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The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.

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Table of Contents

Table of Contents: Issue 72, February 2013

Jump to:  JoVE Editorial (1) JoVE General (11) JoVE Neuroscience (6) JoVE Immunology and Infection (6) JoVE Clinical and Translational Medicine (13) JoVE Bioengineering (3) JoVE Applied Physics (3) JoVE Chemistry (4)
 JoVE General

Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization


JoVE 50087

1, 1,2,3,4, 1

1Department of Pathology, New York University School of Medicine, 2New York University Center for Health Informatics and Bioinformatics, 3NYU Cancer Institute, 4Department of Pathology and Yale Cancer Center, Yale University School of Medicine

Date Published: February 3, 2013  |  doi:10.3791/50087  |  Section: JoVE General

Keywords: Genetics, Molecular Biology, Bioinformatics, Cancer Biology, Pathology, Biomedical Engineering, Immunology, Intranuclear Space, Nuclear Matrix, Fluorescence in situ Hybridization, FISH, 3D DNA FISH, DNA, immunofluorescence, immuno-FISH, 3D microscopy, Nuclear organization, interphase nuclei, chromatin modifications

 JoVE General

Evaluation of Muscle Function of the Extensor Digitorum Longus Muscle Ex vivo and Tibialis Anterior Muscle In situ in Mice


JoVE 50183

, ,

Department of Molecular Microbiology and Immunology, School of Medicine, University of Missouri

Date Published: February 9, 2013  |  doi:10.3791/50183  |  Section: JoVE General

Keywords: Medicine, Immunology, Microbiology, Anatomy, Physiology, Molecular Biology, Muscle, Skeletal, Neuromuscular Diseases, Drug Therapy, Gene Therapy, Musculoskeletal Diseases, Skeletal Muscle, Tibialis Anterior, Contractile Properties, Passive Properties, EDL, TA, animal model

 JoVE General

Using RNA-mediated Interference Feeding Strategy to Screen for Genes Involved in Body Size Regulation in the Nematode C. elegans


JoVE 4373

1, 2,3, 2,3

1Department of Science, Borough of Manhattan Community College, City Universtiy of New York (CUNY), 2Department of Biology, Queens College, The City University of New York (CUNY), 3Biochemistry Program, The Graduate Center, Queens College, The City University of New York (CUNY)

Date Published: February 13, 2013  |  doi:10.3791/4373  |  Section: JoVE General

Keywords: Developmental Biology, Genetics, Cellular Biology, Molecular Biology, Biochemistry, Basic Protocols, RNAi feeding technique, genetic screen, TGF-beta, body size, C. elegans, Caenorhabditis elegans, RNA-mediated Interference, RNAi, RNA, DNA, gene expression knock down, animal model

 JoVE General

RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells


JoVE 4393

, , , , , ,

Children's Mercy Hospital and Clinics, School of Medicine, University of Missouri-Kansas City

Date Published: February 13, 2013  |  doi:10.3791/4393  |  Section: JoVE General

Keywords: Genetics, Molecular Biology, Immunology, Medicine, Genomics, Proteins, RNA-seq, Next Generation DNA Sequencing, Transcriptome, Transcription, Thrombin, Endothelial cells, high-throughput, DNA, genomic DNA, RT-PCR, PCR

 JoVE General

Measuring Cation Transport by Na,K- and H,K-ATPase in Xenopus Oocytes by Atomic Absorption Spectrophotometry: An Alternative to Radioisotope Assays


JoVE 50201

1,2, 1, 1, 1

1Institute of Chemistry, Technical University of Berlin, 2The Vollum Institute, Oregon Health & Science University

Date Published: February 19, 2013  |  doi:10.3791/50201  |  Section: JoVE General

Keywords: Biochemistry, Chemistry, Biophysics, Bioengineering, Physiology, Molecular Biology, electrochemical processes, physical chemistry, spectrophotometry (application), spectroscopic chemical analysis (application), life sciences, temperature effects (biological, animal and plant), Life Sciences (General), Na+, K+-ATPase, H+, K+-ATPase, Cation Uptake, P-type ATPases, Atomic Absorption Spectrophotometry (AAS), Two-Electrode Voltage-Clamp, Xenopus Oocytes, Rb+ Flux, Transversely Heated Graphite Atomizer (THGA) Furnace, electrophysiology, animal model

 JoVE General

Microgavage of Zebrafish Larvae


JoVE 4434

,

Department of Cell and Molecular Physiology, University of North Carolina at Chapel Hill

Date Published: February 20, 2013  |  doi:10.3791/4434  |  Section: JoVE General

Keywords: Developmental Biology, Molecular Biology, Anatomy, Physiology, Basic Protocols, Surgery, Zebrafish, Danio rerio, intestine, lumen, larvae, gavage, microgavage, epithelium, barrier function, gut motility, microsurgery, microscopy, animal model

 JoVE General

Design and Use of Multiplexed Chemostat Arrays


JoVE 50262

, , ,

Department of Genome Sciences, University of Washington

Date Published: February 23, 2013  |  doi:10.3791/50262  |  Section: JoVE General

Keywords: Genetics, Molecular Biology, Microbiology, Biochemistry, Cellular Biology, Basic Protocols, Genomics, Eukaryota, Bacteria, Biological Phenomena, Metabolic Phenomena, Genetic Phenomena, Microbiological Phenomena, Life sciences, chemostat, evolution, experimental evolution, Ministat, yeast, E. coli., Physiology, Continuous culture, high throughput, arrays, cell culture

 JoVE General

Serial Enrichment of Spermatogonial Stem and Progenitor Cells (SSCs) in Culture for Derivation of Long-term Adult Mouse SSC Lines


JoVE 50017

,

Department of Surgery, Weill Cornell Medical College

Date Published: February 25, 2013  |  doi:10.3791/50017  |  Section: JoVE General

Keywords: Stem Cell Biology, Molecular Biology, Cellular Biology, Medicine, Genetics, Developmental Biology, Anatomy, Surgery, Spermatogonial Stem cells, Stem cells, feeder cells, germ cells, testis, cell culture, microenvironment, stem cell niche, progenitor cells, mice, transgenic mice, animal model

 JoVE General

Trajectory Data Analyses for Pedestrian Space-time Activity Study


JoVE 50130

1, 2

1School of Environmental and Life Sciences, Kean University, 2Department of Geography, University of Wisconsin-Madison

Date Published: February 25, 2013  |  doi:10.3791/50130  |  Section: JoVE General

Keywords: Environmental Sciences, Computer Science, Behavior, Infectious Diseases, Geography, Cartography, Data Display, Disease Outbreaks, cartography, human behavior, Trajectory data, space-time activity, GPS, GIS, ArcGIS, spatiotemporal analysis, visualization, segmentation, density surface, density volume, exploratory data analysis, modelling

 JoVE General

Rapid Genetic Analysis of Epithelial-Mesenchymal Signaling During Hair Regeneration


JoVE 4344

*, *, ,

Program in Epithelial Biology, Stanford University School of Medicine

Date Published: February 28, 2013  |  doi:10.3791/4344  |  Section: JoVE General

Keywords: Genetics, Tissue Engineering, Medicine, Biomedical Engineering, Cellular Biology, Surgery, Epithelial Biology, regeneration, chamber, hair, follicle, dermis, dermal cells, keratinocyte, graft, epithelial, cell culture, lentivirus, knockdown, shRNA-mediated knockdown, overexpression, mice, transgenic mice, animal model

 JoVE Neuroscience

Intact Histological Characterization of Brain-implanted Microdevices and Surrounding Tissue


JoVE 50126

1, 1, 2, 1, 1,2

1Weldon School of Biomedical Engineering, Purdue University, 2Department of Biological Sciences, Purdue University

Date Published: February 11, 2013  |  doi:10.3791/50126  |  Section: JoVE Neuroscience

Keywords: Neurobiology, Neuroscience, Biomedical Engineering, Medicine, Central Nervous System, Brain, Neuroglia, Neurons, Immunohistochemistry (IHC), Histocytological Preparation Techniques, Microscopy, Confocal, nondestructive testing, bioengineering (man-machine systems), bionics, histology, brain implants, microelectrode arrays, immunohistochemistry, neuroprosthetics, brain machine interface, microscopy, thick tissue, optical clearing, animal model

 JoVE Neuroscience

Neonatal Subventricular Zone Electroporation


JoVE 50197

, ,

Department of Neurosurgery and Cellular & Molecular Physiology, Yale University School of Medicine

Date Published: February 11, 2013  |  doi:10.3791/50197  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Developmental Biology, Neurobiology, Molecular Biology, Cellular Biology, Physiology, Anatomy, Biomedical Engineering, Stem Cell Biology, Genetics, Neurogenesis, Growth and Development, Surgery, Subventricular Zone, Electroporation, Neural Stem Cells, NSC, subventricular zone, brain, DNA, injection, genetic engineering, neonatal pups, animal model

 JoVE Neuroscience

Vertical T-maze Choice Assay for Arthropod Response to Odorants


JoVE 50229

,

Entomology and Nematology Department, University of Florida

Date Published: February 14, 2013  |  doi:10.3791/50229  |  Section: JoVE Neuroscience

Keywords: Biochemistry, Molecular Biology, Basic Protocols, Entomology, Behavior, Eukaryota, Organic Chemicals, Chemical Actions and Uses, Life Sciences (General), Behavioral Sciences, Arthropod behavior, chemical ecology, olfactometer, chemotaxis, olfaction, attraction, repulsion, odorant, T-maze, psyllid, Diaphorina citri, insect, anthropod, insect model

 JoVE Neuroscience

Appetitive Associative Olfactory Learning in Drosophila Larvae


JoVE 4334

1, 1, 1,2, 1, 1,2

1Department of Biology, University of Konstanz, 2Department of Biology, University of Fribourg

Date Published: February 18, 2013  |  doi:10.3791/4334  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Developmental Biology, Neurobiology, Biochemistry, Molecular Biology, Physiology, Behavior, Drosophila, fruit fly, larvae, instar, olfaction, olfactory system, odor, 1-octanol, OCT, learning, reward, sugar, feeding, animal model

 JoVE Neuroscience

Detection of Protein Palmitoylation in Cultured Hippocampal Neurons by Immunoprecipitation and Acyl-Biotin Exchange (ABE)


JoVE 50031

,

Department of Cellular and Physiological Sciences, Brain Research Centre, University of British Columbia

Date Published: February 18, 2013  |  doi:10.3791/50031  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Biochemistry, Neurobiology, Molecular Biology, Cellular Biology, Physiology, Proteins, synapse, cultured hippocampal neurons, palmitoylation, lipid, immunoprecipitation, western blotting, biotin, Acyl-Biotin Exchange, ABE, neuron, brain, cell culture, rat, mouse, animal model

 JoVE Neuroscience

Identification of Olfactory Volatiles using Gas Chromatography-Multi-unit Recordings (GCMR) in the Insect Antennal Lobe


JoVE 4381

, ,

Department of Biology, University of Washington

Date Published: February 24, 2013  |  doi:10.3791/4381  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Neurobiology, Physiology, Biochemistry, Chemistry, Entomlogy, Behavior, electrophysiology, olfaction, olfactory system, insect, multi-channel recording, gas chromatography, pollination, bees, Bombus impatiens, antennae, brain, animal model

 JoVE Immunology and Infection

Clinical Application of Sleeping Beauty and Artificial Antigen Presenting Cells to Genetically Modify T Cells from Peripheral and Umbilical Cord Blood


JoVE 50070

1, 1, 1, 1, 2, 2, 2, 1, 1

1Division of Pediatrics, U.T. MD Anderson Cancer Center, 2Department of Stem Cell Transplantation and Cellular Therapy, U.T. MD Anderson Cancer Center

Date Published: February 1, 2013  |  doi:10.3791/50070  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Cellular Biology, Medicine, Molecular Biology, Cancer Biology, Biomedical Engineering, Hematology, Biochemistry, Genetics, T-Lymphocytes, Antigen-Presenting Cells, Leukemia, Lymphoid, Lymphoma, Antigens, CD19, Immunotherapy, Adoptive, Electroporation, Genetic Engineering, Gene Therapy, Sleeping Beauty, CD19, T cells, Chimeric Antigen Receptor, Artificial Antigen Presenting Cells, Clinical Trial, Peripheral Blood, Umbilical Cord Blood, Cryopreservation, Electroporation

 JoVE Immunology and Infection

Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells


JoVE 50058

1, 2, 1, 2, 1

1Department of Chemical and Biomolecular Engineering, University of Houston, 2Division of Pediatrics, Research Unit 907, University of Texas MD Anderson Cancer Center

Date Published: February 2, 2013  |  doi:10.3791/50058  |  Section: JoVE Immunology and Infection

Keywords: Cancer Biology, Immunology, Cellular Biology, Molecular Biology, Medicine, Chemical Engineering, Biomolecular Engineering, Bioengineering, Immunotherapy, Adoptive, Microfluidics, Nanowell arrays, PDMS, BioStation, T Cells, tumor target cells, labeling, cytotoxicity, microscopy, assay

 JoVE Immunology and Infection

Staphylococcus aureus Growth using Human Hemoglobin as an Iron Source


JoVE 50072

, ,

Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical School

Date Published: February 7, 2013  |  doi:10.3791/50072  |  Section: JoVE Immunology and Infection

Keywords: Infection, Immunology, Microbiology, Infectious Diseases, Cellular Biology, Pathology, Micronutrients, Bacterial Infections, Gram-Positive Bacterial Infections, Bacteriology, Staphylococcus aureus, iron acquisition, hemoglobin, bacterial growth, bacteria

 JoVE Immunology and Infection

Establishing a Liquid-covered Culture of Polarized Human Airway Epithelial Calu-3 Cells to Study Host Cell Response to Respiratory Pathogens In vitro


JoVE 50157

,

National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention (CDC)

Date Published: February 7, 2013  |  doi:10.3791/50157  |  Section: JoVE Immunology and Infection

Keywords: Infection, Immunology, Infectious Diseases, Medicine, Microbiology, Virology, Cellular Biology, Molecular Biology, Pathology, Respiratory Syncytial Viruses, Respiratory Syncytial Virus, Human, Cell Polarity, life sciences, Calu-3, polarized cell culture, epithelial cells, respiratory virus, liquid covered culture, virus, cell culture

 JoVE Immunology and Infection

Analysis of the Solvent Accessibility of Cysteine Residues on Maize rayado fino virus Virus-like Particles Produced in Nicotiana benthamiana Plants and Cross-linking of Peptides to VLPs


JoVE 50084

1,2, 1,2

1Plant Sciences Institute, Agricultural Research Service, United States Department of Agriculture, 2Molecular Plant Pathology Laboratory, Agricultural Research Service, United States Department of Agriculture

Date Published: February 14, 2013  |  doi:10.3791/50084  |  Section: JoVE Immunology and Infection

Keywords: Virology, Plant Biology, Infection, Molecular Biology, Biochemistry, Proteins, Chemicals and Drugs, Analytical, Diagnostic and Therapeutic Techniques and Equipment, Technology, Industry, Agriculture, Chemistry and materials, Virus-like particles (VLPs), VLP, sulfhydryl-reactive chemistries, labeling, cross-linking, multivalent display, Maize rayado fino virus, mosaic virus, virus, nanoparticle, drug delivery, peptides, Nicotiana benthamiana, plant model

 JoVE Immunology and Infection

The Citrobacter rodentium Mouse Model: Studying Pathogen and Host Contributions to Infectious Colitis


JoVE 50222

, , , , ,

Division of Gastroenterology, BC Children's Hospital

Date Published: February 19, 2013  |  doi:10.3791/50222  |  Section: JoVE Immunology and Infection

Keywords: Infection, Immunology, Medicine, Infectious Diseases, Anatomy, Physiology, Biomedical Engineering, Microbiology, Gastrointestinal Tract, Gram-Negative Bacterial Infections, Colitis, Inflammatory Bowel Diseases, Infectious colitis, Inflammatory Bowel Disease, colitis, hyperplasia, immunostaining, epithelial barrier integrity, FITC-dextran, oral gavage, mouse, animal model

 JoVE Clinical and Translational Medicine

Identification of Sleeping Beauty Transposon Insertions in Solid Tumors using Linker-mediated PCR


JoVE 50156

1,2, 1,2

1Department of Obstetrics, Gynecology & Women's Health, Masonic Cancer Center, University of Minnesota, Minneapolis, 2Department of Genetics, Cell Biology & Development, Center for Genome Engineering, University of Minnesota, Minneapolis

Date Published: February 1, 2013  |  doi:10.3791/50156  |  Section: JoVE Clinical and Translational Medicine

Keywords: Genetics, Medicine, Cancer Biology, Biomedical Engineering, Genomics, Mice, Genetic Techniques, life sciences, animal models, Neoplasms, Genetic Phenomena, Forward genetic screen, cancer drivers, mouse models, oncogenes, tumor suppressor genes, Sleeping Beauty transposons, insertions, DNA, PCR, animal model

 JoVE Clinical and Translational Medicine

Time-lapse Imaging of Primary Preneoplastic Mammary Epithelial Cells Derived from Genetically Engineered Mouse Models of Breast Cancer


JoVE 50198

1, 1, 1, 1,2, 1,2, 3, 3, 1,2,4,5

1Department of Oncology, Georgetown University, 2Lombardi Comprehensive Cancer Center, Georgetown University, 3Stem Cell Dynamics, Helmholtz Zentrum München - German Research Center for Environmental Health, 4Department of Medicine, Georgetown University, 5Department of Nanobiomedical Science and WCU Research Center of Nanobiomedical Science, Dankook University

Date Published: February 8, 2013  |  doi:10.3791/50198  |  Section: JoVE Clinical and Translational Medicine

Keywords: Cancer Biology, Medicine, Cellular Biology, Molecular Biology, Anatomy, Physiology, Oncology, Mammary Glands, Animal, Epithelial Cells, Mice, Genetically Modified, Primary Cell Culture, Time-Lapse Imaging, Early Detection of Cancer, Models, Genetic, primary cell culture, preneoplastic mammary epithelial cells, genetically engineered mice, time-lapse imaging, BRCA1, animal model

 JoVE Clinical and Translational Medicine

Angiogenesis in the Ischemic Rat Lung


JoVE 50217

,

Johns Hopkins Asthma and Allergy Center, Division of Pulmonary and Critical Care Medicine, Johns Hopkins University

Date Published: February 8, 2013  |  doi:10.3791/50217  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Anatomy, Physiology, Biomedical Engineering, Pathology, Surgery, Lung, Lung Diseases, Lung Injury, Thoracic Surgical Procedures, Physiological Processes, Growth and Development, Respiratory System, Physiological Phenomena, angiogenesis, bronchial artery, blood vessels, arteries, rat, ischemia, intubation, artery ligation, thoracotomy, cannulation, animal model

 JoVE Clinical and Translational Medicine

Cardiac Stress Test Induced by Dobutamine and Monitored by Cardiac Catheterization in Mice


JoVE 50050

, ,

Instituto de Ciencias, Facultad de Medicina, Clínica Alemana Universidad del Desarrollo

Date Published: February 10, 2013  |  doi:10.3791/50050  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Anatomy, Physiology, Cardiology, Surgery, Cardiovascular System, Cardiovascular Diseases, Life Sciences (General), Computer Programming and Software, cardiac stress test, dobutamine, cardiac catheterization, hemodynamic parameters, mice, animal model

 JoVE Clinical and Translational Medicine

Murine Fetal Echocardiography


JoVE 4416

Department of Medicine, Section of Cardiology, Institute for Cardiovascular Research, University of Chicago

Date Published: February 15, 2013  |  doi:10.3791/4416  |  Section: JoVE Clinical and Translational Medicine

Keywords: Biomedical Engineering, Medicine, Molecular Biology, Anatomy, Physiology, Cardiology, echocardiography, echocardiograph, cardiac development, pulse Doppler, non-invasive imaging, ultrasound, cardiovascular disease, cardiac structure, imaging, transgenic mice, mouse, animal model

 JoVE Clinical and Translational Medicine

Experimental Human Pneumococcal Carriage


JoVE 50115

1, 1,2,3, 1,2,4, 1, 5, 6, 1, 1

1Respiratory Infection Group, Liverpool School of Tropical Medicine, 2Royal Liverpool and Broadgreen, University Hospital Trust, 3Comprehensive Local Research Network, 4NIHR Biomedical Research Centre in Microbial Diseases, Royal Liverpool and Broadgreen University Hospitals NHS Trust, 5Institute of Lung Health, Respiratory Biomedical Unit, University Hospitals of Leicester NHS Trust & University of Leicester, 6Department of Clinical Infection Microbiology & Immunology, Institute of Infection & Global Health, University of Liverpool

Date Published: February 15, 2013  |  doi:10.3791/50115  |  Section: JoVE Clinical and Translational Medicine

Keywords: Infection, Medicine, Immmunology, Microbiology, Infectious Diseases, Anatomy, Physiology, Biomedical Engineering, Streptococcus pneumoniae, carriage, nasal wash, inoculation, human, vaccine studies, pneumonia, volunteer selection, clinical

 JoVE Clinical and Translational Medicine

Endothelin-1 Induced Middle Cerebral Artery Occlusion Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Rat


JoVE 50014

1, 2, 1, 3, 4, 4, 1, 3

1Department of Neurosurgery, University of Florida, 2Department of Anatomical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran, 3Department of Physiology and Functional Genomics, University of Florida, 4Department of Neurology, University of Florida

Date Published: February 16, 2013  |  doi:10.3791/50014  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Neuroscience, Neurobiology, Biomedical Engineering, Surgery, Anatomy, Physiology, Nervous System Diseases, Ischemic stroke, Endothelin-1, Cerebrovascular, brain, artery, stroke, occlusion, laser, doppley, flowmetry, rat, animal model

 JoVE Clinical and Translational Medicine

Optimized System for Cerebral Perfusion Monitoring in the Rat Stroke Model of Intraluminal Middle Cerebral Artery Occlusion


JoVE 50214

, , , , , , , , , ,

Department of Neuroscience and Biomedical Technologies, University of Milano Bicocca

Date Published: February 17, 2013  |  doi:10.3791/50214  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Neuroscience, Neurobiology, Biomedical Engineering, Anatomy, Physiology, Surgery, Brain Ischemia, Stroke, Hemodynamics, middle cerebral artery occlusion, cerebral hemodynamics, perfusion monitoring, Laser Doppler, intracranial collaterals, ischemic penumbra, rat, animal model

 JoVE Clinical and Translational Medicine

Utilizing a Cranial Window to Visualize the Middle Cerebral Artery During Endothelin-1 Induced Middle Cerebral Artery Occlusion


JoVE 50015

1, 2, 3, 2, 1

1Department of Physiology and Functional Genomics, University of Florida, 2Department of Neurosurgery, McKnight Brain Institute, University of Florida, 3Department of Anatomical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran

Date Published: February 22, 2013  |  doi:10.3791/50015  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Neurobiology, Neuroscience, Biomedical Engineering, Anatomy, Physiology, Genetics, Genomics, Surgery, Ischemic stroke, Cerebrovascular, Brain, Cranial window, Rat, Endothelin-1, temporal craniectomy, animal model

 JoVE Clinical and Translational Medicine

In vivo Measurement of the Mouse Pulmonary Endothelial Surface Layer


JoVE 50322

, ,

Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado School of Medicine

Date Published: February 22, 2013  |  doi:10.3791/50322  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Cellular Biology, Anatomy, Physiology, Biomedical Engineering, Biophysics, Surgery, Endothelium, Vascular, Inflammation, Pulmonary Circulation, Intravital Microscopy, endothelial surface layer, endothelial, glycocalyx, pulmonary microvasculature, catheter, tracheostomy, venous, catheterization, lung injury, mouse, animal model

 JoVE Clinical and Translational Medicine

Catheter Ablation in Combination With Left Atrial Appendage Closure for Atrial Fibrillation


JoVE 3818

, , , ,

Department of Cardiology, St. Antonius Hospital, The Netherlands

Date Published: February 26, 2013  |  doi:10.3791/3818  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Anatomy, Physiology, Biomedical Engineering, Immunology, Cardiology, Surgery, catheter ablation, WATCHMAN, LAA occlusion, atrial fibrillation, left atrial appendage, warfarin, oral anticoagulation alternatives, catheterization, ischemia, stroke, heart, vein, clinical, surgical device, surgical techniques, Vitamin K antagonist

 JoVE Clinical and Translational Medicine

Direct Pressure Monitoring Accurately Predicts Pulmonary Vein Occlusion During Cryoballoon Ablation


JoVE 50247

1, 1, 2, 1, 1, 1

1Department of Cardiac Electrophysiology, Piedmont Heart Institute, 2Cardiac Rhythm, Medtronic Inc.

Date Published: February 26, 2013  |  doi:10.3791/50247  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Anatomy, Physiology, Cardiology, Biomedical Engineering, Surgery, Cardiovascular System, Cardiovascular Diseases, Surgical Procedures, Operative, Investigative Techniques, Atrial fibrillation, Cryoballoon Ablation, Pulmonary Vein Occlusion, Pulmonary Vein Isolation, electrophysiology, catheterizatoin, heart, vein, clinical, surgical device, surgical techniques

 JoVE Clinical and Translational Medicine

A Zebrafish Model of Diabetes Mellitus and Metabolic Memory


JoVE 50232

1, 1, 2

1Dr. William M. Scholl College of Podiatric Medicine, Rosalind Franklin University of Medicine and Science, 2Chicago Medical School, Rosalind Franklin University of Medicine and Science

Date Published: February 28, 2013  |  doi:10.3791/50232  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Genetics, Genomics, Physiology, Anatomy, Biomedical Engineering, Metabolomics, Zebrafish, diabetes, metabolic memory, tissue regeneration, streptozocin, epigenetics, Danio rerio, animal model, diabetes mellitus, diabetes, drug discovery, hyperglycemia

 JoVE Bioengineering

Density Gradient Multilayered Polymerization (DGMP): A Novel Technique for Creating Multi-compartment, Customizable Scaffolds for Tissue Engineering


JoVE 50018

1, 2, 1, 3, 3, 1,2

1Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, 2Biomedical Sciences Program, University of California, San Diego, 3Department of Bioengineering, University of California, San Diego

Date Published: February 12, 2013  |  doi:10.3791/50018  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Biomedical Engineering, Tissue Engineering, Cell Culture Techniques, Tissue Culture Techniques, hydrogels, life sciences, bioengineering (general), Scaffolds, hydrogels, cell culture, polyethylene glycol, RGDS

 JoVE Bioengineering

Quantitative FRET (Förster Resonance Energy Transfer) Analysis for SENP1 Protease Kinetics Determination


JoVE 4430

,

Department of Bioengineering, Bourns College of Engineering, University of California, Riverside

Date Published: February 21, 2013  |  doi:10.3791/4430  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Biochemistry, Molecular Biology, Proteins, Quantitative FRET analysis, QFRET, enzyme kinetics analysis, SENP, SUMO, plasmid, protein expression, protein purification, protease assay, quantitative analysis

 JoVE Bioengineering

Quantifying the Mechanical Properties of the Endothelial Glycocalyx with Atomic Force Microscopy


JoVE 50163

,

Department of Biomedical Engineering, University of Rochester

Date Published: February 21, 2013  |  doi:10.3791/50163  |  Section: JoVE Bioengineering

Keywords: Biomedical Engineering, Bioengineering, Cellular Biology, Biophysics, Molecular Biology, Endothelium, Vascular, Membrane Glycoproteins, Receptors, Leukocyte-Adhesion, bioengineering (general), glycocalyx, mechanical properties, atomic force microscopy, ATM, Endothelial cells, leukocytes, cell wall, cell culture, microscopy, imaging

 JoVE Applied Physics

Bringing the Visible Universe into Focus with Robo-AO


JoVE 50021

1,2, 1, 3, 4, 2, 1, 4, 4, 4, 1, 1, 5, 1,2, 2, 6, 4

1Caltech Optical Observatories, California Institute of Technology, 2Department of Astronomy, California Institute of Technology, 3Dunlap Institute for Astronomy and Astrophysics, University of Toronto, 4Inter-University Centre for Astronomy & Astrophysics, 5Observatories of the Carnegie Institution for Science, 6Benoziyo Center for Astrophysics, Weizmann Institute of Science

Date Published: February 12, 2013  |  doi:10.3791/50021  |  Section: JoVE Applied Physics

Keywords: Physics, Astronomy, Mechanical Engineering, Astrophysics, Optics, Adaptive optics, lasers, wavefront sensing, robotics, stars, galaxies, imaging, supernova, telescopes

 JoVE Applied Physics

Characterization of Surface Modifications by White Light Interferometry: Applications in Ion Sputtering, Laser Ablation, and Tribology Experiments


JoVE 50260

1, 2, 3, 1, 1, 1

1Materials Science Division, Argonne National Laboratory, 2Energy Systems Division, Argonne National Laboratory, 3MassThink LLC

Date Published: February 27, 2013  |  doi:10.3791/50260  |  Section: JoVE Applied Physics

Keywords: Materials Science, Physics, Ion Beams (nuclear interactions), Light Reflection, Optical Properties, Semiconductor Materials, White Light Interferometry, Ion Sputtering, Laser Ablation, Femtosecond Lasers, Depth Profiling, Time-of-flight Mass Spectrometry, Tribology, Wear Analysis, Optical Profilometry, wear, friction, atomic force microscopy, AFM, scanning electron microscopy, SEM, imaging, visualization

 JoVE Applied Physics

Fabrication of Nano-engineered Transparent Conducting Oxides by Pulsed Laser Deposition


JoVE 50297

1, 1, 1,2, 1,2, 1

1Department of Energy and NEMAS - Center for NanoEngineered Materials and Surfaces, Politecnico di Milano, 2Center for Nano Science and Technology, Instituto Italiano di Tecnologia

Date Published: February 27, 2013  |  doi:10.3791/50297  |  Section: JoVE Applied Physics

Keywords: Materials Science, Physics, Nanotechnology, Nanoengineering, Oxides, thin films, thin film theory, deposition and growth, Pulsed laser Deposition (PLD), Transparent conducting oxides (TCO), Hierarchically organized Nanostructured oxides, Al doped ZnO (AZO) films, enhanced light scattering capability, gases, deposition, nanoporus, nanoparticles, Van der Pauw, scanning electron microscopy, SEM

 JoVE Chemistry

LabVIEW-operated Novel Nanoliter Osmometer for Ice Binding Protein Investigations


JoVE 4189

1,2, 1

1Institute of Biochemistry, Food Science, and Nutrition , The Robert H. Smith Faculty of Agriculture, Food, and Environment, The Hebrew University of Jerusalem, 2Department of Physics and Astronomy, Ohio University

Date Published: February 4, 2013  |  doi:10.3791/4189  |  Section: JoVE Chemistry

Keywords: Biochemistry, Chemistry, Physics, Biophysics, Bioengineering, Microbiology, Proteins, Ice binding proteins, IBP, antifreeze proteins, thermal hysteresis proteins, TH, ice structuring proteins, recrystallization inhibition proteins, nanoliter osmometer, LabVIEW, temperature control, microscopy stage, nano, Gibbs-Thomson effect, microfluidics, microscale chemistry

 JoVE Chemistry

Rapid Colorimetric Assays to Qualitatively Distinguish RNA and DNA in Biomolecular Samples


JoVE 50225

,

Department of Chemistry, University of Virginia

Date Published: February 4, 2013  |  doi:10.3791/50225  |  Section: JoVE Chemistry

Keywords: Chemistry, Biochemistry, Chemical Biology, Genetics, Molecular Biology, Cellular Biology, Nucleic Acids, DNA, RNA, Proteins, analytical chemistry, Benedict's assay, Bial's orcinol assay, Dische's diphenylamine assay, colorimetric assay, reducing sugar, purification, transcription, reaction, assay

 JoVE Chemistry

Preparation and Use of Samarium Diiodide (SmI2) in Organic Synthesis: The Mechanistic Role of HMPA and Ni(II) Salts in the Samarium Barbier Reaction


JoVE 4323

, ,

Department of Chemistry, Lehigh University

Date Published: February 4, 2013  |  doi:10.3791/4323  |  Section: JoVE Chemistry

Keywords: Chemistry, Organic Chemistry, Chemical Engineering, Biochemistry, Samarium diiodide, Sml2, Samarium-Barbier Reaction, HMPA, hexamethylphosphoramide, Ni(II), Nickel(II) acetylacetonate, nickel, samarium, iodine, additives, synthesis, catalyst, reaction, synthetic organic chemistry

 JoVE Chemistry

Origami Inspired Self-assembly of Patterned and Reconfigurable Particles


JoVE 50022

1, 1, 1,2

1Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, 2Department of Chemistry, The Johns Hopkins University

Date Published: February 4, 2013  |  doi:10.3791/50022  |  Section: JoVE Chemistry

Keywords: Chemistry, Chemical Engineering, Biomolecular Engineering, Materials Science, Physics, Nanotechnology, Molecular Self-assembly, Electrochemistry, Folding, three dimensional, lithography, colloid, patchy particles, particles, nanoparticles, robotics, drug delivery, microfabrication, nanofabrication, nano, assembly, synthesis, reaction, origami

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