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The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.

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Table of Contents

Table of Contents: Issue 44, October 2010

Jump to:  JoVE General (11) JoVE Neuroscience (8) JoVE Immunology and Infection (10) JoVE Clinical and Translational Medicine (4)
 JoVE General

Live Cell Response to Mechanical Stimulation Studied by Integrated Optical and Atomic Force Microscopy


JoVE 2072

1,2, 1

1Department of Systems Biology and Translational Medicine, College of Medicine, Cardiovascular Research Institute, Texas A&M Health Science Center, 2Department of Biomedical Engineering, Texas A&M University

Date Published: October 4, 2010  |  doi:10.3791/2072  |  Section: JoVE General

Keywords: Cellular Biology, live cells, mechanical stimulation, integrated microscopy, atomic force microscopy, spinning-disk confocal, total internal reflection fluorescence

 JoVE General

Gramicidin-based Fluorescence Assay; for Determining Small Molecules Potential for Modifying Lipid Bilayer Properties


JoVE 2131

, , ,

Department of Physiology and Biophysics, Weill Cornell Medical College

Date Published: October 13, 2010  |  doi:10.3791/2131  |  Section: JoVE General

Keywords: Microbiology, membrane properties, bilayer properties, gramicidin, fluorescence quenching, high throughput drug screening

 JoVE General

Changes in Mammary Gland Morphology and Breast Cancer Risk in Rats


JoVE 2260

1, 1,2, 1, 1

1Department of Oncology, Georgetown University, 2Institute of Biomedicine, University of Turku Medical Faculty

Date Published: October 16, 2010  |  doi:10.3791/2260  |  Section: JoVE General

Keywords: Medicine, mammary gland morphology, terminal end buds, mammary cancer, maternal dietary exposures, pregnancy, prepubertal dietay exposures

 JoVE General

Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering (CARS)


JoVE 2085

1, 1,2

1Center for Biophotonics, University of California, Davis, 2Department of Internal Medicine, University of California, Davis

Date Published: October 17, 2010  |  doi:10.3791/2085  |  Section: JoVE General

Keywords: Cellular Biology, Raman scattering, Four-wave mixing, Coherent anti-Stokes Raman scattering, Microscopy, Coherent Raman Scattering

 JoVE General

An ex-ovo Chicken Embryo Culture System Suitable for Imaging and Microsurgery Applications


JoVE 2154

1,2, 1, 1, 1

1Department of Biomedical Engineering, Cornell University, 2Current Address: Mechanical Engineering Department, Dogus University

Date Published: October 23, 2010  |  doi:10.3791/2154  |  Section: JoVE General

Keywords: Developmental Biology, chicken embryo, ex-ovo culture, developmental biology, microsurgery, imaging, microinjection, ligation

 JoVE General

Assessing Two-dimensional Crystallization Trials of Small Membrane Proteins for Structural Biology Studies by Electron Crystallography


JoVE 1846

*1, *1,2, *3, *1, 3, 1,3

1School of Biology, Georgia Institute of Technology, 2Department of Molecular Pharmacology, RWTH Aachen University, 3School of Chemistry and Biochemistry, Georgia Institute of Technology

Date Published: October 29, 2010  |  doi:10.3791/1846  |  Section: JoVE General

Keywords: Cellular Biology, membrane protein, structure, two-dimensional crystallization, electron crystallography, electron microscopy, screening

 JoVE Neuroscience

Intracranial Orthotopic Allografting of Medulloblastoma Cells in Immunocompromised Mice


JoVE 2153

1, 2, 1, 1, 2, 1

1Department of Cell and Developmental Biology, Vanderbilt University, 2Department of Neurology, Vanderbilt University

Date Published: October 3, 2010  |  doi:10.3791/2153  |  Section: JoVE Neuroscience

Keywords: Neuroscience, medulloblastoma, intracranial, orthotopic, grafting, immunocompromised mice

 JoVE Neuroscience

In utero Electroporation followed by Primary Neuronal Culture for Studying Gene Function in Subset of Cortical Neurons


JoVE 2103

1, 1, 1, 2, 1

1Center for Neurologic Diseases, Brigham and Woman's Hospital and Harvard Medical School, 2Department of Physiology and Neurobiology, University of Connecticut

Date Published: October 8, 2010  |  doi:10.3791/2103  |  Section: JoVE Neuroscience

Keywords: Neuroscience, In utero electroporation, cortical neurons, neurite outgrowth, migration, neuroscience, development, brain

 JoVE Immunology and Infection

Co-culture Models of Pseudomonas aeruginosa Biofilms Grown on Live Human Airway Cells


JoVE 2186

1, 2, 1, 2

1Department of Physiology, Dartmouth College, 2Department of Biology, Indiana University Purdue University Indianapolis

Date Published: October 6, 2010  |  doi:10.3791/2186  |  Section: JoVE Immunology and Infection

Keywords: Cellular Biology, biofilm, Pseudomonas aeruginosa, airway, epithelial cells, co-culture, cytotoxicity, Cystic Fibrosis, virulence

 JoVE Immunology and Infection

Visualizing Cell-to-cell Transfer of HIV using Fluorescent Clones of HIV and Live Confocal Microscopy


JoVE 2061

1, 2, 2, 3, 2, 1

1Division of Infectious Diseases, Department of Medicine, Immunology Institute, Mount Sinai School of Medicine, 2NSF Center for Biophotonics, University of California, Davis, 3Structural and Computational Biology Unit, European Molecular Biology Laboratory

Date Published: October 7, 2010  |  doi:10.3791/2061  |  Section: JoVE Immunology and Infection

Keywords: Cellular Biology, HIV, GFP, live confocal imaging, virological synapse

 JoVE Immunology and Infection

The Use of Carboxyfluorescein Diacetate Succinimidyl Ester (CFSE) to Monitor Lymphocyte Proliferation


JoVE 2259

,

Department of Immunology, John Curtin School of Medical Research, Australian National University

Date Published: October 12, 2010  |  doi:10.3791/2259  |  Section: JoVE Immunology and Infection

Keywords: Immunology, carboxyfluorescein diacetate succinimidyl ester (CFSE), labeling, lymphocytes, proliferation.

 JoVE Immunology and Infection

Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce


JoVE 2308

1, 2

1Center for Meat Safety and Quality, Department of Animal Sciences, Colorado State University, 2Rapid Microbial Detection and Control Laboratory, Department of Food Science and Human Nutrition, Iowa State University

Date Published: October 18, 2010  |  doi:10.3791/2308  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Salmonella, adhesive tape, rapid detection, fresh produce, fluorescence in situ hybridization, fluorescence microscopy, flow cytometry

 JoVE Immunology and Infection

In vitro tRNA Methylation Assay with the Entamoeba histolytica DNA and tRNA Methyltransferase Dnmt2 (Ehmeth) Enzyme


JoVE 2390

1, 2, 2, 1

1Faculty of Medicine, Rappaport Institute, Technion - Israel Institute of Technology, 2The Pharmacy and Biochemistry Institute, Johannes Gutenberg University

Date Published: October 19, 2010  |  doi:10.3791/2390  |  Section: JoVE Immunology and Infection

Keywords: Immunology, tRNA, methylation, DNA methyltransferase 2, Entamoeba histolytica

 JoVE Immunology and Infection

A 96 Well Microtiter Plate-based Method for Monitoring Formation and Antifungal Susceptibility Testing of Candida albicans Biofilms


JoVE 2287

1,2, 1,2, 1,2, 1,2

1Department of Biology, University of Texas San Antonio - UTSA, 2South Texas Center for Emerging Infectious Diseases, University of Texas San Antonio - UTSA

Date Published: October 21, 2010  |  doi:10.3791/2287  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Microbiology, Medical Mycology, Candida, candidiasis, biofilms, antifungals

 JoVE Immunology and Infection

Retroviral Transduction of T-cell Receptors in Mouse T-cells


JoVE 2307

1, 1,2, 1, 1

1NYU Cancer institute, New York University School of Medicine, 2Program in Structural Biology, New York University School of Medicine

Date Published: October 22, 2010  |  doi:10.3791/2307  |  Section: JoVE Immunology and Infection

Keywords: Immunology, T-cell, T-cell receptor, Retrovirus, Mouse, Transduction, Spleen

 JoVE Immunology and Infection

Comprehensive & Cost Effective Laboratory Monitoring of HIV/AIDS: an African Role Model


JoVE 2312

1,2, 2, 3, 1,2

1National Health Laboratory Services (NHLS-SA), 2Department of Molecular Medicine and Haematology, University of Witwatersrand, 3Lightcurve Films

Date Published: October 31, 2010  |  doi:10.3791/2312  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Human Immunodeficiency virus (HIV); CD4 lymphocyte count, white cell count, CD45, panleucogating, lymphocyte activation, CD38, HIV viral load, antiretroviral therapy (ART), internal quality control

 JoVE Clinical and Translational Medicine

Multispectral Real-time Fluorescence Imaging for Intraoperative Detection of the Sentinel Lymph Node in Gynecologic Oncology


JoVE 2225

1, 2, 1, 1, 1, 2, 3, 2, 1

1Department of Surgery, Division of Surgical Oncology, University Medical Center Groningen, 2Helmholtz Zentrum, Technical University Munich, 3Department of Obstetrics and Gynaecology, University Medical Center Groningen

Date Published: October 20, 2010  |  doi:10.3791/2225  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Image-guided surgery, multispectral fluorescence, sentinel lymph node, gynecologic oncology

 JoVE Clinical and Translational Medicine

Performing and Processing FNA of Anterior Fat Pad for Amyloid


JoVE 1747

1,2, 1, 3, 3, 4, 5

1Department of Pathology, Medical College of Wisconsin, 2Current Address: Department of Pathology, Wayne State University School of Medicine Detroit Medical Center, 3Department of Neurology, Medical College of Wisconsin, 4Department of Medicine, Medical College of Wisconsin, 5Division of Neoplastic Diseases and Related Disorders, Medical College of Wisconsin

Date Published: October 30, 2010  |  doi:10.3791/1747  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, AL amyloidosis, Congo Red, abdominal fat pad biopsy, electron microscopy, ultrastructural evaluation

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