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The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.

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Table of Contents

Table of Contents: Issue 54, August 2011

Jump to:  JoVE Editorial (1) JoVE General (19) JoVE Neuroscience (12) JoVE Immunology and Infection (7) JoVE Clinical and Translational Medicine (9) JoVE Bioengineering (8)
 JoVE General

Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay


JoVE 2809

*, *, *, *, ,

Department of Pediatrics and Department of Cancer Biology, University of Massachusetts Medical School

Date Published: August 5, 2011  |  doi:10.3791/2809  |  Section: JoVE General

Keywords: Developmental Biology, erythropoiesis, hematopoietic progenitors, flow-cytometry, erythropoietin, EpoR-/- mouse, erythropoietic stress, fetal erythropoiesis, CD71, Ter119, Fetal liver, erythroid subsets, erythroblast, cell cycle

 JoVE General

FSL Constructs: A Simple Method for Modifying Cell/Virion Surfaces with a Range of Biological Markers Without Affecting their Viability


JoVE 3289

1, 2, 1, 1

1Biotechnology Research Institute, AUT University and KODE Biotech Ltd, 2Shemyakin Institute of Bioorganic Chemistry RAS, Moscow, Russia

Date Published: August 5, 2011  |  doi:10.3791/3289  |  Section: JoVE General

Keywords: Molecular Biology, kodecyte, FSL construct, imaging, biotin, fluorophore

 JoVE General

Diagnostic Necropsy and Selected Tissue and Sample Collection in Rats and Mice


JoVE 2966

1, 1, 1, 1, 2, 2,3

1Research Animal Diagnostic Services, Charles River, 2Research Models and Services, Charles River, 3Department of Comparative Medicine, University of Washington

Date Published: August 7, 2011  |  doi:10.3791/2966  |  Section: JoVE General

Keywords: Anatomy, rodent, necropsy, diagnostic assay, bacteriology, PCR, organ collection, tissue sampling

 JoVE General

Competitive Genomic Screens of Barcoded Yeast Libraries


JoVE 2864

*1,2, *2,3, *4, 1,2, 5, 2,3, 2,6, 1,2,3

1Banting and Best Department of Medical Research and Department of Molecular Genetics, University of Toronto, 2Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 3Donnelly Sequencing Centre, University of Toronto, 4Genetics and Molecular Biology Branch, National Human Genome Research Institute, NIH, 5Stanford Genome Technology Center, Stanford School of Medicine, Stanford University, 6Department of Pharmaceutical Sciences, University of Toronto

Date Published: August 11, 2011  |  doi:10.3791/2864  |  Section: JoVE General

Keywords: Biochemistry, chemical biology, chemogenomics, chemical probes, barcode microarray, next generation sequencing

 JoVE General

A High Throughput in situ Hybridization Method to Characterize mRNA Expression Patterns in the Fetal Mouse Lower Urogenital Tract


JoVE 2912

, , , , , , ,

Department of Comparative Biosciences, School of Veterinary Medicine, University of Wisconsin-Madison

Date Published: August 19, 2011  |  doi:10.3791/2912  |  Section: JoVE General

Keywords: Developmental Biology, Urogenital, prostate, lower urinary tract, urethra, in situ hybridization

 JoVE General

Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton


JoVE 2938

1, 1, 2, 1

1Department of Biochemistry and Biophysics, Institute of Plant Genomics and Biotechnology, Texas A&M University, 2Department of Plant Pathology and Microbiology, Institute of Plant Genomics and Biotechnology, Texas A&M University

Date Published: August 20, 2011  |  doi:10.3791/2938  |  Section: JoVE General

Keywords: Plant Biology, Agrobacterium, Cotton, Functional Genomics, Virus-Induced Gene Silencing

 JoVE Neuroscience

Mosaic Analysis of Gene Function in Postnatal Mouse Brain Development by Using Virus-based Cre Recombination


JoVE 2823

1,2, 2,3

1Neuroscience Graduate Program, Keck School of Medicine, University of Southern California, 2Zilkha Neurogenetic Institute, University of Southern California, 3Department of Cell and Neurobiology, Neuroscience Graduate Program, Keck School of Medicine, University of Southern California

Date Published: August 1, 2011  |  doi:10.3791/2823  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Adeno-associated virus, Cre, mosaic analysis, sparse labeling, mouse, postnatal, brain development

 JoVE Neuroscience

Multi-electrode Array Recordings of Neuronal Avalanches in Organotypic Cultures


JoVE 2949

, , , , ,

Section on Critical Brain Dynamics, National Institute of Mental Health

Date Published: August 1, 2011  |  doi:10.3791/2949  |  Section: JoVE Neuroscience

Keywords: Neuroscience, neuronal activity, neuronal avalanches, organotypic culture, slice culture, microelectrode array, electrophysiology, local field potential, extracellular spikes

 JoVE Neuroscience

Targeted Training of Ultrasonic Vocalizations in Aged and Parkinsonian Rats


JoVE 2835

1,2, 2, 2, 2, 2, 1,2

1Department of Surgery-Division of Otolaryngology, University of Wisconsin, 2Department of Communicative Disorders, University of Wisconsin

Date Published: August 8, 2011  |  doi:10.3791/2835  |  Section: JoVE Neuroscience

Keywords: Neuroscience, ultrasonic vocalization, rat, aging, Parkinson disease, exercise, 6-hydroxydopamine, voice disorders, voice therapy

 JoVE Neuroscience

Targeting Olfactory Bulb Neurons Using Combined In Vivo Electroporation and Gal4-Based Enhancer Trap Zebrafish Lines


JoVE 2964

1, 2, 3, 1

1Department of Biology, Pace University, 2Cellular and Molecular Medicine, University of California, San Diego, 3Division of Cell Biology and Cell Physiology, Zoological Institute, Braunschweig University of Technology

Date Published: August 15, 2011  |  doi:10.3791/2964  |  Section: JoVE Neuroscience

Keywords: Neuroscience, electroporation, zebrafish, olfactory bulb, Gal4 enhancer trap

 JoVE Neuroscience

Derivation of Enriched Oligodendrocyte Cultures and Oligodendrocyte/Neuron Myelinating Co-cultures from Post-natal Murine Tissues


JoVE 3324

1,2, 1, 3, 1,2,4

1Regenerative Medicine Program, Ottawa Hospital Research Institute, 2Department of Cellular and Molecular Medicine, University of Ottawa, 3Department of Pharmacological Sciences, Stony Brook University, 4Department of Medicine, University of Ottawa

Date Published: August 21, 2011  |  doi:10.3791/3324  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Oligodendrocyte, myelination, in vitro, dorsal root ganglion neuron, co-culture, primary cells, mouse, neuroscience

 JoVE Neuroscience

Isolating Nasal Olfactory Stem Cells from Rodents or Humans


JoVE 2762

1,2, 3, 1,2,4, 5, 2, 1,6

1NICN, Aix Marseille University, 2LNPM, Aix Marseille University, 3ENT Department, Aix Marseille University, 4Gene expression Laboratory, The Salk Institute for Biological Studies, 5Laboratory of Speech and Language, Aix Marseille University, 6Centre d'Investigations Cliniques en Biothérapie, Aix Marseille University

Date Published: August 22, 2011  |  doi:10.3791/2762  |  Section: JoVE Neuroscience

Keywords: Neuroscience, stem cell, nose, brain, neuron, cell therapy, diagnosis, sphere

 JoVE Neuroscience

Lateral Fluid Percussion: Model of Traumatic Brain Injury in Mice


JoVE 3063

1, 1, 2,3, 1, 1

1Department of Neuroscience and Cell Biology, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, 2Spinal Cord and Brain Injury Research Center, 3Department of Anatomy and Neurobiology, Department of Physical Medicine and Rehabilitation, University of Kentucky Chandler Medical Center

Date Published: August 22, 2011  |  doi:10.3791/3063  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Lateral fluid percussion, hippocampus, traumatic brain injury, Morris Water Maze, mouse model of moderate injury

 JoVE Neuroscience

Autologous Blood Injection to Model Spontaneous Intracerebral Hemorrhage in Mice


JoVE 2618

1,2,3, 2, 1, 2, 4, 4

1Department of Neurology, University of Connecticut Health Center, 2Department of Neurology, School of Medicine, University of Pennsylvania, 3Department of Neurosurgery, Hartford Hospital, 4Department of Neurosurgery, School of Medicine, University of Pennsylvania

Date Published: August 24, 2011  |  doi:10.3791/2618  |  Section: JoVE Neuroscience

Keywords: Neuroscience, stroke, intracerebral hemorrhage, mice, animal model

 JoVE Neuroscience

Brain Imaging Investigation of the Neural Correlates of Emotional Autobiographical Recollection


JoVE 2396

1, 1, 1,2, 1,2,3,4

1Department of Psychiatry, University of Alberta, Edmonton, 2Psychology Department, University of Illinois, Urbana-Champaign, 3Neuroscience Program, University of Illinois, Urbana-Champaign, 4Beckman Institute for Advanced Science & Technology, University of Illinois, Urbana-Champaign

Date Published: August 26, 2011  |  doi:10.3791/2396  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Personal Memories, Retrieval Focus, Cognitive Distraction, Emotion Regulation, Neuroimaging

 JoVE Neuroscience

Brain Imaging Investigation of the Neural Correlates of Emotion Regulation


JoVE 2430

1, 2, 3, 4,5, 1,6,7

1Department of Psychology, University of Illinois, Urbana-Champaign, 2Department of Computing Science, University of Alberta, Edmonton, 3Department of Psychiatry, University of Alberta, Edmonton, 4Department of Psychology, University of Alberta, Edmonton, 5Centre for Neuroscience, University of Alberta, Edmonton, 6Neuroscience Program, University of Illinois, Urbana-Champaign, 7Beckman Institute, University of Illinois, Urbana-Champaign

Date Published: August 26, 2011  |  doi:10.3791/2430  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Emotion Suppression, Automatic Emotion Control, Deliberate Emotion Control, Goal Induction, Neuroimaging

 JoVE Immunology and Infection

Rapid Diagnosis of Avian Influenza Virus in Wild Birds: Use of a Portable rRT-PCR and Freeze-dried Reagents in the Field


JoVE 2829

1, 1,2, 1, 1, 3,4, 2, 5

1USGS Western Ecological Research Center, 2Wildlife Health Center, University of California, Davis, 3Department of Population Health and Reproduction, University of California, Davis, 4Department of Veterinary and Biomedical Sciences, University of Minnesota, 5Science Applications International Corporation

Date Published: August 2, 2011  |  doi:10.3791/2829  |  Section: JoVE Immunology and Infection

Keywords: Immunology, migratory birds, active surveillance, lyophilized reagents, avian influenza, H5N1

 JoVE Immunology and Infection

Avian Influenza Surveillance with FTA Cards: Field Methods, Biosafety, and Transportation Issues Solved


JoVE 2832

1, 1, 2, 2, 1,3, 1

1Resource Ecology Group, Wageningen University, 2Section for Zoonotic Ecology and Epidemiology, School of Natural Sciences, Linnaeus University, 3Centre for Wildlife Ecology, Simon Fraser University

Date Published: August 2, 2011  |  doi:10.3791/2832  |  Section: JoVE Immunology and Infection

Keywords: Immunology, AI, Influenza A Virus, zoonoses, reverse transcription PCR, viral RNA, surveillance, duck trap, RNA preservation and storage, infection, mallard

 JoVE Immunology and Infection

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction


JoVE 3041

, ,

Department of Structural Biology, University of Pittsburgh School of Medicine

Date Published: August 9, 2011  |  doi:10.3791/3041  |  Section: JoVE Immunology and Infection

Keywords: Immunology, cryo-electron microscopy, helical indexing, helical real-space reconstruction, tubular assemblies, HIV-1 capsid

 JoVE Immunology and Infection

Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes


JoVE 3076

1, 2, 2, 1

1St Vincent’s Institute, Department of Medicine, The University of Melbourne, 2Department of Microbiology and Immunology, The University of Melbourne

Date Published: August 9, 2011  |  doi:10.3791/3076  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Listeria, intracellular bacteria, genetic susceptibility, liver, spleen, blood, FACS analysis, T cells

 JoVE Immunology and Infection

Imaging Leukocyte Adhesion to the Vascular Endothelium at High Intraluminal Pressure


JoVE 3221

, , ,

Vascular Pharmacology Laboratory, Baker IDI Heart and Diabetes Institute, Monash University

Date Published: August 23, 2011  |  doi:10.3791/3221  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Leukocyte adhesion, intraluminal pressure, endothelial dysfunction, inflammation, hypertension

 JoVE Clinical and Translational Medicine

In vivo Near Infrared Fluorescence (NIRF) Intravascular Molecular Imaging of Inflammatory Plaque, a Multimodal Approach to Imaging of Atherosclerosis


JoVE 2257

1, 1,2, 1,3, 1, 2, 2, 1

1Cardiovascular Research Center and Cardiology Division, Massachusetts General Hospital, Harvard Medical School, 2Institute for Biological and Medical Imaging, Helmholtz Zentrum München und Technische Universität München, 3Department of Electrical and Computer Engineering, Northeastern University

Date Published: August 4, 2011  |  doi:10.3791/2257  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Atherosclerosis, inflammation, imaging, near infrared fluorescence, plaque, intravascular, catheter

 JoVE Clinical and Translational Medicine

Establishment and Propagation of Human Retinoblastoma Tumors in Immune Deficient Mice


JoVE 2644

1,2, 2,3, 2,3, 4, 2,3, *2,3,5,6, *4,5,7

1Interdepartmental Program in Translational Biology & Molecular Medicine, Baylor College of Medicine, 2Texas Children's Cancer Center, Baylor College of Medicine, 3Department of Pediatrics, Baylor College of Medicine, 4Department of Pathology, The Methodist Hospital Research Institute, 5Department of Ophthalmology, Retinoblastoma Center of Houston, 6Baylor College of Medicine, Center for Cell and Gene Therapy, 7Center for Cell and Gene Therapy, Baylor College of Medicine

Date Published: August 4, 2011  |  doi:10.3791/2644  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, retinoblastoma, tumor, xenograft, tumorsphere, mouse, human, eye, cancer stem cell

 JoVE Clinical and Translational Medicine

An In Vitro System to Study Tumor Dormancy and the Switch to Metastatic Growth


JoVE 2914

1, 2

1Department of Biology, University of Haifa, 2Transgenic Oncogenesis and Genomics Section, Laboratory of Cancer Biology and Genetics, National Cancer Institute

Date Published: August 11, 2011  |  doi:10.3791/2914  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Tumor dormancy, cancer recurrence, metastasis, reconstituted basement membrane extract (BME), 3D culture, breast cancer

 JoVE Clinical and Translational Medicine

The Spared Nerve Injury (SNI) Model of Induced Mechanical Allodynia in Mice


JoVE 3092

1, 2, 1, 1

1The Lundbeck Foundation Research Center MIND, Department of Biomedicine, Aarhus University, 2Department of Pharmacology and Pharmacotherapy, Faculty of Pharmaceutical Sciences, University of Copenhagen

Date Published: August 18, 2011  |  doi:10.3791/3092  |  Section: JoVE Clinical and Translational Medicine

Keywords: Neuroscience, Sciatic, Injury, PNS, Mechanical allodynia, Neuropathic pain, von Frey

 JoVE Clinical and Translational Medicine

Biochemical Measurement of Neonatal Hypoxia


JoVE 2948

1, 1, 2, 1, 2

1Division of Biochemistry, Department of Basic Sciences, Loma Linda University, 2Division of Physiology, Department of Basic Sciences, Loma Linda University

Date Published: August 24, 2011  |  doi:10.3791/2948  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, hypoxia, Ischemia, Neonate, Hypoxanthine, Xanthine, Uric Acid, Allantoin, Xanthine Oxidase, Malondialdehyde

 JoVE Clinical and Translational Medicine

Normothermic Cardiac Arrest and Cardiopulmonary Resuscitation: A Mouse Model of Ischemia-Reperfusion Injury


JoVE 3116

1, 2, 1, 1, 1

1Department of Anesthesiology and Perioperative Medicine, Oregon Health & Sciences University, 2Department of Pharmacology, University of Colorado Denver

Date Published: August 30, 2011  |  doi:10.3791/3116  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, AKI, Acute Kidney Injury, Acute Renal Failure, Cardiac Arrest, Cardiopulmonary Resuscitation, Mouse Model, Chest Compressions, CA/CPR. stereology, perfusion-fixation

 JoVE Clinical and Translational Medicine

Examining the Characteristics of Episodic Memory using Event-related Potentials in Patients with Alzheimer's Disease


JoVE 2715

,

Department of Neurology, Vanderbilt University

Date Published: August 30, 2011  |  doi:10.3791/2715  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, recognition memory, episodic memory, event-related potentials, dual process, Alzheimer's disease, amnestic mild cognitive impairment

 JoVE Bioengineering

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs


JoVE 2552

1,2,3, 1,2,4, 5, 1,2,6, 1,2,3,7

1The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco, 2Center for Reproductive Sciences, University of California San Francisco, 3Department of Urology, University of California San Francisco, 4Department of Cell and Tissue Biology, University of California San Francisco, 5Fluidigm Corporation, Fluidigm Corporation, 6Department of Obstetrics and Gynecology, Hadassah-Hebrew University Medical Center, 7UCSF - Helen Diller Family Comprehensive Cancer Center, University of California San Francisco

Date Published: August 3, 2011  |  doi:10.3791/2552  |  Section: JoVE Bioengineering

Keywords: Bioengineering, microRNA, multiplex qRT-PCR, high throughput profiling, Fluidigm

 JoVE Bioengineering

Haptic/Graphic Rehabilitation: Integrating a Robot into a Virtual Environment Library and Applying it to Stroke Therapy


JoVE 3007

1, 1, 2, 2

1Department of Bioengineering, University of Illinois at Chicago and Rehabilitation Institute of Chicago, 2Sensory Motor Performance Program, Rehabilitation Institute of Chicago

Date Published: August 8, 2011  |  doi:10.3791/3007  |  Section: JoVE Bioengineering

Keywords: Bioengineering, robotics, haptics, virtual reality, wrapper class, rehabilitation robotics, neural engineering, H3DAPI, C++

 JoVE Bioengineering

Axon Stretch Growth: The Mechanotransduction of Neuronal Growth


JoVE 2753

1,2, 1,2, 1

1Departments of Biomedical Engineering, New Jersey Institute of Technology, 2Graduate School of Biomedical Sciences, University of Medicine and Dentistry of New Jersey

Date Published: August 10, 2011  |  doi:10.3791/2753  |  Section: JoVE Bioengineering

Keywords: Bioengineering, tissue engineering, live imaging, axon stretch growth, nerve development, neuron, neuroscience

 JoVE Bioengineering

Bacterial Immobilization for Imaging by Atomic Force Microscopy


JoVE 2880

1,2, 3, 1,2, 1,4, 1,4

1Biological and Nanoscale Systems Group, Biosciences Division, Oak Ridge National Laboratory, 2Department of Biochemistry and Cellular and Molecular Biology, University of Tennessee, 3Department of Surgery, Eastern Virginia Medical School, 4Center for Nanophase Materials Sciences Division, Oak Ridge National Laboratory

Date Published: August 10, 2011  |  doi:10.3791/2880  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Bacteria, AFM imaging, Liquid imaging, Gelatin, Bacterial Immobilization

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