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The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.

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Table of Contents

Table of Contents: Issue 51, May 2011

Jump to:  JoVE Editorial (1) JoVE General (15) JoVE Neuroscience (14) JoVE Immunology and Infection (10) JoVE Clinical and Translational Medicine (11) JoVE Bioengineering (4) JoVE Errata (5)
 JoVE General

Ex Vivo Culture of Primary Human Fallopian Tube Epithelial Cells


JoVE 2728

1,2, 1,2,3, 1,2,4

1Department of Medical Oncology, Dana-Farber Cancer Institute, 2Harvard Medical School, Boston, MA, 3Sheba Cancer Research Center, Chaim Sheba Medical Center, 4Department of Pathology, Brigham and Women's Hospital

Date Published: May 9, 2011  |  doi:10.3791/2728  |  Section: JoVE General

Keywords: Cellular Biology, Primary human epithelial cells, ovarian cancer, serous, ex-vivo, cell biology, fallopian tube, fimbria

 JoVE General

Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo


JoVE 2620

,

Laboratory of Stem Cell and Neuro-Vascular Biology, Genetics and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health

Date Published: May 20, 2011  |  doi:10.3791/2620  |  Section: JoVE General

Keywords: Developmental Biology, Confocal microscopy, whole-mount immunohistochemistry, mouse embryo, blood vessel, lymphatic vessel, vascular patterning, arterial differentiation

 JoVE Neuroscience

Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence


JoVE 1694

1, 2, 1, 3, 1, 3, 1, 1, 1,4, 3, 2, 1

1Optics Division, Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, 2Department of Biochemistry and Biophysics, University of Pennsylvania, 3Neuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital and Harvard Medical School, 4Departments of Neurosciences and Radiology, University of California

Date Published: May 4, 2011  |  doi:10.3791/1694  |  Section: JoVE Neuroscience

Keywords: Neuroscience, brain, blood, oxygenation, phosphorescence, imaging

 JoVE Neuroscience

Brain Imaging Investigation of the Memory-Enhancing Effect of Emotion


JoVE 2433

1, 2, 3, 1,4

1Centre for Neuroscience, University of Alberta, 2Neuroscience Program, University of Illinois, Urbana-Champaign, 3Center for Cognitive Neuroscience, Duke University, 4Psychology Department, Neuroscience Program, & Beckman Institute, University of Illinois, Urbana-Champaign

Date Published: May 4, 2011  |  doi:10.3791/2433  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Affect, Recognition, Recollection, Dm Effect, Neuroimaging

 JoVE Neuroscience

Methods for Experimental Manipulations after Optic Nerve Transection in the Mammalian CNS


JoVE 2261

*, *,

Department of Surgery, University of Toronto

Date Published: May 12, 2011  |  doi:10.3791/2261  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Central Nervous System, Retinal Ganglion Cell, Axotomy, Optic Nerve Transection, Intraocular Injection, Nerve Stump Transfection, Viral Vector, Short Interfering RNA

 JoVE Neuroscience

Organotypic Cerebellar Cultures: Apoptotic Challenges and Detection


JoVE 2564

1, 2, 2, 1

1Laboratory of Genetics, The Salk Institute for Biological Studies, 2Clayton Foundation Laboratories for Peptide Biology, The Salk Institute for Biological Studies

Date Published: May 17, 2011  |  doi:10.3791/2564  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Cerebellum, Organotypic, Fas, Apoptosis, Purkinje cell

 JoVE Neuroscience

Electrode Positioning and Montage in Transcranial Direct Current Stimulation


JoVE 2744

1, 2,3, 4, 2

1Headache & Orofacial Pain Effort (H.O.P.E.), Biologic & Material Sciences, School of Dentistry, University of Michigan, 2Laboratory of Neuromodulation, Department of Physical Medicine & Rehabilitation, Spaulding Rehabilitation Hospital and Massachusetts General Hospital, Harvard Medical School, 3Charité, University Medicine Berlin, 4Department of Biomedical Engineering, The City College of New York

Date Published: May 23, 2011  |  doi:10.3791/2744  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Transcranial direct current stimulation, pain, chronic pain, noninvasive brain stimulation, neuromodulation

 JoVE Neuroscience

Coherence between Brain Cortical Function and Neurocognitive Performance during Changed Gravity Conditions


JoVE 2670

1, 1, 1, 1, 2, 3, 4

1Institute of Movement and Neurosciences, German Sport University Cologne, 2Deptartment of Surgical Skills, University of Toronto, 3School of Human Movement Studies, Institute of Health and Biomedical Innovation, Queensland University of Technology, 4Brain Products GmbH, Scientific Support, Gilching, Germany

Date Published: May 23, 2011  |  doi:10.3791/2670  |  Section: JoVE Neuroscience

Keywords: Neuroscience, EEG, NIRS, electrotomography, parabolic flight, weightlessness, imaging, cognitive performance

 JoVE Neuroscience

Generation of Neural Stem Cells from Discarded Human Fetal Cortical Tissue


JoVE 2681

1, 2, 3, 4, 1

1Department of Neurology, Beth Israel Deaconess Medical Center, 2Department of Obstetrics and Gynecology, Brigham and Women's Hospital, 3Department of Pathology, Beth Israel Deaconess Medical Center, 4Department of Pathology, Division of Neuropathology, Brigham and Women's Hospital

Date Published: May 25, 2011  |  doi:10.3791/2681  |  Section: JoVE Neuroscience

Keywords: Neuroscience, neural stem cell, human, brain, cortex, fetus, cell culture

 JoVE Neuroscience

Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals


JoVE 2752

1,2, 1, 3, 1

1Section on Synaptic Pharmacology/Laboratory for Integrative Neuroscience, National Institutes of Health/National Institute on Alcohol Abuse and Alcoholism, 2Department of Electronics Engineering, Ewha Womans University, 3Section on Transmitter Signaling/Laboratory of Molecular Physiology, National Institutes of Health/National Institute on Alcohol Abuse and Alcoholism

Date Published: May 25, 2011  |  doi:10.3791/2752  |  Section: JoVE Neuroscience

Keywords: Neuroscience, neuronal dissociation, synaptic transmission, GABA, calcium imaging, electrophysiology, hippocampus, striatum

 JoVE Immunology and Infection

IgY Technology: Extraction of Chicken Antibodies from Egg Yolk by Polyethylene Glycol (PEG) Precipitation


JoVE 3084

1, 2, 3, 4, 5

1Center for Biological Security, Robert Koch-Institute, 2CICVyA - INTA Castelar, Instituto de Virología, 3Center of Molecular Immunology, Ciudad de la Habana, Cuba, 4Department of Biology, Chemistry, Pharmacy, Institute of Biology-Neurobiology, Free University of Berlin, 5Institut of Pharmacology, Charité-University Medicine of Berlin

Date Published: May 1, 2011  |  doi:10.3791/3084  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Immunization, Chicken, Antibodies, Polyethylene Glycol

 JoVE Immunology and Infection

Non-surgical Intratracheal Instillation of Mice with Analysis of Lungs and Lung Draining Lymph Nodes by Flow Cytometry


JoVE 2702

1, 2, 1, 3, 1,2,4, 1,4

1Department of Immunology, University of Colorado School of Medicine, 2Division of Cell Biology, Department of Pediatrics, National Jewish Health, 3Department of Microbiology, Immunology, and Pathology, Colorado State University, 4Department of Immunology, National Jewish Health

Date Published: May 2, 2011  |  doi:10.3791/2702  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Intratracheal, mouse, lungs, lung draining lymph nodes, flow cytometry

 JoVE Immunology and Infection

Use of Fluorescent Immuno-Chemistry for the detection of Edwardsiella ictaluri in channel catfish (I. punctatus) samples


JoVE 2687

,

Department of Basic Sciences, Mississippi State University

Date Published: May 10, 2011  |  doi:10.3791/2687  |  Section: JoVE Immunology and Infection

Keywords: Immunology, immunohistochemistry, histology, Edwardsiella ictaluri, enteric septicemia of channel catfish, Fish, Catfish, Ictalurus punctatus

 JoVE Immunology and Infection

In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells


JoVE 2783

, , , ,

Universite de Montreal, Groupe de Recherche sur les Maladies Infectieuses du Porc GREMIP, Faculte de medecine veterinaire

Date Published: May 16, 2011  |  doi:10.3791/2783  |  Section: JoVE Immunology and Infection

Keywords: Infection, adhesion, invasion, bacteria, cell culture, microbiology, cell biology

 JoVE Immunology and Infection

Trichuris muris Infection: A Model of Type 2 Immunity and Inflammation in the Gut


JoVE 2774

*1, *1, 1, 1,2

1The Biomedical Research Centre, University of British Columbia, 2Department of Pathology and Laboratory Medicine, University of British Columbia

Date Published: May 24, 2011  |  doi:10.3791/2774  |  Section: JoVE Immunology and Infection

Keywords: Infection, Trichuris muris, mouse, Th2, intestine, inflammation

 JoVE Immunology and Infection

A Simple and Efficient Method to Isolate Macrophages from Mixed Primary Cultures of Adult Liver Cells


JoVE 2757

1, 1, 1, 2, 2

1Transgenic Animal Research Center, National Institute of Agrobiological Sciences, Tsukuba, Japan, 2Safety Research Team, National Institute of Animal Health, Tsukuba, Japan

Date Published: May 24, 2011  |  doi:10.3791/2757  |  Section: JoVE Immunology and Infection

Keywords: Infection, macrophage-like cells, proliferation, hepatocytes, mixed culture, shaking, attachment

 JoVE Immunology and Infection

Generation of Multivirus-specific T Cells to Prevent/treat Viral Infections after Allogeneic Hematopoietic Stem Cell Transplant


JoVE 2736

, , ,

Center for Cell and Gene Therapy, Baylor College of Medicine

Date Published: May 27, 2011  |  doi:10.3791/2736  |  Section: JoVE Immunology and Infection

Keywords: Immunology, T cells, immunotherapy, viral infections, nucleofection, plasmids, G-Rex culture device

 JoVE Clinical and Translational Medicine

Quantitative Visualization and Detection of Skin Cancer Using Dynamic Thermal Imaging


JoVE 2679

,

Department of Mechanical Engineering, The Johns Hopkins University

Date Published: May 5, 2011  |  doi:10.3791/2679  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Infrared imaging, quantitative thermal analysis, image processing, skin cancer, melanoma, transient thermal response, skin thermal models, skin phantom experiment, patient study

 JoVE Clinical and Translational Medicine

Intraluminal Middle Cerebral Artery Occlusion (MCAO) Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice


JoVE 2879

*1, *1,2, 1, 1, 1

1Department of Neurosurgery, University of Florida, 2Department of Anatomical Sciences, Shiraz University of Medical Sciences

Date Published: May 8, 2011  |  doi:10.3791/2879  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Cerebral ischemia, mouse, middle cerebral artery occlusion, intraluminal suture, Laser Doppler

 JoVE Clinical and Translational Medicine

An in vivo Rodent Model of Contraction-induced Injury and Non-invasive Monitoring of Recovery


JoVE 2782

1,2, 1, 2, 2, 3

1Department of Physiology, University of Maryland School of Medicine, 2Department of Orthopaedics, University of Maryland School of Medicine, 3Department of Diagnostic Radiology, University of Maryland School of Medicine

Date Published: May 11, 2011  |  doi:10.3791/2782  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Skeletal muscle, lengthening contraction, injury, regeneration, contractile function, torque

 JoVE Clinical and Translational Medicine

Microsurgical Venous Pouch Arterial-Bifurcation Aneurysms in the Rabbit Model: Technical Aspects


JoVE 2718

*1,2,3, *2,4, 2,4, 5, 6, 1, 2,4

1Department of Neurosurgery, Hospital Rudolfstiftung, 2Department of Neurosurgery, Kantonsspital Aarau, 3Cerebrovascular Research Group, Department of Biomedical Research, Medical University of Vienna, 4Cerebrovascular Research Group, Department of Intensive Care Medicine, University of Berne, 5Centre for Anatomy and Cell Biology, Department of Systematic Anatomy, Medical University of Vienna, 6Department of Neurology, Paracelsus University Salzburg

Date Published: May 11, 2011  |  doi:10.3791/2718  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, mental aneurysm, bifurcation, microsurgery, endovascular-coiling

 JoVE Clinical and Translational Medicine

Cannulation of the Mouse Submandibular Salivary Gland via the Wharton's Duct


JoVE 3074

1, 1, 1, 1, 1, 1, 2, 1

1Faculty of Dentistry, McGill University, 2National Institutes of Health, Bethesda, MD, USA

Date Published: May 14, 2011  |  doi:10.3791/3074  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Mouse, Salivary Gland, Wharton's Duct, dental disease, progenitor, stem cells

 JoVE Clinical and Translational Medicine

Gene Transfer for Ischemic Heart Failure in a Preclinical Model


JoVE 2778

, , , , ,

Cardiovascular Research Center, Mount Sinai School of Medicine

Date Published: May 15, 2011  |  doi:10.3791/2778  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Myocardial infarction, Gene therapy, Intracoronary injection, Viral vector, Ischemic heart failure

 JoVE Clinical and Translational Medicine

Magnetic Resonance Imaging Quantification of Pulmonary Perfusion using Calibrated Arterial Spin Labeling


JoVE 2712

1,2, 1,3, 1, 1, 3, 1, 3, 3, 1,3

1Medicine, University of California San Diego - UCSD, 2Bioengineering, University of California San Diego - UCSD, 3Radiology, University of California San Diego - UCSD

Date Published: May 30, 2011  |  doi:10.3791/2712  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, arterial spin labeling, lung proton density, functional lung imaging, hypoxic pulmonary vasoconstriction, oxygen consumption, ventilation, magnetic resonance imaging

 JoVE Clinical and Translational Medicine

Quantitative Analysis of Cancer Metastasis using an Avian Embryo Model


JoVE 2815

1, 2, 1

1Department of Pathology, Vanderbilt University, 2Departments of Oncology, Surgery and Medical Biophysics, London Regional cancer program

Date Published: May 30, 2011  |  doi:10.3791/2815  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, metastasis, chick embryo, Alu, chorioallantoic membrane (CAM), intravasation, cancer

 JoVE Bioengineering

Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor (IRIS)


JoVE 2694

1, 2, 2, 1, 2, 2, 3, 4,5, 4,5, 6, 6, 1, 5, 1,2

1Department of Electrical and Computer Engineering, Boston University, 2Department of Biomedical Engineering, Boston University, 3Center for Advanced Genomics Technology, Boston University, 4Department of Medicine, Section of Infectious Diseases, Boston University School of Medicine, 5Department of Microbiology, Boston University School of Medicine, 6CNR (National Research Council), Istituto di Chimica del Riconoscimento Molecolare

Date Published: May 3, 2011  |  doi:10.3791/2694  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Interferometry, label-free, biosensing, microarray, quantification, real-time detection

 JoVE Bioengineering

Three-dimensional Optical-resolution Photoacoustic Microscopy


JoVE 2729

, ,

Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis

Date Published: May 3, 2011  |  doi:10.3791/2729  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Optical-resolution photoacoustic microscopy, in vivo functional imaging, label-free imaging, noninvasive imaging, hemoglobin oxygen saturation, total hemoglobin concentration

 JoVE Bioengineering

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy


JoVE 2592

*1, *1, 1, 1,2

1Center for Biophotonics Science and Technology, University of California, Davis, 2Department of Pathology and Laboratory Medicine, University of California, Davis

Date Published: May 18, 2011  |  doi:10.3791/2592  |  Section: JoVE Bioengineering

Keywords: Microbiology, Raman scattering, all-optical gating, nonlinear optics, fluorescence, timeresolved spectroscopy.

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