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The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.

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Table of Contents

Table of Contents: Issue 56, October 2011

Jump to:  JoVE Editorial (1) JoVE General (17) JoVE Neuroscience (10) JoVE Immunology and Infection (6) JoVE Clinical and Translational Medicine (10) JoVE Bioengineering (8)
 JoVE General

Experimental Manipulation of Body Size to Estimate Morphological Scaling Relationships in Drosophila


JoVE 3162

1, 2, 2, 1

1Department of Biology & Biochemistry, University of Houston, 2Department of Zoology, Michigan State University

Date Published: October 1, 2011  |  doi:10.3791/3162  |  Section: JoVE General

Keywords: Developmental Biology, Drosophila, allometry, morphology, body size, scaling, insect

 JoVE General

Isolation of CD133+ Liver Stem Cells for Clonal Expansion


JoVE 3183

1, 1, 1, 2, 3

1Department of Pediatrics and Pharmacology, Pennsylvania State College of Medicine, 2Department of Pharmacology, Pennsylvania State College of Medicine, 3Department of Pediatrics, University of California Los Angeles, School of Medicine

Date Published: October 10, 2011  |  doi:10.3791/3183  |  Section: JoVE General

Keywords: Developmental Biology, CD133, liver stem cell, oval cell, liver cancer stem cell, stem cell, cell isolation, non-parenchymal fraction of liver, flow cytometry

 JoVE General

Tracking Morphogenetic Tissue Deformations in the Early Chick Embryo


JoVE 3129

1, 1, 1,2, 1,3

1Department of Biomedical Engineering, Washington University, 2Institute for Information Transmission Problems, Russian Academy of Sciences, 3Department of Mechanical Engineering and Materials Science, Washington University

Date Published: October 17, 2011  |  doi:10.3791/3129  |  Section: JoVE General

Keywords: Developmental Biology, biomechanics, labeling, chick embryo, morphogenesis, time-lapse imaging, optical coherence tomography, strain analysis

 JoVE General

Monitoring Equilibrium Changes in RNA Structure by 'Peroxidative' and 'Oxidative' Hydroxyl Radical Footprinting


JoVE 3244

*1, *2, 2, 2, 2

1Department of Chemistry, Hunter College, 2Department of Biochemistry, Albert Einstein College of Medicine

Date Published: October 17, 2011  |  doi:10.3791/3244  |  Section: JoVE General

Keywords: Molecular Biology, hydroxyl radical, footprinting, RNA, Fenton, equilibrium

 JoVE General

Robust Generation of Hepatocyte-like Cells from Human Embryonic Stem Cell Populations


JoVE 2969

, , , ,

Medical Research Council Centre for Regenerative Medicine, University of Edinburgh

Date Published: October 26, 2011  |  doi:10.3791/2969  |  Section: JoVE General

Keywords: Developmental Biology, Stem Cells, hESC, Development, Endoderm, Liver, Hepatocyte, Endocrine Function, Exocrine Function

 JoVE General

Isolation & Characterization of Hoechstlow CD45negative Mouse Lung Mesenchymal Stem Cells


JoVE 3159

1,2, 1,2, 3, 1,2,4, 1,2,3

1Charles C. Gates Regenerative Medicine and Stem Cell Biology Program, University of Colorado Denver, 2Department of Medicine, University of Colorado Denver, 3Cancer Center, University of Colorado Denver, 4Webb Waring Institute, University of Colorado Denver

Date Published: October 26, 2011  |  doi:10.3791/3159  |  Section: JoVE General

Keywords: Developmental Biology, mesenchymal stem cell, MSC, lung stem cell, resident lung mesenchymal stem cells, stem cells

 JoVE General

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique


JoVE 3255

1, 1,2

1Department of Molecular Oncology, Weatherall Institute of Molecular Medicine, University of Oxford, 2Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw

Date Published: October 27, 2011  |  doi:10.3791/3255  |  Section: JoVE General

Keywords: Molecular Biology, Genetics, DNA fiber analysis, replication speed, fork stalling, origin firing, termination

 JoVE General

Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA


JoVE 3340

1, 1, 1, 1, 1,2,3, 1, 1

1Regenerative Biology, Morgridge Institute for Research, 2Department of Cell & Regenerative Biology, University of Wisconsin, 3Department of Molecular, Cellular, & Regenerative Biology, University of California

Date Published: October 27, 2011  |  doi:10.3791/3340  |  Section: JoVE General

Keywords: Molecular Biology, Genetics, mRNA-Seq, Illumina-Seq, gene expression profiling, high throughput sequencing

 JoVE Neuroscience

Mapping Inhibitory Neuronal Circuits by Laser Scanning Photostimulation


JoVE 3109

1, 1, 1, 1,2

1Department of Anatomy and Neurobiology, School of Medicine, University of California, Irvine, 2Department of Biomedical Engineering, School of Engineering, University of California, Irvine

Date Published: October 6, 2011  |  doi:10.3791/3109  |  Section: JoVE Neuroscience

Keywords: Neuroscience, glutamate uncaging, whole cell recording, GFP, transgenic, interneurons

 JoVE Neuroscience

Using MazeSuite and Functional Near Infrared Spectroscopy to Study Learning in Spatial Navigation


JoVE 3443

1, 1,2, 1, 1, 1, 1

1School of Biomedical Engineering, Science and Health Systems, Drexel University, 2College of Nursing and Health Professions, Drexel University

Date Published: October 8, 2011  |  doi:10.3791/3443  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Cognitive, optical, brain, imaging, functional near-infrared spectroscopy, fNIR, spatial, navigation, software

 JoVE Neuroscience

VisioTracker, an Innovative Automated Approach to Oculomotor Analysis


JoVE 3556

1, 2, 2, 1

1Institute of Molecular Life Sciences, University of Zurich, 2TSE Systems GmbH

Date Published: October 12, 2011  |  doi:10.3791/3556  |  Section: JoVE Neuroscience

Keywords: Neuroscience, zebrafish, fish larvae, visual system, optokinetic response, developmental genetics, pharmacology, mutants, Danio rerio, adult fish

 JoVE Neuroscience

Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction


JoVE 3273

1,2, 3,4, 1,2, 1,2,5, 1,2,6, 1,2

1San Diego Regenerative Medicine Institute, 2Xcelthera, 3Department of Neurosurgery, Harvard Medical School, 4Division of SCI Research, VA Boston Healthcare System, 5Program in Stem Cell & Regenerative Biology, Sanford-Burnham Medical Research Institute, 6La Jolla IVF

Date Published: October 28, 2011  |  doi:10.3791/3273  |  Section: JoVE Neuroscience

Keywords: Neuroscience, stem cell, human embryonic stem cell, human, neuronal progenitor, neuron, human pluripotent cell, neuronal differentiation, small molecule induction, cell culture, cell therapy

 JoVE Neuroscience

Isolation and Expansion of Human Glioblastoma Multiforme Tumor Cells Using the Neurosphere Assay


JoVE 3633

1,2, 1, 1, 1, 1, 1

1Department of Neurosurgery, University of Florida, 2Department of Anatomical Sciences, Shiraz University of Medical Sciences

Date Published: October 30, 2011  |  doi:10.3791/3633  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Glioblastoma Multiforme, Tumor Cell, Neurosphere Assay, Isolation, Expansion

 JoVE Neuroscience

Imaging Odor-Evoked Activities in the Mouse Olfactory Bulb using Optical Reflectance and Autofluorescence Signals


JoVE 3336

, , , , , ,

Laboratoire d’Imagerie et de Modélisation en Neurobiologie et Cancérologie, UMR8165 Université Paris Sud 11, Paris Diderot 7 – CNRS

Date Published: October 31, 2011  |  doi:10.3791/3336  |  Section: JoVE Neuroscience

Keywords: Neuroscience, wide-field optical imaging, flavoproteins, hemodynamics, olfactory bulb, sensory activity, mice

 JoVE Immunology and Infection

Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus


JoVE 3140

1, 2

1Center for Autophagy Research, Department of Internal Medicine, UT Southwestern Medical Center, 2Department of Microbiology, UT Southwestern Medical Center

Date Published: October 7, 2011  |  doi:10.3791/3140  |  Section: JoVE Immunology and Infection

Keywords: Immunology, herpesvirus, gamma herpesvirus 68, γHV68, signaling pathways, host-virus interaction, viral lytic replication

 JoVE Immunology and Infection

A Method for Labeling Vasculature in Embryonic Mice


JoVE 3267

1, 2, 3

1Department of Cellular Biology, University of Georgia, 2Centre for Immunology and Infection, Department of Biology and HYMS, University of York, 3Department of Genetics, University of Georgia

Date Published: October 7, 2011  |  doi:10.3791/3267  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Thymus, Skin, Endothelial Cell, Mesenchyme, Vasculature, CD31, FITC-dextran, isolectin B4, Facial Vein, Development, Labeling, Mice, Injection

 JoVE Immunology and Infection

Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy


JoVE 2950

1,2, 1, 1

1Institute of Plant Sciences, University of Graz, 2Institute for Electron Microscopy and Fine Structure Research, Graz University of Technology

Date Published: October 14, 2011  |  doi:10.3791/2950  |  Section: JoVE Immunology and Infection

Keywords: Immunology, diagnostics, electron microscopy, microwave, Nicotiana, negative staining, phytopathology, TMV, ultrastructure

 JoVE Immunology and Infection

Following Cell-fate in E. coli After Infection by Phage Lambda


JoVE 3363

1, 1,2,3

1Department of Physics, University of Illinois at Urbana-Champaign, 2Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, 3Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine

Date Published: October 14, 2011  |  doi:10.3791/3363  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Systems biology, Microbiology, fluorescently labeled bacteriophage lambda, E. coli, live-cell imaging

 JoVE Immunology and Infection

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres


JoVE 3344

1, 1, 2, 2, 3

1Saskatoon Research Centre, Agriculture and Agri-Food Canada, 2Department of Veterinary Microbiology, University of Saskatchewan, 3Plant Biotechnology Institute, National Research Council of Canada

Date Published: October 23, 2011  |  doi:10.3791/3344  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Medicine, chaperonin-60, hsp60, luminex, multiplex, diagnostics, bacterial vaginosis, PCR

 JoVE Clinical and Translational Medicine

Delivery of Therapeutic Agents Through Intracerebroventricular (ICV) and Intravenous (IV) Injection in Mice


JoVE 2968

1, 1, 2, 1, 3, 3

1Department of Molecular Microbiology and Immunology, Bond Life Sciences Center, University of Missouri, 2Department of Biological Sciences, Columbia University, 3Department of Veterinary Pathobiology, Bond Life Sciences Center, University of Missouri

Date Published: October 3, 2011  |  doi:10.3791/2968  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Neuroscience, Motor neuron, brain, CNS, temporal vein, mouse, injection, ventricles

 JoVE Clinical and Translational Medicine

In vivo Bioluminescence Imaging of Tumor Hypoxia Dynamics of Breast Cancer Brain Metastasis in a Mouse Model


JoVE 3175

1, 2, 2, 3, 3, 2, 2

1Department of Radiation Oncology, University of Texas Southwestern Medical Center, 2Department of Radiology, University of Texas Southwestern Medical Center, 3Department of Radiation Oncology, Kyoto University Graduate School of Medicine

Date Published: October 3, 2011  |  doi:10.3791/3175  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, bioluminescence imaging (BLI), tumor hypoxia dynamics, hypoxia inducible factor-1α (HIF-1α), breast cancer brain metastasis

 JoVE Clinical and Translational Medicine

Stem Cell Transplantation Strategies for the Restoration of Cognitive Dysfunction Caused by Cranial Radiotherapy


JoVE 3107

*, *, , *,

Department of Radiation Oncology, University of California Irvine

Date Published: October 18, 2011  |  doi:10.3791/3107  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, neuroscience, radiotherapy, cognitive dysfunction, stem cells, transplantation

 JoVE Clinical and Translational Medicine

Quantifying Cognitive Decrements Caused by Cranial Radiotherapy


JoVE 3108

, ,

Department of Radiation Oncology, University of California Irvine

Date Published: October 18, 2011  |  doi:10.3791/3108  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, neuroscience, radiotherapy, cognitive dysfunction, hippocampus, novel place recognition, elevated plus maze, fear conditioning, water maze

 JoVE Clinical and Translational Medicine

Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy


JoVE 2967

1,2, 1, 3, 3, 2, 4, 1

1Department of Orthodontics and Maxillofacial Orthopedics, Medical University of Graz, 2Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz, 3Department of Prosthodontics, Restorative Dentistry, Periodontology and Implantology, Medical University of Graz, 4Institute of Plant Sciences, Karl-Franzens-University Graz

Date Published: October 20, 2011  |  doi:10.3791/2967  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, fluorescence in situ hybridization, FISH, confocal laser scanning microscopy, CLSM, orthodontic appliances, oral biofilm

 JoVE Clinical and Translational Medicine

Experimental Generation of Carcinoma-Associated Fibroblasts (CAFs) from Human Mammary Fibroblasts


JoVE 3201

*1, *1, 1,2

1CR-UK Stromal-Tumour Interaction Group, Paterson Institute for Cancer Research, University of Manchester, 2Atopy Research Center, Juntendo University

Date Published: October 25, 2011  |  doi:10.3791/3201  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, cancer, stromal myofibroblasts, experimentally generated carcinoma-associated fibroblasts (exp-CAFs), fibroblast, human mammary carcinomas, tumour xenografts

 JoVE Clinical and Translational Medicine

Heterogeneity Mapping of Protein Expression in Tumors using Quantitative Immunofluorescence


JoVE 3334

1, 2, 2, 2, 2, 1, 1

1Division of Pathology, University of Edinburgh, 2HistoRx Inc.

Date Published: October 25, 2011  |  doi:10.3791/3334  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, quantitative immunofluorescence, heterogeneity, cancer, biomarker, targeted therapy, immunohistochemistry, proteomics, histopathology

 JoVE Bioengineering

Adaptation of a Haptic Robot in a 3T fMRI


JoVE 3364

1, 1, 2, 2, 3

1Institute for Neural Computation, University of California, 2Department of Radiology, University of California, 3Department of Cognitive Science and Program in Neurosciences, University of California

Date Published: October 4, 2011  |  doi:10.3791/3364  |  Section: JoVE Bioengineering

Keywords: Bioengineering, neuroscience, haptic robot, fMRI, MRI, pointing

 JoVE Bioengineering

Synthesis, Assembly, and Characterization of Monolayer Protected Gold Nanoparticle Films for Protein Monolayer Electrochemistry


JoVE 3441

1, 2, 2, 2, 1

1Department of Chemistry, Gottwald Center for the Sciences, University of Richmond, 2Department of Biochemistry and Molecular Biology, Gottwald Center for the Sciences, University of Richmond

Date Published: October 4, 2011  |  doi:10.3791/3441  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Monolayer protected clusters, film assemblies, protein monolayer electrochemistry, azurin, self-assembled monolayers

 JoVE Bioengineering

Organotypic Collagen I Assay: A Malleable Platform to Assess Cell Behaviour in a 3-Dimensional Context


JoVE 3089

1, 1, 1, 1, 2, 2, 1

1The Beatson Institute for Cancer Research, University of Glasgow, 2Section of Dermatology, School of Medicine, University of Glasgow

Date Published: October 13, 2011  |  doi:10.3791/3089  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Organotypic culture, cell migration, invasion, 3-dimensional matrix, Collagen I, second harmonic generation, host-tumor interaction, microenvironment

 JoVE Bioengineering

Video-rate Scanning Confocal Microscopy and Microendoscopy


JoVE 3252

1,2,3, 1,3

1Program in Biophysics, Harvard University, 2Division of Health Sciences and Technology, Harvard-MIT, 3Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School

Date Published: October 20, 2011  |  doi:10.3791/3252  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Microscopy, confocal microscopy, microendoscopy, video-rate, fluorescence, scanning, in vivo imaging

 JoVE Bioengineering

GENPLAT: an Automated Platform for Biomass Enzyme Discovery and Cocktail Optimization


JoVE 3314

1,2, 2, 2

1DOE Plant Research Laboratory, Michigan State University, 2DOE Great Lakes Bioenergy Research Center, Michigan State University

Date Published: October 24, 2011  |  doi:10.3791/3314  |  Section: JoVE Bioengineering

Keywords: Bioengineering, cellulase, cellobiohydrolase, glucanase, xylanase, hemicellulase, experimental design, biomass, bioenergy, corn stover, glycosyl hydrolase

 JoVE Bioengineering

Quantitatively Measuring In situ Flows using a Self-Contained Underwater Velocimetry Apparatus (SCUVA)


JoVE 2615

1, 2,3, 3,4, 5

1Applied Ocean Physics and Engineering, Woods Hole Oceanographic Institution, 2Environmental Science and Marine Biology, Roger Williams University, 3Marine Biology Laboratory, Whitman Center, 4Department of Biology, Providence College, 5Departments of Aeronautics and Bioengineering, California Institute of Technology

Date Published: October 31, 2011  |  doi:10.3791/2615  |  Section: JoVE Bioengineering

Keywords: Bioengineering, In situ DPIV, SCUVA, animal flow measurements, zooplankton, propulsion

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