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Table of Contents

Table of Contents: Issue 67, September 2012

Jump to:  JoVE Editorial (1) JoVE General (21) JoVE Neuroscience (7) JoVE Immunology and Infection (5) JoVE Clinical and Translational Medicine (9) JoVE Bioengineering (5) JoVE Applied Physics (3)
 JoVE General

Use of a Robot for High-throughput Crystallization of Membrane Proteins in Lipidic Mesophases


JoVE 4000

Membrane Structural and Functional Biology Group, Schools of Medicine and Biochemistry & Immunology, Trinity College Dublin

Date Published: September 1, 2012  |  doi:10.3791/4000  |  Section: JoVE General

 JoVE General

Harvesting and Cryo-cooling Crystals of Membrane Proteins Grown in Lipidic Mesophases for Structure Determination by Macromolecular Crystallography


JoVE 4001

Membrane Structural and Functional Biology Group, Schools of Medicine and Biochemistry & Immunology, Trinity College Dublin

Date Published: September 2, 2012  |  doi:10.3791/4001  |  Section: JoVE General

 JoVE General

Multi-parameter Measurement of the Permeability Transition Pore Opening in Isolated Mouse Heart Mitochondria


JoVE 4131

Department of Anesthesiology & Pain Medicine, Mitochondria and Metabolism Center, University of Washington, Seattle

Date Published: September 7, 2012  |  doi:10.3791/4131  |  Section: JoVE General

 JoVE General

Mouse Islet of Langerhans Isolation using a Combination of Purified Collagenase and Neutral Protease


JoVE 4137

1Department of Pediatrics and the Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, 2VITACYTE, LLC, 3Department of Medicine, Indiana University School of Medicine, 4Department of Cellular and Integrative Physiology, Indiana University School of Medicine

Date Published: September 7, 2012  |  doi:10.3791/4137  |  Section: JoVE General

 JoVE General

Procedure for Fabricating Biofunctional Nanofibers


JoVE 4135

1Department of Chemistry, Clark Atlanta University, 2Department of Physics, Clark Atlanta University, 3Department of Chemistry and Chemical Biology, Cornell University

Date Published: September 10, 2012  |  doi:10.3791/4135  |  Section: JoVE General

 JoVE General

Extraction of Tissue Antigens for Functional Assays


JoVE 4230

1Immunology and Diabetes Unit, St. Vincent's Institute of Medical Research, 2Department of Medicine, University of Melbourne

Date Published: September 10, 2012  |  doi:10.3791/4230  |  Section: JoVE General

 JoVE General

Mapping the After-effects of Theta Burst Stimulation on the Human Auditory Cortex with Functional Imaging


JoVE 3985

Montreal Neurological Institute and International laboratory for Brain, Music, and Sound (BRAMS), McGill University

Date Published: September 12, 2012  |  doi:10.3791/3985  |  Section: JoVE General

 JoVE General

Exploring Arterial Smooth Muscle Kv7 Potassium Channel Function using Patch Clamp Electrophysiology and Pressure Myography


JoVE 4263

Department of Molecular Pharmacology & Therapeutics, Loyola University Chicago

Date Published: September 14, 2012  |  doi:10.3791/4263  |  Section: JoVE General

 JoVE General

Performing Vaginal Lavage, Crystal Violet Staining, and Vaginal Cytological Evaluation for Mouse Estrous Cycle Staging Identification


JoVE 4389

1Department of Biochemistry, Microbiology and Immunology, Neural Regeneration Laboratory and Ottawa Institute of Systems Biology, 2Department of Cellular and Molecular Medicine, University of Ottawa, 3CIHR Program in Neurodegenerative Lipidomics, University of Ottawa, 4Carleton Immersive Media Studio, Azrieli School of Architecture and Urbanism

Date Published: September 15, 2012  |  doi:10.3791/4389  |  Section: JoVE General

 JoVE General

Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations


JoVE 4239

Department of Hematology, Oncology and Stem Cell Transplantation, University Medical Center Freiburg

Date Published: September 17, 2012  |  doi:10.3791/4239  |  Section: JoVE General

 JoVE General

Using Unfixed, Frozen Tissues to Study Natural Mucin Distribution


JoVE 3928

1Department of Cellular and Molecular Medicine, University of California, San Diego, 2Biosecurity and Public Health, Los Alamos National Laboratory

Date Published: September 21, 2012  |  doi:10.3791/3928  |  Section: JoVE General

 JoVE General

Flow Cytometry-based Purification of S. cerevisiae Zygotes


JoVE 4197

1Department of Pathology, Case Western Reserve University School of Medicine, 2Cell Biology Program, Case Western Reserve University School of Medicine, 3Case Comprehensive Cancer Center, Case Western Reserve University School of Medicine

Date Published: September 21, 2012  |  doi:10.3791/4197  |  Section: JoVE General

 JoVE General

Genotyping of Plant and Animal Samples without Prior DNA Purification


JoVE 3844

Thermo Scientific Molecular Biology Products, Thermo Fisher Scientific

Date Published: September 24, 2012  |  doi:10.3791/3844  |  Section: JoVE General

 JoVE General

A Protocol for the Identification of Protein-protein Interactions Based on 15N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation


JoVE 4083

1Max Planck Institute of Molecular Plant Physiology, 2University of Kaiserslautern

Date Published: September 24, 2012  |  doi:10.3791/4083  |  Section: JoVE General

 JoVE General

Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters


JoVE 4234

Department of Biological Sciences, The University of Memphis

Date Published: September 27, 2012  |  doi:10.3791/4234  |  Section: JoVE General

 JoVE General

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins


JoVE 4251

1Department of Radiation Oncology, Virginia Commonwealth University, 2Department of Biochemistry & Molecular Biology, Virginia Commonwealth University, 3Department of Anatomy & Neurobiology, Virginia Commonwealth University, 4Massey Cancer Center, Virginia Commonwealth University

Date Published: September 28, 2012  |  doi:10.3791/4251  |  Section: JoVE General

 JoVE General

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip


JoVE 3851

1Molecular Microbiology and Immunology, University of Missouri, 2Department of Surgery, University of Missouri, 3Child Health, University of Missouri

Date Published: September 29, 2012  |  doi:10.3791/3851  |  Section: JoVE General

 JoVE Neuroscience

Microdialysis of Ethanol During Operant Ethanol Self-administration and Ethanol Determination by Gas Chromatography


JoVE 4142

College of Pharmacy, Division of Pharmacology and Toxicology, The University of Texas at Austin

Date Published: September 5, 2012  |  doi:10.3791/4142  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Time-lapse Imaging of Neuroblast Migration in Acute Slices of the Adult Mouse Forebrain


JoVE 4061

The Cellular Neurobiology Unit, Centre de Recherche Université Laval Robert-Giffard

Date Published: September 12, 2012  |  doi:10.3791/4061  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Preparation of Acute Subventricular Zone Slices for Calcium Imaging


JoVE 4071

Department of Neurosurgery and Cellular & Molecular Physiology, Yale University School of Medicine

Date Published: September 19, 2012  |  doi:10.3791/4071  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Measuring the Subjective Value of Risky and Ambiguous Options using Experimental Economics and Functional MRI Methods


JoVE 3724

1Section of Comparative Medicine, Yale School of Medicine, 2Department of Neurobiology, Yale School of Medicine, 3Center for Neural Science, New York University, 4Department of Psychology, New York University, 5Department of Economics, New York University

Date Published: September 19, 2012  |  doi:10.3791/3724  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Multiphoton Microscopy of Cleared Mouse Brain Expressing YFP


JoVE 3848

1Department of Biomedical Engineering, Yale University, 2Department of Biomedical Engineering, Louisiana Tech University

Date Published: September 23, 2012  |  doi:10.3791/3848  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Antibody Transfection into Neurons as a Tool to Study Disease Pathogenesis


JoVE 4154

1Research Service, Veterans Administration Medical Center, Memphis, TN, 2Department of Neurology, University of Tennessee Health Science Center, Memphis, TN, 3Department of Anatomy/Neurobiology, University of Tennessee Health Science Center, Memphis, TN

Date Published: September 26, 2012  |  doi:10.3791/4154  |  Section: JoVE Neuroscience

 JoVE Immunology and Infection

Sample Preparation of Mycobacterium tuberculosis Extracts for Nuclear Magnetic Resonance Metabolomic Studies


JoVE 3673

1School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, 2Department of Chemistry, University of Nebraska-Lincoln

Date Published: September 3, 2012  |  doi:10.3791/3673  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Isolation of Lymphocytes from Mouse Genital Tract Mucosa


JoVE 4391

1Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, 2California NanoSystems

Date Published: September 3, 2012  |  doi:10.3791/4391  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay


JoVE 3822

1Applied Bioscience Program, Faculty of Science, University of Ontario Institute of Technology, 2Nursing Program, Faculty of Health Sciences, University of Ontario Institute of Technology, 3Medical Laboratory Science Program, Faculty of Health Sciences, University of Ontario Institute of Technology

Date Published: September 9, 2012  |  doi:10.3791/3822  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay


JoVE 4302

Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center

Date Published: September 16, 2012  |  doi:10.3791/4302  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Fluorescent in situ Hybridization on Mitotic Chromosomes of Mosquitoes


JoVE 4215

Department of Entomology, Virginia Tech

Date Published: September 17, 2012  |  doi:10.3791/4215  |  Section: JoVE Immunology and Infection

 JoVE Clinical and Translational Medicine

Intraductal Injection of LPS as a Mouse Model of Mastitis: Signaling Visualized via an NF-κB Reporter Transgenic


JoVE 4030

1Cancer Biology Department, Vanderbilt University Medical Center, 2Department of Medicine, Vanderbilt University Medical Center, 3Department of Pharmaceutical Sciences, University of Hawaii at Hilo College of Pharmacy

Date Published: September 4, 2012  |  doi:10.3791/4030  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Models of Bone Metastasis


JoVE 4260

1Department of Pharmacology, Vanderbilt University, 2Vanderbilt Center for Bone Biology, Vanderbilt University, 3Department of Veterans Affairs, Tennessee Valley Healthcare System (VISN 9), 4Department of Medicine, Division of Clinical Pharmacology, Vanderbilt University, 5Department of Cancer Biology, Vanderbilt University

Date Published: September 4, 2012  |  doi:10.3791/4260  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Functional Assessment of Intestinal Motility and Gut Wall Inflammation in Rodents: Analyses in a Standardized Model of Intestinal Manipulation


JoVE 4086

Department of Surgery, University of Bonn

Date Published: September 11, 2012  |  doi:10.3791/4086  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Modeling Colitis-Associated Cancer with Azoxymethane (AOM) and Dextran Sulfate Sodium (DSS)


JoVE 4100

Division of Gastroenterology, Washington University School of Medicine

Date Published: September 11, 2012  |  doi:10.3791/4100  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Doppler Optical Coherence Tomography of Retinal Circulation


JoVE 3524

1Department of Ophthalmology, Oregon Health and Science University, 2Department of Ophthalmology, University of Southern California

Date Published: September 18, 2012  |  doi:10.3791/3524  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Driving Simulation in the Clinic: Testing Visual Exploratory Behavior in Daily Life Activities in Patients with Visual Field Defects


JoVE 4427

1Department of Neurology, Universitätsmedizin Charité, 2Center for Stroke Research Berlin (CSB), Universitätsmedizin Charité, 3Berlin School of Mind and Brain, Humboldt Universität zu Berlin

Date Published: September 18, 2012  |  doi:10.3791/4427  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Modeling Intracerebral Hemorrhage in Mice: Injection of Autologous Blood or Bacterial Collagenase


JoVE 4289

1Department of Physiology and Pharmacology, Loma Linda University School of Medicine, 2College of Natural and Agricultural Sciences, University of California, Riverside, 3Department of Anesthesiology, Loma Linda University School of Medicine, 4Department of Neurosurgery, Loma Linda University School of Medicine

Date Published: September 22, 2012  |  doi:10.3791/4289  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Stereotactic Intracranial Implantation and In vivo Bioluminescent Imaging of Tumor Xenografts in a Mouse Model System of Glioblastoma Multiforme


JoVE 4089

Department of Radiation Oncology, University of Pennsylvania

Date Published: September 25, 2012  |  doi:10.3791/4089  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Processing of Primary Brain Tumor Tissue for Stem Cell Assays and Flow Sorting


JoVE 4111

Stem Cell and Cancer Research Institute, McMaster University

Date Published: September 25, 2012  |  doi:10.3791/4111  |  Section: JoVE Clinical and Translational Medicine

 JoVE Bioengineering

Combinatorial Synthesis of and High-throughput Protein Release from Polymer Film and Nanoparticle Libraries


JoVE 3882

Department of Chemical and Biological Engineering, Iowa State University

Date Published: September 6, 2012  |  doi:10.3791/3882  |  Section: JoVE Bioengineering

 JoVE Bioengineering

In vitro Synthesis of Native, Fibrous Long Spacing and Segmental Long Spacing Collagen


JoVE 4417

1Department of Chemistry, University of Toronto, 2Institute for Optical Sciences, University of Toronto

Date Published: September 20, 2012  |  doi:10.3791/4417  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Micropipette Aspiration of Substrate-attached Cells to Estimate Cell Stiffness


JoVE 3886

1Section of Respiratory, Critical Care and Sleep Medicine, Department of Medicine, University of Illinois, 2Institute for Medicine and Engineering, University of Pennsylvania

Date Published: September 27, 2012  |  doi:10.3791/3886  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Simple Microfluidic Devices for in vivo Imaging of C. elegans, Drosophila and Zebrafish


JoVE 3780

1Neurobiology, NCBS-TIFR, 2Department of Biological Sciences, TIFR

Date Published: September 30, 2012  |  doi:10.3791/3780  |  Section: JoVE Bioengineering

 JoVE Applied Physics

Synthesis of Phase-shift Nanoemulsions with Narrow Size Distributions for Acoustic Droplet Vaporization and Bubble-enhanced Ultrasound-mediated Ablation


JoVE 4308

Department of Mechanical Engineering, Boston University

Date Published: September 13, 2012  |  doi:10.3791/4308  |  Section: JoVE Applied Physics

 JoVE Applied Physics

Probing and Mapping Electrode Surfaces in Solid Oxide Fuel Cells


JoVE 50161

1Center for Innovative Fuel Cells and Battery Technologies, School of Materials Science and Engineering, Georgia Institute of Technology, 2School of Chemistry and Biochemistry, Georgia Institute of Technology

Date Published: September 20, 2012  |  doi:10.3791/50161  |  Section: JoVE Applied Physics

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