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The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.

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Table of Contents

Table of Contents: Issue 57, November 2011

Jump to:  JoVE Editorial (1) JoVE General (14) JoVE Neuroscience (12) JoVE Immunology and Infection (8) JoVE Clinical and Translational Medicine (9) JoVE Bioengineering (7) JoVE Errata (1)
 JoVE General

A Protocol for Computer-Based Protein Structure and Function Prediction


JoVE 3259

1,2, 1, 1, 1,2

1Center for Computational Medicine and Bioinformatics, University of Michigan, 2Center for Bioinformatics and Department of Molecular Bioscience, University of Kansas

Date Published: November 3, 2011  |  doi:10.3791/3259  |  Section: JoVE General

Keywords: Biochemistry, On-line server, I-TASSER, protein structure prediction, function prediction

 JoVE General

Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction


JoVE 3274

1,2, 3,4, 1,2, 1,2,5, 1,2,6, 1,2

1San Diego Regenerative Medicine Institute, 2Xcelthera, 3Department of Neurosurgery, Harvard Medical School, 4Division of SCI Research, VA Boston Healthcare System, 5Program in Stem Cell & Regenerative Biology, Sanford-Burnham Medical Research Institute, 6La Jolla IVF

Date Published: November 3, 2011  |  doi:10.3791/3274  |  Section: JoVE General

Keywords: Developmental Biology, human embryonic stem cell, human, cardiac progenitor, cardiomyocytes, human pluripotent cell, cardiac differentiation, small molecule induction, cell culture, cell therapy

 JoVE General

Ice-Cap: A Method for Growing Arabidopsis and Tomato Plants in 96-well Plates for High-Throughput Genotyping


JoVE 3280

1, 2, 1, 1, 1

1Horticulture Department, University of Wisconsin-Madison, 2Department of Zoology, Oregon State University

Date Published: November 9, 2011  |  doi:10.3791/3280  |  Section: JoVE General

Keywords: Plant Biology, Plant, Arabidopsis thaliana, tomato, 96-well plate, DNA extraction, high-throughput, genotyping

 JoVE General

Assessment of GFP Expression and Viability Using the Tali Image-Based Cytometer


JoVE 3659

1, 2

1Department of Molecular and Cell Biology, Life Technologies, 2Life Technologies

Date Published: November 17, 2011  |  doi:10.3791/3659  |  Section: JoVE General

Keywords: Cell Biology, cytometry, imaging, image-based, cell viability, apoptosis, cell counting, expression assay, Dead-Cell Red, propidium iodide, PI, GFP expression, RFP expression, cell analysis, fluorescence protein expression

 JoVE General

Visualization of Mitochondrial Respiratory Function using Cytochrome C Oxidase / Succinate Dehydrogenase (COX/SDH) Double-labeling Histochemistry


JoVE 3266

1,2

1Department of Neuroscience, Karolinska Institutet, 2National Institute on Drug Abuse (NIDA)

Date Published: November 23, 2011  |  doi:10.3791/3266  |  Section: JoVE General

Keywords: Cellular Biology, aging, brain, COX/SDH, histochemistry, mitochondria, mitochondrial disease, mitochondrial dysfunction, mtDNA, mtDNA mutations, respiratory chain

 JoVE General

A Quantitative Assay for Insulin-expressing Colony-forming Progenitors


JoVE 3148

1, 2, 2, 2, 2, 2, 2,3

1Department of Biotechnology & Bioinformatics, California State University Channel Islands, 2Department of Diabetes, Endocrinology and Metabolism, Beckman Research Institute of City of Hope, 3The Irell & Manella Graduate School of Biological Sciences, Beckman Research Institute of City of Hope

Date Published: November 28, 2011  |  doi:10.3791/3148  |  Section: JoVE General

Keywords: Developmental Biology, Pancreas, insulin-expressing cells, embryonic stem cells, colony assay, progenitor cells, 3-dimensional culture, semi-solid media, Matrigel, methylcellulose

 JoVE General

Imaging of Estrogen Receptor-α in Rat Pial Arterioles using a Digital Immunofluorescent Microscope


JoVE 3203

, , ,

Graduate School of Nursing, Uniformed Services University of the Health Sciences

Date Published: November 29, 2011  |  doi:10.3791/3203  |  Section: JoVE General

Keywords: Molecular Biology, digital immunofluorescent microscopy, brain, estrogen receptor-α, cerebral microvasculature, rat, immunohistochemistry

 JoVE Neuroscience

Morphological Analysis of Drosophila Larval Peripheral Sensory Neuron Dendrites and Axons Using Genetic Mosaics


JoVE 3111

1,2, 1

1Disease Mechanism Research Core, RIKEN Brain Science Institute, 2Graduate School of Science and Engineering, Saitama University

Date Published: November 7, 2011  |  doi:10.3791/3111  |  Section: JoVE Neuroscience

Keywords: Neuroscience, developmental biology, sensory neuron, Drosophila, larvae, immunohistochemistry, dendritic arborization neurons, peripheral nervous system, MARCM, Flp-out

 JoVE Neuroscience

Immunohistological Labeling of Microtubules in Sensory Neuron Dendrites, Tracheae, and Muscles in the Drosophila Larva Body Wall


JoVE 3662

1,2, 1,2, 1

1Disease Mechanism Research Core, RIKEN Brain Science Institute, 2Graduate School of Science and Engineering, Saitama University

Date Published: November 10, 2011  |  doi:10.3791/3662  |  Section: JoVE Neuroscience

Keywords: Neuroscience, developmental biology, Drosophila larvae, immunohistochemistry, microtubule, trachea, dendritic arborization neurons

 JoVE Neuroscience

Quantitative Analysis of Synaptic Vesicle Pool Replenishment in Cultured Cerebellar Granule Neurons using FM Dyes


JoVE 3143

,

Membrane Biology Group, Centre for integrative Physiology, University of Edinburgh

Date Published: November 11, 2011  |  doi:10.3791/3143  |  Section: JoVE Neuroscience

Keywords: Neuroscience, synaptic vesicle, neuron, recycling pool, readily releasable pool, reserve pool, replenishment, FM dyes, exocytosis, endocytosis

 JoVE Neuroscience

Behavioral Assessment of Manual Dexterity in Non-Human Primates


JoVE 3258

*, *, , , , , , , , ,

Department of Medicine, University of Fribourg

Date Published: November 11, 2011  |  doi:10.3791/3258  |  Section: JoVE Neuroscience

Keywords: Neuroscience, monkey, hand, spinal cord lesion, cerebral cortex lesion, functional recovery

 JoVE Neuroscience

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling


JoVE 3125

, , ,

Center for Sleep and Circadian Biology, Northwestern University

Date Published: November 13, 2011  |  doi:10.3791/3125  |  Section: JoVE Neuroscience

Keywords: Neuroscience, transcriptome, RNA-Seq, microdissection, total RNA, brain, mouse, microarray, RNA, RT-qPCR, gene, expression

 JoVE Neuroscience

Cerebrovascular Casting of the Adult Mouse for 3D Imaging and Morphological Analysis


JoVE 2958

1, 1, 1,2,3, 1

1Center for Cerebrovascular Research, Department of Anesthesia and Perioperative Care, University of California, San Francisco, 2Department of Neurological Surgery, University of California, San Francisco, 3Department of Neurology, University of California, San Francisco

Date Published: November 30, 2011  |  doi:10.3791/2958  |  Section: JoVE Neuroscience

Keywords: Neuroscience, vessel, vascular cast, capillary, cerebrovasculature, brain, blood, AVM, fistula

 JoVE Immunology and Infection

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy


JoVE 3400

, , ,

Department of Pathology, University of Texas Medical Branch

Date Published: November 1, 2011  |  doi:10.3791/3400  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Rift Valley fever virus, reverse genetics, NSs, MP-12, vaccine development

 JoVE Immunology and Infection

Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines


JoVE 3321

1,2, 1,2, 2, 2, 1,2,3,4

1Stony Brook Children's Hospital, State University of New York at Stony Brook, 2Department of Pediatrics, State University of New York at Stony Brook, 3Department of Molecular Genetics, State University of New York at Stony Brook, 4Department of Microbiology, State University of New York at Stony Brook

Date Published: November 8, 2011  |  doi:10.3791/3321  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Epstein-Barr virus, EBV, lymphoblastoid cell lines, LCL, transformation, immortalization, PBMC

 JoVE Immunology and Infection

Measurement of γHV68 Infection in Mice


JoVE 3472

, , , , ,

Department of Molecular Microbiology and Immunology, University of Southern California, Los Angeles

Date Published: November 22, 2011  |  doi:10.3791/3472  |  Section: JoVE Immunology and Infection

Keywords: Immunology, γHV68, herpesvirus, viral infection, plaque assay, infectious center assay, PCR, qPCR, host-virus interaction

 JoVE Immunology and Infection

Production and Titering of Recombinant Adeno-associated Viral Vectors


JoVE 3348

*1, *1, 1, 2, 1,3

1School of Medical Sciences, College of Life Sciences and Medicine, University of Aberdeen, 2Translational Neuroscience Facility and Department of Physiology, School of Medical Sciences, University of New South Wales, 3Department of Biochemistry and Molecular Biophysics, Columbia University

Date Published: November 27, 2011  |  doi:10.3791/3348  |  Section: JoVE Immunology and Infection

Keywords: Immunology, adeno-associated virus, AAV, virus titer, stereotaxic injection, viral gene transfer

 JoVE Immunology and Infection

Determining the Phagocytic Activity of Clinical Antibody Samples


JoVE 3588

1, 1, 1, 1, 1, 2

1Massachusetts General Hospital, Ragon Institute of MGH, MIT, and Harvard, 2Thayer School of Engineering, Dartmouth College

Date Published: November 30, 2011  |  doi:10.3791/3588  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Phagocytosis, Antibody, ADCC, Effector Function, Fc receptor, antibody-dependent phagocytosis, monocytes

 JoVE Clinical and Translational Medicine

Labeling Stem Cells with Ferumoxytol, an FDA-Approved Iron Oxide Nanoparticle


JoVE 3482

1,2, 1,2, 1,2, 1

1Department of Radiology, Molecular Imaging Program at Stanford (MIPS), 2Stanford School of Medicine, Stanford University

Date Published: November 4, 2011  |  doi:10.3791/3482  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, USPIO, cell labeling, MR imaging, MRI, molecular imaging, iron oxides, ferumoxytol, cellular imaging, nanoparticles

 JoVE Clinical and Translational Medicine

Stem Cell Transplantation in an in vitro Simulated Ischemia/Reperfusion Model


JoVE 3575

, , , ,

Institute of Human Physiology and Clinical Experimental Research, Semmelweis University

Date Published: November 5, 2011  |  doi:10.3791/3575  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, ischemia/reperfusion model, stem cell transplantation, confocal microscopy, flow cytometry

 JoVE Clinical and Translational Medicine

Intracranial Implantation with Subsequent 3D In Vivo Bioluminescent Imaging of Murine Gliomas


JoVE 3403

1, 2, 2, 1,2

1Neuro-Oncology Research, Barrow Neurological Institute of St. Joseph’s Hospital and Medical Center, 2Neurosurgery Research Laboratory, Barrow Neurological Institute of St. Joseph’s Hospital and Medical Center

Date Published: November 6, 2011  |  doi:10.3791/3403  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, glioma, mouse model, bioluminescence, in vivo imaging, intracranial implantation

 JoVE Clinical and Translational Medicine

Teratoma Generation in the Testis Capsule


JoVE 3177

1, 1, 1, 1, 1, 2, 3, 1

1Department of Chemical Physiology, Scripps Research Institute, 2Department of Chemical Physiology, Scripps Research Institute, 3Department of Reproductive Medicine, University of California

Date Published: November 7, 2011  |  doi:10.3791/3177  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, stem cells, pluripotent stem cells, hPSCs, teratoma assay, animal model, mouse testis capsule

 JoVE Clinical and Translational Medicine

Hyperinsulinemic-euglycemic Clamps in Conscious, Unrestrained Mice


JoVE 3188

1, 2,3, 3, 2,3, 3, 4, 2,3, 2,3

1Diabetes and Obesity Research Center, Sanford-Burnham Medical Research Institute at Lake Nona, 2Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, 3Vanderbilt Mouse Metabolic Phenotyping Center, Vanderbilt University School of Medicine, 4Department of Pediatrics and Cellular and Integrative Physiology, Indiana University School of Medicine

Date Published: November 16, 2011  |  doi:10.3791/3188  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Glucose, insulin, clamp, mice, insulin resistance, diabetes, liver, muscle, conscious, restraint-free, non-stressed

 JoVE Clinical and Translational Medicine

Mouse Eye Enucleation for Remote High-throughput Phenotyping


JoVE 3184

1,2, 1,2, 2,3, 1,2, 2,4

1Department of Ophthalmology and Visual Sciences, University of Iowa, 2Omics Laboratory, University of Iowa, 3School of Dentistry, UCLA, 4Bernard and Shirlee Brown Glaucoma Laboratory, Department of Ophthalmology, College of Physicians and Surgeons, Columbia University

Date Published: November 19, 2011  |  doi:10.3791/3184  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, mouse, transgenic, phenomics, ophthalmology, retina, high-throughput, phenotyping

 JoVE Clinical and Translational Medicine

Implantation of Radiotelemetry Transmitters Yielding Data on ECG, Heart Rate, Core Body Temperature and Activity in Free-moving Laboratory Mice


JoVE 3260

1, 2, 2, 1

1Division of Surgical Research, University Hospital Zurich, 2Institute of Laboratory Animal Science, University of Zurich

Date Published: November 21, 2011  |  doi:10.3791/3260  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, telemetry, mouse, mice, transmitter implantation, humane endpoint, post-operative care, intensive care, recovery, surgery

 JoVE Bioengineering

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis


JoVE 3399

1,2, 1,2, 1,2

1Centers for Cancer Pharmacology and Excellence in Environmental Toxicology, University of Pennsylvania, 2Department of Pharmacology, University of Pennsylvania

Date Published: November 17, 2011  |  doi:10.3791/3399  |  Section: JoVE Bioengineering

Keywords: Bioengineering, lipids, extraction, stable isotope dilution, chiral chromatography, electron capture, mass spectrometry

 JoVE Bioengineering

Directed Cellular Self-Assembly to Fabricate Cell-Derived Tissue Rings for Biomechanical Analysis and Tissue Engineering


JoVE 3366

, , ,

Biomedical Engineering Department, Worcester Polytechnic Institute

Date Published: November 25, 2011  |  doi:10.3791/3366  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Cell-derived matrix, vascular tissue engineering, smooth muscle cells, cellular self-assembly, tissue biomechanics

 JoVE Bioengineering

Detection and Isolation of Circulating Melanoma Cells using Photoacoustic Flowmetry


JoVE 3559

, , , , , ,

Biological Engineering, Christopher S. Bond Life Sciences Center, Dermatology, University of Missouri

Date Published: November 25, 2011  |  doi:10.3791/3559  |  Section: JoVE Bioengineering

Keywords: Bioengineering, cancer, circulating tumor cell, CTCs, melanoma, metastasis, optoacoustic

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