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The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.

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Table of Contents

Table of Contents: Issue 52, June 2011

Jump to:  JoVE Editorial (1) JoVE General (15) JoVE Neuroscience (11) JoVE Immunology and Infection (10) JoVE Clinical and Translational Medicine (8) JoVE Bioengineering (9) JoVE Errata (1)
 JoVE General

Imaging Glycans in Zebrafish Embryos by Metabolic Labeling and Bioorthogonal Click Chemistry


JoVE 2686

1, 1, 1, 2, 3, 1

1Department of Biochemistry, Albert Einstein College of Medicine, Yeshiva University, 2Macromolecular Therapeutics Development Facility, Albert Einstein College of Medicine, Yeshiva University, 3Developmental and Molecular Biology, Albert Einstein College of Medicine, Yeshiva University

Date Published: June 6, 2011  |  doi:10.3791/2686  |  Section: JoVE General

Keywords: Developmental Biology, click chemistry, chemical glycobiology, fucosylated glycans, embryogenesis, microinjection

 JoVE General

Modified ES / OP9 Co-Culture Protocol Provides Enhanced Characterization of Hematopoietic Progeny


JoVE 2559

1, 2, 1

1Department of Medicine, Hematology-Oncology, University of California, Los Angeles, 2Department of Biological Chemistry, University of California, Los Angeles

Date Published: June 7, 2011  |  doi:10.3791/2559  |  Section: JoVE General

Keywords: Developmental Biology, Embryonic stem cell, hematopoiesis, OP9, co-culture, differentiation

 JoVE General

Microwave-assisted One-pot Synthesis of N-succinimidyl-4-[18F]fluorobenzoate ([18F]SFB)


JoVE 2755

*1,2,3, *1,2,3, 1,2,3, 4, 5, 1,2,3

1Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California at Los Angeles, 2Crump Institute for Molecular Imaging, David Geffen School of Medicine, University of California at Los Angeles, 3California NanoSystems Institute, University of California at Los Angeles, 4Nuclear Medicine, PET Center, Shanghai Medical Collegea, Fudan University, 5Electronics and Information Engineering, College of Electronics and Information Engineering, Wuhan Textile University

Date Published: June 28, 2011  |  doi:10.3791/2755  |  Section: JoVE General

Keywords: Molecular Biology, Radiolabeling, microwave, radiochemistry, fluorine-18, one-pot synthesis, [18F]SFB

 JoVE General

Activation of Apoptosis by Cytoplasmic Microinjection of Cytochrome c


JoVE 2773

1, 2, 1

1Department of Cell and Developmental Biology, Neuroscience Center, University of North Carolina, 2Curriculum in Neurobiology, Neuroscience Center, University of North Carolina

Date Published: June 29, 2011  |  doi:10.3791/2773  |  Section: JoVE General

Keywords: Cellular Biology, Microinjection, apoptosis, cytochrome c, fibroblasts, neurons

 JoVE General

Insulin Injection and Hemolymph Extraction to Measure Insulin Sensitivity in Adult Drosophila melanogaster


JoVE 2722

1, 2

1Department of Biology, State University of New York, 2Allied Health Sciences, University of Connecticut

Date Published: June 30, 2011  |  doi:10.3791/2722  |  Section: JoVE General

Keywords: Physiology, insulin injection, hemolymph, insulin tolerance test, Drosophila insulin-like peptide (DILP), insulin-like producing cells (IPCs)

 JoVE Neuroscience

Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS


JoVE 2649

1, 1, 1, 1, 1,2, 1, 1, 1, 1

1Department of Zoology, University of Oklahoma - Norman, 2Department of Biology, Brandeis University

Date Published: June 3, 2011  |  doi:10.3791/2649  |  Section: JoVE Neuroscience

Keywords: Neuroscience, UAS, Gal4, Gal80, Flippase, FRT, Clonal analysis, Behavior, Drosophila

 JoVE Neuroscience

Modeling Neural Immune Signaling of Episodic and Chronic Migraine Using Spreading Depression In Vitro


JoVE 2910

*1, *1, 2, 1

1Department of Neurology and Committee on Neurobiology, The University of Chicago Medical Center, 2Department of Neurology, The University of Chicago Medical Center

Date Published: June 13, 2011  |  doi:10.3791/2910  |  Section: JoVE Neuroscience

Keywords: Neuroscience, innate immunity, hormesis, microglia, T-cells, hippocampus, slice culture, gene expression, laser dissection microscopy, real-time qPCR, interferon-gamma

 JoVE Neuroscience

Neuromodulation and Mitochondrial Transport: Live Imaging in Hippocampal Neurons over Long Durations


JoVE 2599

*, *, *

Department of Experimental Neurobiology, The Neurosciences Institute

Date Published: June 17, 2011  |  doi:10.3791/2599  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Mitochondria, Transport, Neuromodulation, Hippocampal neuron, Serotonin, Dopamine, Fluorescence, Time-lapse, Live imaging, Stage-top incubator

 JoVE Neuroscience

Efficient Gene Delivery into Multiple CNS Territories Using In Utero Electroporation


JoVE 2957

1, 2, 1, 1,2, 1, 2, 1

1Department of Biochemistry and Molecular Biology, Hotchkiss Brain Institute, Alberta Children’s Hospital Research Institute, University of Calgary, 2Department of Medical Genetics, Alberta Children’s Hospital Research Institute, Hotchkiss Brain Institute, University of Calgary

Date Published: June 23, 2011  |  doi:10.3791/2957  |  Section: JoVE Neuroscience

Keywords: Neuroscience, In utero electroporation, embryonic central nervous system, telencephalon, diencephalon, retina, gene delivery, mouse, gain-of-function, loss-of-function

 JoVE Neuroscience

In vivo Electroporation of Developing Mouse Retina


JoVE 2847

1, 1,2,3,4,5

1Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine, 2Department of Neurology, Johns Hopkins School of Medicine, 3Department of Ophthalmology, Johns Hopkins School of Medicine, 4Center for High-Throughput Biology, Johns Hopkins School of Medicine, 5Institute for Cell Engineering, Johns Hopkins School of Medicine

Date Published: June 24, 2011  |  doi:10.3791/2847  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Electroporation, retina, in vivo, gene expression, gain of function, loss of function

 JoVE Immunology and Infection

Direct Observation of Phagocytosis and NET-formation by Neutrophils in Infected Lungs using 2-photon Microscopy


JoVE 2659

1, 1, 1, 2, 1

1Institute for Molecular and Clinical Immunology, Otto-von-Guericke University Magdeburg, 2Department of Immunoregulation, Helmholtz Center for Infection Research

Date Published: June 2, 2011  |  doi:10.3791/2659  |  Section: JoVE Immunology and Infection

Keywords: Immunology, 2-photon microscopy, lung, neutrophil extracellular trap (NET), Aspergillus fumigatus

 JoVE Immunology and Infection

Generation and Labeling of Murine Bone Marrow-derived Dendritic Cells with Qdot Nanocrystals for Tracking Studies


JoVE 2785

*1, *2, 2, 1,2,3

1Molecular and Cell Biology Program, Ohio University, 2Department of Biomedical Sciences, College of Osteopathic Medicine, Ohio University, 3Department of Biomedical Engineering, Russ College of Engineering and Technology, Ohio University

Date Published: June 2, 2011  |  doi:10.3791/2785  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Dendritic cells, Qdot nanocrystals, labeling, cell tracking, mouse

 JoVE Immunology and Infection

Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay


JoVE 2889

, , , ,

Center for Vector Biology and Zoonotic Diseases, Department of Environmental Sciences, The Connecticut Agricultural Experiment Station

Date Published: June 9, 2011  |  doi:10.3791/2889  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Mosquito-borne viruses, mosquitoes, cell culture, surveillance

 JoVE Immunology and Infection

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency


JoVE 2953

, , , , , ,

Viral Populations and Pathogenesis lab and CNRS 3015, Institut Pasteur

Date Published: June 16, 2011  |  doi:10.3791/2953  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Polymerase fidelity, RNA virus, mutation frequency, mutagen, RNA polymerase, viral evolution

 JoVE Immunology and Infection

Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery


JoVE 2954

1, 1, 2, 3, 1

1Department of Molecular and Cellular Biology, Beckman Research Institute of City of Hope, 2Graduate School of Biological Sciences, Beckman Research Institute of City of Hope, 3Shared Resource-DNA/RNA Peptide, Beckman Research Institute of City of Hope

Date Published: June 23, 2011  |  doi:10.3791/2954  |  Section: JoVE Immunology and Infection

Keywords: Immunology, SELEX (Systematic Evolution of Ligands by EXponential enrichment), RNA aptamer, HIV-1 gp120, RNAi (RNA interference), siRNA (small interfering RNA), cell-type specific delivery

 JoVE Immunology and Infection

Antimicrobial Susceptibility Testing of Mycobacterium Tuberculosis Complex for First and Second Line Drugs by Broth Dilution in a Microtiter Plate Format


JoVE 3094

, , ,

Department of Laboratory Medicine and Pathology, Mayo Clinic

Date Published: June 24, 2011  |  doi:10.3791/3094  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Mycobacterium tuberculosis, MIC, antimicrobial susceptibility testing, first and second line drugs, microtiter plate, broth dilution

 JoVE Immunology and Infection

A Cell Free Assay System Estimating the Neutralizing Capacity of GM-CSF Antibody using Recombinant Soluble GM-CSF Receptor


JoVE 2742

1, 1, 1, 1, 2, 3, 3, 1

1Bioscience Medical Research Center, Niigata University Medical and Dental Hospital, 2First department of Internal Medicine, School of Medicine, Kyorin University, 3Neosilk Laboratory, Immuno Biological Laboratories Co., Ltd.

Date Published: June 27, 2011  |  doi:10.3791/2742  |  Section: JoVE Immunology and Infection

Keywords: Molecular Biology, GM-CSF, GM-CSF autoantibody, GM-CSF receptor α, receptor binding assay, cell free system

 JoVE Clinical and Translational Medicine

Sequencing of Bacterial Microflora in Peripheral Blood: our Experience with HIV-infected Patients


JoVE 2830

, , , ,

Department of Medicine, Surgery and Dentistry, Clinic of Infectious Diseases, San Paolo Hospital University of Milan, Italy

Date Published: June 11, 2011  |  doi:10.3791/2830  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Plasma DNA extraction, 16S rRNA gene PCR, sequencing analysis, HIV

 JoVE Clinical and Translational Medicine

A Strategy to Identify de Novo Mutations in Common Disorders such as Autism and Schizophrenia


JoVE 2534

1, 2, 3

1Centre of Excellence in Neuromics, CHUM Research Center and the Department of Medicine, Universite de Montreal, 2Center of Excellence in Neuromics, CHU Sainte Justine and CHUM Notre-Dame Research Centers, Universite de Montreal, 3Department of Medicine, Universite de Montreal

Date Published: June 15, 2011  |  doi:10.3791/2534  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, de novo mutation, complex diseases, schizophrenia, autism, rare variations, DNA sequencing

 JoVE Clinical and Translational Medicine

Mouse Complete Stasis Model of Inferior Vena Cava Thrombosis


JoVE 2738

, , , ,

Conrad Jobst Vascular Research Laboratories, Section of Vascular Surgery, University of Michigan

Date Published: June 15, 2011  |  doi:10.3791/2738  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Animal model, mouse, venous thrombosis, stasis induced thrombosis, inflammation, venous disease

 JoVE Clinical and Translational Medicine

Evaluation of Nanoparticle Uptake in Tumors in Real Time Using Intravital Imaging


JoVE 2808

1, 2, 2, 3, 2,4

1Department of Medical Biophysics, University of Western Ontario, 2London Regional Cancer Program, London Health Science Centre, 3Department of Pathology, Vanderbilt University, 4Translational Prostate Cancer Research Group, London Health Science Centre

Date Published: June 21, 2011  |  doi:10.3791/2808  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Nanoparticles, tumors, intravital imaging, avian embryo, confocal microscopy

 JoVE Clinical and Translational Medicine

Basic Surgical Techniques in the Göttingen Minipig: Intubation, Bladder Catheterization, Femoral Vessel Catheterization, and Transcardial Perfusion


JoVE 2652

1,2, 2, 2, 1, 1, 1, 1,2, 3

1Department of Neurosurgery, Aarhus University Hospital, 2Department of Neurobiology, Institute of Anatomy, Faculty of Health Sciences, Aarhus University, 3Positron Emission Tomography (PET) Centre, Aarhus University Hospital

Date Published: June 26, 2011  |  doi:10.3791/2652  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Animal model, Animal preparation, Animal handling, Sus scrofa, Swine, Tissue fixation

 JoVE Bioengineering

Bioluminescence Imaging for Assessment of Immune Responses Following Implantation of Engineered Heart Tissue (EHT)


JoVE 2605

1,2, 1, 1, 1,3, 2, 2, 1, 3, 2, 1,3

1Transplant and Stem Cell Immunobiology Lab (TSI) and CVRC, University Hospital Hamburg, University Heart Center Hamburg, 2Department of Experimental and Clinical Pharmacology and Toxicology, University Heart Center Hamburg, 3CT Surgery, Stanford University School of Medicine

Date Published: June 1, 2011  |  doi:10.3791/2605  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Engineered heart tissue, bioluminescence imaging, rejection, rats, immune response

 JoVE Bioengineering

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules


JoVE 2922

1, 1, 1, 2

1Department of Chemistry and Biochemistry, University Of California Santa Barbara, 2Department of Chemistry and Biochemistry, Program in BioMolecular Science and Engineering, University Of California Santa Barbara

Date Published: June 1, 2011  |  doi:10.3791/2922  |  Section: JoVE Bioengineering

Keywords: Bioengineering, biosensor, chemistry, detection, electrochemistry, point of care, theranostics, diagnostics, antibody, instrument, electronic

 JoVE Bioengineering

Plasma Lithography Surface Patterning for Creation of Cell Networks


JoVE 3115

1, 1, 1, 1, 1, 1,2

1Aerospace and Mechanical Engineering, University of Arizona, 2Biomedical Engineering IDP and BIO5 Institute, University of Arizona

Date Published: June 14, 2011  |  doi:10.3791/3115  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Cell Network, Surface Patterning, Self-Organization, Developmental Biology, Tissue Engineering, Nanopattern, Micropattern, Self-Assembly, Cell Guidance, Neuron

 JoVE Bioengineering

High Resolution 3D Imaging of Ex-Vivo Biological Samples by Micro CT


JoVE 2688

1, 2, 3

1Department of Molecular Genetics, Weizmann Institute of Science, 2Department of Biological Regulation, Weizmann Institute of Science, 3Department of Chemical Infrastructure, Weizmann Institute of Science

Date Published: June 21, 2011  |  doi:10.3791/2688  |  Section: JoVE Bioengineering

Keywords: Bioengineering, 3D imaging, tomography, x-ray, non invasive, ex-vivo

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