Table of Contents: Issue 34, December 2009

The 2009 Lindau Nobel Laureate Meeting: Peter Agre, Chemistry 2003

JoVE 1565

Peter Agre

Date Published: December 9, 2009  |  doi:10.3791/1565  |  Section: JoVE Editorial

Keywords: Cellular Biology, aquaporins, water-channel, diffusion, renal tubules, red blood cells

Isolation and Purification of Drosophila Peripheral Neurons by Magnetic Bead Sorting

JoVE 1599

Eswar Prasad R. Iyer^1,2, Srividya Chandramouli Iyer^1,2, Mikolaj J. Sulkowski^1,2, Daniel N. Cox^1,2

¹Department of Molecular and Microbiology, George Mason University, ²Krasnow Institute for Advanced Study, George Mason University

Date Published: December 1, 2009  |  doi:10.3791/1599  |  Section: JoVE General

Keywords: Cellular Biology, Drosophila, cell dissociation, neuron, Peripheral Nervous System (PNS), dendritic arborization neurons, RNA, microarray, magnetic bead sorting, cell isolation

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA

JoVE 1622

Katherine H. Watkins¹, Allan Stewart¹, William G. Fairbrother^1,2

¹Department of Molecular and Cellular Biology, Brown University, ²Center for Computational Molecular Biology, Brown University

Date Published: December 2, 2009  |  doi:10.3791/1622  |  Section: JoVE General

Keywords: Cellular Biology, pre-mRNA, splicing factors, tiling array, ribonucleoprotein (RNP), binding maps

Using the GELFREE 8100 Fractionation System for Molecular Weight-Based Fractionation with Liquid Phase Recovery

JoVE 1842

Chuck Witkowski, Jay Harkins

Research and Development, Protein Discovery, Inc.

Date Published: December 3, 2009  |  doi:10.3791/1842  |  Section: JoVE General

Keywords: Basic Protocols, Cellular Biology, GELFREE, SDS PAGE, gel electrophoresis, protein fractionation, separation, electrophoresis, proteomics, mass spectrometry

Optical Recording of Electrical Activity in Guinea-pig Enteric Networks using Voltage-sensitive Dyes

JoVE 1631

Ana L. Obaid¹, B. M. Salzberg^1,2

¹Department of Neuroscience, University of Pennsylvania-School of Medicine, ²Department of Physiology, University of Pennsylvania-School of Medicine

Date Published: December 4, 2009  |  doi:10.3791/1631  |  Section: JoVE General

Keywords: Cellular Biology, naphthylstyryl-pyridinium dye, di-4-ANEPPDHQ, enteric nervous system, submucous plexus, myenteric plexus, potentiometric probes, enteric plexuses, neural networks, gut, optical recording, voltage-sensitive dyes

Retro-orbital Injection in Adult Zebrafish

JoVE 1645

Emily K. Pugach^1,2, Pulin Li^1,2, Richard White^1,2,3, Leonard Zon^1,2

¹Department of Hematology and Oncology, Children’s Hospital Boston, ²Harvard Stem Cell Institute, Harvard Medical School, ³Department of Medical Oncology, Dana Farber Cancer Institute

Date Published: December 7, 2009  |  doi:10.3791/1645  |  Section: JoVE General

Keywords: Cellular Biology, fluorescent dye, kidney marrow cells, vasculature, red blood cells, Zebrafish, injection, retro-orbital injection, transplantation, HSC

Generation of Induced Pluripotent Stem Cells by Reprogramming Human Fibroblasts with the Stemgent Human TF Lentivirus Set

JoVE 1553

Dongmei Wu, Brad Hamilton, Charles Martin, Yan Gao, Mike Ye, Shuyuan Yao

Research and Development, Stemgent

Date Published: December 8, 2009  |  doi:10.3791/1553  |  Section: JoVE General

Keywords: Developmental Biology, iPS, reprogramming, lentivirus, stem cell, induced pluripotent cell, pluripotency, fibroblast, embryonic stem cells, ES cells, iPS cells

Intranuclear Microinjection of DNA into Dissociated Adult Mammalian Neurons

JoVE 1614

Van B. Lu, Damian J. Williams, Yu-Jin Won, Stephen R. Ikeda

Laboratory of Molecular Physiology, National Institute on Alcohol Abuse and Alcoholism (NIAAA), National Institutes of Health (NIH)

Date Published: December 10, 2009  |  doi:10.3791/1614  |  Section: JoVE General

Keywords: Cellular Biology, Neuroscience, Molecular biology, Heterologous expression, Nuclear transfection, DNA injection, microinjection, SGC neurons, GIRK channels, neurons

Osmotic Avoidance in Caenorhabditis elegans: Synaptic Function of Two Genes, Orthologues of Human NRXN1 and NLGN1, as Candidates for Autism

JoVE 1616

Fernando Calahorro^1,2, Encarna Alejandre^1,2, Manuel Ruiz-Rubio^1,2

¹Departamento de Genética, Facultad de Ciencias, Universidad de Córdoba, ²Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC)

Date Published: December 11, 2009  |  doi:10.3791/1616  |  Section: JoVE General

Keywords: Neuroscience, Microbiology, synapse, osmotic sensitivity, Caenorhabditis elegans, neurexin, neuroligin, autism, neuroscience

Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics

JoVE 1650

Onur Mudanyali¹, Anthony Erlinger¹, Sungkyu Seo¹, Ting-Wei Su¹, Derek Tseng¹, Aydogan Ozcan^1,2

¹Electrical Engineering Department, University of California, Los Angeles, ²California NanoSystems Institute, University of California, Los Angeles

Date Published: December 14, 2009  |  doi:10.3791/1650  |  Section: JoVE General

Keywords: Cellular Biology, LUCAS, lensfree imaging, on-chip imaging, point-of-care diagnostics, global health, cell-biology, telemedicine, wireless health, microscopy, red blood cells

A Novel RFP Reporter to Aid in the Visualization of the Eye Imaginal Disc in Drosophila

JoVE 1617

Aamna K. Kaul, Joseph M. Bateman

Wolfson Centre for Age-Related Diseases, King's College London

Date Published: December 15, 2009  |  doi:10.3791/1617  |  Section: JoVE General

Keywords: Cellular Biology, fluorescence microscopy, Drosophila, eye, RFP, dissection, imaginal disc

Extraction of the EPP Component from the Surface EMG

JoVE 1653

Toshifumi Kumai

Graduate School of Oral Medicine, Matsumoto Dental University

Date Published: December 16, 2009  |  doi:10.3791/1653  |  Section: JoVE General

Keywords: Neuroscience, masseter muscle, EMG, EPP, neuromuscular junction, EPP oscillation

PuraMatrix Encapsulation of Cancer Cells

JoVE 1692

Adnan O. Abu-Yousif¹, Imran Rizvi^1,2, Conor L. Evans¹, Jonathan P. Celli¹, Tayyaba Hasan^1,3

¹Wellman Center for Photomedicine Massachusetts General Hospital, Harvard Medical School, ²Thayer School of Engineering, Dartmouth College, ³Department of Dermatology, Harvard Medical School

Date Published: December 17, 2009  |  doi:10.3791/1692  |  Section: JoVE General

Keywords: Cellular Biology, PuraMatrix, OVCAR-5 cells, Cancer, in vitro models, 3D, Encapsulation, PuraMatrix,

Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube

JoVE 1630

Nadine H. Soplop^1,2, Rajesh Patel¹, Sunita G. Kramer^1,2

¹Joint Graduate Program in Cell and Developmental Biology, UMDNJ-Graduate School of Biomedical Sciences and Rutgers: The State University of New Jersey, ²Department of Pathology and Laboratory Medicine, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey

Date Published: December 21, 2009  |  doi:10.3791/1630  |  Section: JoVE General

Keywords: Developmental Biology, Drosophila, transmission electron microscopy, fixation, sectioning, embryonic heart tube, lumen

Culture and Maintenance of Human Embryonic Stem Cells

JoVE 1427

Lia Kent

Research and Development, Stemgent

Date Published: December 22, 2009  |  doi:10.3791/1427  |  Section: JoVE General

Keywords: Developmental Biology, Human embryonic stem cell, medium, hES, maintenance, thaw, ES cells, stem cell, cell culture, pluripotency, differentiation, passage, freeze

Measuring Plasma Membrane Protein Endocytic Rates by Reversible Biotinylation

JoVE 1669

Luke Gabriel, Zachary Stevens, Haley Melikian

University of Massachusetts Medical School

Date Published: December 23, 2009  |  doi:10.3791/1669  |  Section: JoVE General

Keywords: Cellular Biology, Cell biology, membrane trafficking, endocytosis, biotinylation

Freezing and Thawing Human Embryonic Stem Cells

JoVE 1555

Lia Kent

Research and Development, Stemgent

Date Published: December 24, 2009  |  doi:10.3791/1555  |  Section: JoVE General

Keywords: Developmental Biology, Human embryonic stem cell, medium, hES, maintenance, thaw, ES cells, stem cell, cell culture, pluripotency, differentiation, passage, freeze

Engineering Cell-permeable Protein

JoVE 1627

Bernhard Münst, Christoph Patsch, Frank Edenhofer

Stem Cell Engineering Group, Institute of Reconstructive Neurobiology, University of Bonn - Life & Brain Center and Hertie Foundation

Date Published: December 28, 2009  |  doi:10.3791/1627  |  Section: JoVE General

Keywords: Cellular Biology, Protein transduction, Cell penetrating peptide, Site-specific recombination, Stem cells, Protein purification

Live Dissection of Drosophila Embryos: Streamlined Methods for Screening Mutant Collections by Antibody Staining

JoVE 1647

Hyung-Kook (Peter) Lee, Ashley P. Wright, Kai Zinn

Division of Biology, California Institute of Technology

Date Published: December 29, 2009  |  doi:10.3791/1647  |  Section: JoVE General

Keywords: Developmental Biology, Drosophila, dissection, live staining, neural development, immunohistochemistry, immunofluorescence, embryo, GFP, genetics, axon guidance, live dissection

A Practical Approach to Genetic Inducible Fate Mapping: A Visual Guide to Mark and Track Cells In Vivo

JoVE 1687

Ashly Brown¹, Stephen Brown², Debra Ellisor², Nellwyn Hagan¹, Elizabeth Normand¹, Mark Zervas²

¹Department of Neuroscience, Division of Biology and Medicine, Brown University, ²Department of Molecular Biology, Cell Biology and Biochemistry, Division of Biology and Medicine, Brown University

Date Published: December 30, 2009  |  doi:10.3791/1687  |  Section: JoVE General

Keywords: Developmental Biology, neurodevelopment, genetics, genetic inducible fate mapping (GIFM), immunostaining, mouse, embryo, GIFM, lineage tracer, fate mapping

Automated Microfluidic Blood Lysis Protocol for Enrichment of Circulating Nucleated Cells

JoVE 1656

William N. White¹, Palaniappan Sethu²

¹Department of Mechanical Engineering, University of Louisville, ²Department of Bioengineering, University of Louisville

Date Published: December 31, 2009  |  doi:10.3791/1656  |  Section: JoVE General

Keywords: Cellular Biology, Microfluidics, Blood lysis, Cell Enrichment, Circulating Nucleated Cells, leukocytes, flow cytometry, FACS