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Table of Contents

Table of Contents: Issue 71, January 2013

Jump to:  JoVE Editorial (1) JoVE Biology (15) JoVE Neuroscience (13) JoVE Immunology and Infection (6) JoVE Clinical and Translational Medicine (10) JoVE Bioengineering (4) JoVE Applied Physics (4) JoVE Errata (1)
 JoVE Biology

Assessment of Mitochondrial Functions and Cell Viability in Renal Cells Overexpressing Protein Kinase C Isozymes

1Department of Pharmaceutical Sciences, College of Pharmacy, University of Arkansas for Medical Sciences


JoVE 4301

Date Published: January 7, 2013  |  doi:10.3791/4301  |  Section: JoVE Biology

 JoVE Biology

Measurement of Lifespan in Drosophila melanogaster

1Department of Molecular and Integrative Physiology, University of Michigan, 2Cellular and Molecular Biology Program, University of Michigan


JoVE 50068

Date Published: January 7, 2013  |  doi:10.3791/50068  |  Section: JoVE Biology

 JoVE Biology

The Logic, Experimental Steps, and Potential of Heterologous Natural Product Biosynthesis Featuring the Complex Antibiotic Erythromycin A Produced Through E. coli

1Chemical and Biological Engineering Department, State University of New York at Buffalo, 2Chemical Engineering Department, Massachusetts Institute of Technology


JoVE 4346

Date Published: January 13, 2013  |  doi:10.3791/4346  |  Section: JoVE Biology

 JoVE Biology

Metabolic Profile Analysis of Zebrafish Embryos

1Metabolic Research Unit & Molecular & Medical Research SRC, Geelong, Australia, School of Medicine, Deakin University


JoVE 4300

Date Published: January 14, 2013  |  doi:10.3791/4300  |  Section: JoVE Biology

 JoVE Biology

Isolation of Normal and Cancer-associated Fibroblasts from Fresh Tissues by Fluorescence Activated Cell Sorting (FACS)

1Department of Pathology, Sackler School of Medicine, Tel Aviv University


JoVE 4425

Date Published: January 14, 2013  |  doi:10.3791/4425  |  Section: JoVE Biology

 JoVE Biology

An Explant Assay for Assessing Cellular Behavior of the Cranial Mesenchyme

1Center for Neuroscience Research, Children's Research Institute, Children's National Medical Center


JoVE 4245

Date Published: January 20, 2013  |  doi:10.3791/4245  |  Section: JoVE Biology

 JoVE Biology

LeafJ: An ImageJ Plugin for Semi-automated Leaf Shape Measurement

1Department of Plant Biology, University of California Davis


JoVE 50028

Date Published: January 21, 2013  |  doi:10.3791/50028  |  Section: JoVE Biology

 JoVE Biology

Super-resolution Imaging of the Bacterial Division Machinery

1Department of Biophysics and Biophysical Chemistry, The Johns Hopkins University School of Medicine


JoVE 50048

Date Published: January 21, 2013  |  doi:10.3791/50048  |  Section: JoVE Biology

 JoVE Biology

Blastomere Explants to Test for Cell Fate Commitment During Embryonic Development

1Department of Biological Sciences, The George Washington University, 2Department of Anatomy and Regenerative Biology, The George Washington University


JoVE 4458

Date Published: January 26, 2013  |  doi:10.3791/4458  |  Section: JoVE Biology

 JoVE Biology

High-throughput Screening for Small-molecule Modulators of Inward Rectifier Potassium Channels

1Department of Pharmacology, Vanderbilt University School of Medicine, 2Department of Anesthesiology, Vanderbilt University School of Medicine, 3Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine


JoVE 4209

Date Published: January 27, 2013  |  doi:10.3791/4209  |  Section: JoVE Biology

 JoVE Biology

Genetic Modification and Recombination of Salivary Gland Organ Cultures

1Department of Biological Sciences, University at Albany, SUNY


JoVE 50060

Date Published: January 28, 2013  |  doi:10.3791/50060  |  Section: JoVE Biology

 JoVE Biology

Real-time Analyses of Retinol Transport by the Membrane Receptor of Plasma Retinol Binding Protein

1Department of Physiology, Jules Stein Eye Institute and Howard Hughes Medical Institute, University of California, Los Angeles


JoVE 50169

Date Published: January 28, 2013  |  doi:10.3791/50169  |  Section: JoVE Biology

 JoVE Biology

Laser-inflicted Injury of Zebrafish Embryonic Skeletal Muscle

1Max Delbrück Center for Molecular Medicine


JoVE 4351

Date Published: January 30, 2013  |  doi:10.3791/4351  |  Section: JoVE Biology

 JoVE Biology

Whole Mount RNA Fluorescent in situ Hybridization of Drosophila Embryos

1Institut de Recherches Cliniques de Montréal (IRCM), 2Department of Biochemistry, Université de Montréal


JoVE 50057

Date Published: January 30, 2013  |  doi:10.3791/50057  |  Section: JoVE Biology

 JoVE Biology

Isometric and Eccentric Force Generation Assessment of Skeletal Muscles Isolated from Murine Models of Muscular Dystrophies

1Department of Anatomy and Cell Biology, School of Dental Medicine, University of Pennsylvania, 2Department of Physiology, Perelman School of Medicine, University of Pennsylvania, 3Department of Anatomy and Cell Biology, School of Dental Medicine, School of Dental Medicine, University of Pennsylvania


JoVE 50036

Date Published: January 31, 2013  |  doi:10.3791/50036  |  Section: JoVE Biology

 JoVE Neuroscience

Intravascular Perfusion of Carbon Black Ink Allows Reliable Visualization of Cerebral Vessels

1Department of Neurology, University of Duisburg-Essen Medical School


JoVE 4374

Date Published: January 4, 2013  |  doi:10.3791/4374  |  Section: JoVE Neuroscience

 JoVE Neuroscience

The Dig Task: A Simple Scent Discrimination Reveals Deficits Following Frontal Brain Damage

1Department of Psychology, Southern Illinois University at Carbondale


JoVE 50033

Date Published: January 4, 2013  |  doi:10.3791/50033  |  Section: JoVE Neuroscience

 JoVE Neuroscience

In Vivo Two-photon Imaging Of Experience-dependent Molecular Changes In Cortical Neurons

1Unit on Neural Circuits and Adaptive Behaviors, Genes Cognition and Psychosis Program, National Institute of Mental Health, 2Department of Neuroscience, Brown University - National Institutes of Health Graduate Partnership Program, 3Section on Synaptic Pharmacology, Laboratory for Integrative Neuroscience, National Institute on Alcohol Abuse and Alcoholism, 4Champalimaud Neuroscience Programme, Champalimaud Center for the Unknown


JoVE 50148

Date Published: January 5, 2013  |  doi:10.3791/50148  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Sequential Photo-bleaching to Delineate Single Schwann Cells at the Neuromuscular Junction

1Lehrstuhl für Biomolekulare Sensoren, Technische Universität München, 2Center for Integrated Protein Science (Munich) at the Institute of Neuroscience, Technische Universität München, 3TUM Institute for Advanced Study and German Center for Neurodegenerative Diseases, Technische Universität München, 4Munich Cluster for Systems Neurology (SyNergy), Technische Universität München


JoVE 4460

Date Published: January 11, 2013  |  doi:10.3791/4460  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Hippocampal Insulin Microinjection and In vivo Microdialysis During Spatial Memory Testing

1Behavioral Neuroscience, University at Albany


JoVE 4451

Date Published: January 11, 2013  |  doi:10.3791/4451  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Peering into the Dynamics of Social Interactions: Measuring Play Fighting in Rats

1Department of Neuroscience, University of Lethbridge


JoVE 4288

Date Published: January 18, 2013  |  doi:10.3791/4288  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Manufacturing and Using Piggy-back Multibarrel Electrodes for In vivo Pharmacological Manipulations of Neural Responses

1Department of Physiology & Biophysics, University of Colorado Medical Campus


JoVE 4358

Date Published: January 18, 2013  |  doi:10.3791/4358  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Isolation and Culture of Mouse Cortical Astrocytes

1Institute of Anatomy and Cell Biology, University of Freiburg, 2Centre of Chronic Immunodeficiency (CCI), University Medical Centre Freiburg, University of Freiburg


JoVE 50079

Date Published: January 19, 2013  |  doi:10.3791/50079  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Forebrain Electrophysiological Recording in Larval Zebrafish

1Epilepsy Research Laboratory, Department of Neurological Surgery, University of California, San Francisco


JoVE 50104

Date Published: January 24, 2013  |  doi:10.3791/50104  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Recording and Analysis of Circadian Rhythms in Running-wheel Activity in Rodents

1Douglas Mental Health University Institute, Department of Psychiatry, McGill University, 2Center for Studies in Behavioral Neurobiology, Department of Psychology, Concordia University


JoVE 50186

Date Published: January 24, 2013  |  doi:10.3791/50186  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Multi-unit Recording Methods to Characterize Neural Activity in the Locust (Schistocerca Americana) Olfactory Circuits

1Department of Biomedical Engineering, Washington University in St. Louis


JoVE 50139

Date Published: January 25, 2013  |  doi:10.3791/50139  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Optogenetic Stimulation of Escape Behavior in Drosophila melanogaster

1Department of Neurobiology, Stanford University


JoVE 50192

Date Published: January 25, 2013  |  doi:10.3791/50192  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Optogenetic Activation of Zebrafish Somatosensory Neurons using ChEF-tdTomato

1Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles


JoVE 50184

Date Published: January 31, 2013  |  doi:10.3791/50184  |  Section: JoVE Neuroscience

 JoVE Immunology and Infection

Antigens Protected Functional Red Blood Cells By The Membrane Grafting Of Compact Hyperbranched Polyglycerols

1Centre for Blood Research, University of British Columbia, 2Department of Pathology and Laboratory Medicine, University of British Columbia, 3Canadian Blood Services, University of British Columbia, 4Department of Chemistry, Life Sciences Centre, University of British Columbia


JoVE 50075

Date Published: January 2, 2013  |  doi:10.3791/50075  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Isolation and Analysis of Brain-sequestered Leukocytes from Plasmodium berghei ANKA-infected Mice

1The Walter and Eliza Hall Institute of Medical Research


JoVE 50112

Date Published: January 2, 2013  |  doi:10.3791/50112  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

A Simple Chelex Protocol for DNA Extraction from Anopheles spp.

1Malaria Institute at Macha, 2Department of Molecular Microbiology & Immunology, Johns Hopkins Bloomberg School of Public Health


JoVE 3281

Date Published: January 9, 2013  |  doi:10.3791/3281  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Live-cell Video Microscopy of Fungal Pathogen Phagocytosis

1Division of Applied Medicine, University of Aberdeen, 2Aberdeen Fungal Group, University of Aberdeen


JoVE 50196

Date Published: January 9, 2013  |  doi:10.3791/50196  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Right Ventricular Systolic Pressure Measurements in Combination with Harvest of Lung and Immune Tissue Samples in Mice

1Department of Environmental Medicine, New York University School of Medicine, Tuxedo, 2Division of Allergy, Pulmonary, & Critical Care Medicine, Department of Medicine, Vanderbilt University Medical Center, 3Division of Pulmonary Medicine, New York University School of Medicine


JoVE 50023

Date Published: January 16, 2013  |  doi:10.3791/50023  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Measurement of Tactile Allodynia in a Murine Model of Bacterial Prostatitis

1Department of Urology, Northwestern University Feinberg School of Medicine


JoVE 50158

Date Published: January 16, 2013  |  doi:10.3791/50158  |  Section: JoVE Immunology and Infection

 JoVE Clinical and Translational Medicine

Immunohistochemical Staining of B7-H1 (PD-L1) on Paraffin-embedded Slides of Pancreatic Adenocarcinoma Tissue

1The Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University School of Medicine, 2Department of Oncology, The Johns Hopkins University School of Medicine, 3Department of Dermatology, The Johns Hopkins University School of Medicine, 4Department of Surgery, Johns Hopkins University School of Medicine, 5The Sol Goldman Pancreatic Cancer Center, The Johns Hopkins University School of Medicine, 6Yale Cancer Center, Yale School of Medicine, 7The Skip Viragh Center for Pancreatic Cancer, The Johns Hopkins University School of Medicine, 8Department of Pathology, The Johns Hopkins University School of Medicine


JoVE 4059

Date Published: January 3, 2013  |  doi:10.3791/4059  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Processing of Human Reduction Mammoplasty and Mastectomy Tissues for Cell Culture

1Life Science Division, Lawrence Berkeley National Laboratory


JoVE 50011

Date Published: January 3, 2013  |  doi:10.3791/50011  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Improved Protocol For Laser Microdissection Of Human Pancreatic Islets From Surgical Specimens

1Molecular Diabetology, Paul Langerhans Institute Dresden, 2Department of GI-, Thoracic- and Vascular Surgery, University Hospital Carl Gustav Carus, University of Technology Dresden, 3Department of Endocrinology and Metabolism, Metabolic Unit University of Pisa, 4Labs DC0522, Lilly Corporate Center, 5Genomics, Faculty of Medicine Imperial College London, 6Vital-IT, SIB Swiss Institute of Bioinformatics, 7Clinical Biochemistry, Hannover Medical School, 8Cell Physiology and Metabolism, Medical School, University of Geneva, 9Department of Pathology, University Hospital Carl Gustav Carus, University of Technology Dresden, 10R&D DIAB Division / Translational Medicine, Sanofi-Aventis


JoVE 50231

Date Published: January 6, 2013  |  doi:10.3791/50231  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Probe-based Confocal Laser Endomicroscopy of the Urinary Tract: The Technique

1Department of Urology, Stanford University School of Medicine, 2Veterans Affairs Palo Alto Health Care System


JoVE 4409

Date Published: January 10, 2013  |  doi:10.3791/4409  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Breathing-controlled Electrical Stimulation (BreEStim) for Management of Neuropathic Pain and Spasticity

1Department of Physical Medicine and Rehabilitation, University of Texas Health Science Center at Houston, 2UTHealth Motor Recovery Laboratory, TIRR Memorial Hermann Hospital, 3The Institute of Rehabilitation and Research (TIRR), TIRR Memorial Hermann Hospital


JoVE 50077

Date Published: January 10, 2013  |  doi:10.3791/50077  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research

1Umeå Centre for Molecular Medicine, Umeå University, 2Cell Transplant Center, Diabetes Research Institute, University of Miami,, 3EMBL-CRG Systems Biology Program, Centre for Genomic Regulation, Catalan Institute of Research and Advanced Studies, 4Dept. of Computing Science, Umeå University


JoVE 50238

Date Published: January 12, 2013  |  doi:10.3791/50238  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

A Low Mortality Rat Model to Assess Delayed Cerebral Vasospasm After Experimental Subarachnoid Hemorrhage

1Department of Neurosurgery, SUNY Upstate Medical University, 2Department of Neuroscience and Physiology, SUNY Upstate Medical University


JoVE 4157

Date Published: January 17, 2013  |  doi:10.3791/4157  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Movement Retraining using Real-time Feedback of Performance

1Department of Physical Therapy, University of British Columbia


JoVE 50182

Date Published: January 17, 2013  |  doi:10.3791/50182  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

The Use of Reverse Phase Protein Arrays (RPPA) to Explore Protein Expression Variation within Individual Renal Cell Cancers

1Edinburgh Urological Cancer Group, University of Edinburgh, 2School of Medicine, University of St Andrews, 3Division of Pathology, University of Edinburgh, 4MRC Human Genetics Unit, MRC IGMM, University of Edinburgh, 5Department of Pathology, Western General Hospital, 6Breakthrough Breast Cancer Research Unit, University of Edinburgh, 7St Bartholomew's Cancer Institute, Experimental Cancer Medicine Centre, Queen Mary University of London


JoVE 50221

Date Published: January 22, 2013  |  doi:10.3791/50221  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Non-invasive Assessment of Microvascular and Endothelial Function

1Department of Family and Community Medicine, Thomas Jefferson University, 2Department of Pharmacology and Experimental Therapeutics, Biostatistics Division, Thomas Jefferson University, 3Department of Internal Medicine, Thomas Jefferson University


JoVE 50008

Date Published: January 29, 2013  |  doi:10.3791/50008  |  Section: JoVE Clinical and Translational Medicine

 JoVE Bioengineering

Small and Wide Angle X-Ray Scattering Studies of Biological Macromolecules in Solution

1Department of Mechanical, Aerospace, and Nuclear Engineering, Rensselaer Polytechnic Institute


JoVE 4160

Date Published: January 8, 2013  |  doi:10.3791/4160  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Patient-specific Modeling of the Heart: Estimation of Ventricular Fiber Orientations

1Institute for Computational Medicine and the Department of Biomedical Engineering, Johns Hopkins University


JoVE 50125

Date Published: January 8, 2013  |  doi:10.3791/50125  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

1Department of Biomedical Engineering, Northwestern University, 2Department of Physics, Harbin Institute of Technology, 3Department of Ophthalmology, University of Southern California, 4Department of Ophthalmology, Northwestern University


JoVE 4390

Date Published: January 15, 2013  |  doi:10.3791/4390  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Optical Frequency Domain Imaging of Ex vivo Pulmonary Resection Specimens: Obtaining One to One Image to Histopathology Correlation

1Department of Pathology, Harvard Medical School, 2Massachusetts General Hospital, 3Wellman Center for Photomedicine, Harvard Medical School, 4Pulmonary and Critical Care Unit, Massachusetts General Hospital, 5Pulmonary and Critical Care Unit, Harvard Medical School


JoVE 3855

Date Published: January 22, 2013  |  doi:10.3791/3855  |  Section: JoVE Bioengineering

 JoVE Applied Physics

Optical Trapping of Nanoparticles

1Electrical and Computer Engineering, University of Victoria


JoVE 4424

Date Published: January 15, 2013  |  doi:10.3791/4424  |  Section: JoVE Applied Physics

 JoVE Applied Physics

Nanomoulding of Functional Materials, a Versatile Complementary Pattern Replication Method to Nanoimprinting

1Institute of Microengineering (IMT), Photovoltaics and Thin Film Electronics Laboratory, Ecole Polytechnique Fédérale de Lausanne (EPFL), 2Department of Electrical Engineering and Computer Sciences, University of California, Berkeley


JoVE 50177

Date Published: January 23, 2013  |  doi:10.3791/50177  |  Section: JoVE Applied Physics

 JoVE Applied Physics

Concurrent Quantitative Conductivity and Mechanical Properties Measurements of Organic Photovoltaic Materials using AFM

1Center for Nanoscale Materials, Argonne National Laboratory, 2Institute for Molecular Engineering, University of Chicago


JoVE 50293

Date Published: January 23, 2013  |  doi:10.3791/50293  |  Section: JoVE Applied Physics

 JoVE Applied Physics

Development of Whispering Gallery Mode Polymeric Micro-optical Electric Field Sensors

1Mechanical Engineering Department, Southern Methodist University


JoVE 50199

Date Published: January 29, 2013  |  doi:10.3791/50199  |  Section: JoVE Applied Physics

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