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Table of Contents

Table of Contents: Issue 68, October 2012

Jump to:  JoVE Editorial (1) JoVE General (14) JoVE Neuroscience (13) JoVE Immunology and Infection (7) JoVE Clinical and Translational Medicine (10) JoVE Bioengineering (6) JoVE Applied Physics (4) JoVE Errata (3)
 JoVE General

Tangential Flow Ultrafiltration: A “Green” Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles


JoVE 4167

1Department of Chemistry, Wright State University, 2Department of Neuroscience, Cell Biology, and Physiology, Wright State University

Date Published: October 4, 2012  |  doi:10.3791/4167  |  Section: JoVE General

 JoVE General

Cell Specific Analysis of Arabidopsis Leaves Using Fluorescence Activated Cell Sorting


JoVE 4214

1School of Life Sciences, University of Warwick, 2Warwick Systems Biology, University of Warwick

Date Published: October 4, 2012  |  doi:10.3791/4214  |  Section: JoVE General

 JoVE General

Examining BCL-2 Family Function with Large Unilamellar Vesicles


JoVE 4291

Department of Oncological Sciences, Department of Dermatology, The Tisch Cancer Institute, The Graduate School of Biological Sciences, Mount Sinai School of Medicine

Date Published: October 5, 2012  |  doi:10.3791/4291  |  Section: JoVE General

 JoVE General

Selective Capture of 5-hydroxymethylcytosine from Genomic DNA


JoVE 4441

1Department of Human Genetics, Emory University School of Medicine, 2Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago

Date Published: October 5, 2012  |  doi:10.3791/4441  |  Section: JoVE General

 JoVE General

Analysis of Single-cell Gene Transcription by RNA Fluorescent In Situ Hybridization (FISH)


JoVE 4073

1Centre for Medical Parasitology, Department of International Health, Immunology & Microbiology, Faculty of Health Sciences, University of Copenhagen, 2Department of Infectious Diseases, Copenhagen University Hospital (Rigshospitalet), 3Institute of Infection and Immunology Research, School of Biology, University of Edinburgh

Date Published: October 7, 2012  |  doi:10.3791/4073  |  Section: JoVE General

 JoVE General

Fabrication and Use of MicroEnvironment microArrays (MEArrays)


JoVE 4152

1Life Science Division, Lawrence Berkeley National Laboratory, 2Department of Comparative Biochemistry, University of California, Berkeley

Date Published: October 11, 2012  |  doi:10.3791/4152  |  Section: JoVE General

 JoVE General

Use of LysoTracker to Detect Programmed Cell Death in Embryos and Differentiating Embryonic Stem Cells


JoVE 4254

Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, Keck School of Medicine, University of Southern California

Date Published: October 11, 2012  |  doi:10.3791/4254  |  Section: JoVE General

 JoVE General

Preparation of Cell-lines for Conditional Knockdown of Gene Expression and Measurement of the Knockdown Effects on E4orf4-Induced Cell Death


JoVE 4442

Department of Molecular Microbiology, Faculty of Medicine, Technion - Israel Institute of Technology

Date Published: October 21, 2012  |  doi:10.3791/4442  |  Section: JoVE General

 JoVE General

A High-content Imaging Workflow to Study Grb2 Signaling Complexes by Expression Cloning


JoVE 4382

1MRC LMCB, University College London, 2Center for Computational and Integrative Biology, Massachusetts General Hospital

Date Published: October 30, 2012  |  doi:10.3791/4382  |  Section: JoVE General

 JoVE General

Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector


JoVE 4327

1Center for Regenerative Medicine (CReM), Boston University School of Medicine, 2Department of Hematology, Children's Hospital of Philadelphia, 3Center for Cellular and Molecular Therapeutics, Children's Hospital of Philadelphia

Date Published: October 31, 2012  |  doi:10.3791/4327  |  Section: JoVE General

 JoVE Neuroscience

Expansion of Embryonic and Adult Neural Stem Cells by In Utero Electroporation or Viral Stereotaxic Injection


JoVE 4093

DFG - Research Center and Cluster of Excellence for Regenerative Therapies Dresden, Germany

Date Published: October 6, 2012  |  doi:10.3791/4093  |  Section: JoVE Neuroscience

 JoVE Neuroscience

A Galvanotaxis Assay for Analysis of Neural Precursor Cell Migration Kinetics in an Externally Applied Direct Current Electric Field


JoVE 4193

1Institute for Biomaterials and Biomedical Engineering, University of Toronto, 2Lyndhurst Centre, Toronto Rehabilitation Institute, 3Department of Surgery, University of Toronto

Date Published: October 13, 2012  |  doi:10.3791/4193  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Imaging Analysis of Neuron to Glia Interaction in Microfluidic Culture Platform (MCP)-based Neuronal Axon and Glia Co-culture System


JoVE 4448

1Department of Neuroscience, Tufts University, 2Neuroscience Program, Tufts Sackler School of Graduate Biomedical Sciences

Date Published: October 14, 2012  |  doi:10.3791/4448  |  Section: JoVE Neuroscience

 JoVE Neuroscience

In ovo Electroporation of miRNA-based Plasmids in the Developing Neural Tube and Assessment of Phenotypes by DiI Injection in Open-book Preparations


JoVE 4384

Institute of Molecular Life Sciences, University of Zurich

Date Published: October 16, 2012  |  doi:10.3791/4384  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Patch Clamp Recordings in Inner Ear Hair Cells Isolated from Zebrafish


JoVE 4281

Natural Science Division, Pepperdine University

Date Published: October 17, 2012  |  doi:10.3791/4281  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Mapping Cortical Dynamics Using Simultaneous MEG/EEG and Anatomically-constrained Minimum-norm Estimates: an Auditory Attention Example


JoVE 4262

Department of Speech & Hearing Sciences, Institute for Learning and Brain Sciences, University of Washington

Date Published: October 24, 2012  |  doi:10.3791/4262  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Fiber-optic Implantation for Chronic Optogenetic Stimulation of Brain Tissue


JoVE 50004

1Department of Molecular & Human Genetics, Baylor College of Medicine (BCM), 2Department of Neuroscience, Baylor College of Medicine (BCM), 3Jan and Dan Duncan Neurological Research Institute, Texas Children's Hospital

Date Published: October 29, 2012  |  doi:10.3791/50004  |  Section: JoVE Neuroscience

 JoVE Immunology and Infection

Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation


JoVE 4208

Center for Inflammatory Bowel Diseases, Division of Digestive Diseases, David Geffen School of Medicine, The University of California Los Angeles, Los Angeles

Date Published: October 1, 2012  |  doi:10.3791/4208  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Visualization of Bacterial Toxin Induced Responses Using Live Cell Fluorescence Microscopy


JoVE 4227

1Department of Immunology, University of Pittsburgh School of Medicine, 2Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine

Date Published: October 1, 2012  |  doi:10.3791/4227  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

TransFLP — A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination


JoVE 3761

Global Health Institute, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL)

Date Published: October 8, 2012  |  doi:10.3791/3761  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Examination of Thymic Positive and Negative Selection by Flow Cytometry


JoVE 4269

Department of Medical Microbiology and Immunology, University of Alberta

Date Published: October 8, 2012  |  doi:10.3791/4269  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Granulocyte-dependent Autoantibody-induced Skin Blistering


JoVE 4250

1Department of Dermatology, University of Freiburg, 2Kepler High School Freiburg, 3Centre for Biological Signalling Studies (BIOSS), University of Freiburg

Date Published: October 12, 2012  |  doi:10.3791/4250  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models


JoVE 3925

1Department of Medicine, Vanderbilt University School of Medicine, 2Departments of Medicine and Immunology, Roswell Park Cancer Institute, 3Department of Medicine, University at Buffalo School of Medicine

Date Published: October 22, 2012  |  doi:10.3791/3925  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells


JoVE 4420

Center for Immunology and Inflammatory Diseases, and Pulmonary and Critical Care Unit, Massachusetts General Hospital and Harvard Medical School

Date Published: October 22, 2012  |  doi:10.3791/4420  |  Section: JoVE Immunology and Infection

 JoVE Clinical and Translational Medicine

Isolation of Soluble and Insoluble PrP Oligomers in the Normal Human Brain


JoVE 3788

1Department of Pathology, National Prion Disease Pathology Surveillance Center, Case Western Reserve University School of Medicine, 2Department of Neurology, National Prion Disease Pathology Surveillance Center, Case Western Reserve University School of Medicine

Date Published: October 3, 2012  |  doi:10.3791/3788  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

The Use of Primary Human Fibroblasts for Monitoring Mitochondrial Phenotypes in the Field of Parkinson's Disease


JoVE 4228

1German Center for Neurodegenerative Diseases, DZNE, 2Laboratory of Functional Neurogenomics, Department of Neurodegenerative Diseases, Hertie Institute for Clinical Brain Research, University of Tübingen

Date Published: October 3, 2012  |  doi:10.3791/4228  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Biomarkers in an Animal Model for Revealing Neural, Hematologic, and Behavioral Correlates of PTSD


JoVE 3361

1Department of Psychiatry, Center for the Study of Traumatic Stress, Uniformed Services University of the Health Sciences, Bethesda, Maryland, 2Department of Gene and Protein Biomarkers, GenProMarkers, Inc.

Date Published: October 10, 2012  |  doi:10.3791/3361  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Neo-Islet Formation in Liver of Diabetic Mice by Helper-dependent Adenoviral Vector-Mediated Gene Transfer


JoVE 4321

1Department of Medicine, Baylor College of Medicine, 2Division of Diabetes, Endocrinology & Metabolism, Diabetes & Endocrinology Research Center, Baylor College of Medicine, 3Department of Molecular & Cellular Biology, Baylor College of Medicine

Date Published: October 10, 2012  |  doi:10.3791/4321  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Rapid Point-of-Care Assay of Enoxaparin Anticoagulant Efficacy in Whole Blood


JoVE 3852

1Department of Pharmacology, New York Medical College, 2Department of Anesthesiology, New York Medical College

Date Published: October 12, 2012  |  doi:10.3791/3852  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Collecting And Measuring Wound Exudate Biochemical Mediators In Surgical Wounds


JoVE 50133

Department of Anesthesia, Stanford University School of Medicine

Date Published: October 20, 2012  |  doi:10.3791/50133  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

3-Dimensional Resin Casting and Imaging of Mouse Portal Vein or Intrahepatic Bile Duct System


JoVE 4272

1Department of Cell and Developmental Biology, Center for Stem Cell Biology, Vanderbilt University, 2Division of Gastroenterology, Hepatology, and Nutrition, Cincinnati Children's Hospital, 3Department of Biology, Duke University

Date Published: October 25, 2012  |  doi:10.3791/4272  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Repair of a Critical-sized Calvarial Defect Model Using Adipose-derived Stromal Cells Harvested from Lipoaspirate


JoVE 4221

1Department of Surgery, Stanford University, 2Department of Surgery, Duke University, 3Department of Surgery, Saint Joseph Mercy Hospital, 4School of Medicine, University of California, San Francisco, 5School of Dentistry, University of California, Los Angeles

Date Published: October 31, 2012  |  doi:10.3791/4221  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

High Throughput Sequential ELISA for Validation of Biomarkers of Acute Graft-Versus-Host Disease


JoVE 4247

Pediatric Blood and Marrow Transplant Program, University of Michigan

Date Published: October 31, 2012  |  doi:10.3791/4247  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

A Novel Surgical Approach for Intratracheal Administration of Bioactive Agents in a Fetal Mouse Model


JoVE 4219

1Molecular Virology and Gene Therapy, KU Leuven, 2Department of Woman and Child, KU Leuven, 3Neurobiology and Gene Therapy, KU Leuven, 4Division of Nuclear Medicine, KU Leuven, 5Biomedical NMR Unit/ MoSAIC, KU Leuven

Date Published: October 31, 2012  |  doi:10.3791/4219  |  Section: JoVE Clinical and Translational Medicine

 JoVE Bioengineering

Nanotopology of Cell Adhesion upon Variable-Angle Total Internal Reflection Fluorescence Microscopy (VA-TIRFM)


JoVE 4133

Hochschule Aalen, Institut für Angewandte Forschung

Date Published: October 2, 2012  |  doi:10.3791/4133  |  Section: JoVE Bioengineering

 JoVE Bioengineering

A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments


JoVE 4182

1Department of Biotechnology, Delft University of Technology, 2Delft Center for Systems and Control, Delft University of Technology

Date Published: October 2, 2012  |  doi:10.3791/4182  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Skin Tattooing As A Novel Approach For DNA Vaccine Delivery


JoVE 50032

1Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, 2Department of Pathology, New York University School of Medicine, 3Healthcare System, Veterans Affairs New York Harbor

Date Published: October 18, 2012  |  doi:10.3791/50032  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Fabrication and Application of Rose Bengal-chitosan Films in Laser Tissue Repair


JoVE 4158

1Bioelectronics and Neuroscience (BENS) research group, University of Western Sydney, NSW Australia, 2Australian School of Advanced Medicine, Macquarie University, NSW Australia, 3School of Medicine, University of Siena, Italy

Date Published: October 23, 2012  |  doi:10.3791/4158  |  Section: JoVE Bioengineering

 JoVE Applied Physics

Compact Quantum Dots for Single-molecule Imaging


JoVE 4236

1Department of Biomedical Engineering, Emory University, 2Department of Chemistry, Georgia Institute of Technology

Date Published: October 9, 2012  |  doi:10.3791/4236  |  Section: JoVE Applied Physics

 JoVE Applied Physics

Angle-resolved Photoemission Spectroscopy At Ultra-low Temperatures


JoVE 50129

1Institute for Solid State Research, IFW-Dresden, 2Institute of Metal Physics of National Academy of Sciences of Ukraine, 3Diamond Light Source LTD, 4Department of Physics, University of Johannesburg, 5CNR-SPIN, and Dipartimento di Fisica "E. R. Caianiello", Università di Salerno, 6Institute of Physics of Complex Matter, École Polytechnique Fédérale de Lausanne

Date Published: October 9, 2012  |  doi:10.3791/50129  |  Section: JoVE Applied Physics

 JoVE Applied Physics

Molecular Beam Mass Spectrometry With Tunable Vacuum Ultraviolet (VUV) Synchrotron Radiation


JoVE 50164

Chemical Sciences Division, Lawrence Berkeley National Laboratory

Date Published: October 30, 2012  |  doi:10.3791/50164  |  Section: JoVE Applied Physics

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