Table of Contents: Issue 29, July 2009

The 2009 Lindau Nobel Laureate Meeting: Aaron Ciechanover, Chemistry 2004

JoVE 1559

Aaron Ciechanover

Date Published: July 1, 2009  |  doi:10.3791/1559  |  Section: JoVE Editorial

Keywords: Cellular Biology, Ubiquitin, Ubiquitination, Proteosome, Protein Degradation, Nobel Laureate,

An Interview with Nobel Laureate Douglas Osheroff, Physics 1996

JoVE 1540

Douglas Osheroff

Date Published: July 7, 2009  |  doi:10.3791/1540  |  Section: JoVE Editorial

Keywords: Physics, Nobel Laureate, Douglas Osheroff, Superfluidity, Helium-3, Superconductivity

An Interview with Nobel Laureate Theodor Hansch, Physics 2005

JoVE 1537

Date Published: July 13, 2009  |  doi:10.3791/1537  |  Section: JoVE Editorial

Keywords: Physics, Nobel Laureate, Laser-Based Spectroscopy, Frequency Comb, Atomic Clocks, Quantum Optics,

Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator

JoVE 1266

Melanie Jungblut, Karen Oeltze, Irene Zehnter, Doris Hasselmann, Andreas Bosio

Miltenyi Biotec,GmbH

Date Published: July 2, 2009  |  doi:10.3791/1266  |  Section: JoVE General

Keywords: Cell Biology, cell culture, cell dissociation, lung, mouse

Using the Horseshoe Crab, Limulus Polyphemus, in Vision Research

JoVE 1384

Jiahui S. Liu, Christopher L. Passaglia

Department of Biomedical Engineering, Boston University

Date Published: July 3, 2009  |  doi:10.3791/1384  |  Section: JoVE General

Keywords: Neuroscience, electroretinogram, intracellular recording, extracellular recording, retina

A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization

JoVE 1395

Bruce W. Draper¹, Cecilia B. Moens²

¹Molecular and Cellular Biology, University of California, Davis, ²Division of Basic Science, Fred Hutchinson Cancer Research Center - FHCRC

Date Published: July 6, 2009  |  doi:10.3791/1395  |  Section: JoVE General

Keywords: Developmental Biology, Zebrafish, sperm, cryopreservation, TILLING

Generation of Single-Cell Suspensions from Mouse Neural Tissue

JoVE 1267

Sandra Pennartz, Sandy Reiss, Rebecca Biloune, Doris Hasselmann, Andreas Bosio

Miltenyi Biotec,GmbH

Date Published: July 7, 2009  |  doi:10.3791/1267  |  Section: JoVE General

Keywords: Basic Protocol, Neuroscience, cell culture, cell dissociation, neuron, mouse

Live Imaging of Glial Cell Migration in the Drosophila Eye Imaginal Disc

JoVE 1155

Patrick Cafferty, Xiaojun Xie, Kristen Browne, Vanessa J. Auld

Department of Zoology, University of British Columbia - UBC

Date Published: July 9, 2009  |  doi:10.3791/1155  |  Section: JoVE General

Keywords: Neuroscience, Drosophila, migrating glial cells, optic stalk, imaginal disc, live imaging, ventral nerve cord, GFP-tagged glial cells, reverse polarity promoter, mouth hooks

Murine Renal Transplantation Procedure

JoVE 1150

Jiao-Jing Wang¹, Sara Hockenheimer², Alice A. Bickerstaff¹, Gregg A. Hadley¹

¹Department of Surgery, The Ohio State University, ²Integrated Biomedical Sciences Graduate Program, The Ohio State University

Date Published: July 10, 2009  |  doi:10.3791/1150  |  Section: JoVE General

Keywords: immunology, mouse, kidney, renal, transplantation, procedure

An In Vitro Skin Irritation Test (SIT) using the EpiDerm Reconstructed Human Epidermal (RHE) Model

JoVE 1366

Helena Kandárová, Patrick Hayden, Mitchell Klausner, Joseph Kubilus, John Sheasgreen

MatTek Corporation

Date Published: July 13, 2009  |  doi:10.3791/1366  |  Section: JoVE General

Keywords: Medicine, Skin Irritation, EpiDerm, REACH, In Vitro, RHE model, ECVAM, Validation, 3D tissue model

Bioelectric Analyses of an Osseointegrated Intelligent Implant Design System for Amputees

JoVE 1237

Brad M. Isaacson^1,2, Jeroen G. Stinstra³, Rob S. MacLeod^2,3, Joseph B. Webster^1,4, James P. Beck^1,5, Roy D. Bloebaum^1,2,5

¹Department of Veteran Affairs, ²Department of Bioengineering, University of Utah, ³Scientific Computing and Imaging Institute , University of Utah, ⁴Department of Physical Medicine and Rehabilitation, University of Utah, ⁵Department of Orthopaedics, University of Utah

Date Published: July 15, 2009  |  doi:10.3791/1237  |  Section: JoVE General

Keywords: Medicine, Osseointegration, Electrical Stimulation, Osteogenesis, Amputation, Percutaneous

Dissecting the Non-human Primate Brain in Stereotaxic Space

JoVE 1259

Mark W. Burke¹, Shahin Zangenehpour², Denis Boire³, Maurice Ptito²

¹Department of Physiology, University of Montreal, ²School of Optometry, University of Montreal, ³Département de chimie-biologie
, Université du Québes à Trois-Rivières

Date Published: July 16, 2009  |  doi:10.3791/1259  |  Section: JoVE General

Keywords: Neuroscience, Non-human primate, brain bank, stereotaxic apparatus, cryostat, dissection

Generating Chimeric Zebrafish Embryos by Transplantation

JoVE 1394

Hilary A. Kemp, Amanda Carmany-Rampey, Cecilia Moens

HHMI and Division of Basic Sciences, Fred Hutchinson Cancer Research Center - FHCRC

Date Published: July 17, 2009  |  doi:10.3791/1394  |  Section: JoVE General

Keywords: Developmental Biology, development, mosaic analysis, chimera, zebrafish embryo, gastrula, blastula, targeted, transplantation

An In vitro FluoroBlok Tumor Invasion Assay

JoVE 1475

Jeff Partridge, Paula Flaherty

BD Biosciences, Discovery Labware

Date Published: July 20, 2009  |  doi:10.3791/1475  |  Section: JoVE General

Keywords: Cellular Biology, Tumor Invasion Assay, Chemotaxis, Calcein-AM, Matrigel, Falcon, Fluoroblok, Migration, Invasion, Tumor, BD, Matrigel, Boyden chamber, Motility, Haptotaxis

Cell Block Preparation from Cytology Specimen with Predominance of Individually Scattered Cells

JoVE 1316

George M. Varsegi, Vinod Shidham

Department of Pathology, University of Wisconsin - Milwaukee

Date Published: July 21, 2009  |  doi:10.3791/1316  |  Section: JoVE General

Keywords: Cellular Biology, surgical pathology, cytopathology, FNA, cellblocks, SCIP. immunohistochemistry

Recordings of Neural Circuit Activation in Freely Behaving Animals

JoVE 1297

Jens Herberholz

Department of Psychology, Neuroscience and Cognitive Science Program , University of Maryland

Date Published: July 22, 2009  |  doi:10.3791/1297  |  Section: JoVE General

Keywords: Neuroscience, Electrophysiology, bath electrodes, neurons, behavior

Creation of Murine Experimental Abdominal Aortic Aneurysms with Elastase

JoVE 1280

Junya Azuma¹, Tomoko Asagami², Ronald Dalman², Philip S. Tsao¹

¹Department of Cardiovascular Medicine, Stanford University School of Medicine, ²Department of Vascular Surgery, Stanford University School of Medicine

Date Published: July 23, 2009  |  doi:10.3791/1280  |  Section: JoVE General

Keywords: Medicine, abdominal aortic aneurysm, AAA, mouse, elastase

Brain Banking: Making the Most of your Research Specimens

JoVE 1260

Mark W. Burke¹, Shahin Zangenehpour², Maurice Ptito²

¹Department of Physiology, University of Montreal, ²School of Optometry, University of Montreal

Date Published: July 24, 2009  |  doi:10.3791/1260  |  Section: JoVE General

Keywords: Neuroscience, brain bank, systematic sampling, stereology, cryostat, antigen preserve

Batch Immunostaining for Large-Scale Protein Detection in the Whole Monkey Brain

JoVE 1286

Shahin Zangenehpour^1,2, Mark W. Burke², Avi Chaudhuri³, Maurice Ptito²

¹Cognitive Neuroscience Unit, Montreal Neurological Institute, ²Ècole d’Optomètrie, Universitè de Montrèal, ³Department of Psychology, McGill University

Date Published: July 27, 2009  |  doi:10.3791/1286  |  Section: JoVE General

Keywords: Neuroscience, brain, immunohistochemistry, monkey, non-human primate, antibody, SMI32, FMRP, NeuN

Exploring Cognitive Functions in Babies, Children & Adults with Near Infrared Spectroscopy

JoVE 1268

Mark H. Shalinsky¹, Iouila Kovelman¹, Melody S. Berens², Laura-Ann Petitto²

¹Department of Psychology, University of Michigan, Ann Arbor, ²Department of Psychology, University of Toronto Scarborough

Date Published: July 28, 2009  |  doi:10.3791/1268  |  Section: JoVE General

Keywords: Neuroscience, infant, child, Near Infrared Spectroscopy, fNIRS, optical tomography, cognitive neuroscience, psychology, brain, developmental cognitive neuroscience, analysis

Using an EEG-Based Brain-Computer Interface for Virtual Cursor Movement with BCI2000

JoVE 1319

J. Adam Wilson¹, Gerwin Schalk², Léo M. Walton¹, Justin C. Williams¹

¹Department of Biomedical Engineering, University of Wisconsin-Madison, ²Wadsworth Center, New York State Dept. of Health

Date Published: July 29, 2009  |  doi:10.3791/1319  |  Section: JoVE General

Keywords: Neuroscience, BCI, EEG, brain-computer interface, BCI2000

Making MR Imaging Child's Play - Pediatric Neuroimaging Protocol, Guidelines and Procedure

JoVE 1309

Nora M. Raschle^1,2, Michelle Lee¹, Roman Buechler¹, Joanna A. Christodoulou³, Maria Chang¹, Monica Vakil¹, Patrice L. Stering¹, Nadine Gaab^1,3,4

¹Department of Developmental Medicine, Children’s Hospital Boston, ²Department of Neuropsychology, University of Zurich, ³Graduate School of Education, Harvard, ⁴Harvard Medical School

Date Published: July 30, 2009  |  doi:10.3791/1309  |  Section: JoVE General

Keywords: Neuroscience, fMRI, imaging, development, children, pediatric neuroimaging, cognitive development, magnetic resonance imaging, pediatric imaging protocol, patient preparation, mock scanner

Psychophysiological Stress Assessment Using Biofeedback

JoVE 1443

Inna Khazan^1,2

¹Behavioral Medicine, Cambridge Health Alliance, ²Harvard Medical School

Date Published: July 31, 2009  |  doi:10.3791/1443  |  Section: JoVE General

Keywords: Neuroscience, Stress, biofeedback, psychophysiological, assessment