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The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.

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Table of Contents

Table of Contents: Issue 64, June 2012

Jump to:  JoVE Editorial (1) JoVE General (17) JoVE Neuroscience (9) JoVE Immunology and Infection (7) JoVE Clinical and Translational Medicine (9) JoVE Bioengineering (9) JoVE Errata (1)
 JoVE General

Ovariectomy and 17β-estradiol Replacement in Rats and Mice: A Visual Demonstration


JoVE 4013

, , , ,

Clinical Chemistry and Neurosurgery, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University

Date Published: June 7, 2012  |  doi:10.3791/4013  |  Section: JoVE General

Keywords: Medicine, Physiology, Oophorectomy, Rat, Mouse, 17β-estradiol, Administration, Silastic capsules, Nutella

 JoVE General

Efficient Differentiation of Mouse Embryonic Stem Cells into Motor Neurons


JoVE 3813

, , ,

Nemours Biomedical Research, Alfred I. duPont Hospital for Children

Date Published: June 9, 2012  |  doi:10.3791/3813  |  Section: JoVE General

Keywords: Stem Cell Biology, Molecular Biology, Developmental Biology, Mouse embryonic stem cells, motor neurons, spinal cord, Hb9, neurosciences, retinoic acid, sonic hedgehog, Islet-1, choline acetyltransferase

 JoVE General

Basic Caenorhabditis elegans Methods: Synchronization and Observation


JoVE 4019

1,2, 1, 1,2, 1,2

1Department of Cancer and Human Molecular Genetics, Bellvitge Institute for Biomedical Research, 2C. elegans Core Facility, Bellvitge Institute for Biomedical Research

Date Published: June 10, 2012  |  doi:10.3791/4019  |  Section: JoVE General

Keywords: Basic Protocols, Genetics, Developmental Biology, Molecular Biology, C. elegans, synchronization, development, Nomarski, DAPI staining

 JoVE General

Genome Editing with CompoZr Custom Zinc Finger Nucleases (ZFNs)


JoVE 3304

, , , , ,

Emerging Technologies, Sigma Life Science

Date Published: June 14, 2012  |  doi:10.3791/3304  |  Section: JoVE General

Keywords: Genetics, Molecular Biology, Zinc Finger Nuclease, Genome Engineering, Genomic Editing, Gene Modification, Gene Knockout, Gene Integration, non-homologous end joining, homologous recombination, targeted genome editing

 JoVE General

Studying Mitotic Checkpoint by Illustrating Dynamic Kinetochore Protein Behavior and Chromosome Motion in Living Drosophila Syncytial Embryos


JoVE 3763

,

Institute for Cell and Molecular Biosciences, University of Newcastle, United Kingdom

Date Published: June 14, 2012  |  doi:10.3791/3763  |  Section: JoVE General

Keywords: Cellular Biology, Developmental Biology, Spindle assembly checkpoint (SAC), Mitosis, Laser scanning confocal microscopy system, Kinetochore, Drosophila melanogaster, Syncytial embryo

 JoVE General

Isolation of Immune Cells from Primary Tumors


JoVE 3952

1, 1, 2, 1

1Tumor Immunity and Tolerance Section, Laboratory of Molecular Immunoregulation, Cancer and Inflammation Program, National Cancer Institute - Frederick, 2KEWB Productions

Date Published: June 16, 2012  |  doi:10.3791/3952  |  Section: JoVE General

Keywords: Cancer Biology, Immunology, Prostate, tumor, immune cell isolation, mouse, TRAMP, B16 melanoma, leukocyte, dendritic cell, T cell

 JoVE General

Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics


JoVE 3487

,

Molekulare Pflanzenphysiologie, Max-Planck-Institut

Date Published: June 17, 2012  |  doi:10.3791/3487  |  Section: JoVE General

Keywords: Plant Biology, Genetics, Bioinformatics, Metabolomics, Plant metabolism, Transcriptome analysis, Functional annotation, Computational biology, Plant biology, Theoretical biology, Spectroscopy and structural analysis

 JoVE General

Preparation of Mouse Embryonic Fibroblast Cells Suitable for Culturing Human Embryonic and Induced Pluripotent Stem Cells


JoVE 3854

, ,

Department of Vertebrate Genomics, Max Planck Institute for Molecular Genetics

Date Published: June 21, 2012  |  doi:10.3791/3854  |  Section: JoVE General

Keywords: Stem Cell Biology, Molecular Biology, Developmental Biology, mouse embryonic fibroblasts (MEFs), human embryonic stem cells (hESCs), human induced pluripotent stem cells (hiPSCs), Activin A -conditioned medium (CM), cell culture

 JoVE General

Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER


JoVE 3943

1, 1, 2, 1, 1,3

1Center for Genome Sciences and Systems Biology, Department of Genetics, Washington University School of Medicine, 2Department of Internal Medicine, Washington University School of Medicine, 3Department of Pediatrics, Washington University School of Medicine

Date Published: June 23, 2012  |  doi:10.3791/3943  |  Section: JoVE General

Keywords: Genetics, Genomics, Cancer Biology, Bioinformatics, Pooled DNA sequencing, SPLINTER, rare genetic variants, genetic screening, phenotype, high throughput, computational analysis, DNA, PCR, primers

 JoVE General

Single Oocyte Bisulfite Mutagenesis


JoVE 4046

1,2,3, 3, 1,2,3

1Department of Obstretrics & Gynaecology, Schulich School of Medicine and Dentistry, University of Western Ontario, 2Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, 3Children's Health Research Institute

Date Published: June 27, 2012  |  doi:10.3791/4046  |  Section: JoVE General

Keywords: Genetics, Developmental Biology, Biochemistry, Bisulfite mutagenesis, DNA methylation, individual oocyte, individual embryo, mouse model, PCR, epigenetics

 JoVE General

Isolation of Rat Portal Fibroblasts by In situ Liver Perfusion


JoVE 3669

1, 2, 2, 2

1Division of Gastroenterology, Hepatology & Nutrition, Department of Pediatrics, The Children's Hospital of Philadelphia, 2Department of Medicine, University of Pennsylvania

Date Published: June 29, 2012  |  doi:10.3791/3669  |  Section: JoVE General

Keywords: Physiology, Medicine, Liver, fibrosis, portal fibroblast, liver perfusion, myofibroblast, biliary fibrosis

 JoVE General

Isolation and Primary Culture of Rat Hepatic Cells


JoVE 3917

1, 2, 3, 1

1Department of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, 2American University in Washington, D.C., 3Department of Internal Medicine, Saint Louis University School of Medicine

Date Published: June 29, 2012  |  doi:10.3791/3917  |  Section: JoVE General

Keywords: Cellular Biology, Physiology, Medicine, Primary cell culture, hepatic cells, hepatocyte, rat

 JoVE Neuroscience

Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube


JoVE 4134

1, 1,2, 3

1Department of Cell and Developmental Biology, Center for Stem Cell Biology, Vanderbilt University Medical Center, 2Department of Pharmacology, Center for Stem Cell Biology, Vanderbilt University Medical Center, 3Vanderbilt University Medical Center

Date Published: June 2, 2012  |  doi:10.3791/4134  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Developmental Biology, neural crest, explant, cell culture, mouse, embryo

 JoVE Neuroscience

Visualization of Proprioceptors in Drosophila Larvae and Pupae


JoVE 3846

, ,

Department of Genetics and the Rappaport Institute for Research in the Medical Sciences, Rappaport Faculty of Medicine, Technion-Israel Institute of Technology

Date Published: June 13, 2012  |  doi:10.3791/3846  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Developmental Biology, Proprioceptors, chordotonal organs, wing, haltere, Drosophila, immunohistochemistry, pupae, larvae

 JoVE Neuroscience

Multimodal Imaging of Stem Cell Implantation in the Central Nervous System of Mice


JoVE 3906

1,2, 1, 2, 1,2, 1, 1, 1, 1, 2, 1

1Laboratory of Experimental Hematology, University of Antwerp, 2Bio Imaging Lab, University of Antwerp

Date Published: June 13, 2012  |  doi:10.3791/3906  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Stem cell biology, Cell labeling, Cell Transplantation, Brain, Bioluminescence Imaging, Magnetic Resonance Imaging, Histology

 JoVE Neuroscience

Correlating Behavioral Responses to fMRI Signals from Human Prefrontal Cortex: Examining Cognitive Processes Using Task Analysis


JoVE 3237

1,2, 1, 1

1Department of Psychology, Centre for Vision Research, York University, 2Department of Biology, Centre for Vision Research, York University

Date Published: June 20, 2012  |  doi:10.3791/3237  |  Section: JoVE Neuroscience

Keywords: Neuroscience, fMRI, eyetracking, BOLD, attention, inhibition, Magnetic Resonance Imaging, MRI

 JoVE Neuroscience

Derivation of Glial Restricted Precursors from E13 mice


JoVE 3462

1,2, 1,2, 1,3, 2, 1, 1, 4, 2,5, 1,2,6, 1,2,6

1Hugo W. Moser Research Institute at Kennedy Krieger, Johns Hopkins University, 2Department of Neurology, Johns Hopkins School of Medicine, 3University of Maryland, 4Experimental Neurology, Biogen Idec, 5The Brain Science Institute, Johns Hopkins School of Medicine, 6Department of Pediatrics, Johns Hopkins School of Medicine

Date Published: June 20, 2012  |  doi:10.3791/3462  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Physiology, Medicine, periventricular leukomalacia, oligodendrocytes, glial restricted precursors, spinal cord, cell culture

 JoVE Neuroscience

Preparation of an Awake Mouse for Recording Neural Responses and Injecting Tracers


JoVE 3755

1,2, 3, 2,4, 3

1Department of Neuroscience, Johns Hopkins University, 2Garvan Institute of Medical Research, 3School of Biological Sciences, Washington State University, 4Department of Otolaryngology-HNS, Johns Hopkins University

Date Published: June 26, 2012  |  doi:10.3791/3755  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Physiology, brain, auditory system, mouse, electrophysiology, recordings, dye injections, neuron labeling

 JoVE Neuroscience

Recording Human Electrocorticographic (ECoG) Signals for Neuroscientific Research and Real-time Functional Cortical Mapping


JoVE 3993

1, 1,2, 1,2, 1,2, 3, 2, 1,2,4,5,6,7

1Wadsworth Center, New York State Department of Health, 2Department of Neurology, Albany Medical College, 3Department of Neurosurgery, Albany Medical College, 4Department of Neurosurgery, Washington University, 5Department of Biomed. Eng., Rensselaer Polytechnic Institute, 6Department of Biomed. Sci., State University of New York at Albany, 7Department of Elec. and Comp. Eng., University of Texas at El Paso

Date Published: June 26, 2012  |  doi:10.3791/3993  |  Section: JoVE Neuroscience

Keywords: Neuroscience, electrocorticography, brain-computer interfacing, functional brain mapping, SIGFRIED, BCI2000, epilepsy monitoring, magnetic resonance imaging, MRI

 JoVE Immunology and Infection

Experimental Endocarditis Model of Methicillin Resistant Staphylococcus aureus (MRSA) in Rat


JoVE 3863

1, 1,2, 1,2

1Department of Medicine, Division of Infectious Diseases, Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center, 2Geffen School of Medicine at UCLA

Date Published: June 4, 2012  |  doi:10.3791/3863  |  Section: JoVE Immunology and Infection

Keywords: Infection, Immunology, Staphylococcus aureus, endocarditis, animal model, methicillin resistance, MRSA, rat

 JoVE Immunology and Infection

Use of Artificial Sputum Medium to Test Antibiotic Efficacy Against Pseudomonas aeruginosa in Conditions More Relevant to the Cystic Fibrosis Lung


JoVE 3857

1, 2, 1, 1, 1, 1

1Institute of Infection and Global Health, University of Liverpool, 2NIHR Biomedical Research Centre in Microbial Disease, University of Liverpool

Date Published: June 5, 2012  |  doi:10.3791/3857  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Microbiology, Pseudomonas aeruginosa, antimicrobial susceptibility, artificial sputum media, lung infection, cystic fibrosis, diagnostics, plankton

 JoVE Immunology and Infection

Using Eggs from Schistosoma mansoni as an In vivo Model of Helminth-induced Lung Inflammation


JoVE 3905

1, 2, 2, 1

1Institute of Immunology, Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, 2Pathobiology, School of Veterinary Medicine, University of Pennsylvania

Date Published: June 5, 2012  |  doi:10.3791/3905  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Infection, Microbiology, helminth, parasite, mouse, Th2, lung, inflammation, granuloma, alternative activation, macrophage

 JoVE Immunology and Infection

Purification of Pathogen Vacuoles from Legionella-infected Phagocytes


JoVE 4118

, ,

Max von Pettenkofer-Institut, Ludwig-Maximilians-Universität

Date Published: June 19, 2012  |  doi:10.3791/4118  |  Section: JoVE Immunology and Infection

Keywords: Infection, Immunology, amoeba, Dictyostelium discoideum, density gradient centrifugation, effector protein, Icm/Dot type IV secretion system, immuno-magnetic separation, Legionella pneumophila, macrophage, pathogen vacuole

 JoVE Immunology and Infection

Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes


JoVE 3709

1, 1,2

1Department of Molecular Biology and Biochemistry, University of California, Irvine, 2Department of Microbiology and Molecular Genetics, University of California, Irvine

Date Published: June 28, 2012  |  doi:10.3791/3709  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Molecular Biology, Biochemistry, Genetics, Developmental Biology, Hybridization in situ, RNA localization, salivary glands, ovary, embryo, mosquito

 JoVE Clinical and Translational Medicine

DNA Vector-based RNA Interference to Study Gene Function in Cancer


JoVE 4129

1, 1, 1, 2, 1

1Department of Cancer Biology and Comprehensive Cancer Center, Wake Forest University School of Medicine, 2Department of Pathology and Comprehensive Cancer Center, Wake Forest University School of Medicine

Date Published: June 4, 2012  |  doi:10.3791/4129  |  Section: JoVE Clinical and Translational Medicine

Keywords: Cancer Biology, Medicine, Genetics, RNAi, shRNA, gene silencing, mouse xenograft, tumor formation

 JoVE Clinical and Translational Medicine

Roux-en-Y Gastric Bypass Operation in Rats


JoVE 3940

1,2, 2,3, 4, 2,3, 4

1Department of Surgery, University Hospital Zürich, 2Zürich Centre for Integrative Human Physiology, University of Zürich, 3Institute of Veterinary Physiology, Vetsuisse Faculty, University of Zürich, 4Imperial Weight Centre, Department of Investigative Medicine, Imperial College London

Date Published: June 11, 2012  |  doi:10.3791/3940  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Physiology, Roux-en-Y Gastric bypass, rat model, gastric pouch size, gut hormones

 JoVE Clinical and Translational Medicine

Corneal Donor Tissue Preparation for Endothelial Keratoplasty


JoVE 3847

1, 2, 2, 1

1Department of Ophthalmology, University of Michigan, 2MidWest Eye Banks

Date Published: June 12, 2012  |  doi:10.3791/3847  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Physiology, Cornea, transplantation, DSAEK, DSEK, endothelial keratoplasty, lamellar, graft, Moria, microkeratome, precut, Fuchs dystrophy

 JoVE Clinical and Translational Medicine

A Simplified Technique for In situ Excision of Cornea and Evisceration of Retinal Tissue from Human Ocular Globe


JoVE 3765

1, 1, 1, 1, 1, 2, 1, 1

1Fondazione Banca Degli Occhi del Veneto O.N.L.U.S., 2Telethon Institute for Genetics & Medicine (T.I.G.E.M.)

Date Published: June 12, 2012  |  doi:10.3791/3765  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Physiology, Human cadaver ocular globe, in situ excision, corneal tissue, in situ evisceration, retinal tissue

 JoVE Clinical and Translational Medicine

Analytical Techniques for Assaying Nitric Oxide Bioactivity


JoVE 3722

1, 1, 1, 2, 1

1Texas Therapeutics Institute, University of Texas Health Science Center at Houston, 2Deptartment of Pediatrics, Baylor College of Medicine

Date Published: June 18, 2012  |  doi:10.3791/3722  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Molecular Biology, Nitric oxide, nitrite, nitrate, endothelium derived relaxing factor, HPLC, chemiluminscence

 JoVE Clinical and Translational Medicine

Preparation of Myeloid Derived Suppressor Cells (MDSC) from Naive and Pancreatic Tumor-bearing Mice using Flow Cytometry and Automated Magnetic Activated Cell Sorting (AutoMACS)


JoVE 3875

, , ,

Department of Molecular Medicine, University of South Florida Morsani College of Medicine

Date Published: June 18, 2012  |  doi:10.3791/3875  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Cancer Biology, Immunology, MDSC, Immunophenotyping, Flow Cytometry, AutoMACS, FACS

 JoVE Clinical and Translational Medicine

Assessing Replication and Beta Cell Function in Adenovirally-transduced Isolated Rodent Islets


JoVE 4080

1,2, 2, 2, 1,2

1Department of Pediatrics, Indiana University School of Medicine, 2Department of Cellular & Integrative Physiology, Indiana University School of Medicine

Date Published: June 25, 2012  |  doi:10.3791/4080  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Physiology, beta cell, gene expression, islet, diabetes, insulin secretion, proliferation, adenovirus, rat

 JoVE Bioengineering

Constructing a Collagen Hydrogel for the Delivery of Stem Cell-loaded Chitosan Microspheres


JoVE 3624

*, *,

Department of Regenerative Medicine, United States Army Institute of Surgical Research

Date Published: June 1, 2012  |  doi:10.3791/3624  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Biomedical Engineering, Tissue Engineering, chitosan, microspheres, collagen, hydrogel, cell delivery, adipose-derived stem cells, ASC, CSM

 JoVE Bioengineering

Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing


JoVE 4067

1,2, 1, 2, 2, 2, 2, 2, 1

1Joint Department of Biomedical Engineering, University of North Carolina and North Carolina State University, 2Department of Biosensors and Nanomaterials, Sandia National Laboratories

Date Published: June 1, 2012  |  doi:10.3791/4067  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Biomedical Engineering, Microneedle, Microneedle sensors, multiplexed detection, electrochemistry, stereolithography

 JoVE Bioengineering

Bridging the Bio-Electronic Interface with Biofabrication


JoVE 4231

*1, *2, *1, 3, 2, 1, 1

1Fischell Department of Bioengineering, University of Maryland, 2Institute for Bioscience and Biotechnology Research, University of Maryland, 3Department of Materials Science and Engineering, University of Maryland

Date Published: June 6, 2012  |  doi:10.3791/4231  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Biomedical Engineering, electrodeposition, biofabrication, chitosan, alginate, lab-on-a-chip, microfluidic, DTRA

 JoVE Bioengineering

Harvesting Murine Alveolar Macrophages and Evaluating Cellular Activation Induced by Polyanhydride Nanoparticles


JoVE 3883

1, 2, 2, 2, 1

1Department of Chemical and Biological Engineering, Iowa State University, 2Department of Veterinary Microbiology and Preventive Medicine, Iowa State University

Date Published: June 8, 2012  |  doi:10.3791/3883  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Microbiology, alveolar macrophages, AMɸ, lung lavage, polyanhydride, nanoparticles, harvesting, activation

 JoVE Bioengineering

Analyzing Cellular Internalization of Nanoparticles and Bacteria by Multi-spectral Imaging Flow Cytometry


JoVE 3884

1, 1, 2, 3, 1, 1, 1, 3, 1, 1

1Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, 2Amnis Corporation, 3Department of Chemical and Biological Engineering, Iowa State University

Date Published: June 8, 2012  |  doi:10.3791/3884  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Microbiology, ImageStream, phagocytosis, nanoparticles, pathogen, bacteria, Salmonella, imaging, multi-spectral imaging, flow cytometry

 JoVE Bioengineering

Rapid Isolation of Viable Circulating Tumor Cells from Patient Blood Samples


JoVE 4248

1, 1, 2,3, 2,3, 1

1Department of Biomedical Engineering, Cornell University, 2BioCytics, Inc., 3Carolina BioOncology Institute, PLLC

Date Published: June 15, 2012  |  doi:10.3791/4248  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Biomedical Engineering, Cancer Biology, Circulating tumor cells, metastasis, selectin, nanotechnology, halloysite nanotubes, cell isolation, cancer

 JoVE Bioengineering

Endothelialized Microfluidics for Studying Microvascular Interactions in Hematologic Diseases


JoVE 3958

*1,2,3,4, *1,2,3,4, 1,2,3,4, 1,2,3,4, 1,2,3,4, 1,2,3,4, 1,2,3,4, 1,2,3,4, 1,2,3,4

1Department of Pediatrics, Emory University School of Medicine, 2Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 3Aflac Cancer Center and Blood Disorders Service of Children's Healthcare of Atlanta, 4Winship Cancer Institute of Emory University

Date Published: June 22, 2012  |  doi:10.3791/3958  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Biomedical Engineering, endothelial cells, HUVEC, microfabrication, microvasculature, SU-8, micromolding, soft lithography

 JoVE Bioengineering

Constant Pressure-controlled Extrusion Method for the Preparation of Nano-sized Lipid Vesicles


JoVE 4151

*1,2, *1,2, 1,2

1Department of Chemistry & Biochemistry, University of Colorado Boulder, 2Biofrontiers Institute, University of Colorado Boulder

Date Published: June 22, 2012  |  doi:10.3791/4151  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Biomedical Engineering, Liposomes, particle extrusion, nano-sized vesicles, dynamic light scattering (DLS), nanoparticle tracking analysis (NTA)

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