- 70: December 2012
- 69: November 2012
- 68: October 2012
- 67: September 2012
- 66: August 2012
- 65: July 2012
- 64: June 2012
- 63: May 2012
- 62: April 2012
- 61: March 2012
- 60: February 2012
- 59: January 2012
- 58: December 2011
- 57: November 2011
- 56: October 2011
- 55: September 2011
- 54: August 2011
- 53: July 2011
- 52: June 2011
- 51: May 2011
- 50: April 2011
- 49: March 2011
- 48: February 2011
- 47: January 2011
- 46: December 2010
- 45: November 2010
- 44: October 2010
- 43: September 2010
- 42: August 2010
- 41: July 2010
- 40: June 2010
- 39: May 2010
- 38: April 2010
- 37: March 2010
- 36: February 2010
- 35: January 2010
- 34: December 2009
- 33: November 2009
- 32: October 2009
- 31: September 2009
- 30: August 2009
- 29: July 2009
- 28: June 2009
- 27: May 2009
- 26: April 2009
- 25: March 2009
- 24: February 2009
- 23: January 2009
- 22: December 2008
- 21: November 2008
- 20: October 2008
- 19: September 2008
- 18: August 2008
- 17: July 2008
- 16: June 2008
- 15: May 2008
- 14: April 2008
- 13: March 2008
- 12: February 2008
- 11: January 2008
- 10: December 2007
- 9: November 2007
- 8: October 2007
- 7: August/September 2007
- 6: July/August 2007
- 5: June 2007
- 4: May 2007
- 3: April 2007
- 2: December-February 2006-07
Table of Contents
Table of Contents: Issue 32, October 2009
A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples
1Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco - UCSF, 2Buck Institute for Age Research, 3Department of Chemistry, Purdue University
Date Published: October 1, 2009 | doi:10.3791/1398 | Section: JoVE General
Keywords: Basic Protocols, Lectins, chromatography, glycopeptides, glycoproteins, biomarker discovery
Lentivirus Production
Diabetes Center, University of California, San Francisco - UCSF
Date Published: October 2, 2009 | doi:10.3791/1499 | Section: JoVE General
Keywords: Microbiology, Lentivirus, RNAi, viral titration, transfection, retrovirus, flow cytometry, split vector system, shRNA.
Anterior Cervical Discectomy and Fusion in the Ovine Model
1Monash Immunology and Stem Cell Laboratories (MISCL), Monash University, 2Department of Surgery, Monash University
Date Published: October 5, 2009 | doi:10.3791/1548 | Section: JoVE General
Keywords: Medicine, Anterior cervical discectomy, interbody fusion, spine fusion, stem cells, biologics, spine instrumentation, interbody cage
Mouse Mammary Epithelial Cells form Mammospheres During Lactogenic Differentiation
Date Published: October 6, 2009 | doi:10.3791/1265 | Section: JoVE General
Keywords: Cellular Biology, Mammospheres, HC11, lactogenic differentiation, mammary
Antibody Profiling by Luciferase Immunoprecipitation Systems (LIPS)
Date Published: October 7, 2009 | doi:10.3791/1549 | Section: JoVE General
Keywords: Immunology, Antigen, Autoantibodies, Biomarker, Diagnostic, ELISA, Proteome
Isolation and Animal Serum Free Expansion of Human Umbilical Cord Derived Mesenchymal Stromal Cells (MSCs) and Endothelial Colony Forming Progenitor Cells (ECFCs)
Stem Cell Research Unit, Medical University of Graz, Austria
Date Published: October 8, 2009 | doi:10.3791/1525 | Section: JoVE General
Keywords: Cellular Biology, Human adult progenitor cells, mesenchymal stromal cells (MSCs), endothelial colony forming progenitor cells (ECFCs), umbilical cord
Visualizing Single-molecule DNA Replication with Fluorescence Microscopy
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School
Date Published: October 9, 2009 | doi:10.3791/1529 | Section: JoVE General
Keywords: Cellular Biology, single-molecule, fluorescence, DNA replication, biophysics
Quantitative Phosphoproteomics in Fatty Acid Stimulated Saccharomyces cerevisiae
Date Published: October 12, 2009 | doi:10.3791/1474 | Section: JoVE General
Keywords: Cellular Biology, Phosphorylation, Proteomics, Cryolysis, Yeast, HILIC, IMAC, Oleate, SILAC
Fluorescent Labeling of Drosophila Heart Structures
1Biology Department, San Diego State University, 2Development and Aging Program, NASCR Center, The Sanford Burnham Institute for Medical Research
Date Published: October 13, 2009 | doi:10.3791/1423 | Section: JoVE General
Keywords: Cellular Biology, Cardiac, cardiomyopathy, dorsal vessel, fluorescence, staining, GFP, larva, immunohistochemistry, microscopy, imaging
LAD-Ligation: A Murine Model of Myocardial Infarction
1Transplant and Stem Cell Immunobiology Lab (TSI), University Heart Center Hamburg, 2CVRC, University Hospital Hamburg, 3Department of CT Surgery, Stanford University School of Medicine
Date Published: October 14, 2009 | doi:10.3791/1438 | Section: JoVE General
Keywords: Medicine, myocardial infarction, mice, LAD ligation, ischemia, histology, validation
Measures of Heart and Ventilatory Rates in Freely Moving Crayfish
Department of Biology, University of Kentucky
Date Published: October 15, 2009 | doi:10.3791/1594 | Section: JoVE General
Keywords: Physiology, invertebrate, autonomic nervous system, behavior, crustacean
Generation of Human CD40-activated B cells
Date Published: October 16, 2009 | doi:10.3791/1373 | Section: JoVE General
Keywords: Immunology, CD40-activated B cell, B cell, antigen presentation, APC, immunotherapy, cancer vaccine
DNA Transfection of Mammalian Skeletal Muscles using In Vivo Electroporation
Department of Physiology, David Geffen School of Medicine, University of California, Los Angeles
Date Published: October 19, 2009 | doi:10.3791/1520 | Section: JoVE General
Keywords: Cellular Biology, electroporation, skeletal muscle, plasmids, protein expression, mouse, two-photon microscopy, fluorescence, transgenic
Noninvasive In Vivo Small Animal MRI and MRS: Basic Experimental Procedures
1Department of Radiology, University of Washington, 2Department of Bioengineering, University of Washington
Date Published: October 20, 2009 | doi:10.3791/1592 | Section: JoVE General
Keywords: Medicine, Small animal, MRI, MRS, mouse, brain, skeletal muscle, tumor, ischemia
Electrospinning Fibrous Polymer Scaffolds for Tissue Engineering and Cell Culture
Department of Bioengineering, University of Pennsylvania
Date Published: October 21, 2009 | doi:10.3791/1589 | Section: JoVE General
Keywords: Cellular Biology, Electrospinning, Photocrosslinking, Photopatterning, Tissue Engineering, Scaffolds, Biomaterials, Bioengineering
Isolation and Derivation of Mouse Embryonic Germinal Cells
Date Published: October 22, 2009 | doi:10.3791/1635 | Section: JoVE General
Keywords: Developmental Biology, Primordial Germinal Cell, Embryonic Germinal Cells, infertility, gonad formation, embryonic development
ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection
Department of Sciences and Chemical Technologies, University of Rome, Tor Vergata
Date Published: October 23, 2009 | doi:10.3791/1588 | Section: JoVE General
Keywords: Biochemistry, Immunosensors, assay, antibody, magnetic bead, electrochemical, screen printed electrodes, array, toxin, food
Cellular Encapsulation in 3D Hydrogels for Tissue Engineering
1Department of Bioengineering, University of Pennsylvania, 2Department of Bioengineering, University of Pennsylvania-School of Medicine
Date Published: October 26, 2009 | doi:10.3791/1590 | Section: JoVE General
Keywords: Cellular Biology, Hydrogel, Tissue Engineering, Biomaterials, Encapsulation, Scaffolds, Bioengineering, Cell Culture, Polymers
Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells
Date Published: October 27, 2009 | doi:10.3791/1644 | Section: JoVE General
Keywords: Medicine, thrombus formation, anti-thrombotic, microfluidic, whole blood assay, IC50, drug screening, platelet, adhesion
Isolation and Large Scale Expansion of Adult Human Endothelial Colony Forming Progenitor Cells
Stem Cell Research Unit, Medical University of Graz, Austria
Date Published: October 28, 2009 | doi:10.3791/1524 | Section: JoVE General
Keywords: Cellular Biology, endothelial colony forming progenitor cells (ECFCs) pooled human platelete lysate (pHPL) large scale expansion, cell culture, isolation, stem cells
Denaturing Urea Polyacrylamide Gel Electrophoresis (Urea PAGE)
1School of Biological Sciences, Nanyang Technological University, Singapore - NTU, 2Singapore-MIT Alliance for Reserach and Technology (SMART)
Date Published: October 29, 2009 | doi:10.3791/1485 | Section: JoVE General
Keywords: Molecular Biology, DNA & RNA analysis, denaturing urea polyacrylamide gel electrophoresis, Protocols
Preparation of Pooled Human Platelet Lysate (pHPL) as an Efficient Supplement for Animal Serum-Free Human Stem Cell Cultures
Stem Cell Research Unit, Medical University of Graz, Austria
Date Published: October 30, 2009 | doi:10.3791/1523 | Section: JoVE General
Keywords: Cellular Biology, Pooled human platelet lysate (pHPL), platelet derived growth factors (PDGFs), cell culture, stem cells
