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The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.

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Table of Contents

Table of Contents: Issue 41, July 2010

Jump to:  JoVE General (17) JoVE Neuroscience (8) JoVE Immunology and Infection (4) JoVE Clinical and Translational Medicine (2)
 JoVE General

PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins


JoVE 2034

1, 2, 3, 3, 4, 4, 1, 1, 2, 2, 1, 1, 5, 3, 1

1Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, Rockefeller University, 2Berlin Institute for Medical Systems Biology, Max-Delbrück-Center for Molecular Medicine, 3Biozentrum der Universität Basel and Swiss Institute of Bioinformatics (SIB), 4Biozentrum der Universität Basel and Swiss Institute of Bioinformatics (SIB), 5Genomics Resource Center, Rockefeller University

Date Published: July 2, 2010  |  doi:10.3791/2034  |  Section: JoVE General

Keywords: Cellular Biology, UV crosslinking, RNA binding proteins, RNA binding motif, 4-thiouridine, 6-thioguanosine

 JoVE General

Isolating Stem Cells from Soft Musculoskeletal Tissues


JoVE 2011

1,2,3,4, 1, 1,2,3,4,5

1Stem Cell Research Center, Childrens Hospital of Pittsburgh of UPMC, 2Department of Bioengineering, University of Pittsburgh, 3Department of Orthopedic Surgery, University of Pittsburgh, 4Department of Pathology, University of Pittsburgh, 5Department of Molecular Genetics & Biochemistry, University of Pittsburgh

Date Published: July 5, 2010  |  doi:10.3791/2011  |  Section: JoVE General

Keywords: Cellular Biology, Adult stem cells, isolation, softy tissue, adhesion

 JoVE General

Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets


JoVE 2101

1, 2, 1, 1, 3, 2, 1

1Department of Biochemistry and Molecular Genetics, University of Illinois Chicago - UIC, 2Research Unit on Biomedical Informatics, Universitat Pompeu Fabra, 3Genome Technology Core, Whitehead Institute for Biomedical Research

Date Published: July 7, 2010  |  doi:10.3791/2101  |  Section: JoVE General

Keywords: Biochemistry, chromatin immunoprecipitation, ChIP-Seq, RBP2, JARID1A, KDM5A, isoform-specific recruitment

 JoVE General

Xenotransplantation of Human Stem Cells into the Chicken Embryo


JoVE 2071

1, 1, 1, 1,2

1Department of Physiology, University of Oslo, 2Norwegian Center for Stem Cell Research, University of Oslo

Date Published: July 11, 2010  |  doi:10.3791/2071  |  Section: JoVE General

Keywords: Cellular Biology, chicken embryo, xenotransplantation, transplantation, human stem cells, differentiation, regeneration, spinal cord, cell therapy

 JoVE General

Transplantation of GFP-expressing Blastomeres for Live Imaging of Retinal and Brain Development in Chimeric Zebrafish Embryos


JoVE 1924

1, 1,2

1Department of Ophthalmology, University of Pittsburgh, 2Department of Microbiology and Molecular Genetics, University of Pittsburgh

Date Published: July 19, 2010  |  doi:10.3791/1924  |  Section: JoVE General

Keywords: Developmental Biology, transformation, fluorescence donor fish, live imaging, zebrafish, blastomeres, embryo, GFP

 JoVE General

In situ Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells


JoVE 1958

1, 1, 1, 2, 1

1Department of Biochemistry, School of Medical Sciences, University of Bristol, 2Division of Immunity and Infection, School of Medicine, University of Birmingham

Date Published: July 23, 2010  |  doi:10.3791/1958  |  Section: JoVE General

Keywords: cellular biology, protein localisation, subcellular fractionation, in situ, chromatin, nuclear matrix

 JoVE General

Primer-Free Aptamer Selection Using A Random DNA Library


JoVE 2039

1, 1, 1, 2, 2, 3,4

1Department of Pathology, Hershey Medical Center, Pennsylvania State University, 2Department of Chemistry, Pennsylvania State University, 3Departments of Pathology, and Biochemistry and Molecular Biology, Hershey Medical Center, Pennsylvania State University, 4Materials Research Institute, Pennsylvania State University

Date Published: July 26, 2010  |  doi:10.3791/2039  |  Section: JoVE General

Keywords: Cellular Biology, aptamer, selection, S100B, sandwich

 JoVE General

Deficient Pms2, ERCC1, Ku86, CcOI in Field Defects During Progression to Colon Cancer


JoVE 1931

1, 1, 1, 1, 1, 1, 2,3, 1,4, 3, 5, 5, 5, 1

1Department of Cell Biology and Anatomy, College of Medicine, University of Arizona, Tucson, 2Southern Arizona Veterans Affairs Health Care System, Tucson, AZ, 3Department of Surgery, College of Medicine, University of Arizona, Tucson, 4Biomedical Diagnostics and Research, Tucson, AZ, 5Department of Medicine, College of Medicine, University of Arizona, Tucson

Date Published: July 28, 2010  |  doi:10.3791/1931  |  Section: JoVE General

Keywords: Cellular Biology, DNA Repair, Apoptosis, Field Defect, Colon Cancer, Pms2, ERCC1, Cytochrome c Oxidase Subunit I, Ku86, Immunohistochemistry, Cancer Resection

 JoVE General

Preparation of Rat Tail Tendons for Biomechanical and Mechanobiological Studies


JoVE 2176

, , , ,

Groupe PERSEUS, Faculté de Génie Département de génie mécanique, Université de Sherbrooke

Date Published: July 30, 2010  |  doi:10.3791/2176  |  Section: JoVE General

Keywords: bioengineering, Rat tail tendon, extraction, cross-section, optic micrometer, anchors, bioreactor, biomechanics, mechanobiology

 JoVE Neuroscience

DiOLISTIC Labeling of Neurons from Rodent and Non-human Primate Brain Slices


JoVE 2081

1, 2, 3, 3, 1

1Section on Neuronal Structure, Laboratory for Integrative Neuroscience, NIAAA, NIH, 2Department Physiology and Pharmacology, Wake Forest University Health Sciences, 3Oregon National Primate Research Center, Division of Neuroscience, Oregon Health and Science University

Date Published: July 6, 2010  |  doi:10.3791/2081  |  Section: JoVE Neuroscience

Keywords: JoVE Neuroscience, gene gun, dendritic spine, dendrite branching, neuronal morphology, diI, mouse brain, cynomogus monkey brain, fluorescent labeling

 JoVE Neuroscience

The Vermicelli and Capellini Handling Tests: Simple quantitative measures of dexterous forepaw function in rats and mice


JoVE 2076

1, 2, 3, 4, 5, 1,2

1Institute for Neuroscience, University of Texas at Austin, 2Department of Psychology, University of Texas at Austin, 3Department of Neurology, University of Florida, 4Department of Psychiatry, University of Texas Southwestern Medical Center, 5Department of Neuroscience, McKnight Brain Institute, University of Florida

Date Published: July 21, 2010  |  doi:10.3791/2076  |  Section: JoVE Neuroscience

Keywords: JoVE Neuroscience, manual dexterity, food handling, pasta, rodent models, upper extremity impairment

 JoVE Neuroscience

Assessment of Ultrasonic Vocalizations During Drug Self-administration in Rats


JoVE 2041

1,2, 3, 2,4, 2,4,5, 1,2,4

1College of Pharmacy, Division of Pharmacology and Toxicology, University of Texas at Austin, 2The Waggoner Center of Addiction and Alcohol Research, University of Texas at Austin, 3Department of Psychology, University of Michigan, 4Institute for Neuroscience, University of Texas at Austin, 5Department of Psychology, University of Texas at Austin

Date Published: July 22, 2010  |  doi:10.3791/2041  |  Section: JoVE Neuroscience

Keywords: JoVE Neuroscience, ultrasound, behavior, self-administration, emotionality, anticipation, reward

 JoVE Neuroscience

Labeling and Imaging Cells in the Zebrafish Hindbrain


JoVE 1976

1, 2, 1

1Department of Biological Sciences, University of Maryland, Baltimore County, 2Center for Neuroscience, Children's National Medical Center

Date Published: July 25, 2010  |  doi:10.3791/1976  |  Section: JoVE Neuroscience

Keywords: JoVE Neuroscience, development, zebrafish, embryo, brain, neural tube, microinjection, sectioning, time-lapse microscopy, confocal microscopy

 JoVE Immunology and Infection

Orthotopic Hind-Limb Transplantation in Rats


JoVE 2022

*1, *1, 1, 1, 2, 2, 1, 1, 1, 1

1Department of Visceral, Transplant, and Thoracic Surgery, Daniel Swarovski Research Laboratory, Innsbruck Medical University, 2Department of Surgery, Division of Plastic and Reconstructive Surgery, University of Pittsburgh Medical Center

Date Published: July 12, 2010  |  doi:10.3791/2022  |  Section: JoVE Immunology and Infection

Keywords: JoVE Immunology, rat, hind limb, composite tissue, reconstructive transplantation

 JoVE Immunology and Infection

In vivo Imaging of Transgenic Leishmania Parasites in a Live Host


JoVE 1980

1, 2, 3, 4, 5, 4, 6,7

1Interdisciplinary Immunology Program, University of Iowa, and the VA Medical Center, 2Department of Biochemistry, University of Iowa, and the VA Medical Center, 3Department of Internal Medicine, University of Iowa, 4Department of Molecular Microbiology, Washington University School of Medicine, 5Division of Dermatology, Harbor-UCLA Medical Center, Hanley-Hardison Research Center, 6Interdisciplinary Immunology Program, Iowa City VA Medical Center, 7Departments of Internal Medicine, Microbiology and Epidemiology, University of Iowa

Date Published: July 27, 2010  |  doi:10.3791/1980  |  Section: JoVE Immunology and Infection

Keywords: Microbiology, IVIS, Leishmania, in vivo imaging, parasite, transgenic, bioluminescence, luciferase, cutaneous leishmaniasis, visceral leishmaniasis

 JoVE Clinical and Translational Medicine

High-frequency High-resolution Echocardiography: First Evidence on Non-invasive Repeated Measure of Myocardial Strain, Contractility, and Mitral Regurgitation in the Ischemia-reperfused Murine Heart


JoVE 1781

1,2, 1,2, 1,2, 1,2, 2,3, 2

1Department of Surgery, The Ohio State University, 2Heart and Lung Research Institute, The Ohio State University, 3Department of Cardiovascular Medicine, The Ohio State University

Date Published: July 9, 2010  |  doi:10.3791/1781  |  Section: JoVE Clinical and Translational Medicine

Keywords: JoVE Medicine, ischemia-reperfused murine heart, high frequency ultrasound, heart contractility (dP/dt), mitral regurgitation

 JoVE Clinical and Translational Medicine

Murine Bioluminescent Hepatic Tumour Model


JoVE 1977

1, 1, 2, 2, 3, 1, 1

1Cork Cancer Research Centre, Mercy University Hospital and Leslie C. Quick Jnr. Laboratory, University College Cork, 2Department of Computer Science, University College Cork, 3South Infirmary Victoria University Hospital

Date Published: July 17, 2010  |  doi:10.3791/1977  |  Section: JoVE Clinical and Translational Medicine

Keywords: JoVE Medicine, Cancer, Therapy, Liver, Orthotopic, Metastasis

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