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The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.

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Table of Contents

Table of Contents: Issue 55, September 2011

Jump to:  JoVE Editorial (1) JoVE General (16) JoVE Neuroscience (10) JoVE Immunology and Infection (11) JoVE Clinical and Translational Medicine (9) JoVE Bioengineering (7)
 JoVE General

Preparation of Quality Inositol Pyrophosphates


JoVE 3027

, , , ,

Medical Research Council (MRC), Cell Biology Unit and Laboratory for Molecular Cell Biology, University College London

Date Published: September 3, 2011  |  doi:10.3791/3027  |  Section: JoVE General

Keywords: Molecular Biology, Polyacrilamyde Gel Electrophoresis (PAGE), inositol hexakisphosphate (IP6), phytic acid, diphosphoinositol pentakisphosphate (IP7), bisdiphoshoinositol tetrakisphosphate (IP8), IP6-kinase (IP6K), PP-IP5K, VIP1

 JoVE General

Mutagenesis and Analysis of Genetic Mutations in the GC-rich KISS1 Receptor Sequence Identified in Humans with Reproductive Disorders


JoVE 2897

1, 1, 1,2

1Division of Endocrinology and Metabolism, Department of Medicine, University of Miami Miller School of Medicine, 2Department of Molecular and Cellular Pharmacology, University of Miami Miller School of Medicine

Date Published: September 4, 2011  |  doi:10.3791/2897  |  Section: JoVE General

Keywords: Genetics, GPR54, KISS1R, precocious puberty, membrane receptor, proteasome, degradation, GC-rich, site-directed mutagenesis, immunoprecipitation

 JoVE General

A Matrigel-Based Tube Formation Assay to Assess the Vasculogenic Activity of Tumor Cells


JoVE 3040

1, 1, 1,2,3

1Molecular and Cellular Biology Program, Morrill Science Center, University of Massachusetts, 2Pioneer Valley Life Sciences Institute, University of Massachusetts, 3Department of Veterinary and Animal Sciences, University of Massachusetts

Date Published: September 7, 2011  |  doi:10.3791/3040  |  Section: JoVE General

Keywords: Cancer Biology, tumor, vascular, endothelial, tube formation, Matrigel, in vitro

 JoVE General

Bromodeoxyuridine (BrdU) Labeling and Subsequent Fluorescence Activated Cell Sorting for Culture-independent Identification of Dissolved Organic Carbon-degrading Bacterioplankton


JoVE 2855

*1, *1, 1, 2, 1

1Biological Sciences, Kent State University, 2Marine Sciences, University of Georgia (UGA)

Date Published: September 10, 2011  |  doi:10.3791/2855  |  Section: JoVE General

Keywords: Molecular Biology, BrdU incorporation, fluorescence-activated cell sorting, FACS, flow cytometry, microbial community, culture-independent, bacterioplankton

 JoVE General

Biophysical Assays to Probe the Mechanical Properties of the Interphase Cell Nucleus: Substrate Strain Application and Microneedle Manipulation


JoVE 3087

1, 1, 1,2

1Brigham and Women's Hospital / Harvard Medical School, Department of Medicine, Cardiovascular Division, 2Weill Institute for Cell and Molecular Biology & Department of Biomedical Engineering, Cornell University

Date Published: September 14, 2011  |  doi:10.3791/3087  |  Section: JoVE General

Keywords: Biophysics, nuclear envelope, nuclear stiffness, nucleo-cytoskeletal coupling, lamin, nesprin, cytoskeleton, biomechanics, nuclear deformation, force transmission

 JoVE General

Detection of Protein Interactions in Plant using a Gateway Compatible Bimolecular Fluorescence Complementation (BiFC) System


JoVE 3473

1, 2, 2, 1, 2

1Department of Biology, University of Western Ontario, 2Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada

Date Published: September 16, 2011  |  doi:10.3791/3473  |  Section: JoVE General

Keywords: Plant Biology, protein interaction, Gateway, Bimolecular fluorescence complementation, Confocal microscope, Agrobacterium, Nicotiana benthamiana, Arabidopsis

 JoVE General

Imaging G-protein Coupled Receptor (GPCR)-mediated Signaling Events that Control Chemotaxis of Dictyostelium Discoideum


JoVE 3128

,

Chemotaxis Signal Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health

Date Published: September 20, 2011  |  doi:10.3791/3128  |  Section: JoVE General

Keywords: Molecular Biology, Chemotaxis, directional sensing, GPCR, PCR, G-proteins, signal transduction, Dictyostelium discoideum

 JoVE General

Biochemical Reconstitution of Steroid Receptor•Hsp90 Protein Complexes and Reactivation of Ligand Binding


JoVE 3059

1, 2, 3

1College of Nursing, Interdisciplinary Life Sciences Research Laboratory, Seattle University, 2College of Science and Engineering, Interdisciplinary Life Sciences Research Laboratory, Seattle University, 3School of Medicine, University of Washington

Date Published: September 21, 2011  |  doi:10.3791/3059  |  Section: JoVE General

Keywords: Biochemistry, glucocorticoid receptor, hsp90, molecular chaperone protein, in vitro reconstitution, steroid binding, biochemistry, immunoadsorption, immunoprecipitation, Experion, western blot

 JoVE General

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification


JoVE 3060

1, 2, 1

1College of Nursing, Interdisciplinary Life Sciences Research Laboratory, Seattle University, 2College of Science and Engineering, Interdisciplinary Life Sciences Research Laboratory, Seattle University

Date Published: September 21, 2011  |  doi:10.3791/3060  |  Section: JoVE General

Keywords: Biochemistry, hydrophobic interaction chromatography, liquid chromatography, green fluorescent protein, GFP, scouting, protein purification, Bio-Rad DuoFlow, FPLC

 JoVE General

Detection of Histone Modifications in Plant Leaves


JoVE 3096

1,2, 3, 2

1Department of Botany, RWTH Aachen University, 2Department of Plant Physiology, RWTH Aachen University, 3Department of Botany, Leibniz University

Date Published: September 23, 2011  |  doi:10.3791/3096  |  Section: JoVE General

Keywords: Molecular Biology, chromatin, chromatin immunoprecipitation, ChIP, histone modifications, PCR, plant molecular biology, plant promoter control, gene regulation

 JoVE General

Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae


JoVE 3150

1,2, 2, 3, 2

1Irell & Manella Graduate School of Biological Sciences, 2Department of Molecular and Cellular Biology, City of Hope Comprehensive Cancer Center and Beckman Research Institute, 3Department of Biochemistry and Molecular Biology, University of Southern California, Norris Comprehensive Cancer Center

Date Published: September 23, 2011  |  doi:10.3791/3150  |  Section: JoVE General

Keywords: Genetics, translocation formation, HO-endonuclease, Genomic Southern blot, Chromosome blot, Pulsed-field gel electrophoresis, Homologous recombination, DNA double-strand breaks, Single-strand annealing

 JoVE General

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells


JoVE 3343

, ,

SwitchGear Genomics

Date Published: September 28, 2011  |  doi:10.3791/3343  |  Section: JoVE General

Keywords: Genetics, MicroRNA, miRNA, mimic, Clone, 3' UTR, Assay, vector, LightSwitch, luciferase, co-transfection, 3'UTR REPORTER, mirna target, microrna target, reporter, GoClone, Reporter construct

 JoVE General

Studying Age-dependent Genomic Instability using the S. cerevisiae Chronological Lifespan Model


JoVE 3030

, ,

Andrus Gerontology Center, Department of Biological Sciences, Department of Molecular and Computational Biology, University of Southern California, Los Angeles

Date Published: September 29, 2011  |  doi:10.3791/3030  |  Section: JoVE General

Keywords: Genetics, saccharomyces cerevisiae, life span, aging, mutation frequency, genomic instability

 JoVE Neuroscience

Live Imaging of Dorsal Root Axons after Rhizotomy


JoVE 3126

1, 2,3, 4

1Temple University, Shriners Hospitals Pediatric Research Center and Department of Anatomy and Cell Biology, 2Medical Research Service, Department of Veterans Affairs Hospital, 3Department of Neurobiology and Anatomy, Drexel University College of Medicine, 4Shriners Hospitals Pediatric Research Center and Department of Anatomy and Cell Biology, Temple University School of Medicine

Date Published: September 1, 2011  |  doi:10.3791/3126  |  Section: JoVE Neuroscience

Keywords: Neuroscience, in vivo imaging, dorsal root injury, wide field fluorescence microscope, laminectomy, spinal cord, Green fluorescence protein, transgenic mice, dorsal root ganglion, spinal root injury

 JoVE Neuroscience

Examining Local Network Processing using Multi-contact Laminar Electrode Recording


JoVE 2806

1, 1, 2

1Graduate School of Biomedical Science, Neuroscience Program, University of Texas, 2Department of Neurobiology and Anatomy, University of Texas

Date Published: September 8, 2011  |  doi:10.3791/2806  |  Section: JoVE Neuroscience

Keywords: Neuroscience, laminar probes, cortical layers, local-field potentials, population coding

 JoVE Neuroscience

Subcutaneous Administration of Muscarinic Antagonists and Triple-Immunostaining of the Levator Auris Longus Muscle in Mice


JoVE 3124

1, 2, 3

1Biology Department, Arcadia University, 2Shriners Hospitals Pediatric Research Center, Temple University School of Medicine, 3Shriners Hospitals Pediatric Research Center and Department of Anatomy and Cell Biology, Temple University School of Medicine

Date Published: September 8, 2011  |  doi:10.3791/3124  |  Section: JoVE Neuroscience

Keywords: Neuroscience, neuromuscular junction, immunohistochemistry, muscle, Schwann cells, acetylcholine receptors, confocal microscope

 JoVE Neuroscience

Lectin-based Isolation and Culture of Mouse Embryonic Motoneurons


JoVE 3200

1, 2, 1, 2, 1, 1

1Institute for Cellmorphology and molecular Neurobiology, Group for Cellbiology, Ruhr-University Bochum, 2Institute for Clinical Neurobiology, University of Wuerzburg

Date Published: September 15, 2011  |  doi:10.3791/3200  |  Section: JoVE Neuroscience

Keywords: Neuroscience, p75NTR, spinal cord, lectin, axon, dendrite

 JoVE Neuroscience

Preparation of Synaptoneurosomes from Mouse Cortex using a Discontinuous Percoll-Sucrose Density Gradient


JoVE 3196

1, 1, 2, 1

1Department of Pathology and Laboratory Medicine, Waisman Center for Developmental Disabilities, University of Wisconsin, 2Department of Biochemistry, Waisman Center for Developmental Disabilities, University of Wisconsin

Date Published: September 17, 2011  |  doi:10.3791/3196  |  Section: JoVE Neuroscience

Keywords: Neuroscience, synaptoneurosomes, synaptosomes, Percoll-sucrose gradients, neurons, synapse, cortex, mouse

 JoVE Neuroscience

Using a Comparative Species Approach to Investigate the Neurobiology of Paternal Responses


JoVE 3173

1, 2, 1

1Department of Psychology, Randolph-Macon College, 2Department of Psychology, Marshall University

Date Published: September 19, 2011  |  doi:10.3791/3173  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Peromyscus, mouse, paternal behavior, vasopressin, immunocytochemistry, microsequencing behavioral analysis

 JoVE Neuroscience

Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory Neurons


JoVE 3195

1,2, 1, 1,3

1SISSA, International School for Advanced Studies, 2Istituto di Biofisica, Consiglio Nazionale delle Ricerche, 3SISSA Unit, Italian Institute of Technology

Date Published: October 29, 2011  |  doi:10.3791/3195  |  Section: JoVE Neuroscience

Keywords: Neuroscience, caged compounds, caged cAMP, caged Ca, olfactory sensory neuron, olfaction, whole-cell patch-clamp, flash photolysis, flash lampc

 JoVE Immunology and Infection

Diagnosis of Ecto- and Endoparasites in Laboratory Rats and Mice


JoVE 2767

1, 1, 1, 1, 1, 2,3

1Research Animal Diagnostic Services, Charles River, 2Research Models and Services, Charles River, 3Department of Comparative Medicine, University of Washington

Date Published: September 6, 2011  |  doi:10.3791/2767  |  Section: JoVE Immunology and Infection

Keywords: Immunology, rat, mouse, endoparasite, ectoparasite, diagnostics, mites, pinworm, helminths, protozoa, health monitoring

 JoVE Immunology and Infection

Expansion of Human Peripheral Blood γδ T Cells using Zoledronate


JoVE 3182

1,2, 1,2, 1,2, 1,2, 1,2, 1, 1

1Department of Immunotherapeutics (Medinet), University of Tokyo Hospital, 2MEDINET Co., Ltd

Date Published: September 9, 2011  |  doi:10.3791/3182  |  Section: JoVE Immunology and Infection

Keywords: Immunology, γδ T Cell, zoledronate, PBMC, peripheral blood mononuclear cells

 JoVE Immunology and Infection

A Functional Whole Blood Assay to Measure Viability of Mycobacteria, using Reporter-Gene Tagged BCG or M.Tb (BCG lux/M.Tb lux)


JoVE 3332

1, 2, 1

1Department of Paediatrics, Imperial College London, 2Centre for Health Sciences, Barts & The London School of Medicine and Dentistry

Date Published: September 14, 2011  |  doi:10.3791/3332  |  Section: JoVE Immunology and Infection

Keywords: Immunology, M.tuberculosis, BCG, whole blood assay, lux reporter genes, immune responses, tuberculosis, host pathogen interactions

 JoVE Immunology and Infection

Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity


JoVE 3263

1, 2, 3, 4, 5, 3, 1

1Department of Molecular Biology, University of Salzburg, 2Department of Neurology, Paracelsus Medical University, 3Department of Dermatology, Paracelsus Medical University, 4Bühlmann Laboratories, 5Christian Doppler Laboratory for Allergy Diagnosis and Therapy, University of Salzburg

Date Published: September 16, 2011  |  doi:10.3791/3263  |  Section: JoVE Immunology and Infection

Keywords: Immunology, NSAIDs, hypersensitivity, propyphenazone, diclofenac, drug conjugates, basophil activation test

 JoVE Immunology and Infection

Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing


JoVE 3322

1, 1, 2, 3, 4, 1

1School of Molecular Bioscience, University of Sydney, 2Department of Surgery, Royal Prince Alfred Hospital, 3Department of Anatomical Pathology, Department of Anatomical Pathology, 4Department of Medicine, Concord Repatriation General Hospital

Date Published: September 25, 2011  |  doi:10.3791/3322  |  Section: JoVE Immunology and Infection

Keywords: Immunology, colorectal cancer, leukocytes, antibody microarray, multiplexing, fluorescence, CD antigens

 JoVE Immunology and Infection

Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays


JoVE 2960

1, 1, 1, 1, 1, 2, 3

1The virology Core at the HIV Drug Resistance Program, NCI-Frederick, 2Division of Infectious Diseases, University of Pittsburgh, 3Department of Molecular Biology and Microbiology, Tuffts University

Date Published: September 26, 2011  |  doi:10.3791/2960  |  Section: JoVE Immunology and Infection

Keywords: Immunology, single genome sequencing, SGS, real-time PCR, single-copy assay, SCA, HIV-1, ultra-sensitive, RNA extraction

 JoVE Immunology and Infection

RNA Isolation of Pseudomonas aeruginosa Colonizing the Murine Gastrointestinal Tract


JoVE 3293

1, 1, 2, 3

1Department of Pediatrics, University of Texas Southwestern Medical Center, 2Channing Laboratory, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 3Department of Pediatrics and Microbiology, University of Texas Southwestern Medical Center

Date Published: September 28, 2011  |  doi:10.3791/3293  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Pseudomonas, RNA, murine, cecum, transcriptome, qPCR, RT-PCR, PCR

 JoVE Immunology and Infection

Separation of Single-stranded DNA, Double-stranded DNA and RNA from an Environmental Viral Community Using Hydroxyapatite Chromatography


JoVE 3146

1, 2, 1

1Department of Microbial and Environmental Genomics, The J. Craig Venter Institute, 2Department of Synthetic Biology and Bioenergy, The J. Craig Venter Institute

Date Published: September 29, 2011  |  doi:10.3791/3146  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Hydroxyapatite, single-stranded DNA, double-stranded DNA, RNA, DNA, chromatography, viral ecology, virus, bacteriophage

 JoVE Clinical and Translational Medicine

Guide Wire Assisted Catheterization and Colored Dye Injection for Vascular Mapping of Monochorionic Twin Placentas


JoVE 2837

1, 1, 1, 1, 1, 2, 3, 4

1Division of Pediatric and Fetal Surgery, Department of Surgery, University of California, San Francisco, 2Department of Pathology, University of Alberta, 3Department of Obstretics and Gynecology, University of California, San Francisco, 4Department of Radiology, University of California, San Francisco

Date Published: September 5, 2011  |  doi:10.3791/2837  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, placenta, monochorionic twins, vascular mapping, twin-to-twin transfusion syndrome (TTTS), obstetrics, fetal surgery

 JoVE Clinical and Translational Medicine

Accurate and Simple Evaluation of Vascular Anastomoses in Monochorionic Placenta using Colored Dye


JoVE 3208

1, 2, 2, 2, 3, 1, 2

1Division of Neonatology, Department of Pediatrics, Leiden University Medical Center, 2Division of Fetal Therapy, Department of Obstetrics, Leiden University Medical Center, 3Department of Obstetrics, Leiden University Medical Center

Date Published: September 5, 2011  |  doi:10.3791/3208  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, monochorionic twin placenta, vascular anastomoses, twin-to-twin transfusion syndrome, twin anemia polycythemia sequence, colored dye injection, fetoscopic laser surgery

 JoVE Clinical and Translational Medicine

Probing the Brain in Autism Using fMRI and Diffusion Tensor Imaging


JoVE 3178

, , , , , ,

Department of Psychology, University of Alabama at Birmingham

Date Published: September 12, 2011  |  doi:10.3791/3178  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Functional magnetic resonance imaging (fMRI), MRI, Diffusion tensor imaging (DTI), Functional Connectivity, Neuroscience, Developmental disorders, Autism, Fractional Anisotropy

 JoVE Clinical and Translational Medicine

Changing the Direction and Orientation of Electric Field During Electric Pulses Application Improves Plasmid Gene Transfer in vitro


JoVE 3309

1, 2, 2, 2, 2

1Department of Fundamentals of Electrical Engineering, Mathematics and Physics, University of Ljubljana, 2Department of Biomedical Engineering, University of Ljubljana

Date Published: September 12, 2011  |  doi:10.3791/3309  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, gene electrotransfer, GFP, changing the orientation of electric field, plasmid, gene, transfection

 JoVE Clinical and Translational Medicine

Intraspinal Cell Transplantation for Targeting Cervical Ventral Horn in Amyotrophic Lateral Sclerosis and Traumatic Spinal Cord Injury


JoVE 3069

Department of Neuroscience, Thomas Jefferson University Medical College

Date Published: September 18, 2011  |  doi:10.3791/3069  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, cell transplantation, engraftment, graft, spinal cord, stem cells, precursors, ALS, amyotrophic lateral sclerosis, motor neuron, SCI, spinal cord injury

 JoVE Clinical and Translational Medicine

A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics


JoVE 3010

, , , , , ,

Merck Research Laboratory, Merck & Co., Inc

Date Published: September 22, 2011  |  doi:10.3791/3010  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Manufacturing cell line, protein therapeutics, automation, high-throughput, FACS, FACS Aria, CloneSelect Imager, TECAN Freedom EVO liquid handling system

 JoVE Clinical and Translational Medicine

Mesenteric Artery Contraction and Relaxation Studies Using Automated Wire Myography


JoVE 3119

1, 1, 1,2,3, 1,2,3

1Julius L. Chambers Biomedical/Biotechnology Research Institute, North Carolina Central University, Durham, 2Department of Biology, North Carolina Central University, Durham, 3Department of Physiology & Pharmacology and Hypertension & Vascular Research Center, Wake Forest University School of Medicine

Date Published: September 22, 2011  |  doi:10.3791/3119  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, cardiovascular, resistant arteries, contraction, relaxation, myography

 JoVE Bioengineering

Autologous Endothelial Progenitor Cell-Seeding Technology and Biocompatibility Testing For Cardiovascular Devices in Large Animal Model


JoVE 3197

1, 2, 3, 1, 1, 4, 3, 1, 3

1Department of Biomedical Engineering, Duke University, 2School of Medicine, Duke University, 3Department of Surgery, Duke University Medical Center, 4School of Medicine, University of Pennsylvania

Date Published: September 9, 2011  |  doi:10.3791/3197  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Stent, Titanium, Thrombosis, Endothelial Progenitor Cell, Endothelium, Biomaterial, Biocompatibility, Bioengineering, Translational Medicine, Vascular Surgery, Porcine

 JoVE Bioengineering

Formulation of Diblock Polymeric Nanoparticles through Nanoprecipitation Technique


JoVE 3398

1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2

1Laboratory of Nano- and Translational Medicine, Department of Radiation Oncology, Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, 2Carolina Center for Nanotechnology Excellence, University of North Carolina

Date Published: September 20, 2011  |  doi:10.3791/3398  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Nanoparticles, nanomedicine, drug delivery, polymeric micelles, polymeric nanoparticles, diblock co-polymers, nanoplatform, nanoparticle molecular imaging, polymer conjugation.

 JoVE Bioengineering

Utilization of Plasmonic and Photonic Crystal Nanostructures for Enhanced Micro- and Nanoparticle Manipulation


JoVE 3390

1, 2,3, 2,4,5, 1

1Electrical Engineering Department, University of Washington, 2Division of Human Biology, Fred Hutchinson Cancer Research Center, 3Molecular and Cellular Biology Program, University of Washington, 4Clinical Research, Fred Hutchinson Cancer Research Center, 5Public Health Sciences, Fred Hutchinson Cancer Research Center

Date Published: September 27, 2011  |  doi:10.3791/3390  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Surface plasmon, optical trapping, optical tweezers, plasmonic trapping, cell manipulation, optical manipulation

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