Table of Contents: Issue 28, June 2009

An interview with Nobel laureate Roy Glauber, Physics 2005

JoVE 1535

Roy Glauber

Date Published: June 26, 2009  |  doi:10.3791/1535  |  Section: JoVE Editorial

Keywords: Physics, Quanum Optics, Light, Wave Particle Duality, Los Alamos, Lindau, Nobel Laureate

Lens Transplantation in Zebrafish and its Application in the Analysis of Eye Mutants

JoVE 1258

Yan Zhang^1,2, Kyle McCulloch², Jarema Malicki²

¹The Second Teaching Hospital of Jilin University, ²Department of Ophthalmology, Harvard Medical School

Date Published: June 1, 2009  |  doi:10.3791/1258  |  Section: JoVE General

Keywords: Developmental Biology, Zebrafish, lens mutation, lens transplantation

Born Normalization for Fluorescence Optical Projection Tomography for Whole Heart Imaging

JoVE 1389

Claudio Vinegoni^1,2, Daniel Razansky³, Jose-Luiz Figueiredo^1,2, Lyuba Fexon^1,2, Misha Pivovarov^1,2, Matthias Nahrendorf^1,2, Vasilis Ntziachristos³, Ralph Weissleder^1,2

¹Center for Systems Biology, Harvard Medical School, ²Center for Systems Biology, MGH - Massachusetts General Hospital, ³Institute for Biological and Medical Imaging, Technical University of Munich and Helmholtz Center Munich

Date Published: June 2, 2009  |  doi:10.3791/1389  |  Section: JoVE General

Keywords: Bioengineering, optical imaging, fluorescence imaging, optical projection tomography, born normalization, molecular imaging, heart imaging

A Tactile Automated Passive-Finger Stimulator (TAPS)

JoVE 1374

Daniel Goldreich^1,2, Michael Wong², Ryan M. Peters², Ingrid M. Kanics¹

¹Department of Occupational Therapy, Duquesne University, ²Department of Psychology, Neuroscience and Behaviour, McMaster University

Date Published: June 3, 2009  |  doi:10.3791/1374  |  Section: JoVE General

Keywords: Medicine, Neuroscience, tactile, somatosensory, touch, cutaneous, acuity, psychophysics, Bayesian, grating orientation, sensory neuroscience, spatial discrimination

The Microfluidic Probe: Operation and Use for Localized Surface Processing

JoVE 1418

Cecile M. Perrault, Mohammad A. Qasaimeh, David Juncker

Department of Biomedical Engineering, McGill University

Date Published: June 4, 2009  |  doi:10.3791/1418  |  Section: JoVE General

Keywords: Bioengineering, microfluidics, integrated microfluidic system, bioMEMs

Dendra2 Photoswitching through the Mammary Imaging Window

JoVE 1278

Bojana Gligorijevic^1,2, Dmitriy Kedrin¹, Jeffrey E Segall¹, John Condeelis^1,2, Jacco van Rheenen^1,2,3

¹Department of Anatomy and Structural Biology, Albert Einstein College of Medicine - Yeshiva University, ²Gruss Lipper Biophotonics Center, Albert Einstein College of Medicine - Yeshiva University, ³Hubrecht Institute-KNAW and University Medical Center Utrecht

Date Published: June 5, 2009  |  doi:10.3791/1278  |  Section: JoVE General

Keywords: Cell Biology, microscopy, intravital, photoswitching, fluorescent proteins, imaging window, metastasis, intravasation, invasion, microenvironment

Preparation of Aplysia Sensory-motor Neuronal Cell Cultures

JoVE 1355

Yali Zhao¹, Dan O. Wang¹, Kelsey C. Martin^1,2,3

¹Dept. of Psychiatry and Biobehavioral Sciences, University of California, Los Angeles, ²Dept. of Biological Chemistry, University of California, Los Angeles, ³Semel Institute for Neuroscience and Human Behavior, University of California, Los Angeles

Date Published: June 8, 2009  |  doi:10.3791/1355  |  Section: JoVE General

Keywords: Neuroscience, Aplysia Californica, Synaptic Plasticity, Sensory Motor Neuronal Cultures, Invertebrates, Short-Term Facilitation, Monosynaptic, Intermediate-Term Facilitation, Ganglia, Long-Term Depression, Autapses, Sirnas, Glutamatergic Synapses, Somata

Imaging Exocytosis in Retinal Bipolar Cells with TIRF Microscopy

JoVE 1305

Christina Joselevitch, David Zenisek

Cellular and Molecular Physiology, Yale University School of Medicine

Date Published: June 9, 2009  |  doi:10.3791/1305  |  Section: JoVE General

Keywords: Neuroscience, Vision, retina, exocytosis, synaptic transmission, imaging,

Virus-induced Gene Silencing (VIGS) in Nicotiana benthamiana and Tomato

JoVE 1292

Andrá C. Velásquez^1,2, Suma Chakravarthy², Gregory B. Martin^1,2

¹Plant Pathology and Plant-Microbe Biology, Cornell University, ²Boyce Thompson Institute for Plant Research

Date Published: June 10, 2009  |  doi:10.3791/1292  |  Section: JoVE General

Keywords: Plant Biology, Virus-induced gene silencing (VIGS), RNA interference (RNAi), Tobacco Rattle Virus (TRV) vectors, Nicotiana benthamiana, tomato

Catheterization of Intestinal Loops in Ruminants

JoVE 1301

Richard R. E. Uwiera¹, John P. Kastelic², G. Douglas Inglis²

¹Department of Agricultural, Food and Nutritional Science, University of Alberta, ²Agriculture and Agri-Food Canada, Lethbridge Research Centre, Lethbridge

Date Published: June 11, 2009  |  doi:10.3791/1301  |  Section: JoVE General

Keywords: Medicine, Intestinal loop, catheters, ileum, ruminants, sheep

The Ladder Rung Walking Task: A Scoring System and its Practical Application.

JoVE 1204

Gerlinde A. Metz, Ian Q. Whishaw

Canadian Centre for Behavioural Neuroscience, Department of Neuroscience, University of Lethbridge

Date Published: June 12, 2009  |  doi:10.3791/1204  |  Section: JoVE General

Keywords: Neuroscience, rat, animal model of walking, skilled movement, ladder test, rung test, neuroscience

Monitoring Plant Hormones During Stress Responses

JoVE 1127

Marie J. Engelberth, Jurgen Engelberth

Department of Biology, University of Texas

Date Published: June 15, 2009  |  doi:10.3791/1127  |  Section: JoVE General

Keywords: Plant Biology, Jasmonic acid, salicylic acid, abscisic acid, plant hormones, GC/MS, vapor phase extraction

Primary Culture of Adult Rat Heart Myocytes

JoVE 1308

Xianghua Xu¹, Henry M. Colecraft^1,2

¹Department of Physiology and Cellular Biophysics, Columbia University, ²Department of Pharmacology, Columbia University

Date Published: June 16, 2009  |  doi:10.3791/1308  |  Section: JoVE General

Keywords: Cellular Biology, Rat, heart, isolation, primary culture, cardiology

Seawater Sampling and Collection

JoVE 1159

Elena Zaikova, Alyse Hawley, David A. Walsh, Steven J. Hallam

Department of Microbiology and Immunology, University of British Columbia - UBC

Date Published: June 17, 2009  |  doi:10.3791/1159  |  Section: JoVE General

Keywords: Molecular Biology, microbial biomass, nucleic acids, nutrients, trace gas, ammonia, sulfide, seawater, fjord, hypoxic, Saanich Inlet

Large Volume (20L+) Filtration of Coastal Seawater Samples

JoVE 1161

David A. Walsh, Elena Zaikova, Steven J. Hallam

Department of Microbiology and Immunology, University of British Columbia - UBC

Date Published: June 18, 2009  |  doi:10.3791/1161  |  Section: JoVE General

Keywords: Molecular Biology, microbial biomass, filtration, sterivex, GF/D, nucleic acids, seawater, fjord, hypoxic, Saanich Inlet

Small Volume (1-3L) Filtration of Coastal Seawater Samples

JoVE 1163

David A. Walsh, Elena Zaikova, Steven J. Hallam

Department of Microbiology and Immunology, University of British Columbia - UBC

Date Published: June 19, 2009  |  doi:10.3791/1163  |  Section: JoVE General

Keywords: Molecular Biology, microbiology, seawater, filtration, biomass concentration

Demonstration of Cutaneous Allodynia in Association with Chronic Pelvic Pain

JoVE 1232

John Jarrell

Department of Obstetrics and Gynecology, Faculty of Medicine, University of Calgary

Date Published: June 23, 2009  |  doi:10.3791/1232  |  Section: JoVE General

Keywords: Medicine, Chronic pelvic pain, cutaneous allodynia, trigger points, dysmenorrhea, endometriosis, dyspareunia

Implantation of Engineered Tissue in the Rat Heart

JoVE 1139

Bjoern Sill¹, Ivan V. Alpatov², Christina A. Pacak², Douglas B. Cowan²

¹Department of Anesthesiology, Perioperative and Pain Medicine, Children's Hospital Boston and Harvard Medical School, ²Department of Anesthesiology, Perioperative and Pain Medicine, Children’s Hospital Boston

Date Published: June 24, 2009  |  doi:10.3791/1139  |  Section: JoVE General

Keywords: Cellular Biology, thoracotomy, rodent surgery, anesthesia, atrioventricular, cardiac, tissue engineering, intubation

Measuring Replicative Life Span in the Budding Yeast

JoVE 1209

Kristan K. Steffen¹, Brian K. Kennedy¹, Matt Kaeberlein²

¹Department of Biochemistry, University of Washington, ²Department of Pathology, University of Washington

Date Published: June 25, 2009  |  doi:10.3791/1209  |  Section: JoVE General

Keywords: Developmental Biology, aging, longevity, life span, yeast, dietary restriction, Saccharomyces cerevisiae

Focal Ca²⁺ Transient Detection in Smooth Muscle

JoVE 1247

John S. Young, Robert J. Amos, Keith L. Brain

Department of Pharmacology, University of Oxford

Date Published: June 29, 2009  |  doi:10.3791/1247  |  Section: JoVE General

Keywords: Cellular Biology, Calcium, fluorescence, confocal, smooth muscle, autonomic, ATP

Making Gynogenetic Diploid Zebrafish by Early Pressure

JoVE 1396

Charline Walker¹, Greg S. Walsh², Cecilia Moens²

¹Institute of Neuroscience, University of Oregon, ²Division of Basic Science, Fred Hutchinson Cancer Research Center - FHCRC

Date Published: June 30, 2009  |  doi:10.3791/1396  |  Section: JoVE General

Keywords: Developmental Biology, Zebrafish, Early Pressure, Homozygous Diploid, Haploid, Gynogenesis