Table of Contents: Issue 33, November 2009

The 2009 Lindau Nobel Laureate Meeting: Erwin Neher, Physiology or Medicine 1991

JoVE 1563

Erwin Neher

Date Published: November 11, 2009  |  doi:10.3791/1563  |  Section: JoVE Editorial

Keywords: Cellular Biology, electrophysiology, Nobel Prize, Nobel Laureate Meeting, Physiology or Medicine, 1991, single ion channels, patch- clamp

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

JoVE 1542

Nicolas Di Fiori¹, Amit Meller^1,2

¹Physics Department, Boston University, ²Department of Biomedical Engineering, Boston University

Date Published: November 2, 2009  |  doi:10.3791/1542  |  Section: JoVE General

Keywords: Cellular Biology, single molecule FRET, DNA, surface immobilization, microfluidics, confocal microscope

Microvolume Protein Concentration Determination using the NanoDrop 2000c Spectrophotometer

JoVE 1610

Philippe Desjardins, Joel B. Hansen, Michael Allen

Thermo Scientific NanoDrop Products

Date Published: November 4, 2009  |  doi:10.3791/1610  |  Section: JoVE General

Keywords: Basic Protocols, NanoDrop, protein measurement, protein concentration, spectrophotometer, A280, UV/Vis, BCA, microvolume, microsample, proteomics

Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell

JoVE 1530

Andrea Balbo, Huaying Zhao, Patrick H. Brown, Peter Schuck

National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Dynamics of Macromolecular Assembly, Laboratory of Bioengineering and Physical Science

Date Published: November 5, 2009  |  doi:10.3791/1530  |  Section: JoVE General

Keywords: Basic Protocols, analytical ultracentrifugation, sedimentation velocity, sedimentation equilibrium, protein characterization, sedimentation coefficient

Digital Microfluidics for Automated Proteomic Processing

JoVE 1603

Mais J. Jebrail¹, Vivienne N. Luk^1,2, Steve C. C. Shih^2,3, Ryan Fobel^2,3, Alphonsus H. C. Ng^2,3, Hao Yang¹, Sergio L. S. Freire¹, Aaron R. Wheeler^1,2,3

¹Department of Chemistry, University of Toronto, ²Donnelly Centre for Cellular and Biomolecular Research, ³Institute for Biomaterials and Biomedical Engineering, University of Toronto

Date Published: November 6, 2009  |  doi:10.3791/1603  |  Section: JoVE General

Keywords: Bioengineering, digital microfluidics, protein processing, protein extraction, protein precipitation, biochemical assays, reduction, alkylation, digestion, automation, feedback

Historical View and Physiology Demonstration at the NMJ of the Crayfish Opener Muscle

JoVE 1595

Ann S. Cooper, Robin L. Cooper

Department of Biology, University of Kentucky

Date Published: November 9, 2009  |  doi:10.3791/1595  |  Section: JoVE General

Keywords: Cellular Biology, invertebrate, NMJ, synapse, quanta, vesicle

Extraction of High Molecular Weight Genomic DNA from Soils and Sediments

JoVE 1569

Sangwon Lee, Steven J. Hallam

Department of Microbiology and Immunology, University of British Columbia - UBC

Date Published: November 10, 2009  |  doi:10.3791/1569  |  Section: JoVE General

Keywords: Microbiology, Environmental DNA, high molecular weight genomic DNA, DNA extraction, soil, sediments

The use of Biofeedback in Clinical Virtual Reality: The INTREPID Project

JoVE 1554

Claudia Repetto¹, Alessandra Gorini¹, Cinzia Vigna¹, Davide Algeri¹, Federica Pallavicini¹, Giuseppe Riva^1,2

¹Applied Technology for Neuro-Psychology Lab, Istituto Auxologico Italiano, ²Psychology Department, Università Cattolica del Sacro Cuore

Date Published: November 12, 2009  |  doi:10.3791/1554  |  Section: JoVE General

Keywords: Neuroscience, virtual reality, biofeedback, generalized anxiety disorder, Intrepid, cybertherapy, cyberpsychology

Generation of Induced Pluripotent Stem Cells by Reprogramming Mouse Embryonic Fibroblasts with a Four Transcription Factor, Doxycycline Inducible Lentiviral Transduction System

JoVE 1447

Brad Hamilton¹, Qiang Feng¹, Mike Ye¹, G Grant Welstead²

¹Stemgent, ²Whitehead Institute for Biomedical Research, MIT - Massachusetts Institute of Technology

Date Published: November 13, 2009  |  doi:10.3791/1447  |  Section: JoVE General

Keywords: Developmental Biology, reprogramming, Doxycycline, DOX, iPS, induced pluripotent stem cells, lentivirus, pluripotency, transduction, stem cells

Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies

JoVE 1596

Ann S. Cooper, Kylah E. Rymond, Matthew A. Ward, Easter L. Bocook, Robin L. Cooper

Department of Biology, University of Kentucky, Lexington

Date Published: November 16, 2009  |  doi:10.3791/1596  |  Section: JoVE General

Keywords: Cellular Biology, Invertebrate, myocyte, pacemaker, insect

Dissection of Hippocampal Dentate Gyrus from Adult Mouse

JoVE 1543

Hideo Hagihara^1,2, Keiko Toyama^1,2, Nobuyuki Yamasaki^1,3, Tsuyoshi Miyakawa^1,2,4,5

¹Japan Science and Technology Agency, Core Research for Evolutionary Science and Technology (CREST), ²Division of Systems Medical Science, Institute for Comprehensive Medical Science, Fujita Health University, ³Department of Psychiatry, Graduate School of Medicine, Kyoto University, ⁴Genetic Engineering and Functional Genomics Group, Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, ⁵Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, National Institutes of Natural Sciences

Date Published: November 17, 2009  |  doi:10.3791/1543  |  Section: JoVE General

Keywords: Neuroscience, Dentate gyrus, Hippocampus, dissection, neurogenesis, alpha-CaMKII, mouse

The use of SC1 (Pluripotin) to Support mESC Self-renewal in the Absence of LIF

JoVE 1550

Wen Xiong¹, Yan Gao¹, Xun Cheng¹, Charles Martin¹, Dongmei Wu¹, Shuyuan Yao¹, Min-Ju Kim², Yang Liu¹

¹Research and Development, Stemgent, ²Product Marketing, Stemgent

Date Published: November 18, 2009  |  doi:10.3791/1550  |  Section: JoVE General

Keywords: Cellular Biology, SC1(Pluripotin), LIF, mESC, mouse ESC, mouse ES cells, pluripotency, self-renewal, small molecule

Measuring the Induced Membrane Voltage with Di-8-ANEPPS

JoVE 1659

Gorazd Pucihar, Tadej Kotnik, Damijan Miklavčič

Department of Biomedical Engineering, Faculty of Electrical Engineering, University of Ljubljana

Date Published: November 19, 2009  |  doi:10.3791/1659  |  Section: JoVE General

Keywords: Cellular Biology, induced transmembrane voltage, induced membrane voltage, induced transmembrane potential, potentiometric dyes, di-8-ANEPPS, electroporation, electropermeabilization

Combining Peripheral Nerve Grafting and Matrix Modulation to Repair the Injured Rat Spinal Cord

JoVE 1324

John D. Houle, Arthi Amin, Marie-Pascale Cote, Michel Lemay, Kassi Miller, Harra Sandrow, Lauren Santi, Jed Shumsky, Veronica Tom

Department of Neurobiology and Anatomy, Drexel University College of Medicine

Date Published: November 20, 2009  |  doi:10.3791/1324  |  Section: JoVE General

Keywords: Neurobiology, transplantation, SCI, regeneration, tract tracing, electrophysiology

Methods for the Study of the Zebrafish Maxillary Barbel

JoVE 1558

Elizabeth E. LeClair¹, Jacek Topczewski²

¹Department of Biological Sciences, DePaul University, ²Children’s Memorial Research Center, Department of Pediatrics, Northwestern University Feinberg School of Medicine

Date Published: November 23, 2009  |  doi:10.3791/1558  |  Section: JoVE General

Keywords: Developmental Biology, zebrafish, regeneration, barbel, surgery, vasculature, circulation, imaging, agar, embedding, microscopy

Aortic Ring Assay

JoVE 1564

Keren Bellacen, Eli C. Lewis

Department Clinical Biochemistry, Ben-Gurion University

Date Published: November 24, 2009  |  doi:10.3791/1564  |  Section: JoVE General

Keywords: Medicine, aortic rings, angiogenesis, blood vessels, aorta, mouse, vessel outgrowth

Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging

JoVE 1581

Melissa Paulite¹, Zahra Fakhraai², Boris B. Akhremitchev³, Kerstin Mueller¹, Gilbert C. Walker¹

¹Department of Chemistry, University of Toronto, ²Department of Chemistry, University of Wisconsin, ³Department of Chemistry, Duke University

Date Published: November 25, 2009  |  doi:10.3791/1581  |  Section: JoVE General

Keywords: Cellular Biology, nearfield imaging, infrared, amyloid, fibril, protein

Chick ex ovo Culture and ex ovo CAM Assay: How it Really Works

JoVE 1620

Daniel S. Dohle¹, Susanne D. Pasa¹, Sebastian Gustmann², Markus Laub³, Josef H. Wissler⁴, Herbert P. Jennissen¹, Nicole Dünker²

¹Institute for Physiological Chemistry, Department of Biochemical Endocrinology, University of Duisburg-Essen, ²Institute for Anatomy, Department of Neuroanatomy, University of Duisburg-Essen, ³Morphoplant GmbH, ⁴ARCONS Institute for Applied Research and Didactics

Date Published: November 30, 2009  |  doi:10.3791/1620  |  Section: JoVE General

Keywords: Developmental Biology, chick, ex ovo culture, in ovo culture, chorioallantoic membrane, CAM, angiogenesis, tumor, limb buds; grafting

Obtaining Highly Purified Toxoplasma gondii Oocysts by a Discontinuous Cesium Chloride Gradient

JoVE 1420

Sarah E. Staggs¹, Mary Jean See², J P. Dubey³, Eric N. Villegas^2,4

¹Dynamac, Inc., ²Department of Biological Sciences, University of Cincinnati, McMicken College of Arts and Science, ³Animal Parasitic Disease Laboratory, Agricultural Research Service, U.S. Department of Agriculture, ⁴National Exposure Research Laboratory, US Environmental Protection Agency

Date Published: November 3, 2009  |  doi:10.3791/1420  |  Section: JoVE Immunology and Infection

Keywords: Jove Infectious Diseases, Microbiology, Toxoplasma gondii, cesium chloride, oocysts, discontinuous gradient, apicomplexan