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General Laboratory Techniques

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Basic Methods in Cellular and Molecular Biology

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Model Organisms I

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Table of Contents

Table of Contents: Issue 54, August 2011

Jump to:  JoVE Editorial (1) JoVE Biology (19) JoVE Neuroscience (12) JoVE Immunology and Infection (7) JoVE Clinical and Translational Medicine (9) JoVE Bioengineering (8)
 JoVE Biology

Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay

1Department of Pediatrics and Department of Cancer Biology, University of Massachusetts Medical School


JoVE 2809

Date Published: August 5, 2011  |  doi:10.3791/2809  |  Section: JoVE Biology

 JoVE Biology

FSL Constructs: A Simple Method for Modifying Cell/Virion Surfaces with a Range of Biological Markers Without Affecting their Viability

1Biotechnology Research Institute, AUT University and KODE Biotech Ltd, 2Shemyakin Institute of Bioorganic Chemistry RAS, Moscow, Russia


JoVE 3289

Date Published: August 5, 2011  |  doi:10.3791/3289  |  Section: JoVE Biology

 JoVE Biology

Diagnostic Necropsy and Selected Tissue and Sample Collection in Rats and Mice

1Research Animal Diagnostic Services, Charles River, 2Research Models and Services, Charles River, 3Department of Comparative Medicine, University of Washington


JoVE 2966

Date Published: August 7, 2011  |  doi:10.3791/2966  |  Section: JoVE Biology

 JoVE Biology

Competitive Genomic Screens of Barcoded Yeast Libraries

1Banting and Best Department of Medical Research and Department of Molecular Genetics, University of Toronto, 2Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 3Donnelly Sequencing Centre, University of Toronto, 4Genetics and Molecular Biology Branch, National Human Genome Research Institute, NIH, 5Stanford Genome Technology Center, Stanford School of Medicine, Stanford University, 6Department of Pharmaceutical Sciences, University of Toronto


JoVE 2864

Date Published: August 11, 2011  |  doi:10.3791/2864  |  Section: JoVE Biology

 JoVE Biology

Protein Membrane Overlay Assay: A Protocol to Test Interaction Between Soluble and Insoluble Proteins in vitro

1Department of Biochemistry and Cell Biology, State University of New York


JoVE 2961

Date Published: August 14, 2011  |  doi:10.3791/2961  |  Section: JoVE Biology

 JoVE Biology

A High Throughput in situ Hybridization Method to Characterize mRNA Expression Patterns in the Fetal Mouse Lower Urogenital Tract

1Department of Comparative Biosciences, School of Veterinary Medicine, University of Wisconsin-Madison


JoVE 2912

Date Published: August 19, 2011  |  doi:10.3791/2912  |  Section: JoVE Biology

 JoVE Biology

Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton

1Department of Biochemistry and Biophysics, Institute of Plant Genomics and Biotechnology, Texas A&M University, 2Department of Plant Pathology and Microbiology, Institute of Plant Genomics and Biotechnology, Texas A&M University


JoVE 2938

Date Published: August 20, 2011  |  doi:10.3791/2938  |  Section: JoVE Biology

 JoVE Biology

Time-lapse Microscopy of Early Embryogenesis in Caenorhabditis elegans

1Department of Biological Sciences, University of Alabama in Huntsville, 2NIDDK-National Institutes of Health


JoVE 2852

Date Published: August 25, 2011  |  doi:10.3791/2852  |  Section: JoVE Biology

 JoVE Biology

ReAsH/FlAsH Labeling and Image Analysis of Tetracysteine Sensor Proteins in Cells

1Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute


JoVE 2857

Date Published: August 31, 2011  |  doi:10.3791/2857  |  Section: JoVE Biology

 JoVE Neuroscience

Mosaic Analysis of Gene Function in Postnatal Mouse Brain Development by Using Virus-based Cre Recombination

1Neuroscience Graduate Program, Keck School of Medicine, University of Southern California, 2Zilkha Neurogenetic Institute, University of Southern California, 3Department of Cell and Neurobiology, Neuroscience Graduate Program, Keck School of Medicine, University of Southern California


JoVE 2823

Date Published: August 1, 2011  |  doi:10.3791/2823  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Multi-electrode Array Recordings of Neuronal Avalanches in Organotypic Cultures

1Section on Critical Brain Dynamics, National Institute of Mental Health


JoVE 2949

Date Published: August 1, 2011  |  doi:10.3791/2949  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Targeted Training of Ultrasonic Vocalizations in Aged and Parkinsonian Rats

1Department of Surgery-Division of Otolaryngology, University of Wisconsin, 2Department of Communicative Disorders, University of Wisconsin


JoVE 2835

Date Published: August 8, 2011  |  doi:10.3791/2835  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Targeting Olfactory Bulb Neurons Using Combined In Vivo Electroporation and Gal4-Based Enhancer Trap Zebrafish Lines

1Department of Biology, Pace University, 2Cellular and Molecular Medicine, University of California, San Diego, 3Division of Cell Biology and Cell Physiology, Zoological Institute, Braunschweig University of Technology


JoVE 2964

Date Published: August 15, 2011  |  doi:10.3791/2964  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection

1Lab for Molecular Mechanisms of Thalamus Development, RIKEN Brain Science Institute


JoVE 3024

Date Published: August 15, 2011  |  doi:10.3791/3024  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Derivation of Enriched Oligodendrocyte Cultures and Oligodendrocyte/Neuron Myelinating Co-cultures from Post-natal Murine Tissues

1Regenerative Medicine Program, Ottawa Hospital Research Institute, 2Department of Cellular and Molecular Medicine, University of Ottawa, 3Department of Pharmacological Sciences, Stony Brook University, 4Department of Medicine, University of Ottawa


JoVE 3324

Date Published: August 21, 2011  |  doi:10.3791/3324  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Isolating Nasal Olfactory Stem Cells from Rodents or Humans

1NICN, Aix Marseille University, 2LNPM, Aix Marseille University, 3ENT Department, Aix Marseille University, 4Gene expression Laboratory, The Salk Institute for Biological Studies, 5Laboratory of Speech and Language, Aix Marseille University, 6Centre d'Investigations Cliniques en Biothérapie, Aix Marseille University


JoVE 2762

Date Published: August 22, 2011  |  doi:10.3791/2762  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Lateral Fluid Percussion: Model of Traumatic Brain Injury in Mice

1Department of Neuroscience and Cell Biology, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, 2Spinal Cord and Brain Injury Research Center, 3Department of Anatomy and Neurobiology, Department of Physical Medicine and Rehabilitation, University of Kentucky Chandler Medical Center


JoVE 3063

Date Published: August 22, 2011  |  doi:10.3791/3063  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Autologous Blood Injection to Model Spontaneous Intracerebral Hemorrhage in Mice

1Department of Neurology, University of Connecticut Health Center, 2Department of Neurology, School of Medicine, University of Pennsylvania, 3Department of Neurosurgery, Hartford Hospital, 4Department of Neurosurgery, School of Medicine, University of Pennsylvania


JoVE 2618

Date Published: August 24, 2011  |  doi:10.3791/2618  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Brain Imaging Investigation of the Neural Correlates of Emotional Autobiographical Recollection

1Department of Psychiatry, University of Alberta, Edmonton, 2Psychology Department, University of Illinois, Urbana-Champaign, 3Neuroscience Program, University of Illinois, Urbana-Champaign, 4Beckman Institute for Advanced Science & Technology, University of Illinois, Urbana-Champaign


JoVE 2396

Date Published: August 26, 2011  |  doi:10.3791/2396  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Brain Imaging Investigation of the Neural Correlates of Emotion Regulation

1Department of Psychology, University of Illinois, Urbana-Champaign, 2Department of Computing Science, University of Alberta, Edmonton, 3Department of Psychiatry, University of Alberta, Edmonton, 4Department of Psychology, University of Alberta, Edmonton, 5Centre for Neuroscience, University of Alberta, Edmonton, 6Neuroscience Program, University of Illinois, Urbana-Champaign, 7Beckman Institute, University of Illinois, Urbana-Champaign


JoVE 2430

Date Published: August 26, 2011  |  doi:10.3791/2430  |  Section: JoVE Neuroscience

 JoVE Immunology and Infection

Rapid Diagnosis of Avian Influenza Virus in Wild Birds: Use of a Portable rRT-PCR and Freeze-dried Reagents in the Field

1USGS Western Ecological Research Center, 2Wildlife Health Center, University of California, Davis, 3Department of Population Health and Reproduction, University of California, Davis, 4Department of Veterinary and Biomedical Sciences, University of Minnesota, 5Science Applications International Corporation


JoVE 2829

Date Published: August 2, 2011  |  doi:10.3791/2829  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Avian Influenza Surveillance with FTA Cards: Field Methods, Biosafety, and Transportation Issues Solved

1Resource Ecology Group, Wageningen University, 2Section for Zoonotic Ecology and Epidemiology, School of Natural Sciences, Linnaeus University, 3Centre for Wildlife Ecology, Simon Fraser University


JoVE 2832

Date Published: August 2, 2011  |  doi:10.3791/2832  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction

1Department of Structural Biology, University of Pittsburgh School of Medicine


JoVE 3041

Date Published: August 9, 2011  |  doi:10.3791/3041  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes

1St Vincent’s Institute, Department of Medicine, The University of Melbourne, 2Department of Microbiology and Immunology, The University of Melbourne


JoVE 3076

Date Published: August 9, 2011  |  doi:10.3791/3076  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Cercarial Transformation and in vitro Cultivation of Schistosoma mansoni Schistosomules

1Department of Biology, Case Western Reserve University


JoVE 3191

Date Published: August 16, 2011  |  doi:10.3791/3191  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Imaging Leukocyte Adhesion to the Vascular Endothelium at High Intraluminal Pressure

1Vascular Pharmacology Laboratory, Baker IDI Heart and Diabetes Institute, Monash University


JoVE 3221

Date Published: August 23, 2011  |  doi:10.3791/3221  |  Section: JoVE Immunology and Infection

 JoVE Clinical and Translational Medicine

In vivo Near Infrared Fluorescence (NIRF) Intravascular Molecular Imaging of Inflammatory Plaque, a Multimodal Approach to Imaging of Atherosclerosis

1Cardiovascular Research Center and Cardiology Division, Massachusetts General Hospital, Harvard Medical School, 2Institute for Biological and Medical Imaging, Helmholtz Zentrum München und Technische Universität München, 3Department of Electrical and Computer Engineering, Northeastern University


JoVE 2257

Date Published: August 4, 2011  |  doi:10.3791/2257  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Establishment and Propagation of Human Retinoblastoma Tumors in Immune Deficient Mice

1Interdepartmental Program in Translational Biology & Molecular Medicine, Baylor College of Medicine, 2Texas Children's Cancer Center, Baylor College of Medicine, 3Department of Pediatrics, Baylor College of Medicine, 4Department of Pathology, The Methodist Hospital Research Institute, 5Department of Ophthalmology, Retinoblastoma Center of Houston, 6Baylor College of Medicine, Center for Cell and Gene Therapy, 7Center for Cell and Gene Therapy, Baylor College of Medicine


JoVE 2644

Date Published: August 4, 2011  |  doi:10.3791/2644  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

An In Vitro System to Study Tumor Dormancy and the Switch to Metastatic Growth

1Department of Biology, University of Haifa, 2Transgenic Oncogenesis and Genomics Section, Laboratory of Cancer Biology and Genetics, National Cancer Institute


JoVE 2914

Date Published: August 11, 2011  |  doi:10.3791/2914  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

The Spared Nerve Injury (SNI) Model of Induced Mechanical Allodynia in Mice

1The Lundbeck Foundation Research Center MIND, Department of Biomedicine, Aarhus University, 2Department of Pharmacology and Pharmacotherapy, Faculty of Pharmaceutical Sciences, University of Copenhagen


JoVE 3092

Date Published: August 18, 2011  |  doi:10.3791/3092  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Biochemical Measurement of Neonatal Hypoxia

1Division of Biochemistry, Department of Basic Sciences, Loma Linda University, 2Division of Physiology, Department of Basic Sciences, Loma Linda University


JoVE 2948

Date Published: August 24, 2011  |  doi:10.3791/2948  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Normothermic Cardiac Arrest and Cardiopulmonary Resuscitation: A Mouse Model of Ischemia-Reperfusion Injury

1Department of Anesthesiology and Perioperative Medicine, Oregon Health & Sciences University, 2Department of Pharmacology, University of Colorado Denver


JoVE 3116

Date Published: August 30, 2011  |  doi:10.3791/3116  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Examining the Characteristics of Episodic Memory using Event-related Potentials in Patients with Alzheimer's Disease

1Department of Neurology, Vanderbilt University


JoVE 2715

Date Published: August 30, 2011  |  doi:10.3791/2715  |  Section: JoVE Clinical and Translational Medicine

 JoVE Bioengineering

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

1The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco, 2Center for Reproductive Sciences, University of California San Francisco, 3Department of Urology, University of California San Francisco, 4Department of Cell and Tissue Biology, University of California San Francisco, 5Fluidigm Corporation, Fluidigm Corporation, 6Department of Obstetrics and Gynecology, Hadassah-Hebrew University Medical Center, 7UCSF - Helen Diller Family Comprehensive Cancer Center, University of California San Francisco


JoVE 2552

Date Published: August 3, 2011  |  doi:10.3791/2552  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Haptic/Graphic Rehabilitation: Integrating a Robot into a Virtual Environment Library and Applying it to Stroke Therapy

1Department of Bioengineering, University of Illinois at Chicago and Rehabilitation Institute of Chicago, 2Sensory Motor Performance Program, Rehabilitation Institute of Chicago


JoVE 3007

Date Published: August 8, 2011  |  doi:10.3791/3007  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Axon Stretch Growth: The Mechanotransduction of Neuronal Growth

1Departments of Biomedical Engineering, New Jersey Institute of Technology, 2Graduate School of Biomedical Sciences, University of Medicine and Dentistry of New Jersey


JoVE 2753

Date Published: August 10, 2011  |  doi:10.3791/2753  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Bacterial Immobilization for Imaging by Atomic Force Microscopy

1Biological and Nanoscale Systems Group, Biosciences Division, Oak Ridge National Laboratory, 2Department of Biochemistry and Cellular and Molecular Biology, University of Tennessee, 3Department of Surgery, Eastern Virginia Medical School, 4Center for Nanophase Materials Sciences Division, Oak Ridge National Laboratory


JoVE 2880

Date Published: August 10, 2011  |  doi:10.3791/2880  |  Section: JoVE Bioengineering

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