Table of Contents: Issue 35, January 2010

The 2009 Lindau Nobel Laureate Meeting: Roger Y. Tsien, Chemistry 2008

JoVE 1575

Roger Y. Tsien

Date Published: January 13, 2010  |  doi:10.3791/1575  |  Section: JoVE Editorial

Keywords: Cellular Biology, GFP, Green Fluorescent Protein, IFPs, jellyfish, PKA, Calmodulin

Presynaptically Silent Synapses Studied with Light Microscopy

JoVE 1676

Krista L. Moulder¹, Xiaoping Jiang¹, Amanda A. Taylor¹, Ann M. Benz¹, Steven Mennerick^1,2,3

¹Department of Psychiatry, Washington University School of Medicine, ²Department of Anatomy, Washington University School of Medicine, ³Department of Neurobiology, Washington University School of Medicine

Date Published: January 4, 2010  |  doi:10.3791/1676  |  Section: JoVE General

Keywords: Neurobiology, glutamate, synaptic plasticity, cAMP, excitotoxicity, homeostasis, FM1-43, presynaptic plasticity

Single-cell Suction Recordings from Mouse Cone Photoreceptors

JoVE 1681

Jin-Shan Wang, Vladimir J Kefalov

Department of Ophthalmology and Visual Sciences, Washington University in St. Louis, School of Medicine

Date Published: January 5, 2010  |  doi:10.3791/1681  |  Section: JoVE General

Keywords: Cellular Biology, mouse, cone photoreceptor, electrophysiology, suction-recording, CNG channels, retina, murine, IS-in

Fabrication and Operation of an Oxygen Insert for Adherent Cellular Cultures

JoVE 1695

Shawn Oppegard, Elly Sinkala, David Eddington

Bioengineering, University of Illinois

Date Published: January 6, 2010  |  doi:10.3791/1695  |  Section: JoVE General

Keywords: Cellular Biology, hypoxia, cell, culture, control, wound, healing, oxygen, microfluidic device, bioengineering

Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency

JoVE 1662

Adam M. McCoy, Michelle L. Collins, Luis A. Ugozzoli

Gene Expression Division, Bio-Rad Laboratories, Inc.

Date Published: January 7, 2010  |  doi:10.3791/1662  |  Section: JoVE General

Keywords: Cellular Biology, Primary cell electroporation, MEF, Bio-Rad, Gene Pulser MXcell, transfection, GFP

Undecalcified Bone Preparation for Histology, Histomorphometry and Fluorochrome Analysis

JoVE 1707

Tony Goldschlager¹, Amany Abdelkader¹, Jeffrey Kerr², Ian Boundy², Graham Jenkin¹

¹Monash Immunology and Stem Cell Laboratories, Monash University, ²Anatomy and Developmental Biology, Monash University

Date Published: January 8, 2010  |  doi:10.3791/1707  |  Section: JoVE General

Keywords: Medicine, Immunology, Bone, Histology, Undecalcified, Methylmethacrylate, Fluorochrome

In vivo-like Organotypic Murine Retinal Wholemount Culture

JoVE 1634

Sebastian Gustmann, Nicole Dünker

Institute for Anatomy, Department of Neuroanatomy, University of Duisburg-Essen

Date Published: January 11, 2010  |  doi:10.3791/1634  |  Section: JoVE General

Keywords: Neuroscience, eye, retina, organotypic culture, wholemount, dissociation, cytospin

A Protocol for the Production of KLRG1 Tetramer

JoVE 1701

Stephanie C. Terrizzi, Cindy Banh, Laurent Brossay

Department of Molecular Microbiology and Immunology, Brown University

Date Published: January 12, 2010  |  doi:10.3791/1701  |  Section: JoVE General

Keywords: Microbiology, Immunology, Basic Protocols, Tetramer, Inclusion Bodies, Refolding, Monomer, Flow Cytometry, KLRG1, Cadherins

Visualizing RNA Localization in Xenopus Oocytes

JoVE 1704

James A. Gagnon, Kimberly L. Mowry

Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University

Date Published: January 14, 2010  |  doi:10.3791/1704  |  Section: JoVE General

Keywords: Developmental Biology, RNA, Developmental Biology, Microinjection, RNA Localization, Xenopus, oocytes, VLE

Murine Model for Parkinson's Disease: from 6-OH Dopamine Lesion to Behavioral Test

JoVE 1376

Fabio S.L. da Conceição, Stacie Ngo-Abdalla, Jean-Christophe Houzel, Stevens K. Rehen

Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Brasil

Date Published: January 15, 2010  |  doi:10.3791/1376  |  Section: JoVE General

Keywords: Neuroscience, neurodegenerative disease, mice, cell therapy, model

Spinal Cord Electrophysiology

JoVE 1660

Allyn Meyer¹, Benjamin W. Gallarda^1,2, Samuel Pfaff¹, William Alaynick¹

¹The Salk Institute for Biological Studies, Howard Hughes Medical Institute and Gene Expression Laboratory, ²Biology Graduate Program, University of California San Diego - UCSD

Date Published: January 18, 2010  |  doi:10.3791/1660  |  Section: JoVE General

Keywords: Neuroscience, Electrophysiology, central pattern generator, spinal cord, artificial cerebrospinal fluid

Murine Colitis Modeling using Dextran Sulfate Sodium (DSS)

JoVE 1652

Caitlyn G. Whittem¹, Amanda D. Williams², Christopher S. Williams²

¹Department of Cancer Biology, Vanderbilt University, ²Departments of Medicine and Cancer Biology, Vanderbilt University

Date Published: January 19, 2010  |  doi:10.3791/1652  |  Section: JoVE General

Keywords: Medicine, Dextran sulfate sodium (DSS), murine acute colitis model, colon, Swiss roll, acute colonic damage

Heterotopic and Orthotopic Tracheal Transplantation in Mice used as Models to Study the Development of Obliterative Airway Disease

JoVE 1437

Xiaoqin Hua¹, Tobias Deuse^1,2, Karis R. Tang-Quan^1,2,3, Robert C. Robbins³, Hermann Reichenspurner^1,2, Sonja Schrepfer^1,2,3

¹Transplant and Stem Cell Immunobiology Lab (TSI), University Heart Center Hamburg, ²CVRC, University Hospital Hamburg, ³Department of CT Surgery, Stanford University School of Medicine

Date Published: January 20, 2010  |  doi:10.3791/1437  |  Section: JoVE General

Keywords: Immunology, orthotopic tracheal transplantation, heterotopic tracheal transplantation, obliterative airway disease, mice, luminal obliteration, histology

Surgical Induction of Endolymphatic Hydrops by Obliteration of the Endolymphatic Duct

JoVE 1728

Cliff A. Megerian, Chris Heddon, Sami Melki, Suhael Momin, Janis Paulsey, Joy Obokhare, Kumar Alagramam

Otolaryngology - Head and Neck Surgery, Case Western Reserve University

Date Published: January 22, 2010  |  doi:10.3791/1728  |  Section: JoVE General

Keywords: Medicine, Guinea Pig, Endolymphatic hydrops, Meniere's disease, surgical induction, endolymphatic duct

Right Hemihepatectomy by Suprahilar Intrahepatic Transection of the Right Hemipedicle using a Vascular Stapler

JoVE 1750

Ingmar Königsrainer, Silvio Nadalin, Alfred Königsrainer

Department of General, Visceral, and Transplant Surgery, Tübingen University Hospital

Date Published: January 25, 2010  |  doi:10.3791/1750  |  Section: JoVE General

Keywords: Medicine, Liver resection, liver tumour, intrahepatic hilus stapling, right hemipedicle

A Multi-Parametric Islet Perifusion System within a Microfluidic Perifusion Device

JoVE 1649

Adeola F. Adewola¹, Yong Wang¹, Tricia Harvat¹, David T. Eddington², Dongyoung Lee¹, Jose Oberholzer^1,2

¹Department of Surgery, University of Illinois, Chicago, ²Department of Bioengineering, University of Illinois, Chicago

Date Published: January 26, 2010  |  doi:10.3791/1649  |  Section: JoVE General

Keywords: Cellular Biology, Microfluidics, Islet perifusion, glucose ramp, imaging, perifusion, beta cells, insulin secretion

Scalable Fluidic Injector Arrays for Viral Targeting of Intact 3-D Brain Circuits

JoVE 1489

Stephanie Chan, Jacob Bernstein, Edward Boyden

Biological Engineering, Brain and Cognitive Sciences, and McGovern Institute, Massachusetts Institute of Technology

Date Published: January 21, 2010  |  doi:10.3791/1489  |  Section: JoVE Neuroscience

Keywords: JoVE Neuroscience, Lentivirus, adeno-associated virus, channelrhodopsin, optogenetics, neuroscience, infusion, transgenic, gene therapy, drug delivery, neurotechnology, brain circuits

Isolation of Mouse Peritoneal Cavity Cells

JoVE 1488

Avijit Ray, Bonnie N. Dittel

BloodCenter of Wisconsin, Blood Research Institute

Date Published: January 28, 2010  |  doi:10.3791/1488  |  Section: JoVE Immunology and Infection

Keywords: JoVE Immunology, Immune cells, Peritoneal cavity, Macrophage, B cell, B1 cell, isolation procedure

Determining the Reactivity and Titre of Serum using a Haemagglutination Assay

JoVE 1752

Maurizio Costabile

School of Pharmacy and Medical Sciences, University of South Australia

Date Published: January 29, 2010  |  doi:10.3791/1752  |  Section: JoVE Immunology and Infection

Keywords: JoVE Immunology, Haemagglutination, Titre, Reactivity, Ag-Ab complex

An Experimental Paradigm for the Prediction of Post-Operative Pain (PPOP)

JoVE 1671

Ruth Landau, John C. Kraft, Lisa Y. Flint, Brendan Carvalho, Philippe Richebé, Monica Cardoso, Patricia Lavand'homme, Michal Granot, David Yarnitsky, Alex Cahana

Department of Anesthesiology and Pain Medicine, University of Washington School of Medicine

Date Published: January 27, 2010  |  doi:10.3791/1671  |  Section: JoVE Clinical and Translational Medicine

Keywords: JoVE Medicine, diffuse noxious inhibitory control, DNIC, temporal summation, TS, psychophysical testing, endogenous analgesia, pain modulation, pregnancy-induced analgesia, cesarean section, post-operative pain, prediction

Erratum: Labeling and Imaging Cells in the Zebrafish Hindbrain

Pradeepa Jayachandran¹, Elim Hong², Rachel Brewster¹

¹Department of Biological Sciences, University of Maryland, ²Center for Neuroscience, Children's National Medical Center

Date Published: September 16, 2010  |  doi:10.3791/2556

Keywords: Errata,