The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.

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Table of Contents

Table of Contents: Issue 65, July 2012

Jump to:  JoVE Editorial (1) JoVE General (10) JoVE Neuroscience (9) JoVE Immunology and Infection (8) JoVE Clinical and Translational Medicine (9) JoVE Bioengineering (10) JoVE Applied Physics (5)
 JoVE General

Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA


JoVE 4026

Center for Gene Regulation in Health and Disease, Department of Biological, Geological and Environmental Sciences, Cleveland State University

Date Published: July 6, 2012  |  doi:10.3791/4026  |  Section: JoVE General

 JoVE General

Conversion of a Capture ELISA to a Luminex xMAP Assay using a Multiplex Antibody Screening Method


JoVE 4084

1Chemistry Research and Development, Luminex Corporation, 2Global Marketing, Luminex Corporation

Date Published: July 6, 2012  |  doi:10.3791/4084  |  Section: JoVE General

 JoVE General

Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors


JoVE 3736

1Institute for Research in Biomedicine, Bellinzona (Switzerland), 2Dipartimento di Biologia e Genetica per le Scienze Mediche, Universitá degli Studi di Milano

Date Published: July 8, 2012  |  doi:10.3791/3736  |  Section: JoVE General

 JoVE General

Genomic Transformation of the Picoeukaryote Ostreococcus tauri


JoVE 4074

1SynthSys, University of Edinburgh, 2Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, Paris 06, 3UMR 7621, Laboratoire d'Océanographie Microbienne, Observatoire Océanologique, Banyuls-sur-Mer, Université Pierre et Marie Curie, Paris 06

Date Published: July 13, 2012  |  doi:10.3791/4074  |  Section: JoVE General

 JoVE General

RNAi-mediated Gene Knockdown and In Vivo Diuresis Assay in Adult Female Aedes aegypti Mosquitoes


JoVE 3479

1Biology Department, New Mexico State University, 2Molecular Biology Department, New Mexico State University

Date Published: July 14, 2012  |  doi:10.3791/3479  |  Section: JoVE General

 JoVE General

A Faster, High Resolution, mtPA-GFP-based Mitochondrial Fusion Assay Acquiring Kinetic Data of Multiple Cells in Parallel Using Confocal Microscopy


JoVE 3991

1Department of Neuroscience, Center for Neuroscience Research, Tufts School of Medicine, 2Department of Internal Medicine, Geriatrics & Gerontology, Wake Forest Baptist Medical Center, 3Department of Medicine, Boston University Medical Center

Date Published: July 20, 2012  |  doi:10.3791/3991  |  Section: JoVE General

 JoVE General

Creating Defined Gaseous Environments to Study the Effects of Hypoxia on C. elegans


JoVE 4088

1Department of Biochemistry, University of Washington, 2Molecular and Cellular Biology Program, University of Washington

Date Published: July 20, 2012  |  doi:10.3791/4088  |  Section: JoVE General

 JoVE General

Gene Transfer into Older Chicken Embryos by ex ovo Electroporation


JoVE 4078

1Albrecht-Kossel-Institute for Neuroregeneration, School of Medicine University of Rostock, 2Institute of Anatomy I, School of Medicine University of Jena

Date Published: July 27, 2012  |  doi:10.3791/4078  |  Section: JoVE General

 JoVE General

Synthesis of an In vivo MRI-detectable Apoptosis Probe


JoVE 3775

1Division of Cardiovascular Medicine, Department of Medicine, Stanford University Medical Center, 2Division of Cardiology, Department of Medicine, University of California, San Francisco, 3San Francisco VAMC

Date Published: July 31, 2012  |  doi:10.3791/3775  |  Section: JoVE General

 JoVE General

A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells


JoVE 4041

Department of Laboratory Medicine and Pathobiology, University of Toronto

Date Published: July 31, 2012  |  doi:10.3791/4041  |  Section: JoVE General

 JoVE Neuroscience

Functional Neuroimaging Using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI


JoVE 4055

1Department of Radiology, Stanford University, 2Center for In Vivo Microscopy, Duke University Medical Center, 3Department of Biomedical Engineering, Duke University

Date Published: July 12, 2012  |  doi:10.3791/4055  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Monitoring Changes in the Intracellular Calcium Concentration and Synaptic Efficacy in the Mollusc Aplysia


JoVE 3907

1Fishberg Department of Neuroscience and Friedman Brain Institute, Mt. Sinai School of Medicine, 2Phase Five Communications Inc.

Date Published: July 15, 2012  |  doi:10.3791/3907  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Video-oculography in Mice


JoVE 3971

1Department of Neuroscience, Erasmus MC, Rotterdam, The Netherlands, 2Department of Neuroscience, Royal Dutch Academy of Arts & Sciences (KNAW)

Date Published: July 19, 2012  |  doi:10.3791/3971  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Stereotaxic Surgery for Excitotoxic Lesion of Specific Brain Areas in the Adult Rat


JoVE 4079

1Helen Wills Neuroscience Institute, University of California Berkeley, 2Office of Laboratory Animal Care, University of California Berkeley, 3McGovern Institute for Brain Research & The Department of Brain and Cognitive Science, Massachusetts Institute of Technology, 4Integrative Biology Department, University of California Berkeley

Date Published: July 19, 2012  |  doi:10.3791/4079  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Visualization and Genetic Manipulation of Dendrites and Spines in the Mouse Cerebral Cortex and Hippocampus using In utero Electroporation


JoVE 4163

1Division of Molecular Neurobiology, MRC National Institute for Medical Research, 2Confocal and Image Analysis Laboratory, National Institute for Medical Research, 3Physiopathologie de la plasticité neuronale, Neurocentre Magendie, Université de Bordeaux

Date Published: July 26, 2012  |  doi:10.3791/4163  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Ex vivo Culturing of Whole, Developing Drosophila Brains


JoVE 4270

1National Institute of Neurological Disorders and Stroke, 2National Human Genome Research Institute, National Institutes of Health, Bethesda, MD

Date Published: July 27, 2012  |  doi:10.3791/4270  |  Section: JoVE Neuroscience

 JoVE Immunology and Infection

One-day Workflow Scheme for Bacterial Pathogen Detection and Antimicrobial Resistance Testing from Blood Cultures


JoVE 3254

1Department of Medical Microbiology, Maastricht University Medical Center, 2Department of Internal Medicine, Erasmus Medical Center

Date Published: July 9, 2012  |  doi:10.3791/3254  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

A Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.


JoVE 4177

1National Exposure Research Laboratory, Office of Research and Development, US Environmental Protection Agency, 2Shaw Environmental & Infrastructure, 3Office of Ground Water and Drinking Water, US Environmental Protection Agency

Date Published: July 9, 2012  |  doi:10.3791/4177  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors


JoVE 4029

1Division of Experimental Virology, Twincore, Centre for Experimental and Clinical Infection Research, 2Aaron Diamond AIDS Research Center, Laboratory of Retrovirology, The Rockefeller University, NY

Date Published: July 16, 2012  |  doi:10.3791/4029  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Facilitating Drug Discovery: An Automated High-content Inflammation Assay in Zebrafish


JoVE 4203

1Institute for Toxicology and Genetics, Karlsruhe Institute of Technology, Karlsruhe, Germany, 2Institute for Applied Informatics, Karlsruhe Institute of Technology, Karlsruhe, Germany

Date Published: July 16, 2012  |  doi:10.3791/4203  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Analysis of Pulmonary Dendritic Cell Maturation and Migration during Allergic Airway Inflammation


JoVE 4014

1Stem Cell and Cancer Research Institute, McMaster University, Hamilton, 2Physiology and Experimental Medicine Research Program, Hospital for Sick Children, University of Toronto

Date Published: July 23, 2012  |  doi:10.3791/4014  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation


JoVE 4097

Center for Neurologic Diseases, Brigham and Women's Hospital, Harvard Medical School

Date Published: July 23, 2012  |  doi:10.3791/4097  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Parasite Induced Genetically Driven Autoimmune Chagas Heart Disease in the Chicken Model


JoVE 3716

Chagas Disease Multidisciplinary Research Laboratory, University of Brasilia

Date Published: July 29, 2012  |  doi:10.3791/3716  |  Section: JoVE Immunology and Infection

 JoVE Clinical and Translational Medicine

Orthotopic Liver Transplantation in Rats


JoVE 4143

1Transplantation Division, Department of Surgery, University of Geneva Hospitals, 2Department of Surgery, University of Pavia, 3Department of Surgery, University of Geneva, 4Division of Abdominal Surgery, Department of Surgery, University of Geneva Hospitals

Date Published: July 1, 2012  |  doi:10.3791/4143  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Development of Obliterative Bronchiolitis in a Murine Model of Orthotopic Lung Transplantation


JoVE 3947

1Departments of Medicine, Microbiology and Immunology, Indiana University School of Medicine, 2Center for Immunobiology, Indiana University School of Medicine

Date Published: July 10, 2012  |  doi:10.3791/3947  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Isolation of Cardiomyocyte Nuclei from Post-mortem Tissue


JoVE 4205

1Strategic Research Center for Stem Cell Biology and Cell Therapy, University of Lund, 2Department of Cardiology Lund University Hospital, University of Lund

Date Published: July 10, 2012  |  doi:10.3791/4205  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

A Murine Closed-chest Model of Myocardial Ischemia and Reperfusion


JoVE 3896

1Department of Anesthesiology and Intensive Care Medicine, University of Bonn, Germany, 2Institute of Physiology, University of Bonn, Germany

Date Published: July 17, 2012  |  doi:10.3791/3896  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers


JoVE 4050

1Thayer School of Engineering, Dartmouth College, 2Department of Physics and Astronomy, Dartmouth College, 3Darmouth Medical School, Dartmouth College, 4School of Computer Science, University of Birmingham

Date Published: July 17, 2012  |  doi:10.3791/4050  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR


JoVE 3232

1Laboratorio de Investigación y Desarrollo (LID), Universidad Peruana Cayetano Heredia, 2Bloomberg School of Public Health, Johns Hopkins University, 3Laboratorio de Diagnostico Molecular, Facultad de Medicina, University of Concepcion,Chile, 4University of California San Diego School of Medicine

Date Published: July 22, 2012  |  doi:10.3791/3232  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

A Contusive Model of Unilateral Cervical Spinal Cord Injury Using the Infinite Horizon Impactor


JoVE 3313

1International Collaboration on Repair Discoveries (ICORD), University of British Columbia, 2Department of Orthopaedics, University of British Columbia

Date Published: July 24, 2012  |  doi:10.3791/3313  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

NADH Fluorescence Imaging of Isolated Biventricular Working Rabbit Hearts


JoVE 4115

1Electrical and Computer Engineering Department, The George Washington University, 2Pharmacology and Physiology Department, The George Washington University

Date Published: July 24, 2012  |  doi:10.3791/4115  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

An Orthotopic Bladder Tumor Model and the Evaluation of Intravesical saRNA Treatment


JoVE 4207

1Department of Urology and Helen Diller Comprehensive Cancer Center, University of California, San Francisco, 2Alnylam Pharmaceuticals, Inc.

Date Published: July 28, 2012  |  doi:10.3791/4207  |  Section: JoVE Clinical and Translational Medicine

 JoVE Bioengineering

High-throughput Synthesis of Carbohydrates and Functionalization of Polyanhydride Nanoparticles


JoVE 3967

1Department of Chemical and Biological Engineering, Iowa State University, 2Department of Chemistry, Iowa State University

Date Published: July 6, 2012  |  doi:10.3791/3967  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Time-lapse Fluorescence Imaging of Arabidopsis Root Growth with Rapid Manipulation of The Root Environment Using The RootChip


JoVE 4290

1Department of Plant Biology, Carnegie Institution for Science, 2Howard Hughes Medical Institute, 3Departments of Applied Physics and Bioengineering, Stanford University, 4Department of Microsystems Engineering (IMTEK) and Center for Biological Signaling Studies (BIOSS), University of Freiburg

Date Published: July 7, 2012  |  doi:10.3791/4290  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization


JoVE 3949

Departments of Chemistry and Materials Science and Engineering, University of Utah

Date Published: July 11, 2012  |  doi:10.3791/3949  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Solubilization and Bio-conjugation of Quantum Dots and Bacterial Toxicity Assays by Growth Curve and Plate Count


JoVE 3969

Department of Biomedical Engineering, McGill University, Montreal, QC Canada

Date Published: July 11, 2012  |  doi:10.3791/3969  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Continuously-stirred Anaerobic Digester to Convert Organic Wastes into Biogas: System Setup and Basic Operation


JoVE 3978

Department of Biological and Environmental Engineering, Cornell University

Date Published: July 13, 2012  |  doi:10.3791/3978  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Candida albicans Biofilm Chip (CaBChip) for High-throughput Antifungal Drug Screening


JoVE 3845

1Department of Biomedical Engineering, University of Texas at San Antonio, 2Department of Biology, University of Texas at San Antonio

Date Published: July 18, 2012  |  doi:10.3791/3845  |  Section: JoVE Bioengineering

 JoVE Bioengineering

On-Chip Endothelial Inflammatory Phenotyping


JoVE 4169

Department of Biomedical Engineering, University of California, Davis

Date Published: July 21, 2012  |  doi:10.3791/4169  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Three-dimensional Cell Culture Model for Measuring the Effects of Interstitial Fluid Flow on Tumor Cell Invasion


JoVE 4159

School of Biomedical Engineering, Science and Health Systems, Drexel University

Date Published: July 25, 2012  |  doi:10.3791/4159  |  Section: JoVE Bioengineering

 JoVE Applied Physics

Fabrication, Densification, and Replica Molding of 3D Carbon Nanotube Microstructures


JoVE 3980

1Mechanosynthesis Group, Department of Mechanical Engineering, University of Michigan, 2IMEC, Belgium

Date Published: July 2, 2012  |  doi:10.3791/3980  |  Section: JoVE Applied Physics

 JoVE Applied Physics

Fabrication of Silica Ultra High Quality Factor Microresonators


JoVE 4164

1Department of Chemical Engineering and Materials Science, University of Southern California, 2Department of Electrical Engineering-Electrophysics, University of Southern California

Date Published: July 2, 2012  |  doi:10.3791/4164  |  Section: JoVE Applied Physics

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