Submit
Browse All

The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.

Recommend to Librarian
20132012201120102009200820072006
Table of Contents

Table of Contents: Issue 60, February 2012

Jump to:  JoVE Editorial (1) JoVE General (13) JoVE Neuroscience (13) JoVE Immunology and Infection (7) JoVE Clinical and Translational Medicine (9) JoVE Bioengineering (8)
 JoVE General

Investigating Intestinal Inflammation in DSS-induced Model of IBD


JoVE 3678

, , ,

Farncombe Family Digestive Health Research Institute, Department of Pathology and Molecular Medicine, McMaster University

Date Published: February 1, 2012  |  doi:10.3791/3678  |  Section: JoVE General

Keywords: Medicine, inflammation, myeloperoxidase (MPO), acute colonic damage, granulocyte, colon, dextran sulfate sodium (DSS), neutrophil

 JoVE General

Chromosomics: Detection of Numerical and Structural Alterations in All 24 Human Chromosomes Simultaneously Using a Novel OctoChrome FISH Assay


JoVE 3619

,

Genes and Environment Laboratory, University of California, Berkeley

Date Published: February 6, 2012  |  doi:10.3791/3619  |  Section: JoVE General

Keywords: Genetics, Chromosomics, OctoChrome-FISH, fluorescence in situ hybridization (FISH), Chromosome-wide aneuploidy study (CWAS), aneuploidy, chromosomal translocations, leukemia, lymphoma

 JoVE General

Pouring and Running a Protein Gel by reusing Commercial Cassettes


JoVE 3465

1, 1, 1, 1,2,3

1Department of Biology, University of Florida, 2UF Genetics Institute, University of Florida, 3Plant Molecular & Cellular Biology Program, University of Florida

Date Published: February 12, 2012  |  doi:10.3791/3465  |  Section: JoVE General

Keywords: Basic Protocols, Molecular Biology, minigel, cassettes, protein, gel, electrophoresis

 JoVE General

Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber


JoVE 3415

1, 2, 3

1Department of Physiology and Biophysics, Confocal Microscopy and Cell Imaging Core, Robert Wood Johnson Medical School, 2Department of Physiology and Biophysics, Robert Wood Johnson Medical School, 3Muscle Biology Research Group-MUBIG Schools of Nursing & Medicine, University of Missouri-Kansas City

Date Published: February 13, 2012  |  doi:10.3791/3415  |  Section: JoVE General

Keywords: Cellular Biology, Mn quenching, 2-APB, Fura-2, Orai1, esophageal squamous cell carcinoma, skinned muscle fiber

 JoVE General

A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica


JoVE 3265

1, 2

1Department of Biological Sciences, The University of Alabama, Huntsville, 2USDA-APHIS-PPQ, Center for Plant Health Science and Technology

Date Published: February 20, 2012  |  doi:10.3791/3265  |  Section: JoVE General

Keywords: Molecular Biology, Molecular genotyping, Japanese blood grass, Red Baron, cogongrass, invasive plants

 JoVE General

Selecting and Isolating Colonies of Human Induced Pluripotent Stem Cells Reprogrammed from Adult Fibroblasts


JoVE 3416

1,2, 1, 3, 3, 1, 1

1Department of Molecular Carcinogenesis and Center for Cancer Epigenetics, University of Texas M.D. Anderson Cancer Center, 2Department of Cell Biology, Poznan University of Medical Sciences, 3Department of Molecular Biology, The Scripps Research Institute

Date Published: February 20, 2012  |  doi:10.3791/3416  |  Section: JoVE General

Keywords: Developmental Biology, stem cells, induced pluripotent stem cells, iPSC, somatic cell reprogramming, pluripotency, retroviral transduction

 JoVE Neuroscience

Brain Imaging Investigation of the Impairing Effect of Emotion on Cognition


JoVE 2434

1,2, 1,3, 1, 4,5,6, 4,5, 6,7, 1,2,3,8,9

1Department of Psychiatry, University of Alberta, 2Centre for Neuroscience, University of Alberta, 3Department of Psychology, University of Illinois, 4Brain Imaging and Analysis Center, Duke University, 5Department of Psychiatry and Behavioral Sciences, Duke University, 6Mid-Atlantic Mental Illness Research Education and Clinical Center, VA Medical Center, 7Department of Psychology, Yale University, 8Neuroscience Program, University of Illinois, 9Beckman Institute for Advanced Science & Technology, University of Illinois

Date Published: February 1, 2012  |  doi:10.3791/2434  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Emotion-Cognition Interaction, Cognitive/Emotional Interference, Task-Irrelevant Distraction, Neuroimaging, fMRI, MRI

 JoVE Neuroscience

Culturing and Electrophysiology of Cells on NRCC Patch-clamp Chips


JoVE 3288

1, 2, 2, 2, 1, 3, 3, 2

1Institute for Microstructural Sciences, National Research Council of Canada, 2Institute for Biological Sciences, National Research Council of Canada, 3Hotchkiss Brain Institute, University of Calgary

Date Published: February 7, 2012  |  doi:10.3791/3288  |  Section: JoVE Neuroscience

Keywords: Neuroscience, disease models, pharmaceutical screens, electrophysiological recordings, patch-clamp, silicon planar patch-clamp chip, cultured neurons

 JoVE Neuroscience

Preterm EEG: A Multimodal Neurophysiological Protocol


JoVE 3774

1, 2, 3, 2,4, 1

1Department of Children's Clinical Neurophysiology, Helsinki University Hospital, University of Helsinki, 2Department of Biosciences, University of Helsinki, 3Department of Pediatrics, Helsinki University Hospital, University of Helsinki, 4Neuroscience Center, University of Helsinki

Date Published: February 18, 2012  |  doi:10.3791/3774  |  Section: JoVE Neuroscience

Keywords: Neuroscience, neurophysiology, preterm baby, neonatal, EEG, evoked response, high density EEG, FbEEG, sensory evoked response, neonatal intensive care unit

 JoVE Neuroscience

Morphometric Analyses of Retinal Sections


JoVE 3377

1, 1, 1, 1, 1,2,3, 1,2,3

1Laboratory of Neurodegenerative Diseases, Department of Anatomy, LKS Faculty of Medicine, The University of Hong Kong, 2Research Centre of Heart, Brain, Hormone and Healthy Aging, LKS Faculty of Medicine, The University of Hong Kong, 3State Key Laboratory of Brain and Cognitive Sciences, The University of Hong Kong

Date Published: February 19, 2012  |  doi:10.3791/3377  |  Section: JoVE Neuroscience

Keywords: Neuroscience, morphometric analysis, retina, thickness, cell size, Stereo Investigator, neuroscience

 JoVE Neuroscience

Detection of Microregional Hypoxia in Mouse Cerebral Cortex by Two-photon Imaging of Endogenous NADH Fluorescence


JoVE 3466

1, 2, 2, 1, 1, 3

1Department of Microbiology and Immunology, University of Rochester Medical Center, 2Center for Neural Development and Disease, University of Rochester Medical Center, 3Deptartment of Neurology, Center for Neural Development and Disease, University of Rochester Medical Center

Date Published: February 21, 2012  |  doi:10.3791/3466  |  Section: JoVE Neuroscience

Keywords: Neuroscience, mouse, two-photon, cortex, nicotinamide adenine dinucleotide, angiography, hypoxia

 JoVE Neuroscience

Surgical Implantation of Chronic Neural Electrodes for Recording Single Unit Activity and Electrocorticographic Signals


JoVE 3565

1, 1, 2, 2, 2, 1,3

1Biomedical Engineering, University of Michigan, 2Biomedical Engineering, University of Wisconsin-Madison, 3NeuroNexus Technologies

Date Published: February 24, 2012  |  doi:10.3791/3565  |  Section: JoVE Neuroscience

Keywords: Neuroscience, chronic, silicon electrode, thin film surface electrode, microECoG, surgery, survival, electrophysiology

 JoVE Neuroscience

Voltage Biasing, Cyclic Voltammetry, & Electrical Impedance Spectroscopy for Neural Interfaces


JoVE 3566

1, 2, 2, 3, 2, 1,4

1Weldon School of Biomedical Engineering, Purdue University, 2Biomedical Engineering, University of Wisconsin-Madison, 3Biomedical Engineering, University of Michigan, 4Department of Biological Sciences, Purdue University

Date Published: February 24, 2012  |  doi:10.3791/3566  |  Section: JoVE Neuroscience

Keywords: Neuroscience, neuroprosthesis, electrode-tissue interface, rejuvenation, neural engineering, neuroscience, neural implant, electrode, brain-computer interface, electrochemistry

 JoVE Neuroscience

T-maze Forced Alternation and Left-right Discrimination Tasks for Assessing Working and Reference Memory in Mice


JoVE 3300

1,2, 1, 3, 1,2,3, 1,2,3

1Division of Systems Medical Science, Institute for Comprehensive Medical Science, Fujita Health University, 2Japan Science and Technology Agency, Core Research for Evolutionary Science and Technology (CREST), 3Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, National Institutes of Natural Sciences

Date Published: February 26, 2012  |  doi:10.3791/3300  |  Section: JoVE Neuroscience

Keywords: Neuroscience, T-maze, learning, memory, behavioral flexibility, behavior, mouse

 JoVE Neuroscience

Enabling High Grayscale Resolution Displays and Accurate Response Time Measurements on Conventional Computers


JoVE 3312

1, 2,3,4,5

1Center for Cognitive and Behavioral Brain Imaging, The Ohio State University, 2Department of Psychology, University of Southern California, 3Biomedical Engineering, University of Southern California, 4Neuroscience Graduate Program, University of Southern California, 5Department of Psychology, The Ohio State University

Date Published: February 29, 2012  |  doi:10.3791/3312  |  Section: JoVE Neuroscience

Keywords: Neuroscience, VideoSwitcher, Visual stimulus, Luminance resolution, Contrast, Trigger, RTbox, Response time

 JoVE Neuroscience

Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching


JoVE 3747

, , ,

MRC Centre for Synaptic Plasticity, University of Bristol

Date Published: February 29, 2012  |  doi:10.3791/3747  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Fluorescence Recovery After Photobleaching, FRAP, Confocal imaging, fluorophore, GFP, Super-ecliptic pHluorin, SEP, fluorescence loss in photobleach, FLIP, neuron, protein traffic, synapse

 JoVE Immunology and Infection

High-throughput Detection Method for Influenza Virus


JoVE 3623

1, 1, 2, 3, 4, 4, 1,5, 1,6

1Laboratory of Molecular Immunology and Immunotherapy, Blood Research Institute, 2Department of Microbiology, Mount Sinai School of Medicine, 3Laboratory of Molecular Genetics, Blood Research Institute, 4City of Milwaukee Health Department Laboratory, 5Division of Hematology-Oncology/BMT, Children's Hospital of Wisconsin, Medical College of Wisconsin, 6Division of Hematology and Oncology, Dept Medicine, Medical College of Wisconsin

Date Published: February 4, 2012  |  doi:10.3791/3623  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Influenza virus, Virus titer, Epithelial cells

 JoVE Immunology and Infection

Intravital Imaging of the Mouse Popliteal Lymph Node


JoVE 3720

1, 1, 1, 2

1Department of Pediatrics, Case Western Reserve University, 2Department of Pediatrics, Pathology and Biomedical Engineering, Case Western Reserve University

Date Published: February 8, 2012  |  doi:10.3791/3720  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Lymph node, popliteal, intravital, multi-photon, microscopy, cell trafficking, mouse, tumor immunology

 JoVE Immunology and Infection

Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry


JoVE 3796

, , ,

Institute for Molecular Infection Biology (IMIB), University of Würzburg

Date Published: February 15, 2012  |  doi:10.3791/3796  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Bacillus subtilis, biofilm formation, gene expression, cell differentiation, single-cell analysis

 JoVE Immunology and Infection

Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks


JoVE 3894

1, 2, 1, 2, 2, 1

1Microbiology and Pathogenesis Activity, Bacterial Diseases Branch, Division of Vector-Borne Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, 2Tick-Borne Diseases Activity, Bacterial Diseases Branch, Division of Vector-Borne Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention

Date Published: February 21, 2012  |  doi:10.3791/3894  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Ixodes scapularis, Lyme disease, Borrelia burgdorferi, salivary glands, hemolymph, tick dissection, saliva, tick

 JoVE Immunology and Infection

Generation of Organotypic Raft Cultures from Primary Human Keratinocytes


JoVE 3668

1, 1,2

1Department of Microbiology & Immunology, University of North Carolina-Chapel Hill, 2Lineberger Cancer Center, University of North Carolina-Chapel Hill

Date Published: February 22, 2012  |  doi:10.3791/3668  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Epithelium, organotypic raft culture, virus, keratinocytes, papillomavirus

 JoVE Clinical and Translational Medicine

Spectral Karyotyping to Study Chromosome Abnormalities in Humans and Mice with Polycystic Kidney Disease


JoVE 3887

1, 2, 1

1Department of Pharmacology, University of Toledo, College of Pharmacy and Pharmaceutical Sciences, 2Department of Emergency and Intensive Care, ProMedica Sponsored Research

Date Published: February 3, 2012  |  doi:10.3791/3887  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Chromosome, Polycystic Kidney Disease, Primary Cilia, Spectral Karyotyping, Cytogenetics

 JoVE Clinical and Translational Medicine

Retrograde Perfusion and Filling of Mouse Coronary Vasculature as Preparation for Micro Computed Tomography Imaging


JoVE 3740

1, 1, 1,2, 1, 1

1Department of Pathology, Center for Cardiovascular Biology, and Institute for Stem Cell and Regenerative Medicine, University of Washington, 2Departments of Bioengineering and Medicine/Cardiology, University of Washington

Date Published: February 10, 2012  |  doi:10.3791/3740  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Vascular biology, heart, coronary vessels, mouse, micro Computed Tomography (μCT) imaging, Microfil

 JoVE Clinical and Translational Medicine

MALDI Imaging Mass Spectrometry of Neuropeptides in Parkinson's Disease


JoVE 3445

1,2, 1, 1

1Department of Pharmaceutical Biosciences, Uppsala University, 2Department of Chemical and Biological Engineering, Chalmers University of Technology

Date Published: February 14, 2012  |  doi:10.3791/3445  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Parkinson's disease, L-DOPA induced dyskinesia, striatum, opioid peptides, MALDI Imaging MS

 JoVE Clinical and Translational Medicine

MAME Models for 4D Live-cell Imaging of Tumor: Microenvironment Interactions that Impact Malignant Progression


JoVE 3661

1, 1, 1, 1,2, 1,2, 1,2

1Department of Pharmacology, Wayne State University, 2Barbara Ann Karmanos Cancer Institute, Wayne State University

Date Published: February 17, 2012  |  doi:10.3791/3661  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Immunology, Breast, cancer, extracellular matrix, invasion, proteolysis, tumor microenvironment

 JoVE Clinical and Translational Medicine

The WATCHMAN Left Atrial Appendage Closure Device for Atrial Fibrillation


JoVE 3671

, , , , ,

University of Leipzig Heart Center

Date Published: February 28, 2012  |  doi:10.3791/3671  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, atrial fibrillation, cardiology, cardiac, interventional cardiology, medical procedures, medicine, WATCHMAN, medical device, left atrial appendage

 JoVE Clinical and Translational Medicine

A New Single Chamber Implantable Defibrillator with Atrial Sensing: A Practical Demonstration of Sensing and Ease of Implantation


JoVE 3750

, , ,

Heart Center Rostock, University Hospital of Rostock, Germany

Date Published: February 28, 2012  |  doi:10.3791/3750  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Implantable defibrillator, dual chamber, single chamber, tachycardia detection

 JoVE Bioengineering

Magnetic Resonance Elastography Methodology for the Evaluation of Tissue Engineered Construct Growth


JoVE 3618

1, 1, 1, 2, 1

1Department of Biological Systems Engineering, University of Nebraska-Lincoln, 2Department of Engineering Mechanics, University of Nebraska-Lincoln

Date Published: February 9, 2012  |  doi:10.3791/3618  |  Section: JoVE Bioengineering

Keywords: Bioengineering, mesenchymal stem cells, tissue engineering (TE), regenerative medicine, adipose TE, magnetic resonance elastography (MRE), biomechanics, elasticity

 JoVE Bioengineering

Electric Field-controlled Directed Migration of Neural Progenitor Cells in 2D and 3D Environments


JoVE 3453

*1, *2,3, 1, 3, 3, 3, 1

1School of Dentistry, Cardiff Institute of Tissue Engineering & Repair, Cardiff University, 2Shandong Qianfoshan Hospital, Shandong University School of Medicine, 3Dermatology and Ophthalmology Research, Institute for Regenerative Cures, University of California at Davis

Date Published: February 16, 2012  |  doi:10.3791/3453  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Electric field, neural progenitor cells, cell migration, spinal cord slice, ex vivo tracking, galvanotaxis, electrotaxis

Waiting
simple hit counter