

*, *,

Department of Microbiology and Immunology, University of Maryland

Date Published: December 29, 2012  |  doi:10.3791/4333  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Medicine, Molecular Biology, Cellular Biology, Microbiology, Cancer Biology, Natural killer T cells, in vitro expansion, cancer immunology, artificial antigen presenting cells, adoptive transfer

 JoVE Immunology and Infection

A Parasite Rescue and Transformation Assay for Antileishmanial Screening Against Intracellular Leishmania donovani Amastigotes in THP1 Human Acute Monocytic Leukemia Cell Line


JoVE 4054

1, 1, 1,2, 1,2

1National Center for Natural Products Research, School of Pharmacy, University of Mississippi, 2Department of Pharmacology, University of Mississippi

Date Published: December 30, 2012  |  doi:10.3791/4054  |  Section: JoVE Immunology and Infection

Keywords: Infection, Immunology, Infectious Diseases, Molecular Biology, Cellular Biology, Pharmacology, Leishmania donovani, Visceral Leishmaniasis, THP1 cells, Drug Screening, Amastigotes, Antileishmanial drug assay

 JoVE Clinical and Translational Medicine

Preparation and Pathogen Inactivation of Double Dose Buffy Coat Platelet Products using the INTERCEPT Blood System


JoVE 4414

,

Department of Laboratory Medicine, Section for Transfusion Medicine, Örebro University Hospital

Date Published: December 7, 2012  |  doi:10.3791/4414  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Immunology, Hematology, Infectious Disease, Pathology, pathogen inactivation, pathogen reduction, double-dose platelets, INTERCEPT Blood System, amotosalen, UVA, platelet, blood processing, buffy coat, IBS, transfusion

 JoVE Clinical and Translational Medicine

Surgical Technique for Spinal Cord Delivery of Therapies: Demonstration of Procedure in Gottingen Minipigs


JoVE 4371

1, 1, 1, 1, 2, 1, 1, 3, 1, 1,4,5, 1

1Department of Neurosurgery, Emory University, 2Department of Neuroscience, Medical University of South Carolina, 3Division of Neurosurgery, University of Alabama, Birmingham, 4Department of Biomedical Engineering, Georgia Institute of Technology, 5Department of Biomedical Engineering, Emory University

Date Published: December 7, 2012  |  doi:10.3791/4371  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Neuroscience, Neurobiology, Anatomy, Physiology, Surgery, accuracy, delivery, safety, spinal cord, CNS, target, therapy, transplantation, swine, animal model

 JoVE Clinical and Translational Medicine

Portable Intermodal Preferential Looking (IPL): Investigating Language Comprehension in Typically Developing Toddlers and Young Children with Autism


JoVE 4331

,

Department of Psychology, University of Connecticut

Date Published: December 14, 2012  |  doi:10.3791/4331  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Neuroscience, Psychology, Behavior, Intermodal preferential looking, language comprehension, children with autism, child development, autism

 JoVE Clinical and Translational Medicine

The Measurement and Treatment of Suppression in Amblyopia


JoVE 3927

1, 2, 3, 3, 1

1Department of Optometry and Vision Science, University of Auckland, 2Department of Ophthalmology, McGill University, 3Centre for Intelligent Machines, McGill University

Date Published: December 14, 2012  |  doi:10.3791/3927  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Ophthalmology, Neuroscience, Anatomy, Physiology, Amblyopia, suppression, visual cortex, binocular vision, plasticity, strabismus, anisometropia

 JoVE Clinical and Translational Medicine

Intraoperative Detection of Subtle Endometriosis: A Novel Paradigm for Detection and Treatment of Pelvic Pain Associated with the Loss of Peritoneal Integrity


JoVE 4313

1, 1, 2, 3

1Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, Greenville Hospital System, 2Department of Pathology, Duke University Health System, 3Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, Duke University

Date Published: December 21, 2012  |  doi:10.3791/4313  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Anatomy, Physiology, Endocrinology, Obstetrics, Gynecology, Surgery, endometriosis, pelvic pain, dysmenorrhea, diagnostics, laparoscopy, peritoneum, scanning electron microscopy, SEM

 JoVE Clinical and Translational Medicine

Quantitative Analysis of Chromatin Proteomes in Disease


JoVE 4294

1, 1, 1, 1, 1,2,3, 1,4

1Department of Anesthesiology, David Geffen School of Medicine at UCLA, 2Department of Medicine, David Geffen School of Medicine at UCLA, 3Department of Physiology, David Geffen School of Medicine at UCLA, 4Department of Internal Medicine, Nora Eccles Harrison Cardiovascular Research and Training Institute, University of Utah

Date Published: December 28, 2012  |  doi:10.3791/4294  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Molecular Biology, Immunology, Genetics, Genomics, Physiology, Protein, DNA, Chromatin, cardiovascular disease, proteomics, mass spectrometry

 JoVE Clinical and Translational Medicine

A Research Method For Detecting Transient Myocardial Ischemia In Patients With Suspected Acute Coronary Syndrome Using Continuous ST-segment Analysis


JoVE 50124

1, 2, 1, 3

1Orvis School of Nursing, University of Nevada, Reno, 2The State University of New York at Buffalo, St. Joseph's Medical Center, 3Strong Memorial Hospital, University of Rochester Medical Center

Date Published: December 28, 2012  |  doi:10.3791/50124  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Anatomy, Physiology, Cardiology, Myocardial Ischemia, Cardiovascular Diseases, Health Occupations, Health Care, transient myocardial ischemia, Acute Coronary Syndrome, electrocardiogram, ST-segment monitoring, Holter monitoring, research methodology

 JoVE Bioengineering

Tissue Engineering of the Intestine in a Murine Model


JoVE 4279

, , , , , , ,

Children's Hospital Los Angeles, Division of Pediatric Surgery, Saban Research Institute, Keck School of Medicine of the University of Southern California

Date Published: December 1, 2012  |  doi:10.3791/4279  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Tissue Engineering, Biomedical Engineering, Medicine, Anatomy, Physiology, small intestine, pediatric surgery, short bowel syndrome, animal model, mouse

 JoVE Bioengineering

Procedure for Decellularization of Porcine Heart by Retrograde Coronary Perfusion


JoVE 50059

1,2, 1,3, 1,2,3,4

1McGowan Institute for Regenerative Medicine, 2Department of Bioengineering, University of Pittsburgh, 3Department of Cardiothoracic Surgery, Children's Hospital of Pittsburgh of UPMC, 4Department of Surgery, University of Pittsburgh

Date Published: December 6, 2012  |  doi:10.3791/50059  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Tissue Engineering, Biomedical Engineering, Cellular Biology, Medicine, Cardiology, Extracellular matrix, decellularization, animal model, porcine, cardiac, heart tissue

 JoVE Bioengineering

Generation and Recovery of β-cell Spheroids From Step-growth PEG-peptide Hydrogels


JoVE 50081

,

Department of Biomedical Engineering, Purdue School of Engineering and Technology, Indiana University - Purdue University at Indianapolis

Date Published: December 6, 2012  |  doi:10.3791/50081  |  Section: JoVE Bioengineering

Keywords: Biomedical Engineering, Bioengineering, Tissue Engineering, Cellular Biology, Molecular Biology, Biomaterials, beta cells, β-cell, PEG, PEG-peptide hydrogels, hydrogel, MIN6, poylmers, peptides, spheroids, pancreas

 JoVE Applied Physics

Revealing Dynamic Processes of Materials in Liquids Using Liquid Cell Transmission Electron Microscopy


JoVE 50122

, ,

Materials Sciences Division, Lawrence Berkeley National Laboratory

Date Published: December 20, 2012  |  doi:10.3791/50122  |  Section: JoVE Applied Physics

Keywords: Materials Science, Chemical Engineering, Chemistry, Physics, Engineering, Life sciences, Liquid cell, Transmission Electron Microscopy, TEM, In situ TEM, Single nanoparticle trajectory, dynamic imaging, nanocrystals

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