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The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.

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Table of Contents

Table of Contents: Issue 48, February 2011

Jump to:  JoVE General (11) JoVE Neuroscience (15) JoVE Immunology and Infection (9) JoVE Clinical and Translational Medicine (11) JoVE Bioengineering (5)
 JoVE General

Isolation of Mouse Salivary Gland Stem Cells


JoVE 2484

1,2, 1, 1, 1,2, 1,2

1Department of Cell Biology, University Medical Center Groningen, University of Groningen, 2Department of Radiation Oncology, University Medical Center Groningen, University of Groningen

Date Published: February 8, 2011  |  doi:10.3791/2484  |  Section: JoVE General

Keywords: Stem Cell Biology, Murine salivary glands, stem cells, isolation, tissue culture.

 JoVE General

Identifying the Effects of BRCA1 Mutations on Homologous Recombination using Cells that Express Endogenous Wild-type BRCA1


JoVE 2468

1, 2, 1

1Department of Biomedical Informatics, The Ohio State University, 2Departments of Molecular Immunology and Clinical Oncology, Tohoku University

Date Published: February 17, 2011  |  doi:10.3791/2468  |  Section: JoVE General

Keywords: Cell Biology, BRCA1, homologous recombination, breast cancer, RNA interference, DNA repair

 JoVE General

Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE) for Analysis of Multiprotein Complexes from Cellular Lysates


JoVE 2164

1,2,3, *2,3, *3

1Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, 2Centre for Biological Signalling Studies (bioss) and Biology III, Faculty of Biology, University of Freiburg, 3Department of Molecular Immunology, Max-Planck-Institute of Immunology and Epigenetics

Date Published: February 24, 2011  |  doi:10.3791/2164  |  Section: JoVE General

Keywords: Biochemistry, BN-PAGE, 2D gel electrophoresis, cellular lysate, dialysis, protein complex, multiprotein complex, protein interaction

 JoVE General

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses


JoVE 2498

1, 1, 2, 1

1Department of Biological Sciences, University of Alabama Huntsville, 2Department of Biology, Stanford University

Date Published: February 25, 2011  |  doi:10.3791/2498  |  Section: JoVE General

Keywords: Molecular Biology, Ribosome stalling, ribosome isolation, peptidyl-tRNA, in vitro translation, RNA chemical modification, puromycin, antibiotics.

 JoVE General

Stable Isotopic Profiling of Intermediary Metabolic Flux in Developing and Adult Stage Caenorhabditis elegans


JoVE 2288

1,2, *1, *1, 1, 1, 1,2

1Department of Pediatrics, The Children's Hospital of Philadelphia, 2Department of Pediatrics, University of Pennsylvania

Date Published: February 27, 2011  |  doi:10.3791/2288  |  Section: JoVE General

Keywords: Developmental Biology, Stable isotope, amino acid quantitation, organic acid quantitation, nematodes, metabolism

 JoVE General

Respirometric Oxidative Phosphorylation Assessment in Saponin-permeabilized Cardiac Fibers


JoVE 2431

1, 1,2, 2, 1,2

1Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Calgary, 2Faculty of Kinesiology, University of Calgary

Date Published: February 28, 2011  |  doi:10.3791/2431  |  Section: JoVE General

Keywords: Physiology, cardiac fibers, mitochondria, oxygen consumption, mouse, methodology

 JoVE Neuroscience

Focal Cerebral Ischemia Model by Endovascular Suture Occlusion of the Middle Cerebral Artery in the Rat


JoVE 1978

, , , ,

Department of Neurological Surgery, School of Medicine and Public Health, University of Wisconsin-Madison

Date Published: February 5, 2011  |  doi:10.3791/1978  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Stroke, cerebral ischemia, middle cerebral artery occlusion, intraluminal filament, rat, magnetic resonance imaging, surgery, neuroscience, brain

 JoVE Neuroscience

Automated Interactive Video Playback for Studies of Animal Communication


JoVE 2374

1, 1, 2, 2, 2, 2

1Department of Visualization, Texas A&M University (TAMU), 2Department of Biology, Texas A&M University (TAMU)

Date Published: February 9, 2011  |  doi:10.3791/2374  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Computer animation, visual communication, mate choice, Xiphophorus birchmanni, tracking

 JoVE Neuroscience

Postsynaptic Recordings at Afferent Dendrites Contacting Cochlear Inner Hair Cells: Monitoring Multivesicular Release at a Ribbon Synapse


JoVE 2442

*1, *1, 2, 1

1Department of Otolaryngology-Head and Neck Surgery., The Johns Hopkins School of Medicine, 2Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Consejo Nacional de Investigaciones Científicas y Técnicas

Date Published: February 10, 2011  |  doi:10.3791/2442  |  Section: JoVE Neuroscience

Keywords: Neuroscience, electrophysiology, whole-cell recording, patch clamp, synaptic transmission, ribbon synapse, multivesicular, dendrite, auditory nerve, hearing, hair cell.

 JoVE Neuroscience

The Culture of Primary Motor and Sensory Neurons in Defined Media on Electrospun Poly-L-lactide Nanofiber Scaffolds


JoVE 2389

1, 2, 3, 3, 3, 3, 3, 1,3,4

1Department of Biomedical Engineering, University of Michigan, 2State Key Laboratory of Bioelectronics, Southeast University, 3Department of Neurology, University of Michigan, 4Geriatric Research, Education and Clinical Center, Veterans Affairs Ann Arbor Health System

Date Published: February 15, 2011  |  doi:10.3791/2389  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Electrospinning, motor neurons, nanofibers, scaffold, primary cell culture, DRG

 JoVE Neuroscience

Slice Preparation, Organotypic Tissue Culturing and Luciferase Recording of Clock Gene Activity in the Suprachiasmatic Nucleus


JoVE 2439

*, *, ,

Swedish Medical Nanoscience Center, Department of Neuroscience, Karolinska Institutet

Date Published: February 15, 2011  |  doi:10.3791/2439  |  Section: JoVE Neuroscience

Keywords: Neuroscience, suprachiasmatic nucleus, mice, organotypic tissue culture, circadian rhythm, clock gene, Period 2, luciferase

 JoVE Neuroscience

Kinematics and Ground Reaction Force Determination: A Demonstration Quantifying Locomotor Abilities of Young Adult, Middle-aged, and Geriatric Rats


JoVE 2138

1,2, 3, 3, 4, 3

1CullenWebb Animal Neurology & Ophthalmology Center, Riverview, NB, 2Department of Clinical Neurosciences, Faculty of Medicine, University of Calgary, 3Department of Comparative Biology and Experimental Medicine, University of Calgary, 4Department of Neuroscience, University of Calgary

Date Published: February 22, 2011  |  doi:10.3791/2138  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Locomotion, kinetics, kinematics, aging

 JoVE Neuroscience

Assessment of Social Interaction Behaviors


JoVE 2473

1, 1, 2, 1,3,4,5, 1,3

1Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 2Toronto Centre for Phenogenomics, Mount Sinai Hospital, 3Department of Medical Biophysics, University of Toronto, 4Department of Psychology, University of Toronto, 5Department of Psychiatry, University of Toronto

Date Published: February 25, 2011  |  doi:10.3791/2473  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Mice, behavioral test, phenotyping, social interaction

 JoVE Neuroscience

Multiple-mouse Neuroanatomical Magnetic Resonance Imaging


JoVE 2497

*1, *1, 1, 1,2

1Mouse Imaging Centre, Hospital for Sick Children, 2Department of Medical Biophysics and Medical Imaging, University of Toronto

Date Published: February 27, 2011  |  doi:10.3791/2497  |  Section: JoVE Neuroscience

Keywords: Neuroscience, magnetic resonance imaging, mouse, phenotyping, mouse handling, monitoring, brain, multiple mouse imaging

 JoVE Immunology and Infection

A 1.5 Hour Procedure for Identification of Enterococcus Species Directly from Blood Cultures


JoVE 2616

1, 2, 2, 2, 3, 3, 4

1Department of Pathology and Laboratory Medicine, Cedars-Sinai Medical Cente, 2Pasadena, CA, Southern California Permanente Medical Group, 3Detroit, Detroit Medical Center, 4Woburn, MA, AdvanDx

Date Published: February 10, 2011  |  doi:10.3791/2616  |  Section: JoVE Immunology and Infection

Keywords: Immunology, PNA FISH, Enterococcus, Blood Culture, Sepsis, Staining

 JoVE Immunology and Infection

Methods for Rapid Transfer and Localization of Lyme Disease Pathogens Within the Tick Gut


JoVE 2544

1, 1, 2, 1

1Department of Veterinary Medicine, University of Maryland, 2Department of Entomology, Connecticut Agricultural Experiment Station

Date Published: February 14, 2011  |  doi:10.3791/2544  |  Section: JoVE Immunology and Infection

Keywords: Infection, Lyme disease, tick, microinjection, Borrelia burgdorferi, immunofluorescence microscopy

 JoVE Immunology and Infection

Finger-stick Blood Sampling Methodology for the Determination of Exercise-induced Lymphocyte Apoptosis


JoVE 2595

1, *2, 2, 1, 1, 1, 1, 1

1Department of Kinesiology, Recreation, and Sport, Western Kentucky University, 2Department of Health and Human Performance, University of Houston

Date Published: February 24, 2011  |  doi:10.3791/2595  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Leukocyte phenotyping, programmed cell death, muscular activity, technique development

 JoVE Clinical and Translational Medicine

Hyperinsulinemic-Euglycemic Clamp in the Conscious Rat


JoVE 2432

1, 1,2, 2, 1,2

1Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Calgary, 2Faculty of Kinesiology, University of Calgary

Date Published: February 7, 2011  |  doi:10.3791/2432  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Metabolism, Diabetes, Insulin Sensitivity, Methodology

 JoVE Clinical and Translational Medicine

Magnetic Resonance Derived Myocardial Strain Assessment Using Feature Tracking


JoVE 2356

1, 2, 3, 3, 1, 1, 1, 4

1The Heart Institute, Cincinnati Children Hospital Medical Center (CCHMC), 2TomTec, Imaging Systems GmbH, 3AMID, Advanced Medical Imaging Development SRL, 4The Heart and Vascular Center, The Christ Hospital

Date Published: February 12, 2011  |  doi:10.3791/2356  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, feature tracking, strain, displacement, CMR

 JoVE Clinical and Translational Medicine

Using Visual and Narrative Methods to Achieve Fair Process in Clinical Care


JoVE 2342

*1,2, *2

1Institute for Behavioral Health, Brandeis University, 2Heller School for Social Policy and Management, Brandeis University

Date Published: February 16, 2011  |  doi:10.3791/2342  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, person-centered care, participatory visual methods, photovoice, photo-elicitation, narrative medicine, acquired brain injury, disability, rehabilitation, palliative care

 JoVE Clinical and Translational Medicine

Acute Myocardial Infarction in Rats


JoVE 2464

1, 2, 2, 1, 2

1Department of Internal Medicine, Division of Cardiology, University of Texas Medical Branch, 2Department of Pharmacological and Pharmaceutical Sciences, College of Pharmacy, University of Houston (UH), Texas Medical Center

Date Published: February 16, 2011  |  doi:10.3791/2464  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Cardiovascular (CV), Heart Failure (HF), Acute Myocardial Infarction (AMI), Ischemia-Reperfusion (IR), Left Anterior Descending Artery (LAD)

 JoVE Clinical and Translational Medicine

Isolation of Mouse Respiratory Epithelial Cells and Exposure to Experimental Cigarette Smoke at Air Liquid Interface


JoVE 2513

1,2, 1, 1

1Department of Medicine, Pulmonary and Critical Care Medicine, Brigham and Women’s Hospital, Harvard Medical School, 2Cellular and Molecular Pathology, School of Medicine, University of Pittsburgh

Date Published: February 21, 2011  |  doi:10.3791/2513  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Air-Liquid Interface, Cell isolation, Cigarette smoke, Epithelial cells

 JoVE Clinical and Translational Medicine

A Protocol for Comprehensive Assessment of Bulbar Dysfunction in Amyotrophic Lateral Sclerosis (ALS)


JoVE 2422

1,2, 3, 3, 4, 2,5

1Department of Speech-Language Pathology, University of Toronto, 2ALS/ MN Clinic, Sunnybrook Health Science Centre, 3Department of Special Education and Communication Disorders, University of Nebraska-Lincoln, 4Department of Neurology, Munroe-Meyer Institute, University of Nebraska Medical Center, 5Department of Neurology, University of Toronto

Date Published: February 21, 2011  |  doi:10.3791/2422  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, speech, assessment, subsystems, bulbar function, amyotrophic lateral sclerosis

 JoVE Clinical and Translational Medicine

Myo-mechanical Analysis of Isolated Skeletal Muscle


JoVE 2582

1,2, 3, 2, 4, 1,2,5

1Cardiovascular Research Institute, University of California San Francisco, 2Department of Pediatrics, University of California San Francisco, 3Department of Biology, San Francisco State University, 4Department of Medicine, University of California San Francisco, 5Eli and Edythe Broad Center of Regeneration Medicine & Stem Cell Research, University of California San Francisco

Date Published: February 22, 2011  |  doi:10.3791/2582  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, muscle, twitch, tetanus, force-frequency, fatigue

 JoVE Clinical and Translational Medicine

FISH for Pre-implantation Genetic Diagnosis


JoVE 2570

, ,

Department of Cytogenetics, GSTS-Pathology, Guy’s & St Thomas’ NHS Foundation Trust, Guy’s & St Thomas’ Centre for Preimplantation Genetic Diagnosis

Date Published: February 23, 2011  |  doi:10.3791/2570  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Fluorescence in situ hybridization, Pre-implantation genetic diagnosis, PGD, Sex determination, Translocations, Chromosome aneuploidy

 JoVE Bioengineering

Developing Custom Chinese Hamster Ovary-host Cell Protein Assays using Acoustic Membrane Microparticle Technology


JoVE 2493

1, 2, 2, 1

1Biomarker Division, BioScale, Inc., 2Bioprocessing Division, BioScale, Inc.

Date Published: February 3, 2011  |  doi:10.3791/2493  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Immunoassays, Chinese Hamster Ovary Host Cell Protein, Residual Protein A assay, Assay development, Biomarker detection and quantitation, Phospho-AKT, Gadd34, tissue sample, tumor sample, bioreactor sample

 JoVE Bioengineering

Decellularization and Recellularization of Whole Livers


JoVE 2394

, , , , , ,

Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School, Shriners Hospitals for Children

Date Published: February 4, 2011  |  doi:10.3791/2394  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Liver extracellular matrix, decellularization, recellularization, hepatocytes, bioreactor

 JoVE Bioengineering

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow


JoVE 2545

1,2, 1,2, 1,2, 2,3, 1,2,4

1Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, 2Micro and Nanotechnology Lab, University of Illinois at Urbana-Champaign, 3Mechanical Science and Engineering, University of Illinois at Urbana-Champaign, 4Bioengineering, University of Illinois at Urbana-Champaign

Date Published: February 4, 2011  |  doi:10.3791/2545  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Dielectrophoresis, microfluidic, laminar flow, cell sorting, Human colon adenocarcinoma

 JoVE Bioengineering

Studying Cell Rolling Trajectories on Asymmetric Receptor Patterns


JoVE 2640

1, 2, 1, 3, 2

1Department of Materials Science and Engineering, MIT - Massachusetts Institute of Technology, 2Department of Mechanical Engineering, MIT - Massachusetts Institute of Technology, 3HST Center for Biomedical Engineering and Harvard Stem Cell Institute, Brigham and Women's Hospital and Harvard Medical School

Date Published: February 13, 2011  |  doi:10.3791/2640  |  Section: JoVE Bioengineering

Keywords: Bioengineering, cell rolling, microcontact printing, cell adhesion, cell analysis, cell separation, P-selectin

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