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General Laboratory Techniques

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Basic Methods in Cellular and Molecular Biology

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Model Organisms I

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Table of Contents

Table of Contents: Issue 55, September 2011

Jump to:  JoVE Editorial (1) JoVE Biology (16) JoVE Neuroscience (10) JoVE Immunology and Infection (11) JoVE Clinical and Translational Medicine (9) JoVE Bioengineering (7)
 JoVE Biology

Preparation of Quality Inositol Pyrophosphates

1Medical Research Council (MRC), Cell Biology Unit and Laboratory for Molecular Cell Biology, University College London


JoVE 3027

Date Published: September 3, 2011  |  doi:10.3791/3027  |  Section: JoVE Biology

 JoVE Biology

Mutagenesis and Analysis of Genetic Mutations in the GC-rich KISS1 Receptor Sequence Identified in Humans with Reproductive Disorders

1Division of Endocrinology and Metabolism, Department of Medicine, University of Miami Miller School of Medicine, 2Department of Molecular and Cellular Pharmacology, University of Miami Miller School of Medicine


JoVE 2897

Date Published: September 4, 2011  |  doi:10.3791/2897  |  Section: JoVE Biology

 JoVE Biology

A Matrigel-Based Tube Formation Assay to Assess the Vasculogenic Activity of Tumor Cells

1Molecular and Cellular Biology Program, Morrill Science Center, University of Massachusetts, 2Pioneer Valley Life Sciences Institute, University of Massachusetts, 3Department of Veterinary and Animal Sciences, University of Massachusetts


JoVE 3040

Date Published: September 7, 2011  |  doi:10.3791/3040  |  Section: JoVE Biology

 JoVE Biology

Bromodeoxyuridine (BrdU) Labeling and Subsequent Fluorescence Activated Cell Sorting for Culture-independent Identification of Dissolved Organic Carbon-degrading Bacterioplankton

1Biological Sciences, Kent State University, 2Marine Sciences, University of Georgia (UGA)


JoVE 2855

Date Published: September 10, 2011  |  doi:10.3791/2855  |  Section: JoVE Biology

 JoVE Biology

Biophysical Assays to Probe the Mechanical Properties of the Interphase Cell Nucleus: Substrate Strain Application and Microneedle Manipulation

1Brigham and Women's Hospital / Harvard Medical School, Department of Medicine, Cardiovascular Division, 2Weill Institute for Cell and Molecular Biology & Department of Biomedical Engineering, Cornell University


JoVE 3087

Date Published: September 14, 2011  |  doi:10.3791/3087  |  Section: JoVE Biology

 JoVE Biology

Detection of Protein Interactions in Plant using a Gateway Compatible Bimolecular Fluorescence Complementation (BiFC) System

1Department of Biology, University of Western Ontario, 2Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada


JoVE 3473

Date Published: September 16, 2011  |  doi:10.3791/3473  |  Section: JoVE Biology

 JoVE Biology

Imaging G-protein Coupled Receptor (GPCR)-mediated Signaling Events that Control Chemotaxis of Dictyostelium Discoideum

1Chemotaxis Signal Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health


JoVE 3128

Date Published: September 20, 2011  |  doi:10.3791/3128  |  Section: JoVE Biology

 JoVE Biology

Biochemical Reconstitution of Steroid Receptor•Hsp90 Protein Complexes and Reactivation of Ligand Binding

1College of Nursing, Interdisciplinary Life Sciences Research Laboratory, Seattle University, 2College of Science and Engineering, Interdisciplinary Life Sciences Research Laboratory, Seattle University, 3School of Medicine, University of Washington


JoVE 3059

Date Published: September 21, 2011  |  doi:10.3791/3059  |  Section: JoVE Biology

 JoVE Biology

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

1College of Nursing, Interdisciplinary Life Sciences Research Laboratory, Seattle University, 2College of Science and Engineering, Interdisciplinary Life Sciences Research Laboratory, Seattle University


JoVE 3060

Date Published: September 21, 2011  |  doi:10.3791/3060  |  Section: JoVE Biology

 JoVE Biology

Detection of Histone Modifications in Plant Leaves

1Department of Botany, RWTH Aachen University, 2Department of Plant Physiology, RWTH Aachen University, 3Department of Botany, Leibniz University


JoVE 3096

Date Published: September 23, 2011  |  doi:10.3791/3096  |  Section: JoVE Biology

 JoVE Biology

Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae

1Irell & Manella Graduate School of Biological Sciences, 2Department of Molecular and Cellular Biology, City of Hope Comprehensive Cancer Center and Beckman Research Institute, 3Department of Biochemistry and Molecular Biology, University of Southern California, Norris Comprehensive Cancer Center


JoVE 3150

Date Published: September 23, 2011  |  doi:10.3791/3150  |  Section: JoVE Biology

 JoVE Biology

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells

1SwitchGear Genomics


JoVE 3343

Date Published: September 28, 2011  |  doi:10.3791/3343  |  Section: JoVE Biology

 JoVE Biology

Studying Age-dependent Genomic Instability using the S. cerevisiae Chronological Lifespan Model

1Andrus Gerontology Center, Department of Biological Sciences, Department of Molecular and Computational Biology, University of Southern California, Los Angeles


JoVE 3030

Date Published: September 29, 2011  |  doi:10.3791/3030  |  Section: JoVE Biology

 JoVE Neuroscience

Live Imaging of Dorsal Root Axons after Rhizotomy

1Temple University, Shriners Hospitals Pediatric Research Center and Department of Anatomy and Cell Biology, 2Medical Research Service, Department of Veterans Affairs Hospital, 3Department of Neurobiology and Anatomy, Drexel University College of Medicine, 4Shriners Hospitals Pediatric Research Center and Department of Anatomy and Cell Biology, Temple University School of Medicine


JoVE 3126

Date Published: September 1, 2011  |  doi:10.3791/3126  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Habituation and Prepulse Inhibition of Acoustic Startle in Rodents

1Anatomy and Cell Biology, Schulich School of Medicine and Dentistry, University of Western Ontario


JoVE 3446

Date Published: September 1, 2011  |  doi:10.3791/3446  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Examining Local Network Processing using Multi-contact Laminar Electrode Recording

1Graduate School of Biomedical Science, Neuroscience Program, University of Texas, 2Department of Neurobiology and Anatomy, University of Texas


JoVE 2806

Date Published: September 8, 2011  |  doi:10.3791/2806  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Subcutaneous Administration of Muscarinic Antagonists and Triple-Immunostaining of the Levator Auris Longus Muscle in Mice

1Biology Department, Arcadia University, 2Shriners Hospitals Pediatric Research Center, Temple University School of Medicine, 3Shriners Hospitals Pediatric Research Center and Department of Anatomy and Cell Biology, Temple University School of Medicine


JoVE 3124

Date Published: September 8, 2011  |  doi:10.3791/3124  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Preparing Undercut Model of Posttraumatic Epileptogenesis in Rodents

1Department of Anatomy and Cell Biology, Department of Neurosurgery, Stark Neuroscience Research Institute, Indiana University School of Medicine


JoVE 2840

Date Published: September 15, 2011  |  doi:10.3791/2840  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Lectin-based Isolation and Culture of Mouse Embryonic Motoneurons

1Institute for Cellmorphology and molecular Neurobiology, Group for Cellbiology, Ruhr-University Bochum, 2Institute for Clinical Neurobiology, University of Wuerzburg


JoVE 3200

Date Published: September 15, 2011  |  doi:10.3791/3200  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Preparation of Synaptoneurosomes from Mouse Cortex using a Discontinuous Percoll-Sucrose Density Gradient

1Department of Pathology and Laboratory Medicine, Waisman Center for Developmental Disabilities, University of Wisconsin, 2Department of Biochemistry, Waisman Center for Developmental Disabilities, University of Wisconsin


JoVE 3196

Date Published: September 17, 2011  |  doi:10.3791/3196  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Using a Comparative Species Approach to Investigate the Neurobiology of Paternal Responses

1Department of Psychology, Randolph-Macon College, 2Department of Psychology, Marshall University


JoVE 3173

Date Published: September 19, 2011  |  doi:10.3791/3173  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory Neurons

1SISSA, International School for Advanced Studies, 2Istituto di Biofisica, Consiglio Nazionale delle Ricerche, 3SISSA Unit, Italian Institute of Technology


JoVE 3195

Date Published: October 29, 2011  |  doi:10.3791/3195  |  Section: JoVE Neuroscience

 JoVE Immunology and Infection

Introducing Shear Stress in the Study of Bacterial Adhesion

1Blood vessels as a target for infection, Paris center for cardiovascular research, INSERM U970


JoVE 3241

Date Published: September 2, 2011  |  doi:10.3791/3241  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Use of the EpiAirway Model for Characterizing Long-term Host-pathogen Interactions

1Division of Basic Medical Sciences, Mercer University School of Medicine


JoVE 3261

Date Published: September 2, 2011  |  doi:10.3791/3261  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Diagnosis of Ecto- and Endoparasites in Laboratory Rats and Mice

1Research Animal Diagnostic Services, Charles River, 2Research Models and Services, Charles River, 3Department of Comparative Medicine, University of Washington


JoVE 2767

Date Published: September 6, 2011  |  doi:10.3791/2767  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Expansion of Human Peripheral Blood γδ T Cells using Zoledronate

1Department of Immunotherapeutics (Medinet), University of Tokyo Hospital, 2MEDINET Co., Ltd


JoVE 3182

Date Published: September 9, 2011  |  doi:10.3791/3182  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

A Functional Whole Blood Assay to Measure Viability of Mycobacteria, using Reporter-Gene Tagged BCG or M.Tb (BCG lux/M.Tb lux)

1Department of Paediatrics, Imperial College London, 2Centre for Health Sciences, Barts & The London School of Medicine and Dentistry


JoVE 3332

Date Published: September 14, 2011  |  doi:10.3791/3332  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity

1Department of Molecular Biology, University of Salzburg, 2Department of Neurology, Paracelsus Medical University, 3Department of Dermatology, Paracelsus Medical University, 4Bühlmann Laboratories, 5Christian Doppler Laboratory for Allergy Diagnosis and Therapy, University of Salzburg


JoVE 3263

Date Published: September 16, 2011  |  doi:10.3791/3263  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Tracking Neutrophil Intraluminal Crawling, Transendothelial Migration and Chemotaxis in Tissue by Intravital Video Microscopy

1Department of Pharmacology, University of Saskatchewan


JoVE 3296

Date Published: September 24, 2011  |  doi:10.3791/3296  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing

1School of Molecular Bioscience, University of Sydney, 2Department of Surgery, Royal Prince Alfred Hospital, 3Department of Anatomical Pathology, Department of Anatomical Pathology, 4Department of Medicine, Concord Repatriation General Hospital


JoVE 3322

Date Published: September 25, 2011  |  doi:10.3791/3322  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays

1The virology Core at the HIV Drug Resistance Program, NCI-Frederick, 2Division of Infectious Diseases, University of Pittsburgh, 3Department of Molecular Biology and Microbiology, Tuffts University


JoVE 2960

Date Published: September 26, 2011  |  doi:10.3791/2960  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

RNA Isolation of Pseudomonas aeruginosa Colonizing the Murine Gastrointestinal Tract

1Department of Pediatrics, University of Texas Southwestern Medical Center, 2Channing Laboratory, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 3Department of Pediatrics and Microbiology, University of Texas Southwestern Medical Center


JoVE 3293

Date Published: September 28, 2011  |  doi:10.3791/3293  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Separation of Single-stranded DNA, Double-stranded DNA and RNA from an Environmental Viral Community Using Hydroxyapatite Chromatography

1Department of Microbial and Environmental Genomics, The J. Craig Venter Institute, 2Department of Synthetic Biology and Bioenergy, The J. Craig Venter Institute


JoVE 3146

Date Published: September 29, 2011  |  doi:10.3791/3146  |  Section: JoVE Immunology and Infection

 JoVE Clinical and Translational Medicine

Guide Wire Assisted Catheterization and Colored Dye Injection for Vascular Mapping of Monochorionic Twin Placentas

1Division of Pediatric and Fetal Surgery, Department of Surgery, University of California, San Francisco, 2Department of Pathology, University of Alberta, 3Department of Obstretics and Gynecology, University of California, San Francisco, 4Department of Radiology, University of California, San Francisco


JoVE 2837

Date Published: September 5, 2011  |  doi:10.3791/2837  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Accurate and Simple Evaluation of Vascular Anastomoses in Monochorionic Placenta using Colored Dye

1Division of Neonatology, Department of Pediatrics, Leiden University Medical Center, 2Division of Fetal Therapy, Department of Obstetrics, Leiden University Medical Center, 3Department of Obstetrics, Leiden University Medical Center


JoVE 3208

Date Published: September 5, 2011  |  doi:10.3791/3208  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Probing the Brain in Autism Using fMRI and Diffusion Tensor Imaging

1Department of Psychology, University of Alabama at Birmingham


JoVE 3178

Date Published: September 12, 2011  |  doi:10.3791/3178  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Changing the Direction and Orientation of Electric Field During Electric Pulses Application Improves Plasmid Gene Transfer in vitro

1Department of Fundamentals of Electrical Engineering, Mathematics and Physics, University of Ljubljana, 2Department of Biomedical Engineering, University of Ljubljana


JoVE 3309

Date Published: September 12, 2011  |  doi:10.3791/3309  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Intraspinal Cell Transplantation for Targeting Cervical Ventral Horn in Amyotrophic Lateral Sclerosis and Traumatic Spinal Cord Injury

1Department of Neuroscience, Thomas Jefferson University Medical College


JoVE 3069

Date Published: September 18, 2011  |  doi:10.3791/3069  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics

1Merck Research Laboratory, Merck & Co., Inc


JoVE 3010

Date Published: September 22, 2011  |  doi:10.3791/3010  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Mesenteric Artery Contraction and Relaxation Studies Using Automated Wire Myography

1Julius L. Chambers Biomedical/Biotechnology Research Institute, North Carolina Central University, Durham, 2Department of Biology, North Carolina Central University, Durham, 3Department of Physiology & Pharmacology and Hypertension & Vascular Research Center, Wake Forest University School of Medicine


JoVE 3119

Date Published: September 22, 2011  |  doi:10.3791/3119  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

A Simple Guide Screw Method for Intracranial Xenograft Studies in Mice

1Monash Institute of Medical Research, 2MD Anderson Cancer Centre, University of Texas


JoVE 3157

Date Published: September 26, 2011  |  doi:10.3791/3157  |  Section: JoVE Clinical and Translational Medicine

 JoVE Bioengineering

Autologous Endothelial Progenitor Cell-Seeding Technology and Biocompatibility Testing For Cardiovascular Devices in Large Animal Model

1Department of Biomedical Engineering, Duke University, 2School of Medicine, Duke University, 3Department of Surgery, Duke University Medical Center, 4School of Medicine, University of Pennsylvania


JoVE 3197

Date Published: September 9, 2011  |  doi:10.3791/3197  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Formulation of Diblock Polymeric Nanoparticles through Nanoprecipitation Technique

1Laboratory of Nano- and Translational Medicine, Department of Radiation Oncology, Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, 2Carolina Center for Nanotechnology Excellence, University of North Carolina


JoVE 3398

Date Published: September 20, 2011  |  doi:10.3791/3398  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Utilization of Plasmonic and Photonic Crystal Nanostructures for Enhanced Micro- and Nanoparticle Manipulation

1Electrical Engineering Department, University of Washington, 2Division of Human Biology, Fred Hutchinson Cancer Research Center, 3Molecular and Cellular Biology Program, University of Washington, 4Clinical Research, Fred Hutchinson Cancer Research Center, 5Public Health Sciences, Fred Hutchinson Cancer Research Center


JoVE 3390

Date Published: September 27, 2011  |  doi:10.3791/3390  |  Section: JoVE Bioengineering

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