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The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.

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Table of Contents

Table of Contents: Issue 39, May 2010

Jump to:  JoVE General (16) JoVE Neuroscience (8) JoVE Immunology and Infection (1) JoVE Clinical and Translational Medicine (3)
 JoVE General

Deciphering Axonal Pathways of Genetically Defined Groups of Neurons in the Chick Neural Tube Utilizing in ovo Electroporation


JoVE 1792

*, *, , ,

Department of Medical Neurobiology, Institute for Medical Research Israel Canada, Hebrew University-Hadassah Medical School

Date Published: May 2, 2010  |  doi:10.3791/1792  |  Section: JoVE General

Keywords: Neuroscience, in ovo electroporation, neural tube, chick, interneurons, axonal pathway

 JoVE General

Window on a Microworld: Simple Microfluidic Systems for Studying Microbial Transport in Porous Media


JoVE 1741

1,2, 1, 1, 1, 1,2,3,4, 5,6

1Vanderbilt Institute for Integrative Biosystems Research and Education, Vanderbilt University, 2Department of Biomedical Engineering, Vanderbilt University, 3Department of Molecular Physiology and Biophysics, Vanderbilt University, 4Department of Physics and Astronomy, Vanderbilt University, 5Department of Chemical, Materials and Biomolecular Engineering, University of Connecticut, 6Center for Environmental Sciences and Engineering, University of Connecticut

Date Published: May 3, 2010  |  doi:10.3791/1741  |  Section: JoVE General

Keywords: Microbiology, Microfluidic device, bacterial transport, porous media, colloid, biofilm, filtration theory, artificial habitat, micromodel, PDMS, GFP

 JoVE General

Robotics and Dynamic Image Analysis for Studies of Gene Expression in Plant Tissues


JoVE 1733

1, 1,2, 1

1Department of Horticulture and Crop Science, The Ohio State University, 2Department of Plant Pathology, North Carolina State University

Date Published: May 5, 2010  |  doi:10.3791/1733  |  Section: JoVE General

Keywords: Plant Biology, gfp, image analysis, time-lapse animation, transient expression, promoters, transgene expression, transformation

 JoVE General

Electron Cryotomography of Bacterial Cells


JoVE 1943

1, 1,2, 1, 1,2, 1,2, 2, 1, 1,2, 1,2, 1, 1,2, 1, 1, 1,2

1Division of Biology, California Institute of Technology - Caltech, 2Howard Hughes Medical Institute, California Institute of Technology - Caltech

Date Published: May 6, 2010  |  doi:10.3791/1943  |  Section: JoVE General

Keywords: Cellular Biology, Electron cryotomography, microbiology, bacteria, electron microscopy

 JoVE General

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.


JoVE 1869

*1, *2,3,4,5, *2, 6, 2, 3,6, 1, 2,7,8

1Program in Gene Function and Expression, Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 2Broad Institute of Harvard and Massachusetts Institute of Technology, 3Division of Health Sciences and Technology, Massachusetts Institute of Technology, 4Program for Evolutionary Dynamics, Department of Organismic and Evolutionary Biology, Department of Mathematics, Harvard University, 5Department of Applied Mathematics, Harvard University, 6Department of Physics, Massachusetts Institute of Technology, 7Department of Systems Biology, Harvard Medical School, 8Department of Biology, Massachusetts Institute of Technology

Date Published: May 6, 2010  |  doi:10.3791/1869  |  Section: JoVE General

Keywords: Cellular Biology, Chromosome conformation capture, chromatin structure, Illumina Paired End sequencing, polymer physics.

 JoVE General

Video Bioinformatics Analysis of Human Embryonic Stem Cell Colony Growth


JoVE 1933

1,2,3, 1, 1,3, 4, 1,2

1UCR Stem Cell Center, University of California, 2Department of Cell Biology and Neuroscience, University of California, 3Cell, Molecular, and Developmental Biology Graduate Program, University of California, 4Center for Research in Intelligent Systems, University of California

Date Published: May 20, 2010  |  doi:10.3791/1933  |  Section: JoVE General

Keywords: Cellular Biology, hESC, matrigel, stem cells, video bioinformatics, colony, growth

 JoVE General

Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana


JoVE 1925

1, 1,2

1Department of Energy - Plant Research Laboratory, Michigan State University (MSU), 2Department of Biochemistry and Molecular Biology, Michigan State University (MSU)

Date Published: May 29, 2010  |  doi:10.3791/1925  |  Section: JoVE General

Keywords: Plant Biology, Arabidopsis thaliana, confocal microscopy, expression profiling, microarray, fluorescence, FACS, photomorphogenesis, phytochrome, protoplasting

 JoVE General

How to Culture, Record and Stimulate Neuronal Networks on Micro-electrode Arrays (MEAs)


JoVE 2056

1,2, 2,3, 2

1Department of Neurology, Emory University School of Medicine, 2Coulter Department of Biomedical Engineering, Laboratory for Neuroengineering, Georgia Institute of Technology and Emory, University School of Medicine, 3Emory University School of Medicine

Date Published: May 30, 2010  |  doi:10.3791/2056  |  Section: JoVE General

Keywords: Neuroscience, micro-electrode, multi-electrode, neural, MEA, network, plasticity, spike, stimulation, recording, rat

 JoVE Neuroscience

The Subventricular Zone En-face: Wholemount Staining and Ependymal Flow


JoVE 1938

1, 2,3, 4, 2,5, 1

1Department of Neurosurgery, The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco - UCSF, 2Department of Pathology and Cell Biology, College of Physicians and Surgeons, Columbia University, 3Department of Neuroscience and Neurology, College of Physicians and Surgeons, Columbia University, 4Department of Developmental and Regenerative Biology, Nagoya City University Graduate School of Medical Sciences, 5Center for Motor Neuron Biology and Disease, College of Physicians and Surgeons, Columbia University

Date Published: May 6, 2010  |  doi:10.3791/1938  |  Section: JoVE Neuroscience

Keywords: JoVE Neuroscience, brain, neurogenesis, neural stem cell, ependymal cell, ependymal flow, planar cell polarity, wholemount, pinwheel

 JoVE Neuroscience

Selection of Aptamers for Amyloid β-Protein, the Causative Agent of Alzheimer's Disease


JoVE 1955

1, 1,2,3

1Department of Neurology, David Geffen School of Medicine, 2Molecular Biology Institute, University of California, Los Angeles, 3Brain Research Institute, University of California, Los Angeles

Date Published: May 13, 2010  |  doi:10.3791/1955  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Cellular Biology, Aptamer, RNA, amyloid β-protein, oligomer, amyloid fibrils, protein assembly

 JoVE Neuroscience

A Simple Composite Phenotype Scoring System for Evaluating Mouse Models of Cerebellar Ataxia


JoVE 1787

1, 2, 1, 2, *3, *2

1Department of Biochemistry, University of Washington, 2Department of Neurology, University of Washington, 3Division of Genetics, Departments of Pediatrics and Cellular and Molecular Medicine, and the Institute for Genomic Medicine, University of California, San Diego - Rady Children’s Hospital

Date Published: May 21, 2010  |  doi:10.3791/1787  |  Section: JoVE Neuroscience

Keywords: JoVE Neuroscience, Neurodegeneration, Mouse behavior assay, cerebellar ataxia, polyglutamine disease

 JoVE Neuroscience

Low-stress Route Learning Using the Lashley III Maze in Mice


JoVE 1786

1, 2, 3,4,5,6

1Department of Chemistry, Pennsylvania State University, 2Center for Developmental and Health Genetics, Pennsylvania State University, 3Department of Veterinary and Biomedical Sciences, Pennsylvania State University, 4Huck Institute of the Life Sciences, Pennsylvania State University, 5California NanoSystems Institute, University of California, Los Angeles, 6Semel Institute of Neuroscience and Human Behavior, University of California, Los Angeles

Date Published: May 22, 2010  |  doi:10.3791/1786  |  Section: JoVE Neuroscience

Keywords: Neuroscience, mouse, behavior testing, learning, memory, neuroscience, phenotyping, aging

 JoVE Neuroscience

Laser Capture Microdissection of Drosophila Peripheral Neurons


JoVE 2016

1,2, 1,2

1Department of Molecular and Microbiology, George Mason University, 2Krasnow Institute for Advanced Study, George Mason University

Date Published: May 24, 2010  |  doi:10.3791/2016  |  Section: JoVE Neuroscience

Keywords: JoVE Neuroscience, Drosophila, neuron, Peripheral Nervous System (PNS), dendritic arborization neurons, RNA, microarray, qRT-PCR, cell isolation, Laser Capture Microdissection (LCM)

 JoVE Neuroscience

Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord


JoVE 1773

*1,2, *1, 1,3,4, 1,2,5,6,7

1Molecular Biology of Neural Development, Institut de Recherches Cliniques de Montréal, 2Division of Experimental Medicine and Program in Neuroengineering, McGill University, 3Program in Neuroengineering, McGill University, 4Montreal Neurological Institute, 5Department of Anatomy and Cell Biology, McGill University, 6Department of Biology, McGill University, 7Department of Medicine, Universite de Montreal - University of Montreal

Date Published: May 25, 2010  |  doi:10.3791/1773  |  Section: JoVE Neuroscience

Keywords: JoVE Neuroscience, Development, Axon Guidance, Spinal cord, Dissection, Primary Neuron Culture, Commissural Neurons

 JoVE Immunology and Infection

Biocontained Carcass Composting for Control of Infectious Disease Outbreak in Livestock


JoVE 1946

1, 2, 1, 1, 1, 1, 3, 1

1Agriculture and Agri-Food Canada, Lethbridge Research Centre, 2Department of Bioscience and Biotechnology, Dalian University of Technology, 3Agriculture Centre, Alberta Agriculture and Rural Development

Date Published: May 6, 2010  |  doi:10.3791/1946  |  Section: JoVE Immunology and Infection

Keywords: JoVE Infectious Diseases, compost, livestock, infectious disease, biocontainment

 JoVE Clinical and Translational Medicine

A Novel Capsulorhexis Technique Using Shearing Forces with Cystotome


JoVE 1962

1, 2, 3

1Department of Ophthalmology, Hairmyres Hospital, NHS Lanarkshire, 2Royal Devon and Exeter NHS Foundation Trust, Department of Ophthalmology, 3Department of Ophthalmology, South Devon Healthcare NHS Trust

Date Published: May 15, 2010  |  doi:10.3791/1962  |  Section: JoVE Clinical and Translational Medicine

Keywords: JoVE Medicine, Phacoemulsification surgery, cataract surgery, capsulorhexis, capsulotomy, technique, Continuous curvilinear capsulorhexis, cystotome

 JoVE Clinical and Translational Medicine

Programmed Electrical Stimulation in Mice


JoVE 1730

1, 1,2

1Department of Molecular Physiology and Biophysics, Baylor College of Medicine (BCM), 2The Margaret M. and Albert B. Alkek Department of Medicine, Baylor College of Medicine (BCM)

Date Published: May 26, 2010  |  doi:10.3791/1730  |  Section: JoVE Clinical and Translational Medicine

Keywords: JoVE Medicine, Arrhythmias, electrophysiology, mouse, programmed electrical stimulation

 JoVE Clinical and Translational Medicine

Transthoracic Echocardiography in Mice


JoVE 1738

1, 1,2

1Department of Molecular Physiology and Biophysics, Baylor College of Medicine (BCM), 2The Margaret M. and Albert B. Alkek Department of Medicine, Baylor College of Medicine (BCM)

Date Published: May 28, 2010  |  doi:10.3791/1738  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Echocardiography, pulse wave Doppler, tissue Doppler imaging, ultrasound

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