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The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.

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1: October/November 2006

Table of Contents

Table of Contents: Issue 1, October/November 2006

Jump to: (17)

Passaging HuES Human Embryonic Stem Cell-lines with Trypsin

JoVE 49

Erin Trish, John Dimos, Kevin Eggan

Department of Molecular and Cell Biology, Harvard

Date Published: October 12, 2006 | doi:10.3791/49 | Section: JoVE General

Keywords: Cellular Biology, embryonic stem cells, ES, tissue culture

Freezing Human ES Cells

JoVE 50

Erin Trish, John Dimos, Kevin Eggan

Department of Molecular and Cell Biology, Harvard

Date Published: October 12, 2006 | doi:10.3791/50 | Section: JoVE General

Keywords: Cellular Biology, Embryonic Stem Cells, ES, Tissue Culture, Freezing

Dissection of Drosophila Ovaries

JoVE 52

Li Chin Wong, Paul Schedl

Princeton University

Date Published: October 19, 2006 | doi:10.3791/52 | Section: JoVE General

Keywords: Neuroscience, Protocol, Stem Cells, Cerebral Cortex, Brain Development, Electroporation, Intra Uterine Injections, transfection

Assay for Adhesion and Agar Invasion in S. cerevisiae

JoVE 64

Cemile G Guldal, James Broach

Department of Molecular Biology, Princeton University

Date Published: November 8, 2006 | doi:10.3791/64 | Section: JoVE General

Keywords: Microbiology, Yeast, Adhesion, Invasion

Monitoring Actin Disassembly with Time-lapse Microscopy

JoVE 66

Hao Yuan Kueh

Dept. of Systems Biology, Harvard Medical School

Date Published: November 8, 2006 | doi:10.3791/66 | Section: JoVE General

Keywords: Cellular Biology, cytoskeleton, actin, timelapse, filament, chamber

A Rapid Technique for the Visualization of Live Immobilized Yeast Cells

JoVE 84

Karl Zawadzki, James Broach

Department of Molecular Biology, Princeton University

Date Published: November 9, 2006 | doi:10.3791/84 | Section: JoVE General

Keywords: Microbiology, yeast, HML, HMR, epigenetic, loci, silencing, cerevisiae

Dissection of Larval CNS in Drosophila Melanogaster

JoVE 85

Nathaniel Hafer, Paul Schedl

Department of Molecular Biology, Princeton University

Date Published: November 9, 2006 | doi:10.3791/85 | Section: JoVE General

Keywords: Developmental Biology, Drosophila, fly, CNS, larvae

Human ES cells: Starting Culture from Frozen Cells

JoVE 86

Erin Trish, John Dimos, Kevin Eggan

Department of Molecular and Cell Biology, Harvard

Date Published: November 9, 2006 | doi:10.3791/86 | Section: JoVE General

Keywords: Cellular Biology, ES, embryonic stem cells

Light/dark Transition Test for Mice

JoVE 104

Keizo Takao, Tsuyoshi Miyakawa

Genetic Engineering and Functional Genomics Unit, Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University

Date Published: November 13, 2006 | doi:10.3791/104 | Section: JoVE General

Keywords: Neuroscience, knockout mice, transgenic mice, behavioral test, phenotyping

Horizontal Slice Preparation of the Retina

JoVE 108

Ryosuke Enoki¹, Tatjana C. Jakobs², Amane Koizumi²

¹Dpt of Physiology and Biophysics, Dalhousie University, ²Massachusetts General Hospital, Harvard Medical School

Date Published: November 20, 2006 | doi:10.3791/108 | Section: JoVE General

Keywords: Neuroscience, retina, dissection

Gibberella zeae Ascospore Production and Collection for Microarray Experiments.

JoVE 115

Matias Pasquali^1,2, Corby Kistler³

¹Cereal Disease Laboratory, USDA, ²University of Minnesota/ Agroinnova, University of Torino, ³Cereal Disease Laboratory, University of Minnesota

Date Published: November 30, 2006 | doi:10.3791/115 | Section: JoVE General

Keywords: Plant Biology, sexual cross, spore separation, MIAME standards

Nuclear Transfer into Mouse Oocytes

JoVE 116

Dieter Egli, Kevin Eggan

Department of Molecular and Cell Biology, Harvard

Date Published: November 30, 2006 | doi:10.3791/116 | Section: JoVE General

Keywords: Developmental Biology, oocytes, nuclear transfer, stem cells

Measuring Exocytosis in Neurons Using FM Labeling

JoVE 117

Jamila Newton, Venkatesh Murthy

Department of Molecular and Cell Biology, Harvard

Date Published: November 30, 2006 | doi:10.3791/117 | Section: JoVE General

Keywords: Neuroscience, neuron, imaging, exocytosis

ES Cell-derived Neuroepithelial Cell Cultures

JoVE 118

Shreeya Karki, Jan Pruszak, Ole Isacson, Kai C Sonntag

McLean Hospital, Harvard Medical School

Date Published: November 30, 2006 | doi:10.3791/118 | Section: JoVE General

Keywords: Cellular Biology, issue 1, embryonic stem (ES) cells, rosettes, neuroepithelial precursors, stromal cells, differentiation

Propagation of Human Embryonic Stem (ES) Cells

JoVE 119

Laurence Daheron

Center for Regenerative Medicine, MGH - Massachusetts General Hospital

Date Published: November 30, 2006 | doi:10.3791/119 | Section: JoVE General

Keywords: Cellular Biology, ES, embryonic stem cells, tissue culture

Long-term Imaging Mammalian Cells using Wide-Field Microscopy

JoVE 120

Meg Bentley, Randy King

Dept. of Cell Biology, Harvard Medical School

Date Published: November 30, 2006 | doi:10.3791/120 | Section: JoVE General

Keywords: Cellular Biology, imaging, tissue culture, screen

Gene-gun Transfection of Hippocampal Neurons

JoVE 121

Powrnima Joshi, Anna Dunaevsky

Dept of Neuroscience, Brown University

Date Published: November 30, 2006 | doi:10.3791/121 | Section: JoVE General

Keywords: Neuroscience, brain, hippocampus, neuron, transfection, gene-gun