Table of Contents: Issue 7, August/September 2007

JoVE 7th Issue

JoVE 274

Date Published: August 30, 2007  |  doi:10.3791/274  |  Section: JoVE Editorial

Keywords: tissue engineering

Investigating the Immunological Mechanisms Underlying Organ Transplant Rejection

JoVE 256

Sang Mo Kang

Department of Surgery, University of California, San Francisco - UCSF

Date Published: August 20, 2007  |  doi:10.3791/256  |  Section: JoVE General

Keywords: Immunology, Heterotopic Heart Transplant, Small Bowel Transplant, Transplant Rejection, T regs, Diabetes, Autoimmune Disease, Translational Research

Regulatory T cells: Therapeutic Potential for Treating Transplant Rejection and Type I Diabetes

JoVE 257

Jeffry A. Bluestone

Diabetes Center, University of California, San Francisco - UCSF

Date Published: August 20, 2007  |  doi:10.3791/257  |  Section: JoVE General

Keywords: Immunology, Pancreatic Islets, Cell Culture, Diabetes, Ficoll Gradient, Translational Research

Small Bowel Transplantation In Mice

JoVE 258

Fengchun Liu, Sang-Mo Kang

Department of Surgery, University of California, San Francisco - UCSF

Date Published: August 20, 2007  |  doi:10.3791/258  |  Section: JoVE General

Keywords: Immunology, Transplantation, Transplant Rejection, Small Bowel

Patterning Cells on Optically Transparent Indium Tin Oxide Electrodes

JoVE 259

Sunny Shah, Alexander Revzin

Department of Biomedical Engineering, University of California, Davis

Date Published: August 20, 2007  |  doi:10.3791/259  |  Section: JoVE General

Keywords: Cellular Biology, indium tin oxide, surface modification, electrochemistry, cell patterning

Fabrication of a Microfluidic Device for the Compartmentalization of Neuron Soma and Axons

JoVE 261

Joseph Harris¹, Hyuna Lee¹, Behrad Vahidi¹, Christina Tu², David Cribbs³, Noo Li Jeon¹, Carl Cotman³

¹Department of Biomedical Engineering, University of California, Irvine (UCI), ²Stem Cell Research Center, University of California, Irvine (UCI), ³Institute for Brain Aging and Dementia, University of California, Irvine (UCI)

Date Published: August 22, 2007  |  doi:10.3791/261  |  Section: JoVE General

Keywords: Cell Biology, Biomedical Engineering, Neuroscience, Cell Culture, Axonal Regeneration

Counting Human Neural Stem Cells

JoVE 262

Steven Marchenko, Lisa Flanagan

Department of Pathology, University of California, Irvine (UCI)

Date Published: August 22, 2007  |  doi:10.3791/262  |  Section: JoVE General

Keywords: Basic Protocols, Stem Cells, Cell Culture, Cell Counting, Hemocytometer

Passaging Human Neural Stem Cells

JoVE 263

Steven Marchenko, Lisa Flanagan

Department of Pathology, University of California, Irvine (UCI)

Date Published: August 22, 2007  |  doi:10.3791/263  |  Section: JoVE General

Keywords: Basic Protocols, Stem Cells, Cell Culture, Cell Counting, Hemocytometer

Western Blotting Using the Invitrogen NuPage Novex Bis Tris MiniGels

JoVE 264

Aubin Penna, Michael Cahalan

Department of Physiology and Biophysics, University of California, Irvine (UCI)

Date Published: August 22, 2007  |  doi:10.3791/264  |  Section: JoVE General

Keywords: Basic Protocols, Western Blot, Biochemistry, Electrophoreisis

Murine Pancreatic Islet Isolation

JoVE 255

Gregory L. Szot, Pavel Koudria, Jeffrey A. Bluestone

Diabetes Center, University of California, San Francisco - UCSF

Date Published: August 22, 2007  |  doi:10.3791/255  |  Section: JoVE General

Keywords: Immunology, Pancreatic Islets, Cell Culture, Diabetes, Ficoll Gradient

Dissection and 2-Photon Imaging of Peripheral Lymph Nodes in Mice

JoVE 265

Melanie P. Matheu¹, Ian Parker², Michael D. Cahalan¹

¹Department of Physiology and Biophysics, University of California, Irvine (UCI), ²Department of Neurobiology and Behaviour, University of California, Irvine (UCI)

Date Published: August 23, 2007  |  doi:10.3791/265  |  Section: JoVE General

Keywords: Immunology, T Lymphocytes, Lymph Node, 2-photon Imaging, Tail Vein Injections

Drawing Blood from Rats through the Saphenous Vein and by Cardiac Puncture

JoVE 266

Christine Beeton, Adriana Garcia, K. George Chandy

Department of Physiology and Biophysics, University of California, Irvine (UCI)

Date Published: August 23, 2007  |  doi:10.3791/266  |  Section: JoVE General

Keywords: Immunology, Blood Sampling Method, Rodent, Blood Draw, Heart, Pharmacokinetics, Serum, Plasma, Blood Collection, Bleeding, Hematology

Immunocytochemistry: Human Neural Stem Cells

JoVE 267

Steven Marchenko, Lisa Flanagan

Department of Pathology, University of California, Irvine (UCI)

Date Published: August 24, 2007  |  doi:10.3791/267  |  Section: JoVE General

Keywords: Basic Protocols, Stem Cells, Immunocytochemistry, Cell Culture, antibody staining

Silicon Microchips for Manipulating Cell-cell Interaction

JoVE 268

Elliot E Hui, Sangeeta N Bhatia

Laboratory for Multiscale Regenerative Technologies, MIT - Massachusetts Institute of Technology

Date Published: August 30, 2007  |  doi:10.3791/268  |  Section: JoVE General

Keywords: tissue engineering, MEMS, microfabrication, microenvironment, Bioengineering

Micro-dissection of Rat Brain for RNA or Protein Extraction from Specific Brain Region

JoVE 269

Kin Chiu, Wui Man Lau, Ho Tak Lau, Kwok-Fai So, Raymond Chuen-Chung Chang

Laboratory of Neurodegenerative Diseases, Department of Anatomy, LKS Faculty of Medicine, The University of Hong Kong - HKU

Date Published: August 30, 2007  |  doi:10.3791/269  |  Section: JoVE General

Keywords: Neuroscience, brain, dissection

A Microfluidic Device with Groove Patterns for Studying Cellular Behavior

JoVE 270

Bong Geun Chung, Amir Manbachi, Ali Khademhosseini

Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology; Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital

Date Published: August 30, 2007  |  doi:10.3791/270  |  Section: JoVE General

Keywords: Cell Biology, tissue engineering, microfluidic, apoptosis

A Gradient-generating Microfluidic Device for Cell Biology

JoVE 271

Bong Geun Chung, Amir Manbachi, Wajeeh Saadi, Francis Lin, Noo Li Jeon, Ali Khademhosseini

Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology; Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital

Date Published: August 30, 2007  |  doi:10.3791/271  |  Section: JoVE General

Keywords: Cell Biology, tissue engineering, microfluidic, cell migration, gradient

Experimental Approaches to Tissue Engineering

JoVE 272

Ali Khademhosseini

Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology; Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital

Date Published: August 30, 2007  |  doi:10.3791/272  |  Section: JoVE General

Keywords: Cell Biology, tissue engineering, microfluidics, stem cells