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The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.

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Table of Contents

Table of Contents: Issue 46, December 2010

Jump to:  JoVE General (22) JoVE Neuroscience (11) JoVE Immunology and Infection (10) JoVE Clinical and Translational Medicine (3) JoVE Bioengineering (9) JoVE Errata (2)
 JoVE General

Expression, Detergent Solubilization, and Purification of a Membrane Transporter, the MexB Multidrug Resistance Protein


JoVE 2134

,

Department of Biological Sciences, University of Illinois Chicago - UIC

Date Published: December 3, 2010  |  doi:10.3791/2134  |  Section: JoVE General

Keywords: Cellular Biology, multidrug resistance, membrane protein, purification, transmembrane transport, MexB, detergent solubilization, protein detergent complex

 JoVE General

Bioenergetic Profile Experiment using C2C12 Myoblast Cells


JoVE 2511

1, 2, 3, 3, 3, 3

1Buck Institute for Age Research, Novato, CA, 2Department of Pathology, Center for Free Radical Biology, University of Alabama at Birmingham - UAB, 3Seahorse Bioscience, North Billerica, MA

Date Published: December 6, 2010  |  doi:10.3791/2511  |  Section: JoVE General

Keywords: Cellular Biology, Mitochondrial dysfunction, cellular, bioenergetics, metabolism, cancer, obesity, diabetes, aging, neurodegeneration

 JoVE General

Immunofluorescent Detection of Two Thymidine Analogues (CldU and IdU) in Primary Tissue


JoVE 2166

*, *, , , , , , , , , ,

Division of Endocrinology and Diabetes, Children’s Hospital of Philadelphia, Institute of Diabetes Obesity and Metabolism, Institute for Regenerative Medicine, Department of Pediatrics, University of Pennsylvania-School of Medicine

Date Published: December 7, 2010  |  doi:10.3791/2166  |  Section: JoVE General

Keywords: Immunology, CldU, IDU, Thymidine Analogue, immunofluorescence, Lineage-Tracing, Mouse, Cell Division, Replication, Turnover, Stem Cell

 JoVE General

Analysis of Pluripotent Stem Cells by using Cryosections of Embryoid Bodies


JoVE 2344

*, *, *, ,

Laboratório Nacional de Céulas-Tronco Embrionárias (LaNCE), Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro (UFRJ), Brazil

Date Published: December 8, 2010  |  doi:10.3791/2344  |  Section: JoVE General

Keywords: Developmental Biology, Embryonic stem cells, embryoid body, cryosections, immunochytochemistry, H9

 JoVE General

Peptides from Phage Display Library Modulate Gene Expression in Mesenchymal Cells and Potentiate Osteogenesis in Unicortical Bone Defects


JoVE 2362

1, 1, 1, 2, 3, 1, 1

1Orthopaedics Research, University of Virginia, 2Biological Sciences, University of Delaware, 3Orthopaedic Surgery, University of Virginia

Date Published: December 10, 2010  |  doi:10.3791/2362  |  Section: JoVE General

Keywords: Cellular Biology, osteogenesis, peptide, bone repair, anabolic effect

 JoVE General

Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry (CCMS)


JoVE 2264

1, 2, 1, 1, 2, 1, 1

1Department of Biochemistry / Analytics, caprotec bioanalytics GmbH, 2Institute of Organic Chemistry, RWTH Aachen University

Date Published: December 20, 2010  |  doi:10.3791/2264  |  Section: JoVE General

Keywords: Biochemistry, Capture Compound, photo-crosslink, small molecule-protein interaction, methyltransferase, S-adenosyl-l-homocysteine, SAH, S-adenosyl-l-methionine, SAM, functional proteomics, LC-MS/MS

 JoVE General

Testing Protozoacidal Activity of Ligand-lytic Peptides Against Termite Gut Protozoa in vitro (Protozoa Culture) and in vivo (Microinjection into Termite Hindgut)


JoVE 2190

*, *, ,

Department of Entomology, Louisiana State University Agricultural Center

Date Published: December 29, 2010  |  doi:10.3791/2190  |  Section: JoVE General

Keywords: Microbiology, Isoptera, Coptotermes formosanus, Formosan subterranean termite, termite control, paratransgenesis, symbionts, anaerobic, fluorescence

 JoVE General

Large Scale Zebrafish-Based In vivo Small Molecule Screen


JoVE 2243

1, 1, 1, 1,2,3,4

1Division of Cardiovascular Medicine, Department of Medicine, Vanderbilt University School of Medicine, 2Department of Pharmacology, Vanderbilt University School of Medicine, 3Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, 4Research Medicine, Veterans Affairs TVHS, Vanderbilt University School of Medicine

Date Published: December 30, 2010  |  doi:10.3791/2243  |  Section: JoVE General

Keywords: Developmental Biology, Chemical screen, chemical genetics, drug discovery, small molecule library, phenotype, zebrafish

 JoVE Neuroscience

Targeting of Deep Brain Structures with Microinjections for Delivery of Drugs, Viral Vectors, or Cell Transplants


JoVE 2082

1, 2, 2

1 Neuroscience Lab/ Fac. Psicologia, University of Colima, 2Department of Neurosurgery, Johns Hopkins University

Date Published: December 1, 2010  |  doi:10.3791/2082  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Microinjections, Drug Delivery Systems, Micromanipulation, Demyelination

 JoVE Neuroscience

An Organotypic Slice Assay for High-Resolution Time-Lapse Imaging of Neuronal Migration in the Postnatal Brain


JoVE 2486

, ,

Department of Molecular Biomedical Sciences, Center for Comparative Medicine and Translational Research, College of Veterinary Medicine, North Carolina State University

Date Published: December 11, 2010  |  doi:10.3791/2486  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Rostral Migratory Stream, Neuronal Migration, Organotypic Slices, Transplantation,

 JoVE Neuroscience

A Novel Method for Assessing Proximal and Distal Forelimb Function in the Rat: the Irvine, Beatties and Bresnahan (IBB) Forelimb Scale


JoVE 2246

, , , , ,

Department of Neurological Surgery, University of California, San Francisco

Date Published: December 16, 2010  |  doi:10.3791/2246  |  Section: JoVE Neuroscience

Keywords: Neuroscience, spinal cord injury, recovery of function, forelimb function, neurological test, cervical injuries

 JoVE Neuroscience

Combining Computer Game-Based Behavioural Experiments With High-Density EEG and Infrared Gaze Tracking


JoVE 2320

1,2, 1,3

1Department of Human Development, Cornell University, 2Social Sciences Division, University of Chicago, 3National Brain Research Centre, Manesar, India

Date Published: December 16, 2010  |  doi:10.3791/2320  |  Section: JoVE Neuroscience

Keywords: Neuroscience, High-density EEG, ERP, ICA, gaze tracking, computer game, ecological validity

 JoVE Neuroscience

Localizing Protein in 3D Neural Stem Cell Culture: a Hybrid Visualization Methodology


JoVE 2483

*1, *2, 2, 1

1Neural Regeneration Laboratory and Ottawa Institute of Systems Biology, Department of Biochemistry, Microbiology and Immunology, University of Ottawa, 2Carleton Immersive Media Studio, Azrieli School of Architecture and Urbanism, Carleton University

Date Published: December 19, 2010  |  doi:10.3791/2483  |  Section: JoVE Neuroscience

Keywords: Neuroscience, neural stem cell, hippocampus, cryosectioning, 3D modelling, neurosphere, Maya, compositing

 JoVE Neuroscience

Combining Transcranial Magnetic Stimulation and fMRI to Examine the Default Mode Network


JoVE 2271

, , ,

Berenson-Allen Center for Noninvasive Brain Stimulation, Beth Israel Deaconess Medical Center

Date Published: December 28, 2010  |  doi:10.3791/2271  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Transcranial Magnetic Stimulation, rTMS, fMRI, Default Mode Network, functional connectivity, resting state

 JoVE Neuroscience

TMS: Using the Theta-Burst Protocol to Explore Mechasnism of Plasticity in Individuals with Fragile X Syndrome and Autism


JoVE 2272

, ,

Berenson-Allen Center for Noninvasive Brain Stimulation, Beth Israel Deaconess Medical Center

Date Published: December 28, 2010  |  doi:10.3791/2272  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Transcranial Magnetic Stimulation, Theta-Burst Stimulation, Neural Plasticity, Fragile X, Autism

 JoVE Neuroscience

State-Dependency Effects on TMS: A Look at Motive Phosphene Behavior


JoVE 2273

1, 1, 2, 1

1Berenson-Allen Center for Noninvasive Brain Stimulation, Beth Israel Deaconess Medical Center, 2Brain Research Unit, Low Temperature Laboratory and Advanced magnetic Imaging Center, Aalto University School of Science and Technology

Date Published: December 28, 2010  |  doi:10.3791/2273  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Transcranial Magnetic Stimulation, state dependency, motive phosphenes, visual priming, V1/V2, V5/MT+

 JoVE Immunology and Infection

Non-invasive Imaging of Leukocyte Homing and Migration in vivo


JoVE 2062

1, 1,2, 1, 1, 1

1Department of Pathology and Immunology, Washington University in St. Louis, 2National Institute of Neurological Disorders and Stroke, NINDS, NIH - National Institute of Health

Date Published: December 5, 2010  |  doi:10.3791/2062  |  Section: JoVE Immunology and Infection

Keywords: Immunology, issue 46, Two-photon microscopy, leukocytes, homing, inflammation

 JoVE Immunology and Infection

Development of a Negative Selectable Marker for Entamoeba histolytica


JoVE 2410

, , ,

Division of Infectious Disease and International Health, University of Virginia Health System

Date Published: December 12, 2010  |  doi:10.3791/2410  |  Section: JoVE Immunology and Infection

Keywords: Infectious Disease, Entamoeba, negative selectable marker, 5-fluorocytosine, gene knockout, Cytosine deaminase, UPRT CMFDA.

 JoVE Immunology and Infection

Colon Ascendens Stent Peritonitis (CASP) - a Standardized Model for Polymicrobial Abdominal Sepsis


JoVE 2299

*, *, , , , , ,

Department of Surgery, University of Greifswald

Date Published: December 18, 2010  |  doi:10.3791/2299  |  Section: JoVE Immunology and Infection

Keywords: Immunology, sepsis model, sepsis, peritonitis, mice, surgery, CASP

 JoVE Immunology and Infection

Real-time Imaging of Leukotriene B4 Mediated Cell Migration and BLT1 Interactions with β-arrestin


JoVE 2315

,

Microbiology and Immunology, James Graham Brown Cancer Center, University of Louisville

Date Published: December 23, 2010  |  doi:10.3791/2315  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Live cell imaging, Chemotaxis, G-protein coupled receptor, receptor internalization, leukotriene B4, leukotriene B4 receptor 1

 JoVE Immunology and Infection

Transfection and Mutagenesis of Target Genes in Mosquito Cells by Locked Nucleic Acid-modified Oligonucleotides


JoVE 2355

1, 1, 1, 2, 1

1Department of Medical Microbiology and Immunology, University of California, Davis, 2Département Génétique et Développement, Institut Cochin, Université Paris Descartes

Date Published: December 26, 2010  |  doi:10.3791/2355  |  Section: JoVE Immunology and Infection

Keywords: Infectious Disease, Anopheles, transfection, oligonucleotides, mosquito

 JoVE Clinical and Translational Medicine

Orthotopic Aortic Transplantation: A Rat Model to Study the Development of Chronic Vasculopathy


JoVE 1989

1, 1,2, 1, 2, 1, 1,2

1University Heart Center Hamburg, Transplant and Stem Cell Immunobiology Lab (TSI), University Hospital Hamburg, 2Stanford University School of Medicine

Date Published: December 4, 2010  |  doi:10.3791/1989  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, chronic rejection, transplantation, rat, transplant vasculopathy

 JoVE Clinical and Translational Medicine

Dual-mode Imaging of Cutaneous Tissue Oxygenation and Vascular Function


JoVE 2095

1, 2, 1, 1, 1, 2, 3, 3, 3,4, 3

1Department of Biomedical Engineering, The Ohio State University, 2Department of Biomedical Informatics, The Ohio State University, 3Comprehensive Wound Center, The Ohio State University, 4Department of Surgery, The Ohio State University

Date Published: December 8, 2010  |  doi:10.3791/2095  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Dual-mode, multispectral imaging, infrared imaging, cutaneous tissue oxygenation, vascular function, co-registration, wound healing

 JoVE Clinical and Translational Medicine

Ex Vivo Culture of Patient Tissue & Examination of Gene Delivery


JoVE 2378

1, 1, 2, 2, 2, 1, 1

1Cork Cancer Research Centre, Mercy University Hospital and Leslie C. Quick Jnr. Laboratory, University College Cork, 2Department of Computer Science, University College Cork

Date Published: December 20, 2010  |  doi:10.3791/2378  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Bioluminescent imaging, Ex vivo tissue model, Preclinical research, Gene delivery

 JoVE Bioengineering

Improved Visualization and Quantitative Analysis of Drug Effects Using Micropatterned Cells


JoVE 2514

1, 1, 1, 2, 1, 1, 1, 1, 1, 1

1CYTOO Cell Architects, Grenoble, France, 2Centre Commun de Quantimétrie, Faculté de Médecine Rockefeller, Lyon, France

Date Published: December 2, 2010  |  doi:10.3791/2514  |  Section: JoVE Bioengineering

Keywords: Cellular Biology, Adhesive micropatterns, cell normalization, High Content Analysis, actin, image analysis, cytoskeleton, blebbistatin, cell-based assay, cell imaging, drug screening

 JoVE Bioengineering

Methods Development for Blood Borne Macrophage Carriage of Nanoformulated Antiretroviral Drugs


JoVE 2460

*, *, , , ,

Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center

Date Published: December 9, 2010  |  doi:10.3791/2460  |  Section: JoVE Bioengineering

Keywords: Immunology, NanoART, antiretroviral, HIV/AIDS, monocytes/macrophages, wet milling, homogenization, ultrasonication

 JoVE Bioengineering

Registered Bioimaging of Nanomaterials for Diagnostic and Therapeutic Monitoring


JoVE 2459

1, 1, 1, 1, 2, 2, 2

1Department of Radiology, University of Nebraska Medical Center, 2Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center

Date Published: December 9, 2010  |  doi:10.3791/2459  |  Section: JoVE Bioengineering

Keywords: Infectious Disease, neuroimaging, mouse, magnetic resonance imaging, magnetic resonance spectroscopy

 JoVE Bioengineering

Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells


JoVE 2177

1, 1, 1, 2, 1, 2, 1, 2, 2, 2, 1

1Institute of Biomaterials and Biomedical Engineering / Department of Chemical Engineering and Applied Chemistry, University of Toronto, 2Institute of Biomaterials and Biomedical Engineering, University of Toronto

Date Published: December 13, 2010  |  doi:10.3791/2177  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Tissue engineering, micro-tissue, endothelial cells, collagen gels, modules, 3D tissue culture.

 JoVE Bioengineering

Preparation of Complaint Matrices for Quantifying Cellular Contraction


JoVE 2173

1, 1, 2, 3, 1,2

1Institute for Biophysical Dynamics, University of Chicago, 2Physics Department - James Franck Institute, University of Chicago, 3Interdisciplinary Scientist Training Program, University of Chicago

Date Published: December 14, 2010  |  doi:10.3791/2173  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Traction force microscopy, cellular adhesion, polyacrylamide gel, stiffness, elastic modulus

 JoVE Bioengineering

Micropatterned Surfaces to Study Hyaluronic Acid Interactions with Cancer Cells


JoVE 2413

,

Department of Chemical and Biomolecular Engineering, Johns Hopkins Physical Sciences Oncology Center and Institute for NanoBioTechnology, Johns Hopkins University

Date Published: December 22, 2010  |  doi:10.3791/2413  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Hyaluronic acid, microcontact printing, carbodiimide chemistry, cancer, cell adhesion

 JoVE Bioengineering

Microfabricated Platforms for Mechanically Dynamic Cell Culture


JoVE 2224

1,2, 1,2, 1,2,3

1Department of Mechanical and Industrial Engineering, University of Toronto, 2Institute of Biomaterials and Biomedical Engineering, University of Toronto, 3Faculty of Dentistry, University of Toronto

Date Published: December 26, 2010  |  doi:10.3791/2224  |  Section: JoVE Bioengineering

Keywords: Bioengineering, cell culture, tissue engineering, mechanics, photopatterns, extracellular matrix, hydrogel, 3D cell culture

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