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The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.

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Table of Contents

Table of Contents: Issue 47, January 2011

Jump to:  JoVE General (16) JoVE Neuroscience (10) JoVE Immunology and Infection (9) JoVE Clinical and Translational Medicine (12) JoVE Bioengineering (5) JoVE Errata (1)
 JoVE General

Direct Delivery of MIF Morpholinos Into the Zebrafish Otocyst by Injection and Electroporation Affects Inner Ear Development


JoVE 2466

1, 2, 2, 2,3, 2,4

1Department of Veterinary Science, University of Wisconsin, Madison, 2Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI, 3Present address: Department of Pulmonary Medicine, University of Michigan, Ann Arbor, MI, 4Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI

Date Published: January 7, 2011  |  doi:10.3791/2466  |  Section: JoVE General

Keywords: Developmental Biology, Zebrafish inner ear, microinjection, electroporation, morpholino

 JoVE General

Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes


JoVE 2269

1, 2, 2, 3

1Department of Energy, Environmental & Chemical Engineering, Washington University in St. Louis, 2Department of Biomedical Engineering, Washington University in St. Louis, 3Department of Biomedical Engineering and Cardiac Bioelectricity and Arrhythmia Center, Washington University in St. Louis

Date Published: January 10, 2011  |  doi:10.3791/2269  |  Section: JoVE General

Keywords: Cellular Biology, patch clamp, ion channel, electrophysiology, biophysics, exogenous expression system, Xenopus oocyte, mRNA, transcription

 JoVE General

The Production of C. elegans Transgenes via Recombineering with the galK Selectable Marker


JoVE 2331

*1, *2, 2, 2

1Department of Radiation Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, 2Department of Medicine, Division of Geriatric Medicine and Pittsburgh Institute for Neurodegenerative Diseases, University of Pittsburgh

Date Published: January 11, 2011  |  doi:10.3791/2331  |  Section: JoVE General

Keywords: Genetics, C. elegans, transgenes, fosmid clone, galK, recombineering, homologous recombination, E. coli

 JoVE General

Modeling Biological Membranes with Circuit Boards and Measuring Electrical Signals in Axons: Student Laboratory Exercises


JoVE 2325

1, 1, 2, 1

1Department of Biology, University of Kentucky, 2Department of Physiology, University of Toronto

Date Published: January 18, 2011  |  doi:10.3791/2325  |  Section: JoVE General

Keywords: Basic Protocols, Invertebrate, Crayfish, Modeling, Student laboratory, Nerve cord

 JoVE General

In vivo Quantification of G Protein Coupled Receptor Interactions using Spectrally Resolved Two-photon Microscopy


JoVE 2247

1, 1, 1,2

1Department of Physics, University of Wisconsin - Milwaukee, 2Department of Biological Sciences, University of Wisconsin - Milwaukee

Date Published: January 19, 2011  |  doi:10.3791/2247  |  Section: JoVE General

Keywords: Cellular Biology, Forster (Fluorescence) Resonance Energy Transfer (FRET), protein-protein interactions, protein complex, in vivo determinations, spectral resolution, two-photon microscopy, G protein-coupled receptor (GPCR), sterile 2 alpha-factor protein (Ste2p)

 JoVE Neuroscience

Growth and Differentiation of Adult Hippocampal Arctic Ground Squirrel Neural Stem Cells


JoVE 2199

1, 2, 3, 4

1Alaska Basic Neuroscience Program, Institute of Arctic Biology, University of Alaska at Fairbanks, 2Department Biochemistry, Hood College, 3Department of Cell Biology, Neuronascent, Inc., 4Research and Development, Neuronascent, Inc.

Date Published: January 7, 2011  |  doi:10.3791/2199  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Arctic ground squirrels, ischemia, neurogenesis, hibernation, tolerance, neuron

 JoVE Neuroscience

Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay


JoVE 2457

1,2, 2, 2, 2, 2

1Department of Anatomical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran, 2Department of Neurosurgery, The University of Florida

Date Published: January 11, 2011  |  doi:10.3791/2457  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Embryonic Neural Stem Cells, Neurosphere Assay, Isolation, Expansion

 JoVE Neuroscience

High-density EEG Recordings of the Freely Moving Mice using Polyimide-based Microelectrode


JoVE 2562

1,2, 1,2, 1,2, 3, 1,2

1Center for Neural Science , Korea Institute of Science and Technology (KIST), 2Department of Neuroscience, University of Science and Technology, 3Fab Service Department, Korea Advanced Nano Fab Center

Date Published: January 11, 2011  |  doi:10.3791/2562  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Electroencephalography (EEG), Mouse, Microelectrode, Brain Imaging

 JoVE Neuroscience

Membrane Potentials, Synaptic Responses, Neuronal Circuitry, Neuromodulation and Muscle Histology Using the Crayfish: Student Laboratory Exercises


JoVE 2322

*1, *1, *2, *1

1Department of Biology, University of Kentucky, 2Department of Physiology, University of Toronto

Date Published: January 18, 2011  |  doi:10.3791/2322  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Invertebrate, Crayfish, neurophysiology, muscle, anatomy, electrophysiology

 JoVE Neuroscience

Behavioural Pharmacology in Classical Conditioning of the Proboscis Extension Response in Honeybees (Apis mellifera)


JoVE 2282

*, *, ,

Fachbereich Bio/Chem/Pharm, Institut für Biologie – Neurobiologie, Freie Universität Berlin

Date Published: January 24, 2011  |  doi:10.3791/2282  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Classical conditioning, behavioural pharmacology, insect, invertebrate, honeybee, learning, memory

 JoVE Immunology and Infection

Facilitating the Analysis of Immunological Data with Visual Analytic Techniques


JoVE 2397

1,2, 1, 3, 2,3, 1, 1

1Department of Paediatrics, Division of Infectious and Immunological Diseases, Child and Family Research Institute, University of British Columbia, 2Department of Computer Science, University of British Columbia, 3Department of Psychology, University of British Columbia

Date Published: January 2, 2011  |  doi:10.3791/2397  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Visual analytics, flow cytometry, Luminex, Tableau, cytokine, innate immunity, single nucleotide polymorphism

 JoVE Immunology and Infection

Ex vivo Expansion of Tumor-reactive T Cells by Means of Bryostatin 1/Ionomycin and the Common Gamma Chain Cytokines Formulation


JoVE 2381

1, 2, 3, 3, 1

1Department of Microbiology & Immunology, Virginia Commonwealth University- Massey Cancer Center, 2Department of Internal Medicine, Virginia Commonwealth University- Massey Cancer Center, 3Department of Surgery, Virginia Commonwealth University- Massey Cancer Center

Date Published: January 14, 2011  |  doi:10.3791/2381  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Adoptive T cell therapy, Breast Cancer, HER-2/neu, common gamma chain cytokines, Bryostatin 1, Ionomycin

 JoVE Immunology and Infection

Pseudomonas aeruginosa and Saccharomyces cerevisiae Biofilm in Flow Cells


JoVE 2383

1, 1, 1, 2

1Department of Systems Biology, Danish Technical University, 2Department of Biology, University of Copenhagen

Date Published: January 15, 2011  |  doi:10.3791/2383  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Biofilm, Pseudomonas aeruginosa, Bacteria, Yeast, Saccharomyces cerevisiae, Flow cell system, Confocal Lases Scanning Microscopy, Microbiology, FLO11, Systems biology

 JoVE Immunology and Infection

RNA Interference in Ticks


JoVE 2474

1, 1, 1,2

1Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, 2(CSIC-UCLM-JCCM), Instituto de Investigación en Recursos Cinegéticos IREC

Date Published: January 20, 2011  |  doi:10.3791/2474  |  Section: JoVE Immunology and Infection

Keywords: Infectious Disease, Ticks, RNA interference, genetics, funtional genomics, gene expression, tick-borne pathogens

 JoVE Immunology and Infection

An Analytical Tool-box for Comprehensive Biochemical, Structural and Transcriptome Evaluation of Oral Biofilms Mediated by Mutans Streptococci


JoVE 2512

1, 1,2, 3, 1,4

1Center for Oral Biology, University of Rochester Medical Center, 2State Key Laboratory of Oral Diseases, Sichuan University, 3Department of General Medicine, Glostrup Hospital, Glostrup, Denmark, 4Department of Microbiology and Immunology, University of Rochester Medical Center

Date Published: January 25, 2011  |  doi:10.3791/2512  |  Section: JoVE Immunology and Infection

Keywords: Microbiology, Extracellular matrix, polysaccharides, biofilm, mutans streptococci, glucosyltransferases, confocal fluorescence, microarray

 JoVE Clinical and Translational Medicine

Intra-Operative Behavioral Tasks in Awake Humans Undergoing Deep Brain Stimulation Surgery


JoVE 2156

*1,2, *1,2, 1,2, 1,2

1Nayef Al-Rodhan Laboratories for Cellular Neurosurgery and Neurosurgical Technology, Harvard Medical School, 2Department of Neurosurgery , Massachusetts General Hospital

Date Published: January 6, 2011  |  doi:10.3791/2156  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Intra-Operative Physiology, Cognitive Neuroscience, Behavioral Neuroscience, Subthalamic Nucleus, Single-Unit Activity, Parkinson Disease, Deep Brain Stimulation

 JoVE Clinical and Translational Medicine

Modeling Stroke in Mice - Middle Cerebral Artery Occlusion with the Filament Model


JoVE 2423

, , ,

Department for Experimental Neurology, Center for Stroke Research Berlin, Charité Universitätsmedizin

Date Published: January 6, 2011  |  doi:10.3791/2423  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Stroke, middle cerebral artery occlusion, MCAo, animal model, mouse, techniques

 JoVE Clinical and Translational Medicine

An Investigation of the Effects of Sports-related Concussion in Youth Using Functional Magnetic Resonance Imaging and the Head Impact Telemetry System


JoVE 2226

1,2,3,4,5, 1, 1, 1, 3, 6,7

1Graduate Department of Rehabilitation Science, University of Toronto, 2Occupational Science and Occupational Therapy, University of Toronto, 3Department of Psychology, University of Toronto, 4Bloorview Kids Rehab, 5Toronto Rehab, 6Cognitive Neurology, Sunnybrook Health Sciences Centre, 7Faculty of Medicine, University of Toronto

Date Published: January 12, 2011  |  doi:10.3791/2226  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Mild traumatic brain injury, concussion, fMRI, youth, Head Impact Telemetry System

 JoVE Clinical and Translational Medicine

A Novel Technique of Rescuing Capsulorhexis Radial Tear-out using a Cystotome


JoVE 2317

*1, *2, 3, 4, 1

1Department of Ophthalmology, Hairmyres Hospital, NHS Lanarkshire, 2Department of Ophthalmology, Royal Devon and Exeter NHS Foundation Trust, 3National Institute of Ophthalmology, 4Department of Ophthalmology, South Devon Healthcare NHS Trust

Date Published: January 16, 2011  |  doi:10.3791/2317  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Phacoemulsification surgery, cataract surgery, capsulorhexis, capsulotomy, technique, Continuous curvilinear capsulorhexis, cystotome, capsulorhexis radial tear, capulorhexis COMPLICATION

 JoVE Clinical and Translational Medicine

A Novel Rescue Technique for Difficult Intubation and Difficult Ventilation


JoVE 1421

1, 1, 1, 1, 2

1Department of Anesthesia, Children’s Hospital of Michigan, 2Department of Anesthesia, St. Jude Children’s Research Hospital

Date Published: January 17, 2011  |  doi:10.3791/1421  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, difficult ventilation, difficult intubation, nasal, saturation

 JoVE Clinical and Translational Medicine

Guidelines for Elective Pediatric Fiberoptic Intubation


JoVE 2364

1, 2, 2, 3

1Department of Anesthesia, St. Jude Children's Research Hospital, 2Department of Anesthesia, Children's Hospital of Michigan, 3Department of Anesthesiology, Children's Hospital of Michigan

Date Published: January 17, 2011  |  doi:10.3791/2364  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Fiberoptic, Intubation, Pediatric, elective

 JoVE Clinical and Translational Medicine

Generation of Comprehensive Thoracic Oncology Database - Tool for Translational Research


JoVE 2414

1, 2, 2, 2, 2, 2, 1, 2, 3, 2, 4, 5, 6, 2

1Pritzker School of Medicine, University of Chicago, 2Department of Medicine, University of Chicago, 3Department of Medicine, Northshore University Health Systems, 4Department of Pathology, University of Chicago, 5Department of Surgery, University of Chicago, 6Department of Biostatistics, University of Chicago

Date Published: January 22, 2011  |  doi:10.3791/2414  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Database, Thoracic oncology, Bioinformatics, Biorepository, Microsoft Access, Proteomics, Genomics

 JoVE Clinical and Translational Medicine

A Mouse Model of in Utero Transplantation


JoVE 2303

1,2, 1,2, 1,2,3, 1,2,3

1Department of Surgery, University of California, 2Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, 3Biomedical Sciences Program, University of California

Date Published: January 27, 2011  |  doi:10.3791/2303  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, development, stem cells, transplantation, in utero

 JoVE Clinical and Translational Medicine

Contrast Enhanced Vessel Imaging using MicroCT


JoVE 2377

1,2, 1,3,4

1Greehey Children's Cancer Research Institute, University of Texas Health Science Center at San Antonio, 2Small Animal Imaging Resources facility, University of Texas Health Science Center at San Antonio, 3Department of Cellular and Structural Biology, University of Texas Health Science Center at San Antonio, 4Department of Pediatrics, University of Texas Health Science Center at San Antonio

Date Published: January 27, 2011  |  doi:10.3791/2377  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, vessel imaging, eXIA 160XL, microCT, advanced visualization, 2DTF

 JoVE Clinical and Translational Medicine

Expired CO2 Measurement in Intubated or Spontaneously Breathing Patients from the Emergency Department


JoVE 2508

, ,

Acute Medicine Department Accidents and Emergency Unit, Universit Catholique de Louvain Cliniques Universitaires Saint-Luc

Date Published: January 29, 2011  |  doi:10.3791/2508  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, capnography, CO2, emergency medicine, end-tidal CO2

 JoVE Bioengineering

Tri-layered Electrospinning to Mimic Native Arterial Architecture using Polycaprolactone, Elastin, and Collagen: A Preliminary Study


JoVE 2084

1, 1, 2, 3, 1

1Department of Biomedical Engineering, Virginia Commonwealth University, 2Department of Anatomy and Neurobiology, Virginia Commonwealth University, 3Department of Cardiovascular Surgery, University Hospital of Geneva

Date Published: January 4, 2011  |  doi:10.3791/2084  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Electrospinning, Vascular Graft, Multilayer, Polycaprolactone, Elastin

 JoVE Bioengineering

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging


JoVE 2306

1, 2, 1

1Department of Bioengineering, Rice University, 2Department of Molecular and Cellular Oncology, The Univeristy of Texas M. D. Anderson Cancer Center

Date Published: January 11, 2011  |  doi:10.3791/2306  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Optical imaging, intravital microscopy, in vivo microscopy, endoscopic microscopy, fiber bundle

 JoVE Bioengineering

Electrospinning Fundamentals: Optimizing Solution and Apparatus Parameters


JoVE 2494

1, 1,2, 3, 1,3,4

1Department of Biomedical Engineering, University of Michigan, 2State Key Laboratory of Bioelectronics, Southeast University, 3Department of Neurology, University of Michigan, 4Geriatrics Research, Education and Clinical Center, Veterans Affairs Ann Arbor Healthcare Center

Date Published: January 21, 2011  |  doi:10.3791/2494  |  Section: JoVE Bioengineering

Keywords: Bioengineering, electrospinning, nanofibers, scaffold, alignment

 JoVE Bioengineering

A Microfluidic-based Hydrodynamic Trap for Single Particles


JoVE 2517

1, 2, 1,2

1Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, 2Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign

Date Published: January 21, 2011  |  doi:10.3791/2517  |  Section: JoVE Bioengineering

Keywords: Bioengineering, hydrodynamic, trap, trapping, confinement, micromanipulation, microfluidics, stagnation point flow

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