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The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.

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Table of Contents

Table of Contents: Issue 49, March 2011

Jump to:  JoVE General (23) JoVE Neuroscience (16) JoVE Immunology and Infection (4) JoVE Clinical and Translational Medicine (5) JoVE Bioengineering (4) JoVE Errata (4)
 JoVE General

Detection of Signaling Effector-Complexes Downstream of BMP4 Using in situ PLA, a Proximity Ligation Assay


JoVE 2631

,

Medical Research Council, Clinical Sciences Centre, Imperial College, Hammersmith Hospital

Date Published: March 3, 2011  |  doi:10.3791/2631  |  Section: JoVE General

Keywords: Cellular Biology, Proximity Ligation Assay, BMP, signaling, Smad4, Smad1/5, HEK293T, signaling effectors, phospho-Smads, immunocytochemistry, Antibody

 JoVE General

Computer-assisted Large-scale Visualization and Quantification of Pancreatic Islet Mass, Size Distribution and Architecture


JoVE 2471

1, 1, 1, 1, 2, 2, 3, 4, 1

1Department of Medicine, University of Chicago, 2Laboratory of Biological Modeling, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 3Department of Surgery, University of Chicago, 4Diabetes Division, University of Massachusetts

Date Published: March 4, 2011  |  doi:10.3791/2471  |  Section: JoVE General

Keywords: Cellular Biology, beta-cells, islets, large-scale analysis, pancreas

 JoVE General

Rapid PCR Thermocycling using Microscale Thermal Convection


JoVE 2366

1, 2, 3

1Department of Mechanical Engineering, Texas A&M University, 2Department of Mechanical Engineering and Department of Nuclear Engineering, Texas A&M University, 3Department of Chemical Engineering, Texas A&M University

Date Published: March 5, 2011  |  doi:10.3791/2366  |  Section: JoVE General

Keywords: Molecular Biology, polymerase chain reaction, PCR, DNA, thermal convection

 JoVE General

A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals


JoVE 2791

, ,

Department of Biology and Centre for Species at Risk and Habitat Studies,, University of British Columbia, Okanagan Campus

Date Published: March 13, 2011  |  doi:10.3791/2791  |  Section: JoVE General

Keywords: Genetics, Conservation genetics, noninvasive genetic sampling, Hair snares, Microsatellites, AFLPs, American pika, Ochotona princeps

 JoVE General

Clonogenic Assay: Adherent Cells


JoVE 2573

1,2, 1,2,3, 1, 1,4, 2,3, 1,2

1Epigenomic Medicine, BakerIDI Heart and Diabetes Institute, The Alfred Medical Research and Education Precinct, 2Department of Pathology, The University of Melbourne, 3Epigenetics in Human Health and Disease, BakerIDI Heart and Diabetes Institute, The Alfred Medical Research and Education Precinct, 4Department of Anatomy and Cellular Biology, The University of Melbourne

Date Published: March 13, 2011  |  doi:10.3791/2573  |  Section: JoVE General

Keywords: Cellular Biology, clonogenic assay, clonogenic survival, colony staining, colony counting, radiation sensitivity, radiation modification

 JoVE General

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli


JoVE 2505

, , , ,

Department of Microbiology & Environmental Toxicology, University of California Santa Cruz - UCSC

Date Published: March 16, 2011  |  doi:10.3791/2505  |  Section: JoVE General

Keywords: Genetics, random mutagenesis, directed evolution, LB agar drug gradient, bacterial complementation, ColE1 plasmid, DNA polymerase I, replication fidelity, genetic adaptation, antimicrobials, methylating agents

 JoVE General

Measuring Caenorhabditis elegans Life Span in 96 Well Microtiter Plates


JoVE 2496

1,2, 1,2

1Department of Chemical Physiology, The Scripps Research Institute, 2Department of Molecular and Experimental Medicine, The Scripps Research Institute

Date Published: March 18, 2011  |  doi:10.3791/2496  |  Section: JoVE General

Keywords: Cellular Biology, High-throughput screening, aging, lifespan, phenotype based screening, drug discovery, age-related disease

 JoVE General

DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses


JoVE 2763

*1,2, *1,2, 3, 1,2,4

1Department of Integrative Oncology, BC Cancer Research Centre, 2Interdisciplinary Oncology Program, University of British Columbia - UBC, 3Photography/Video Production, Multi-Media Services, BC Cancer Agency, 4Department of Pathology and Laboratory Medicine, University of British Columbia - UBC

Date Published: March 26, 2011  |  doi:10.3791/2763  |  Section: JoVE General

Keywords: Genetics, DNA extraction, paraffin embedded tissue, phenol:chloroform extraction, genetic analysis, epigenetic analysis

 JoVE General

Imaging Cell Shape Change in Living Drosophila Embryos


JoVE 2503

1, 1,2

1Program in Cell & Molecular Biology, Baylor College of Medicine (BCM), 2Verna & Marrs McLean Department of Biochemistry & Molecular Biology, Baylor College of Medicine (BCM)

Date Published: March 30, 2011  |  doi:10.3791/2503  |  Section: JoVE General

Keywords: Developmental Biology, confocal microscopy, live imaging, GFP, Drosophila, embryos, cell shape change, cellularization, plasma membrane invagination, morphogenesis, membrane trafficking

 JoVE Neuroscience

Combining Behavioral Endocrinology and Experimental Economics: Testosterone and Social Decision Making


JoVE 2065

*1, *2

1Institute for Empirical Research in Economics, Laboratory for Social and Neural Systems Research, University of Zurich, 2Department of Economics, Royal Holloway, University of London

Date Published: March 2, 2011  |  doi:10.3791/2065  |  Section: JoVE Neuroscience

Keywords: Neuroscience, behavioral endocrinology, testosterone, social status, decision making

 JoVE Neuroscience

Neural-Colony Forming Cell Assay: An Assay To Discriminate Bona Fide Neural Stem Cells from Neural Progenitor Cells


JoVE 2639

1,2, 3, 1, 1, 1

1Department of Neurosurgery, University of Florida, 2Department of Anatomical Sciences, Shiraz University of Medical Sciences, 3STEMCELL Technologies, Inc.

Date Published: March 6, 2011  |  doi:10.3791/2639  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Stem Cells, Neural Colony Forming Cell Assay, Progenitor Cells, enumeration

 JoVE Neuroscience

C. elegans Positive Butanone Learning, Short-term, and Long-term Associative Memory Assays


JoVE 2490

1, 2, 1, 1, 1, 1

1Department of Molecular Biology, Lewis-Sigler Institute for Integrative Genomics, Princeton University, 2Lewis-Sigler Institute for Integrative Genomics, Princeton University

Date Published: March 11, 2011  |  doi:10.3791/2490  |  Section: JoVE Neuroscience

Keywords: Neuroscience, memory, associative learning, C. elegans, chemotaxis, spaced training, behavior

 JoVE Neuroscience

Organotypic Culture of Full-thickness Adult Porcine Retina


JoVE 2655

1, 2, 2, 1

1Neurology and Neurosciences, University of Medicine and Dentistry of New Jersey - UMDNJ, 2Institute of Ophthalmology and Visual Science, University of Medicine and Dentistry of New Jersey - UMDNJ

Date Published: March 20, 2011  |  doi:10.3791/2655  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Retina, in vitro, Porcine, Photoreceptor

 JoVE Neuroscience

Preparation and Culture of Chicken Auditory Brainstem Slices


JoVE 2527

*1, *1, 1,2, 2

1Department of Otolaryngology-Head and Neck Surgery, Virginia Merrill Bloedel Hearing Research Center, University of Washington, 2Department of Physiology and Biophysics, Virginia Merrill Bloedel Hearing Research Center, University of Washington

Date Published: March 21, 2011  |  doi:10.3791/2527  |  Section: JoVE Neuroscience

Keywords: Neuroscience, slice preparation, chicken auditory brainstem, organotypic cultures, nucleus laminaris, nucleus magnocellularis

 JoVE Neuroscience

Optical Imaging of Neurons in the Crab Stomatogastric Ganglion with Voltage-sensitive Dyes


JoVE 2567

1, 1, 2

1Institute of Neurobiology, Ulm University, 2School of Computing Science & Institute of Neuroscience, Newcastle University

Date Published: March 23, 2011  |  doi:10.3791/2567  |  Section: JoVE Neuroscience

Keywords: Neuroscience, stomatogastric ganglion, voltage sensitive dye, neuron resolution imaging, central pattern generator

 JoVE Neuroscience

Preparation of Acute Hippocampal Slices from Rats and Transgenic Mice for the Study of Synaptic Alterations during Aging and Amyloid Pathology


JoVE 2330

1, 2, 2,3

1Graduate Center for Gerontology, University of Kentucky College of Public Health, 2Department of Molecular and Biomedical Pharmacology, University of Kentucky College of Medicine, 3Sanders-Brown Center on Aging, University of Kentucky College of Medicine

Date Published: March 23, 2011  |  doi:10.3791/2330  |  Section: JoVE Neuroscience

Keywords: Neuroscience, aging, amyloid, hippocampal slice, synaptic plasticity, Ca2+, CA1, electrophysiology

 JoVE Neuroscience

Organotypic Slice Culture of GFP-expressing Mouse Embryos for Real-time Imaging of Peripheral Nerve Outgrowth


JoVE 2309

,

Interdisciplinary Center for Neurosciences, Institute of Anatomy and Cell Biology, University of Heidelberg

Date Published: March 29, 2011  |  doi:10.3791/2309  |  Section: JoVE Neuroscience

Keywords: Neuroscience, imaging, organotypic slice culture, GFP, spinal nerves, mouse, developmental biology, axonal pathfinding, DRG

 JoVE Immunology and Infection

Preparation and Use of HIV-1 Infected Primary CD4+ T-Cells as Target Cells in Natural Killer Cell Cytotoxic Assays


JoVE 2668

, ,

Department of Immunology and Microbiology, Rush University Medical Center

Date Published: March 14, 2011  |  doi:10.3791/2668  |  Section: JoVE Immunology and Infection

Keywords: Immunology, innate immunity, HIV-1, natural killer cell, cytolytic assay, degranulation assay, primary lymphocytes

 JoVE Immunology and Infection

Induction of Alloantigen-specific Anergy in Human Peripheral Blood Mononuclear Cells by Alloantigen Stimulation with Co-stimulatory Signal Blockade


JoVE 2673

1,2, 1, 1, 1,2, 3,4

1Medical Oncology, Dana Farber Cancer Institute, 2Department of Medicine, Brigham and Womens Hospital, 3Pediatric Oncology, Dana Farber Cancer Institute, 4Division of Hematology/Oncology, Children’s Hospital Boston

Date Published: March 14, 2011  |  doi:10.3791/2673  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Allogeneic stem cell transplantation, alloreactivity, Graft-versus-Host Disease, T cell costimulation, anergy, mixed lymphocyte reaction.

 JoVE Clinical and Translational Medicine

Accurate and Simple Measurement of the Pro-inflammatory Cytokine IL-1β using a Whole Blood Stimulation Assay


JoVE 2662

*1, *2, 2, 1

1Translational Immunology Section, Office of Science and Technology, National Institute of Arthritis and Musculoskeletal and Skin Diseases, 2Translational Autoinflammatory Disease Section, Office of the Clinical Director, National Institute of Arthritis and Musculoskeletal and Skin Diseases

Date Published: March 1, 2011  |  doi:10.3791/2662  |  Section: JoVE Clinical and Translational Medicine

Keywords: Immunology, Interleukin-1 beta, autoinflammatory, whole blood stimulation, lipopolysaccharide, ATP, cytokine production, pattern-recognition receptors, pathogen-associated molecular patterns

 JoVE Clinical and Translational Medicine

Orthotopic Xenografting of Human Luciferase-Tagged Malignant Peripheral Nerve Sheath Tumor Cells for in vivo Testing of Candidate Therapeutic Agents


JoVE 2558

1, 1, 2, 1,3

1Department of Pathology, University of Alabama at Birmingham - UAB, 2Department of Radiology, University of Alabama at Birmingham - UAB, 3Department of Cell Biology and Neurobiology, University of Alabama at Birmingham - UAB

Date Published: March 7, 2011  |  doi:10.3791/2558  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Orthotopic grafting, Schwann cell, sciatic nerve, MPNST, neurofibrosarcoma, neurofibromatosis, experimental therapeutics

 JoVE Clinical and Translational Medicine

Thermal Ablation for the Treatment of Abdominal Tumors


JoVE 2596

*1,2, *2, *2

1Department of Biomedical Engineering, University of Wisconsin-Madison, 2Department of Radiology, University of Wisconsin-Madison

Date Published: March 7, 2011  |  doi:10.3791/2596  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Thermal ablation, interventional oncology, image-guided therapy, radiology, cancer

 JoVE Clinical and Translational Medicine

High-Efficiency Transduction of Liver Cancer Cells by Recombinant Adeno-Associated Virus Serotype 3 Vectors


JoVE 2538

, , , , , , , ,

Department of Pediatrics, Division of Cellular and Molecular Therapy, University of Florida

Date Published: March 22, 2011  |  doi:10.3791/2538  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Adeno-associated virus, viral vectors, gene transfer, gene expression, liver cancer, gene therapy

 JoVE Bioengineering

Procedure for Lung Engineering


JoVE 2651

*1, *2, 1,3

1Department of Biomedical Engineering, Yale University, 2Department of Biomedical Engineering, School of Medicine, Duke University, 3Department of Anesthesia, Yale University

Date Published: March 8, 2011  |  doi:10.3791/2651  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Decellularization, tissue engineering, lung engineering, lung tissue, extracellular matrix

 JoVE Bioengineering

A Method for Ovarian Follicle Encapsulation and Culture in a Proteolytically Degradable 3 Dimensional System


JoVE 2695

1, 2, 2,3,4, 1,4,5

1Institute for BioNanotechnology in Advanced Medicine, Northwestern University, 2Department of Obstetrics and Gynecology, Northwestern University, Feinberg School of Medicine, 3Center for Reproductive Research, Northwestern University, 4The Robert H. Lurie Comprehensive Cancer Center, Northwestern University, 5Department of Chemical and Biological Engineering, Northwestern University

Date Published: March 15, 2011  |  doi:10.3791/2695  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Ovarian follicle, fibrin-alginate, 3D culture system, dynamic environment

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