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Table of Contents

Table of Contents: Issue 73, March 2013

Jump to:  JoVE Editorial (1) JoVE Biology (12) JoVE Neuroscience (9) JoVE Immunology and Infection (7) JoVE Clinical and Translational Medicine (14) JoVE Bioengineering (6) JoVE Applied Physics (2) JoVE Chemistry (2)
 JoVE Biology

Rapid and Efficient Generation of Neurons from Human Pluripotent Stem Cells in a Multititre Plate Format

1Max Planck Institute for Molecular Biomedicine, 2Medical Faculty, University of Münster


JoVE 4335

Date Published: March 5, 2013  |  doi:10.3791/4335  |  Section: JoVE Biology

 JoVE Biology

Generation of Stable Human Cell Lines with Tetracycline-inducible (Tet-on) shRNA or cDNA Expression

1UCL Cancer Institute, 2Friedrich Miescher Institute for Biomedical Research


JoVE 50171

Date Published: March 5, 2013  |  doi:10.3791/50171  |  Section: JoVE Biology

 JoVE Biology

Linking Predation Risk, Herbivore Physiological Stress and Microbial Decomposition of Plant Litter

1School of Forestry and Environmental Studies, Yale University, 2Department of Biological Sciences, Virginia Tech, 3Department of Ecology, Evolution and Behavior, The Hebrew University of Jerusalem


JoVE 50061

Date Published: March 12, 2013  |  doi:10.3791/50061  |  Section: JoVE Biology

 JoVE Biology

Isolation and Kv Channel Recordings in Murine Atrial and Ventricular Cardiomyocytes

1Experimental and Clinical Research Center (ECRC), Charité Medical Faculty and Max-Delbrück Center for Molecular Medicine (MDC), 2Medical Department, Division of Cardiology, Campus Virchow-Klinikum, Charité - Universitätsmedizin Berlin, 3Medical Department, Division of Cardiology and Angiology, Campus Mitte, Charité - Universitätsmedizin Berlin


JoVE 50145

Date Published: March 12, 2013  |  doi:10.3791/50145  |  Section: JoVE Biology

 JoVE Biology

Single-molecule Imaging of Gene Regulation In vivo Using Cotranslational Activation by Cleavage (CoTrAC)

1Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, 2Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 3Department of Physics, Jilin University


JoVE 50042

Date Published: March 15, 2013  |  doi:10.3791/50042  |  Section: JoVE Biology

 JoVE Biology

Purification of Transcripts and Metabolites from Drosophila Heads

1Department of Neurology, McKnight Brain Institute, University of Florida, 2Department of Entomology and Nematology, University of Florida, 3Genetics Institute, Department of Molecular Genetics and Microbiology, University of Florida, 4McKnight Brain Institute, Department of Neuroscience, Genetics Institute, Center for Translational Research on Neurodegenerative Diseases, and Center for Movement Disorders and Neurorestoration, University of Florida


JoVE 50245

Date Published: March 15, 2013  |  doi:10.3791/50245  |  Section: JoVE Biology

 JoVE Biology

Isolation and Culture of Individual Myofibers and their Satellite Cells from Adult Skeletal Muscle

1Sprott Center for Stem Cell Research, Ottawa Hospital Research Institute, 2Department of Cellular and Molecular Medicine, University of Ottawa


JoVE 50074

Date Published: March 22, 2013  |  doi:10.3791/50074  |  Section: JoVE Biology

 JoVE Biology

3D Printing of Preclinical X-ray Computed Tomographic Data Sets

1Department of Chemistry and Biochemistry, University of Notre Dame, 2Freimann Life Science Center, University of Notre Dame, 3Department of Biological Sciences, University of Notre Dame, 4Notre Dame Integrated Imaging Facility, University of Notre Dame, 5MakerBot Industries LLC, 6Departments of Biological Sciences, Aerospace and Mechanical Engineering, and Anthropology, University of Notre Dame, 7Harper Cancer Research Institute, University of Notre Dame


JoVE 50250

Date Published: March 22, 2013  |  doi:10.3791/50250  |  Section: JoVE Biology

 JoVE Biology

Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells

1UCSF Diabetes Center and Department of Cell and Tissue Biology, University of California, San Francisco, 2Department of Biology, University of Copenhagen, Denmark, 3National Institute of Nutrition and Seafood Research, Bergen, Norway


JoVE 50191

Date Published: March 28, 2013  |  doi:10.3791/50191  |  Section: JoVE Biology

 JoVE Biology

Live Imaging of GFP-labeled Proteins in Drosophila Oocytes

1Department of Biology, Vassar College


JoVE 50044

Date Published: March 29, 2013  |  doi:10.3791/50044  |  Section: JoVE Biology

 JoVE Biology

Biochemical and High Throughput Microscopic Assessment of Fat Mass in Caenorhabditis Elegans

1Center for Human Genetic Research and Department of Medicine, Massachusetts General Hospital and Harvard Medical School, 2Department of Earth, Atmospheric, and Planetary Sciences, Massachusetts Institute of Technology


JoVE 50180

Date Published: March 30, 2013  |  doi:10.3791/50180  |  Section: JoVE Biology

 JoVE Biology

Analysis of Gene Function and Visualization of Cilia-Generated Fluid Flow in Kupffer's Vesicle

1Department of Cell and Developmental Biology, State University of New York, Upstate Medical University, 2Department of Neurobiology and Anatomy, Eccles Institute of Human Genetics, University of Utah


JoVE 50038

Date Published: March 31, 2013  |  doi:10.3791/50038  |  Section: JoVE Biology

 JoVE Neuroscience

Labeling of Single Cells in the Central Nervous System of Drosophila melanogaster

1Institute of Genetics, University of Mainz, 2Department of Anatomy and Neuroscience, University of Melbourne


JoVE 50150

Date Published: March 4, 2013  |  doi:10.3791/50150  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Mitochondria-associated ER Membranes (MAMs) and Glycosphingolipid Enriched Microdomains (GEMs): Isolation from Mouse Brain

1Department of Genetics, St Jude Children's Research Hospital


JoVE 50215

Date Published: March 4, 2013  |  doi:10.3791/50215  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Simultaneous Pre- and Post-synaptic Electrophysiological Recording from Xenopus Nerve-muscle Co-cultures

1Department of Physiology, David Geffen School of Medicine at UCLA, 2Natural Science Division, Pepperdine University


JoVE 50253

Date Published: March 11, 2013  |  doi:10.3791/50253  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Isolation of Cerebrospinal Fluid from Rodent Embryos for use with Dissected Cerebral Cortical Explants

1Department of Physical Medicine and Rehabilitation, VA Greater Los Angeles Healthcare System, 2Department of Pharmacology and Physiology, Institute for Neuroscience, The George Washington University School of Medicine and Health Sciences, 3Division of Genetics, Department of Medicine, Boston Children's Hospital, 4Howard Hughes Medical Institute, Boston Children's Hospital, 5Department of Pathology, Boston Children's Hospital, Harvard Medical School


JoVE 50333

Date Published: March 11, 2013  |  doi:10.3791/50333  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Selective Tracing of Auditory Fibers in the Avian Embryonic Vestibulocochlear Nerve

1Department of Neurobiology and Behavior, University of California, Irvine


JoVE 50305

Date Published: March 18, 2013  |  doi:10.3791/50305  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Stereotaxic Injection of a Viral Vector for Conditional Gene Manipulation in the Mouse Spinal Cord

1Département Nociception et Douleur, Institut des Neurosciences Cellulaires et Intégratives, Centre National de la Recherche Scientifique (CNRS), 2Departments of Anesthesiology and Pharmacology, Columbia University, 3Department of Anesthesiology, Niigata University Graduate School of Medical and Dental Sciences


JoVE 50313

Date Published: March 18, 2013  |  doi:10.3791/50313  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Extracellularly Identifying Motor Neurons for a Muscle Motor Pool in Aplysia californica

1Department of Biology, Case Western Reserve University, 2Department of Neurosciences, Case Western Reserve University, 3Department of Biomedical Engineering, Case Western Reserve University


JoVE 50189

Date Published: March 25, 2013  |  doi:10.3791/50189  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Chicken Embryo Spinal Cord Slice Culture Protocol

1Research Department of Cell and Developmental Biology, University College London


JoVE 50295

Date Published: March 25, 2013  |  doi:10.3791/50295  |  Section: JoVE Neuroscience

 JoVE Neuroscience

An Injury Paradigm to Investigate Central Nervous System Repair in Drosophila

1Neurodevelopment Group, School of Biosciences, University of Birmingham


JoVE 50306

Date Published: March 28, 2013  |  doi:10.3791/50306  |  Section: JoVE Neuroscience

 JoVE Immunology and Infection

Separation of Plasmodium falciparum Late Stage-infected Erythrocytes by Magnetic Means

1Centro de Biología Celular y Molecular de Enfermedades, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP), 2Acharya Nagarjuna University, 3Departamento de Medios y Creativo, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP)


JoVE 50342

Date Published: March 2, 2013  |  doi:10.3791/50342  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Measurement of Antibody Effects on Cellular Function of Isolated Cardiomyocytes

1Department of Internal Medicine B, University Medicine Greifswald


JoVE 4237

Date Published: March 8, 2013  |  doi:10.3791/4237  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse

1Center for Molecular Imaging (CMI), University of Texas Health Science Center-Houston


JoVE 4326

Date Published: March 8, 2013  |  doi:10.3791/4326  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Ex Vivo Red Blood Cell Hemolysis Assay for the Evaluation of pH-responsive Endosomolytic Agents for Cytosolic Delivery of Biomacromolecular Drugs

1Department of Biomedical Engineering, Vanderbilt University, 2Vanderbilt Institute for Nanoscale Science & Engineering, Vanderbilt University, 3Interdisciplinary Materials Science Program, Vanderbilt University, 4Monroe Carell Jr. Children's Hospital, Vanderbilt University Medical Center, 5Department of Chemical & Biomolecular Engineering, Vanderbilt University, 6Department of Cancer Biology, Vanderbilt University


JoVE 50166

Date Published: March 9, 2013  |  doi:10.3791/50166  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Isolating And Immunostaining Lymphocytes and Dendritic Cells from Murine Peyer's Patches

1Division of Infectious Diseases, New York State Department of Health


JoVE 50167

Date Published: March 17, 2013  |  doi:10.3791/50167  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

A Visual Assay to Monitor T6SS-mediated Bacterial Competition

1MRC Centre for Molecular Bacteriology and Infection, Division of Cell and Molecular Biology, Imperial College London


JoVE 50103

Date Published: March 20, 2013  |  doi:10.3791/50103  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Monitoring Dendritic Cell Migration using 19F / 1H Magnetic Resonance Imaging

1Experimental and Clinical Research Center, A joint cooperation between the Charité Medical Faculty and the Max Delbrück Center for Molecular Medicine, 2Berlin Ultrahigh Field Facility (B.U.F.F.), Max Delbrück Center for Molecular Medicine


JoVE 50251

Date Published: March 20, 2013  |  doi:10.3791/50251  |  Section: JoVE Immunology and Infection

 JoVE Clinical and Translational Medicine

Murine Spinotrapezius Model to Assess the Impact of Arteriolar Ligation on Microvascular Function and Remodeling

1Department of Biomedical Engineering, University of Virginia, 2Department of Biomedical Engineering, California Polytechnic State University, 3Office of Animal Welfare, University of Virginia, 4Department of Biomedical Engineering & Institute for Computational Medicine, Johns Hopkins University


JoVE 50218

Date Published: March 3, 2013  |  doi:10.3791/50218  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Surgical Procedures for a Rat Model of Partial Orthotopic Liver Transplantation with Hepatic Arterial Reconstruction

1Institute for Laboratory Animal Science and Experimental Surgery, RWTH-Aachen University, 2Department of Hepato-Biliary-Pancreatic Surgery and Transplantation, Graduate School of Medicine, Kyoto University


JoVE 4376

Date Published: March 7, 2013  |  doi:10.3791/4376  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Using Quantitative Real-time PCR to Determine Donor Cell Engraftment in a Competitive Murine Bone Marrow Transplantation Model

1Division of Hematology-oncology, Department of Medicine, Medical University of South Carolina


JoVE 50193

Date Published: March 7, 2013  |  doi:10.3791/50193  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Transplantation into the Anterior Chamber of the Eye for Longitudinal, Non-invasive In vivo Imaging with Single-cell Resolution in Real-time

1Diabetes Research Institute, University of Miami Miller School of Medicine, 2Department of Surgery, University of Miami Miller School of Medicine, 3Department of Medicine, University of Miami Miller School of Medicine, 4Department of Physiology & Biophysics, University of Miami Miller School of Medicine, 5The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet


JoVE 50466

Date Published: March 10, 2013  |  doi:10.3791/50466  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma

1Institute of Pathology, Laboratory of Molecular Tumor Pathology, Charité - Universitätsmedizin Berlin, 2Institute for Chemistry and Biochemistry, Free University Berlin, 3Laboratory for Functional Genomics Charité (LFGC), Charité - Universitätsmedizin Berlin, 4Comprehensive Cancer Center Charité, Charité - Universitätsmedizin Berlin


JoVE 50200

Date Published: March 13, 2013  |  doi:10.3791/50200  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Non-invasive Optical Measurement of Cerebral Metabolism and Hemodynamics in Infants

1Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School, 2Lab. PALM, Université de Caen Basse-Normandie, 3Fetal-Neonatal Neuroimaging and Developmental Science Center, Boston Children's Hospital, Harvard Medical School, 4ISS, INC.


JoVE 4379

Date Published: March 14, 2013  |  doi:10.3791/4379  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

An in vivo Assay to Test Blood Vessel Permeability

1Fox Chase Cancer Center


JoVE 50062

Date Published: March 16, 2013  |  doi:10.3791/50062  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Tilt Testing with Combined Lower Body Negative Pressure: a "Gold Standard" for Measuring Orthostatic Tolerance

1Department of Biomedical Physiology and Kinesiology, Simon Fraser University


JoVE 4315

Date Published: March 21, 2013  |  doi:10.3791/4315  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

A Simple Method of Mouse Lung Intubation

1Department of Environmental Health Sciences, Program in Respiratory Biology and Lung Disease, Johns Hopkins Bloomberg School of Public Health, 2Department of Pediatrics, Oregon Health Sciences University


JoVE 50318

Date Published: March 21, 2013  |  doi:10.3791/50318  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Assessment of Gastric Emptying in Non-obese Diabetic Mice Using a [13C]-octanoic Acid Breath Test

1Enteric Neuroscience Program, Department of Physiology and Biomedical Engineering, Mayo Clinic


JoVE 50301

Date Published: March 23, 2013  |  doi:10.3791/50301  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Live Imaging of Drug Responses in the Tumor Microenvironment in Mouse Models of Breast Cancer

1Watson School of Biological Sciences, 2Cold Spring Harbor Laboratory, 3Departments of Medical Genetics, University of Oslo and Oslo University Hospital


JoVE 50088

Date Published: March 24, 2013  |  doi:10.3791/50088  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Molecular Imaging to Target Transplanted Muscle Progenitor Cells

1Imaging Program, Lawson Health Research Institute, 2Department of Anatomy and Cell Biology, Western University, 3Department of Medical Biophysics, Western University


JoVE 50119

Date Published: March 27, 2013  |  doi:10.3791/50119  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Development of an Audio-based Virtual Gaming Environment to Assist with Navigation Skills in the Blind

1Laboratory for Visual Neuroplasticity, Department of Ophthalmology, Massachusetts Eye and Ear Infirmary, Harvard Medical School, 2Department of Computer Science and Center for Advanced Research in Education (CARE), University of Chile


JoVE 50272

Date Published: March 27, 2013  |  doi:10.3791/50272  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Modified Technique for Coronary Artery Ligation in Mice

1Wallenberg Laboratory, Sahlgrenska Academy, University of Gothenburg


JoVE 3093

Date Published: March 29, 2013  |  doi:10.3791/3093  |  Section: JoVE Clinical and Translational Medicine

 JoVE Bioengineering

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

1Biomedical Engineering Department, Johns Hopkins University School of Medicine, 2Translational Tissue Engineering Center, Johns Hopkins University School of Medicine, 3Wilmer Eye Institute, Johns Hopkins University School of Medicine, 4Institute for Nanobiotechnology, Johns Hopkins University School of Medicine


JoVE 50176

Date Published: March 1, 2013  |  doi:10.3791/50176  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Graphene Coatings for Biomedical Implants

1Department of Physics, Clemson University, 2Department of Pharmacology and Toxicology, East Carolina University, 3Department of Bioengineering, Clemson University, 4Center for Optical Materials Science and Engineering Technologies, Clemson University


JoVE 50276

Date Published: March 1, 2013  |  doi:10.3791/50276  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Engineering Skeletal Muscle Tissues from Murine Myoblast Progenitor Cells and Application of Electrical Stimulation

1Department of Biomedical Engineering, Soft Tissue Biomechanics and Engineering, Eindhoven University of Technology, The Netherlands


JoVE 4267

Date Published: March 19, 2013  |  doi:10.3791/4267  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Generation of Aligned Functional Myocardial Tissue Through Microcontact Printing

1Cardiovascular Research Center, Massachusetts General Hospital and Harvard Medical School, 2Harvard Stem Cell Institute


JoVE 50288

Date Published: March 19, 2013  |  doi:10.3791/50288  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Cell Co-culture Patterning Using Aqueous Two-phase Systems

1Department of Biomedical Engineering, University of Michigan, 2Department of Macromolecular Science and Engineering, University of Michigan


JoVE 50304

Date Published: March 26, 2013  |  doi:10.3791/50304  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Microfluidic Chip Fabrication and Method to Detect Influenza

1Department of Mechanical Engineering, Boston University, 2Department of Biomedical Engineering, Boston University


JoVE 50325

Date Published: March 26, 2013  |  doi:10.3791/50325  |  Section: JoVE Bioengineering

 JoVE Applied Physics

Determining 3D Flow Fields via Multi-camera Light Field Imaging

1Department of Mechanical Engineering, Brigham Young University, 2Naval Undersea Warfare Center, Newport, RI


JoVE 4325

Date Published: March 6, 2013  |  doi:10.3791/4325  |  Section: JoVE Applied Physics

 JoVE Applied Physics

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing

1Department of Physics, University of Alberta


JoVE 50256

Date Published: March 13, 2013  |  doi:10.3791/50256  |  Section: JoVE Applied Physics

 JoVE Chemistry

Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies

1Structural Biology Division, The Walter and Eliza Hall Institute of Medical Research, 2The University of Melbourne


JoVE 50141

Date Published: March 6, 2013  |  doi:10.3791/50141  |  Section: JoVE Chemistry

 JoVE Chemistry

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)

1Proteomics and Metabolomics Facility, Colorado State University


JoVE 50242

Date Published: March 14, 2013  |  doi:10.3791/50242  |  Section: JoVE Chemistry

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