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The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.

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Table of Contents

Table of Contents: Issue 45, November 2010

Jump to:  JoVE General (12) JoVE Neuroscience (17) JoVE Immunology and Infection (6) JoVE Clinical and Translational Medicine (3)
 JoVE General

Label-free in situ Imaging of Lignification in Plant Cell Walls


JoVE 2064

1, 1, 2, 3, 2

1Energy Biosciences Institute, University of California, Berkeley, 2Molecular Foundry, Lawrence Berkeley National Laboratory, 3Physical Biosciences Division, Lawrence Berkeley National Laboratory

Date Published: November 1, 2010  |  doi:10.3791/2064  |  Section: JoVE General

Keywords: Plant Biology, Raman microscopy, lignin, poplar wood, Arabidopsis thaliana

 JoVE General

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations


JoVE 2279

, ,

Wellcome Trust Centre for Human Genetics, University of Oxford

Date Published: November 3, 2010  |  doi:10.3791/2279  |  Section: JoVE General

Keywords: Cellular Biology, Gene expression, regulatory variant, haplotype, association study, primer extension, MALDI-TOF mass spectrometry, single nucleotide polymorphism, allele-specific

 JoVE General

Closed System Cell Culture Protocol Using HYPERStack Vessels with Gas Permeable Material Technology


JoVE 2499

*1, *2, 2, *2, *3, 3

1Business Development, Corning Life Science, 2Applications, Corning Life Science, 3Product Development, Corning Life Science

Date Published: November 29, 2010  |  doi:10.3791/2499  |  Section: JoVE General

Keywords: Cellular Biology, cell culture, bioprocess, adherent, primary cell, HYPERStack, closed system, gas permeable, cell therapy, vaccine, scale up

 JoVE Neuroscience

How to Create and Use Binocular Rivalry


JoVE 2030

1,2, 3,4, 3,4, 3,4

1Department of Psychology, New York University, 2Centre for Neural Science, New York University, 3Department of Psychology, Princeton University, 4Neuroscience Institute, Princeton University

Date Published: November 10, 2010  |  doi:10.3791/2030  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Binocular rivalry, continuous flash suppression, vision, visual awareness, perceptual competition, unconscious processing, neuroimaging

 JoVE Neuroscience

Cryopreservation of Cortical Tissue Blocks for the Generation of Highly Enriched Neuronal Cultures


JoVE 2384

, , , ,

Department of Neurobiology and Behavior, University of California, Irvine

Date Published: November 11, 2010  |  doi:10.3791/2384  |  Section: JoVE Neuroscience

Keywords: Neuroscience, cryopreservation, neuron, astrocyte, neuronal stem cells, tissue culture, brain cortex

 JoVE Neuroscience

The NeuroStar TMS Device: Conducting the FDA Approved Protocol for Treatment of Depression


JoVE 2345

1, 2, 2, 1

1Berenson-Allen Center for Noninvasive Brain Stimulation, Beth Israel Deaconess Medical Center, 2Neuronetics, Inc.

Date Published: November 12, 2010  |  doi:10.3791/2345  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Transcranial Magnetic Stimulation, Depression, Neuronetics, NeuroStar, FDA Approved

 JoVE Neuroscience

Ultrasound-Guided Microinjection into the Mouse Forebrain In Utero at E9.5


JoVE 2047

1, 1,2

1Institute for Cell Engineering Neuroregeneration and Stem Cell Programs, Johns Hopkins University School of Medicine, 2Departments of Neurology, Neuroscience, and Oncology, Johns Hopkins University School of Medicine

Date Published: November 13, 2010  |  doi:10.3791/2047  |  Section: JoVE Neuroscience

Keywords: Neuroscience, high-frequency ultrasound imaging, rodent survival surgery, intra-ventricular microinjection, retroviral vectors, gene transduction

 JoVE Neuroscience

Automated Sholl Analysis of Digitized Neuronal Morphology at Multiple Scales


JoVE 2354

*1,2, *1,2, 1, 1, 1

1Department of Cell Biology and Neuroscience, Rutgers University, 2Graduate Program in Biomedical Engineering, Rutgers University

Date Published: November 14, 2010  |  doi:10.3791/2354  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Sholl Analysis, Neurite, Morphology, Computer-assisted, Tracing

 JoVE Neuroscience

Visualization of Mitochondrial DNA Replication in Individual Cells by EdU Signal Amplification


JoVE 2147

1, 2, 3

1Michigan Research Community, Undergraduate Research Opportunity Program, University of Michigan, 2Department of Neurology, University of Michigan, 3Department of Internal Medicine, Division of Metabolism, Endocrinology, and Diabetes, University of Michigan

Date Published: November 15, 2010  |  doi:10.3791/2147  |  Section: JoVE Neuroscience

Keywords: Neuroscience, mitochondria, mitochondrial DNA (mtDNA), 5-ethynyl-2'-deoxyuridine (EdU), labeling, tyramide signal amplification, mtDNA biogenesis, dorsal root ganglion neurons

 JoVE Neuroscience

Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay


JoVE 2393

1,2, 2, 2, 2

1 Department of Anatomical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran, 2Department of Neurosurgery, University of Florida

Date Published: November 20, 2010  |  doi:10.3791/2393  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Adult Neural Stem Cells, Neurosphere Assay, Isolation, Expansion

 JoVE Neuroscience

Production of Chick Embryo Extract for the Cultivation of Murine Neural Crest Stem Cells


JoVE 2380

*1, *1, 2, 2, 1, 1, 1

1Department of Pediatric Oncology, University Children's Hospital Essen, 2Department of Developmental Pathology, Bonn Medical School, Institute of Pathology

Date Published: November 27, 2010  |  doi:10.3791/2380  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Cellular Biology, mice, neural crest, stem cell, chick embryo extract

 JoVE Immunology and Infection

Monitoring Immune Cells Trafficking Fluorescent Prion Rods Hours after Intraperitoneal Infection


JoVE 2349

*, *, , , , ,

Department of Microbiology, Immunology and Pathology, Colorado State University

Date Published: November 19, 2010  |  doi:10.3791/2349  |  Section: JoVE Immunology and Infection

Keywords: Immunology, prions, mouse, trafficking, intraperitoneal, lymph nodes, flow cytometry

 JoVE Immunology and Infection

Enzyme-linked Immunospot Assay (ELISPOT): Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens


JoVE 2221

*1, *1, 2, 1,2

1Department of Medicine, Vanderbilt University School of Medicine, 2Department of Microbiology and Immunology, Vanderbilt University School of Medicine

Date Published: November 23, 2010  |  doi:10.3791/2221  |  Section: JoVE Immunology and Infection

Keywords: Immunology, ELISPOT, Th-1 Immune Response, interferon gamma, T cell, adaptive immunity

 JoVE Immunology and Infection

An Orthotopic Model of Serous Ovarian Cancer in Immunocompetent Mice for in vivo Tumor Imaging and Monitoring of Tumor Immune Responses


JoVE 2146

1, 2, 1

1Penn Ovarian Cancer Research Center, Center for Research on Reproduction and Womans Health, Department of Obstetrics and Gynecology, University of Pennsylvania-School of Medicine, 2Women's Cancer Program, Fox Chase Cancer Center

Date Published: November 28, 2010  |  doi:10.3791/2146  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Ovarian cancer, syngeneic, orthotopic, katushka (TurboFP635), in vivo imaging, immunocompetent mouse model of ovarian cancer, deep tumors

 JoVE Clinical and Translational Medicine

The C-seal: A Biofragmentable Drain Protecting the Stapled Colorectal Anastomosis from Leakage


JoVE 2223

, , ,

Department of Surgery, Division of Abdominal Surgery, University Medical Center Groningen

Date Published: November 4, 2010  |  doi:10.3791/2223  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Surgery, low anterior resection, colorectal anastomosis, anastomotic leakage, drain, rectal cancer, circular stapler

 JoVE Clinical and Translational Medicine

Bronchial Thermoplasty: A Novel Therapeutic Approach to Severe Asthma


JoVE 2428

1, 2

1Director of Pulmonary Special Procedures and the Lung Cancer Center, Virginia Hospital Center, 2Chief, Division of Pulmonary Critical Care and Sleep Medicine, Virginia Hospital Center

Date Published: November 4, 2010  |  doi:10.3791/2428  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, bronchial thermoplasty, severe asthma, airway smooth muscle, bronchoscopy, radiofrequency energy, patient management, moderate sedation

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