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Table of Contents

Table of Contents: Issue 72, February 2013

Jump to:  JoVE Editorial (1) JoVE Biology (11) JoVE Neuroscience (6) JoVE Immunology and Infection (6) JoVE Clinical and Translational Medicine (13) JoVE Bioengineering (3) JoVE Applied Physics (3) JoVE Chemistry (4)
 JoVE Biology

Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization

1Department of Pathology, New York University School of Medicine, 2New York University Center for Health Informatics and Bioinformatics, 3NYU Cancer Institute, 4Department of Pathology and Yale Cancer Center, Yale University School of Medicine


JoVE 50087

Date Published: February 3, 2013  |  doi:10.3791/50087  |  Section: JoVE Biology

 JoVE Biology

Evaluation of Muscle Function of the Extensor Digitorum Longus Muscle Ex vivo and Tibialis Anterior Muscle In situ in Mice

1Department of Molecular Microbiology and Immunology, School of Medicine, University of Missouri


JoVE 50183

Date Published: February 9, 2013  |  doi:10.3791/50183  |  Section: JoVE Biology

 JoVE Biology

Using RNA-mediated Interference Feeding Strategy to Screen for Genes Involved in Body Size Regulation in the Nematode C. elegans

1Department of Science, Borough of Manhattan Community College, City Universtiy of New York (CUNY), 2Department of Biology, Queens College, The City University of New York (CUNY), 3Biochemistry Program, The Graduate Center, Queens College, The City University of New York (CUNY)


JoVE 4373

Date Published: February 13, 2013  |  doi:10.3791/4373  |  Section: JoVE Biology

 JoVE Biology

RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells

1Children's Mercy Hospital and Clinics, School of Medicine, University of Missouri-Kansas City


JoVE 4393

Date Published: February 13, 2013  |  doi:10.3791/4393  |  Section: JoVE Biology

 JoVE Biology

Measuring Cation Transport by Na,K- and H,K-ATPase in Xenopus Oocytes by Atomic Absorption Spectrophotometry: An Alternative to Radioisotope Assays

1Institute of Chemistry, Technical University of Berlin, 2The Vollum Institute, Oregon Health & Science University


JoVE 50201

Date Published: February 19, 2013  |  doi:10.3791/50201  |  Section: JoVE Biology

 JoVE Biology

Protease- and Acid-catalyzed Labeling Workflows Employing 18O-enriched Water

1Boston Biomedical Research Institute


JoVE 3891

Date Published: February 20, 2013  |  doi:10.3791/3891  |  Section: JoVE Biology

 JoVE Biology

Microgavage of Zebrafish Larvae

1Department of Cell and Molecular Physiology, University of North Carolina at Chapel Hill


JoVE 4434

Date Published: February 20, 2013  |  doi:10.3791/4434  |  Section: JoVE Biology

 JoVE Biology

Design and Use of Multiplexed Chemostat Arrays

1Department of Genome Sciences, University of Washington


JoVE 50262

Date Published: February 23, 2013  |  doi:10.3791/50262  |  Section: JoVE Biology

 JoVE Biology

Serial Enrichment of Spermatogonial Stem and Progenitor Cells (SSCs) in Culture for Derivation of Long-term Adult Mouse SSC Lines

1Department of Surgery, Weill Cornell Medical College


JoVE 50017

Date Published: February 25, 2013  |  doi:10.3791/50017  |  Section: JoVE Biology

 JoVE Biology

Trajectory Data Analyses for Pedestrian Space-time Activity Study

1School of Environmental and Life Sciences, Kean University, 2Department of Geography, University of Wisconsin-Madison


JoVE 50130

Date Published: February 25, 2013  |  doi:10.3791/50130  |  Section: JoVE Biology

 JoVE Biology

Rapid Genetic Analysis of Epithelial-Mesenchymal Signaling During Hair Regeneration

1Program in Epithelial Biology, Stanford University School of Medicine


JoVE 4344

Date Published: February 28, 2013  |  doi:10.3791/4344  |  Section: JoVE Biology

 JoVE Neuroscience

Intact Histological Characterization of Brain-implanted Microdevices and Surrounding Tissue

1Weldon School of Biomedical Engineering, Purdue University, 2Department of Biological Sciences, Purdue University


JoVE 50126

Date Published: February 11, 2013  |  doi:10.3791/50126  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Neonatal Subventricular Zone Electroporation

1Department of Neurosurgery and Cellular & Molecular Physiology, Yale University School of Medicine


JoVE 50197

Date Published: February 11, 2013  |  doi:10.3791/50197  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Vertical T-maze Choice Assay for Arthropod Response to Odorants

1Entomology and Nematology Department, University of Florida


JoVE 50229

Date Published: February 14, 2013  |  doi:10.3791/50229  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Appetitive Associative Olfactory Learning in Drosophila Larvae

1Department of Biology, University of Konstanz, 2Department of Biology, University of Fribourg


JoVE 4334

Date Published: February 18, 2013  |  doi:10.3791/4334  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Detection of Protein Palmitoylation in Cultured Hippocampal Neurons by Immunoprecipitation and Acyl-Biotin Exchange (ABE)

1Department of Cellular and Physiological Sciences, Brain Research Centre, University of British Columbia


JoVE 50031

Date Published: February 18, 2013  |  doi:10.3791/50031  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Identification of Olfactory Volatiles using Gas Chromatography-Multi-unit Recordings (GCMR) in the Insect Antennal Lobe

1Department of Biology, University of Washington


JoVE 4381

Date Published: February 24, 2013  |  doi:10.3791/4381  |  Section: JoVE Neuroscience

 JoVE Immunology and Infection

Clinical Application of Sleeping Beauty and Artificial Antigen Presenting Cells to Genetically Modify T Cells from Peripheral and Umbilical Cord Blood

1Division of Pediatrics, U.T. MD Anderson Cancer Center, 2Department of Stem Cell Transplantation and Cellular Therapy, U.T. MD Anderson Cancer Center


JoVE 50070

Date Published: February 1, 2013  |  doi:10.3791/50070  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells

1Department of Chemical and Biomolecular Engineering, University of Houston, 2Division of Pediatrics, Research Unit 907, University of Texas MD Anderson Cancer Center


JoVE 50058

Date Published: February 2, 2013  |  doi:10.3791/50058  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Staphylococcus aureus Growth using Human Hemoglobin as an Iron Source

1Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical School


JoVE 50072

Date Published: February 7, 2013  |  doi:10.3791/50072  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Establishing a Liquid-covered Culture of Polarized Human Airway Epithelial Calu-3 Cells to Study Host Cell Response to Respiratory Pathogens In vitro

1National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention (CDC)


JoVE 50157

Date Published: February 7, 2013  |  doi:10.3791/50157  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Analysis of the Solvent Accessibility of Cysteine Residues on Maize rayado fino virus Virus-like Particles Produced in Nicotiana benthamiana Plants and Cross-linking of Peptides to VLPs

1Plant Sciences Institute, Agricultural Research Service, United States Department of Agriculture, 2Molecular Plant Pathology Laboratory, Agricultural Research Service, United States Department of Agriculture


JoVE 50084

Date Published: February 14, 2013  |  doi:10.3791/50084  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

The Citrobacter rodentium Mouse Model: Studying Pathogen and Host Contributions to Infectious Colitis

1Division of Gastroenterology, BC Children's Hospital


JoVE 50222

Date Published: February 19, 2013  |  doi:10.3791/50222  |  Section: JoVE Immunology and Infection

 JoVE Clinical and Translational Medicine

Identification of Sleeping Beauty Transposon Insertions in Solid Tumors using Linker-mediated PCR

1Department of Obstetrics, Gynecology & Women's Health, Masonic Cancer Center, University of Minnesota, Minneapolis, 2Department of Genetics, Cell Biology & Development, Center for Genome Engineering, University of Minnesota, Minneapolis


JoVE 50156

Date Published: February 1, 2013  |  doi:10.3791/50156  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Time-lapse Imaging of Primary Preneoplastic Mammary Epithelial Cells Derived from Genetically Engineered Mouse Models of Breast Cancer

1Department of Oncology, Georgetown University, 2Lombardi Comprehensive Cancer Center, Georgetown University, 3Stem Cell Dynamics, Helmholtz Zentrum München - German Research Center for Environmental Health, 4Department of Medicine, Georgetown University, 5Department of Nanobiomedical Science and WCU Research Center of Nanobiomedical Science, Dankook University


JoVE 50198

Date Published: February 8, 2013  |  doi:10.3791/50198  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Angiogenesis in the Ischemic Rat Lung

1Johns Hopkins Asthma and Allergy Center, Division of Pulmonary and Critical Care Medicine, Johns Hopkins University


JoVE 50217

Date Published: February 8, 2013  |  doi:10.3791/50217  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Cardiac Stress Test Induced by Dobutamine and Monitored by Cardiac Catheterization in Mice

1Instituto de Ciencias, Facultad de Medicina, Clínica Alemana Universidad del Desarrollo


JoVE 50050

Date Published: February 10, 2013  |  doi:10.3791/50050  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Murine Fetal Echocardiography

1Department of Medicine, Section of Cardiology, Institute for Cardiovascular Research, University of Chicago


JoVE 4416

Date Published: February 15, 2013  |  doi:10.3791/4416  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Experimental Human Pneumococcal Carriage

1Respiratory Infection Group, Liverpool School of Tropical Medicine, 2Royal Liverpool and Broadgreen, University Hospital Trust, 3Comprehensive Local Research Network, 4NIHR Biomedical Research Centre in Microbial Diseases, Royal Liverpool and Broadgreen University Hospitals NHS Trust, 5Institute of Lung Health, Respiratory Biomedical Unit, University Hospitals of Leicester NHS Trust & University of Leicester, 6Department of Clinical Infection Microbiology & Immunology, Institute of Infection & Global Health, University of Liverpool


JoVE 50115

Date Published: February 15, 2013  |  doi:10.3791/50115  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Endothelin-1 Induced Middle Cerebral Artery Occlusion Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Rat

1Department of Neurosurgery, University of Florida, 2Department of Anatomical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran, 3Department of Physiology and Functional Genomics, University of Florida, 4Department of Neurology, University of Florida


JoVE 50014

Date Published: February 16, 2013  |  doi:10.3791/50014  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Optimized System for Cerebral Perfusion Monitoring in the Rat Stroke Model of Intraluminal Middle Cerebral Artery Occlusion

1Department of Neuroscience and Biomedical Technologies, University of Milano Bicocca


JoVE 50214

Date Published: February 17, 2013  |  doi:10.3791/50214  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Utilizing a Cranial Window to Visualize the Middle Cerebral Artery During Endothelin-1 Induced Middle Cerebral Artery Occlusion

1Department of Physiology and Functional Genomics, University of Florida, 2Department of Neurosurgery, McKnight Brain Institute, University of Florida, 3Department of Anatomical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran


JoVE 50015

Date Published: February 22, 2013  |  doi:10.3791/50015  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

In vivo Measurement of the Mouse Pulmonary Endothelial Surface Layer

1Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado School of Medicine


JoVE 50322

Date Published: February 22, 2013  |  doi:10.3791/50322  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Catheter Ablation in Combination With Left Atrial Appendage Closure for Atrial Fibrillation

1Department of Cardiology, St. Antonius Hospital, The Netherlands


JoVE 3818

Date Published: February 26, 2013  |  doi:10.3791/3818  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Direct Pressure Monitoring Accurately Predicts Pulmonary Vein Occlusion During Cryoballoon Ablation

1Department of Cardiac Electrophysiology, Piedmont Heart Institute, 2Cardiac Rhythm, Medtronic Inc.


JoVE 50247

Date Published: February 26, 2013  |  doi:10.3791/50247  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

A Zebrafish Model of Diabetes Mellitus and Metabolic Memory

1Dr. William M. Scholl College of Podiatric Medicine, Rosalind Franklin University of Medicine and Science, 2Chicago Medical School, Rosalind Franklin University of Medicine and Science


JoVE 50232

Date Published: February 28, 2013  |  doi:10.3791/50232  |  Section: JoVE Clinical and Translational Medicine

 JoVE Bioengineering

Density Gradient Multilayered Polymerization (DGMP): A Novel Technique for Creating Multi-compartment, Customizable Scaffolds for Tissue Engineering

1Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, 2Biomedical Sciences Program, University of California, San Diego, 3Department of Bioengineering, University of California, San Diego


JoVE 50018

Date Published: February 12, 2013  |  doi:10.3791/50018  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Quantitative FRET (Förster Resonance Energy Transfer) Analysis for SENP1 Protease Kinetics Determination

1Department of Bioengineering, Bourns College of Engineering, University of California, Riverside


JoVE 4430

Date Published: February 21, 2013  |  doi:10.3791/4430  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Quantifying the Mechanical Properties of the Endothelial Glycocalyx with Atomic Force Microscopy

1Department of Biomedical Engineering, University of Rochester


JoVE 50163

Date Published: February 21, 2013  |  doi:10.3791/50163  |  Section: JoVE Bioengineering

 JoVE Applied Physics

Bringing the Visible Universe into Focus with Robo-AO

1Caltech Optical Observatories, California Institute of Technology, 2Department of Astronomy, California Institute of Technology, 3Dunlap Institute for Astronomy and Astrophysics, University of Toronto, 4Inter-University Centre for Astronomy & Astrophysics, 5Observatories of the Carnegie Institution for Science, 6Benoziyo Center for Astrophysics, Weizmann Institute of Science


JoVE 50021

Date Published: February 12, 2013  |  doi:10.3791/50021  |  Section: JoVE Applied Physics

 JoVE Applied Physics

Characterization of Surface Modifications by White Light Interferometry: Applications in Ion Sputtering, Laser Ablation, and Tribology Experiments

1Materials Science Division, Argonne National Laboratory, 2Energy Systems Division, Argonne National Laboratory, 3MassThink LLC


JoVE 50260

Date Published: February 27, 2013  |  doi:10.3791/50260  |  Section: JoVE Applied Physics

 JoVE Applied Physics

Fabrication of Nano-engineered Transparent Conducting Oxides by Pulsed Laser Deposition

1Department of Energy and NEMAS - Center for NanoEngineered Materials and Surfaces, Politecnico di Milano, 2Center for Nano Science and Technology, Instituto Italiano di Tecnologia


JoVE 50297

Date Published: February 27, 2013  |  doi:10.3791/50297  |  Section: JoVE Applied Physics

 JoVE Chemistry

LabVIEW-operated Novel Nanoliter Osmometer for Ice Binding Protein Investigations

1Institute of Biochemistry, Food Science, and Nutrition , The Robert H. Smith Faculty of Agriculture, Food, and Environment, The Hebrew University of Jerusalem, 2Department of Physics and Astronomy, Ohio University


JoVE 4189

Date Published: February 4, 2013  |  doi:10.3791/4189  |  Section: JoVE Chemistry

 JoVE Chemistry

Rapid Colorimetric Assays to Qualitatively Distinguish RNA and DNA in Biomolecular Samples

1Department of Chemistry, University of Virginia


JoVE 50225

Date Published: February 4, 2013  |  doi:10.3791/50225  |  Section: JoVE Chemistry

 JoVE Chemistry

Preparation and Use of Samarium Diiodide (SmI2) in Organic Synthesis: The Mechanistic Role of HMPA and Ni(II) Salts in the Samarium Barbier Reaction

1Department of Chemistry, Lehigh University


JoVE 4323

Date Published: February 4, 2013  |  doi:10.3791/4323  |  Section: JoVE Chemistry

 JoVE Chemistry

Origami Inspired Self-assembly of Patterned and Reconfigurable Particles

1Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, 2Department of Chemistry, The Johns Hopkins University


JoVE 50022

Date Published: February 4, 2013  |  doi:10.3791/50022  |  Section: JoVE Chemistry

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