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The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.

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1: October/November 2006

Table of Contents

Table of Contents: Issue 17, July 2008

Jump to: (14)

Freezing, Thawing, and Packaging Cells for Transport

JoVE 757

Richard Ricardo, Katy Phelan

Molecular Pathology Laboratory Network, Inc

Date Published: July 2, 2008 | doi:10.3791/757 | Section: JoVE General

Keywords: Basic Protocols, Current Protocols Wiley, Freezing Cells, Cell Culture, Thawing Cells, Storage of Cells, Suspension Cells, Adherent Cells

Whole-cell Recordings of Light Evoked Excitatory Synaptic Currents in the Retinal Slice

JoVE 771

Birgit Werner¹, Paul B. Cook^1,2, Christopher L. Passaglia^1,3

¹Program in Neuroscience, Boston University, ²Department of Biology, Boston University, ³Department of Biomedical Engineering, Boston University

Date Published: July 2, 2008 | doi:10.3791/771 | Section: JoVE General

Keywords: Neuroscience, Retina, Whole-cell recording, Tiger salamander, Light-evoked currents

Staining Proteins in Gels

JoVE 760

Sean Gallagher¹, Deb Chakavarti²

¹Keck Graduate Institute of Applied Life Sciences, UVP, LLC, ²Proteomic Center, Keck Graduate Institute of Applied Life Sciences

Date Published: July 8, 2008 | doi:10.3791/760 | Section: JoVE General

Keywords: Basic Protocols, Current Protocols Wiley, Coomassie Blue Staining, Silver Staining, SYPROruby, SYPROorange, Protein Detection

Generation of Bone Marrow Derived Murine Dendritic Cells for Use in 2-photon Imaging

JoVE 773

Melanie P. Matheu¹, Debasish Sen¹, Michael D Cahalan¹, Ian Parker²

¹Department of Physiology and Biophysics, University of California, Irvine (UCI), ²Department of Neurobiology and Behaviour, University of California, Irvine (UCI)

Date Published: July 9, 2008 | doi:10.3791/773 | Section: JoVE General

Keywords: Immunology, dendritic cells, mouse, bone marrow, 2-photon imaging, cell culture

Single Cell Electroporation in vivo within the Intact Developing Brain

JoVE 705

D. Sesath Hewapathirane^1,2, Kurt Haas^1,2

¹Brain Research Centre, University of British Columbia - UBC, ²Department of Cellular and Physiological Sciences, University of British Columbia - UBC

Date Published: July 11, 2008 | doi:10.3791/705 | Section: JoVE General

Keywords: Neuroscience, electroporation, gene delivery, transfection, fluorescence labeling, neuronal imaging, micropipette

Interview: Protein Folding and Studies of Neurodegenerative Diseases

JoVE 786

Susan Lindquist

Whitehead Institute for Biomedical Research, MIT - Massachusetts Institute of Technology

Date Published: July 16, 2008 | doi:10.3791/786 | Section: JoVE General

Keywords: Neuroscience, issue 17, protein folding, brain, neuron, prion, neurodegenerative disease, yeast, screen, Translational Research

Screening for Amyloid Aggregation by Semi-Denaturing Detergent-Agarose Gel Electrophoresis

JoVE 838

Randal Halfmann^1,2,3, Susan Lindquist^1,2,3

¹Whitehead Institute for Biomedical Research, ²Department of Biology, MIT - Massachusetts Institute of Technology, ³Howard Hughes Medical Institute

Date Published: July 16, 2008 | doi:10.3791/838 | Section: JoVE General

Keywords: Basic Protocols, biochemistry, SDD-AGE, amyloid, prion, aggregate

Application of a C. elegans Dopamine Neuron Degeneration Assay for the Validation of Potential Parkinson's Disease Genes

JoVE 835

Laura A. Berkowitz, Shusei Hamamichi, Adam L. Knight, Adam J. Harrington, Guy A. Caldwell, Kim A. Caldwell

Department of Biological Sciences, University of Alabama

Date Published: July 18, 2008 | doi:10.3791/835 | Section: JoVE General

Keywords: Neuroscience, C. elegans, Parkinson's disease, neuroprotection, alpha-synuclein, Translational Research

Chromatin Immunoprecipitation from Human Embryonic Stem Cells

JoVE 780

Stephane Bertani, Alice Kan, Frank Sauer

Department of Biochemistry, University of California - Riverside

Date Published: July 22, 2008 | doi:10.3791/780 | Section: JoVE General

Keywords: Cellular Biology, chromatin purification, chromatin immunoprecipitation, magnetic beads, vertebrates

Neutrophil Isolation Protocol

JoVE 745

Hana Oh, Brian Siano, Scott Diamond

Institute for Medicine and Engineering, University of Pennsylvania

Date Published: July 23, 2008 | doi:10.3791/745 | Section: JoVE General

Keywords: immunology, issue 17, blood, neutrophils, neutrophil polymorphonuclear granulocytes, cell separation, cell isolation

In vitro Differentiation of Mouse Embryonic Stem (mES) Cells Using the Hanging Drop Method

JoVE 825

Xiang Wang, Phillip Yang

Division of Cardiovascular Medicine, Stanford University

Date Published: July 23, 2008 | doi:10.3791/825 | Section: JoVE General

Keywords: Cell Biology, Embryonic stem cell, hanging drop, embryoid body, cardiomyocyte

Culture of myeloid dendritic cells from bone marrow precursors

JoVE 769

Jeanette Boudreau^1,2, Sandeep Koshy^2,3, Derek Cummings², Yonghong Wan²

¹Medical Sciences Program, McMaster University, ²Centre for Gene Therapeutics, McMaster University, ³Department of Chemical Engineering, University of Waterloo

Date Published: July 25, 2008 | doi:10.3791/769 | Section: JoVE General

Keywords: Immunology, dendritic cells, GM-CSF, culture, bone marrow

AC Electrokinetic Phenomena Generated by Microelectrode Structures

JoVE 813

Robert Hart¹, Jonghyun Oh², Jorge Capurro², Hongseok (Moses) Noh²

¹Biomedical Engineering, Science and Health Systems, Drexel University, ²Mechanical Engineering and Mechanics, Drexel University

Date Published: July 28, 2008 | doi:10.3791/813 | Section: JoVE General

Keywords: Bioengineering, AC Electrokinetics, AC Electroosmosis, Dielectrophoresis, Electrothermal Effect, Microelectrode, Microfluidics, Simulation, Microsphere, Microfabrication

Major Components of the Light Microscope

JoVE 843

Victoria Centonze Frohlich

Core Optical Imaging Facility, University of Texas Health Science Center at San Antonio (UTHSCSA)

Date Published: July 30, 2008 | doi:10.3791/843 | Section: JoVE General

Keywords: Basic Protocols, Current Protocols Wiley, Microscopy, Objectives, Condenser, Eyepiece