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Basic Methods in Cellular and Molecular Biology

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Model Organisms I

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Table of Contents

Table of Contents: Issue 76, June 2013

Jump to:  JoVE Editorial (2) JoVE Biology (11) JoVE Neuroscience (7) JoVE Immunology and Infection (8) JoVE Clinical and Translational Medicine (12) JoVE Bioengineering (6) JoVE Applied Physics (2) JoVE Chemistry (1) JoVE Behavior (12) JoVE Errata (1)
 JoVE Biology

Assessing Murine Resistance Artery Function Using Pressure Myography

1Anesthesia Center for Critical Care Research, Department of Anesthesia, Critical Care, and Pain Medicine, Massachusetts General Hospital, Harvard Medical School


JoVE 50328

Date Published: June 7, 2013  |  doi:10.3791/50328  |  Section: JoVE Biology

 JoVE Biology

Efficient and Rapid Isolation of Early-stage Embryos from Arabidopsis thaliana Seeds

1Institute of Plant Biology and Zürich-Basel Plant Science Center, University of Zürich


JoVE 50371

Date Published: June 7, 2013  |  doi:10.3791/50371  |  Section: JoVE Biology

 JoVE Biology

Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media

1Department of Pharmacology & Physiology, Drexel University College of Medicine


JoVE 50294

Date Published: June 14, 2013  |  doi:10.3791/50294  |  Section: JoVE Biology

 JoVE Biology

Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae

1The Lewis-Sigler Institute for Integrative Genomics, Princeton University, 2Graduate Program in Quantitative and Computational Biology, Princeton University, 3Department of Molecular Biology, Princeton University


JoVE 50382

Date Published: June 14, 2013  |  doi:10.3791/50382  |  Section: JoVE Biology

 JoVE Biology

Giant Liposome Preparation for Imaging and Patch-Clamp Electrophysiology

1Department of Physiology and Biophysics, University of Washington


JoVE 50227

Date Published: June 21, 2013  |  doi:10.3791/50227  |  Section: JoVE Biology

 JoVE Biology

Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy

1Department of Pharmacology, Rush University, 2Pulmonary and Critical Care Department, Rush University


JoVE 50316

Date Published: June 21, 2013  |  doi:10.3791/50316  |  Section: JoVE Biology

 JoVE Biology

Culturing Primary Rat Inner Medullary Collecting Duct Cells

1Anchored Signalling, Max-Delbrück-Center for Molecular Medicine, 2Leibniz Institute for Molecular Pharmacology (FMP), 3Charité University Medicine Berlin


JoVE 50366

Date Published: June 21, 2013  |  doi:10.3791/50366  |  Section: JoVE Biology

 JoVE Biology

Prostaglandin Extraction and Analysis in Caenorhabditis elegans

1Department of Pharmacology and Toxicology, University of Alabama at Birmingham, 2Department of Cell, Developmental, and Integrative Biology, University of Alabama at Birmingham


JoVE 50447

Date Published: June 25, 2013  |  doi:10.3791/50447  |  Section: JoVE Biology

 JoVE Biology

Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy

1Department of Biochemistry and Molecular Biology, State University of New York Upstate Medical University


JoVE 50448

Date Published: June 25, 2013  |  doi:10.3791/50448  |  Section: JoVE Biology

 JoVE Biology

Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs

1Department of Molecular Biology, Massachusetts General Hospital, 2Department of Genetics, Harvard Medical School


JoVE 50434

Date Published: June 27, 2013  |  doi:10.3791/50434  |  Section: JoVE Biology

 JoVE Biology

Generation of Human Cardiomyocytes: A Differentiation Protocol from Feeder-free Human Induced Pluripotent Stem Cells

1Humanitas Clinical and Research Center, Italy, 2Institute of Genetic and Biomedical Research (IRGB), National Research Council (CNR)


JoVE 50429

Date Published: June 28, 2013  |  doi:10.3791/50429  |  Section: JoVE Biology

 JoVE Neuroscience

Simultaneous Whole-cell Recordings from Photoreceptors and Second-order Neurons in an Amphibian Retinal Slice Preparation

1Department of Ophthalmology and Visual Sciences, University of Nebraska Medical Center, 2Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center


JoVE 50007

Date Published: June 1, 2013  |  doi:10.3791/50007  |  Section: JoVE Neuroscience

 JoVE Neuroscience

An Isolated Semi-intact Preparation of the Mouse Vestibular Sensory Epithelium for Electrophysiology and High-resolution Two-photon Microscopy

1Discipline of Biomedical Science, School of Medical Sciences, Sydney Medical School, University of Sydney, 2School of Biomedical Sciences and Pharmacy, University of Newcastle


JoVE 50471

Date Published: June 13, 2013  |  doi:10.3791/50471  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Trypsin Digest Protocol to Analyze the Retinal Vasculature of a Mouse Model

1Department of Ophthalmology, Northwestern University Feinberg School of Medicine


JoVE 50489

Date Published: June 13, 2013  |  doi:10.3791/50489  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Motor Nerve Transection and Time-lapse Imaging of Glial Cell Behaviors in Live Zebrafish

1Department of Biology, University of Virginia


JoVE 50621

Date Published: June 20, 2013  |  doi:10.3791/50621  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo

1Department of Biology, Washington University in St. Louis, 2Division of Biological Science, Graduate School of Science, Nagoya University, 3Department of Psychiatry, Washington University in St. Louis


JoVE 3921

Date Published: June 26, 2013  |  doi:10.3791/3921  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Improved Preparation and Preservation of Hippocampal Mouse Slices for a Very Stable and Reproducible Recording of Long-term Potentiation

1Department of Neurosciences, Research Institute for Biosciences, University of Mons


JoVE 50483

Date Published: June 26, 2013  |  doi:10.3791/50483  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Applications of EEG Neuroimaging Data: Event-related Potentials, Spectral Power, and Multiscale Entropy

1Rotman Research Institute, Baycrest


JoVE 50131

Date Published: June 27, 2013  |  doi:10.3791/50131  |  Section: JoVE Neuroscience

 JoVE Immunology and Infection

Single Cell Measurements of Vacuolar Rupture Caused by Intracellular Pathogens

1Dynamique des Interactions Hôte Pathogène, Institut Pasteur, Paris, France, 2Imagopole, Institut Pasteur, Paris, France, 3Pathogenomique Mycobacterienne Integrée, Institut Pasteur, Paris, France


JoVE 50116

Date Published: June 12, 2013  |  doi:10.3791/50116  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization

1Department of Cardiology, University of Heidelberg


JoVE 50332

Date Published: June 12, 2013  |  doi:10.3791/50332  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Use of Image Cytometry for Quantification of Pathogenic Fungi in Association with Host Cells

1Department of Biology, Merrimack College, 2Center for Biotechnology and Biomedical Sciences, Merrimack College, 3Department of Technology R&D, Nexcelom Bioscience LLC


JoVE 50599

Date Published: June 19, 2013  |  doi:10.3791/50599  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

A Tetracycline-regulated Cell Line Produces High-titer Lentiviral Vectors that Specifically Target Dendritic Cells

1Mork Family Department of Chemical Engineering and Materials Science, Viterbi School of Engineering, University of Southern California, Los Angeles


JoVE 50606

Date Published: June 19, 2013  |  doi:10.3791/50606  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Real-time Live Imaging of T-cell Signaling Complex Formation

1The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University


JoVE 50076

Date Published: June 23, 2013  |  doi:10.3791/50076  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

1Department of Animal Science, Texas A&M University, 2Department of Biology, Texas A&M University, 3Department of Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University


JoVE 50323

Date Published: June 23, 2013  |  doi:10.3791/50323  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Multi-target Parallel Processing Approach for Gene-to-structure Determination of the Influenza Polymerase PB2 Subunit

1Protein Crystallization Lab, Emerald Bio, 2Molecular Biology Lab, Emerald Bio, 3Scientific Sales Representative, Emerald Bio, 4Group Leader II, Emerald Bio, 5Group Leader I, Emerald Bio, 6Chair of Advisory Board, Emerald Bio, 7Director of Multi-Target Services, Emerald Bio, 8Senior Project Leader, Emerald Bio, 9Project Leader II & SSGCID Site Manager, Emerald Bio


JoVE 4225

Date Published: June 28, 2013  |  doi:10.3791/4225  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Peptide-based Identification of Functional Motifs and their Binding Partners

1Department of Microbiology, Biochemistry, & Immunology, Morehouse School of Medicine, 2Institute for Systems Biology, 3Advanced Medical & Dental Institute, Universiti Sains Malaysia


JoVE 50362

Date Published: June 30, 2013  |  doi:10.3791/50362  |  Section: JoVE Immunology and Infection

 JoVE Clinical and Translational Medicine

In situ Transverse Rectus Abdominis Myocutaneous Flap: A Rat Model of Myocutaneous Ischemia Reperfusion Injury

1Department of Surgery, Royal Infirmary of Edinburgh, 2Department of Nephrology, Royal Infirmary of Edinburgh


JoVE 50473

Date Published: June 8, 2013  |  doi:10.3791/50473  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Photothrombotic Ischemia: A Minimally Invasive and Reproducible Photochemical Cortical Lesion Model for Mouse Stroke Studies

1Department of Neuroscience, University of Turin, 2Neuroscience Institute of Turin (NIT), University of Turin, 3Neuroscience Institute Cavalieri-Ottolenghi (NICO), University of Turin, 4Department of Anatomy, Pharmacology and Forensic Medicine, University of Turin


JoVE 50370

Date Published: June 9, 2013  |  doi:10.3791/50370  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Coculture Analysis of Extracellular Protein Interactions Affecting Insulin Secretion by Pancreatic Beta Cells

1Pediatric Diabetes Research Center, University of California, San Diego, 2Janssen Research & Development, 3Department of Medicine, University of California, San Diego


JoVE 50365

Date Published: June 15, 2013  |  doi:10.3791/50365  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

A Novel High-resolution In vivo Imaging Technique to Study the Dynamic Response of Intracranial Structures to Tumor Growth and Therapeutics

1Brain Tumor Research Centre, Hospital for Sick Children, Toronto Medical Discovery Tower, 2Ontario Cancer Institute, Princess Margaret Hospital, 3Neurosurgery, Toronto Western Hospital


JoVE 50363

Date Published: June 16, 2013  |  doi:10.3791/50363  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

2-Vessel Occlusion/Hypotension: A Rat Model of Global Brain Ischemia

1Department of Emergency Medicine, Wayne State University School of Medicine, 2Cardiovascular Research Institute, Wayne State University School of Medicine, 3Department of Physiology, Wayne State University School of Medicine


JoVE 50173

Date Published: June 22, 2013  |  doi:10.3791/50173  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Formation of Human Prostate Epithelium Using Tissue Recombination of Rodent Urogenital Sinus Mesenchyme and Human Stem Cells

1Department of Surgery, Section of Urology, University of Chicago, 2Committee on Cancer Biology, University of Chicago


JoVE 50327

Date Published: June 22, 2013  |  doi:10.3791/50327  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Deep Neuromuscular Blockade Leads to a Larger Intraabdominal Volume During Laparoscopy

1Department of Anesthesiology, Aleris-Hamlet Hospitals, Soeborg, Denmark, 2Department of Gynecology, Aleris-Hamlet Hospitals, Soeborg, Denmark


JoVE 50045

Date Published: June 25, 2013  |  doi:10.3791/50045  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Identification of Disease-related Spatial Covariance Patterns using Neuroimaging Data

1Center for Neurosciences, The Feinstein Institute for Medical Research


JoVE 50319

Date Published: June 26, 2013  |  doi:10.3791/50319  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Bioluminescent Orthotopic Model of Pancreatic Cancer Progression

1Monash Institute of Pharmaceutical Sciences, Monash University, 2Department of Visceral Surgery and Medicine, University of Bern, 3Cousins Center for Neuroimmunology, University of California Los Angeles


JoVE 50395

Date Published: June 28, 2013  |  doi:10.3791/50395  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Osmotic Drug Delivery to Ischemic Hindlimbs and Perfusion of Vasculature with Microfil for Micro-Computed Tomography Imaging

1The Texas Heart Institute at St. Luke's Episcopal Hospital, 2Department of Vascular Surgery, The Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University


JoVE 50364

Date Published: June 29, 2013  |  doi:10.3791/50364  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Fetal Echocardiography and Pulsed-wave Doppler Ultrasound in a Rabbit Model of Intrauterine Growth Restriction

1Division Woman and Child, Department Women, University Hospitals Leuven, 2The Ritchie Centre, Monash Institute of Medical Research, Department of Obstetrics and Gynaecology, Monash University, Victoria, Australia, 3Department of Cardiovascular Sciences, Katholieke Universiteit Leuven, 4Fetal and Perinatal Medicine Research Group, Institut d'Investigacions Biomediques August Pi i Sunyer (IDIBAPS), 5Maternal-Fetal Medicine Department, ICGON, Hospital Clínic, Universitat de Barcelona, Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER)


JoVE 50392

Date Published: June 29, 2013  |  doi:10.3791/50392  |  Section: JoVE Clinical and Translational Medicine

 JoVE Bioengineering

Photoacoustic Cystography

1Department of Biomedical Engineering, University at Buffalo, The State University of New York, 2Department of Creative IT Engineering, Pohang University of Science and Technology (POSTECH), 3School of Electrical Engineering and Computer Science, Kyungpook National University


JoVE 50340

Date Published: June 11, 2013  |  doi:10.3791/50340  |  Section: JoVE Bioengineering

 JoVE Bioengineering

High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots

1Application Development, Akonni Biosystems, Inc., 2Manufacturing, Akonni Biosystems, Inc., 3Engineering, Akonni Biosystems, Inc., 4Research & Development, Akonni Biosystems, Inc.


JoVE 50356

Date Published: June 11, 2013  |  doi:10.3791/50356  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Analysis of Targeted Viral Protein Nanoparticles Delivered to HER2+ Tumors

1Department of Biomedical Engineering, University of Southern California, 2Department of Biomedical Sciences, Cedars-Sinai Medical Center, 3Geffen School of Medicine, University of California, Los Angeles


JoVE 50396

Date Published: June 18, 2013  |  doi:10.3791/50396  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Determination of the Transport Rate of Xenobiotics and Nanomaterials Across the Placenta using the ex vivo Human Placental Perfusion Model

1Department of Obstetrics, Perinatal Pharmacology, University Hospital Zurich, 2Laboratory for Materials - Biology Interactions, EMPA Swiss Federal Laboratories for Materials Testing and Research, 3Graduate School for Cellular and Biomedical Sciences, University of Bern


JoVE 50401

Date Published: June 18, 2013  |  doi:10.3791/50401  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

1Department of Structural Biology, University of Pittsburgh School of Medicine, 2Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine


JoVE 50386

Date Published: June 24, 2013  |  doi:10.3791/50386  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy

1Department of Physics, Worcester Polytechnic Institute, 2Department of Chemical Engineering, Worcester Polytechnic Institute


JoVE 50497

Date Published: June 27, 2013  |  doi:10.3791/50497  |  Section: JoVE Bioengineering

 JoVE Applied Physics

Ultrahigh Density Array of Vertically Aligned Small-molecular Organic Nanowires on Arbitrary Substrates

1Electrical and Computer Engineering, University of Alberta


JoVE 50706

Date Published: June 18, 2013  |  doi:10.3791/50706  |  Section: JoVE Applied Physics

 JoVE Applied Physics

High-speed Particle Image Velocimetry Near Surfaces

1Department of Mechanical Engineering, University of Michigan


JoVE 50559

Date Published: June 24, 2013  |  doi:10.3791/50559  |  Section: JoVE Applied Physics

 JoVE Chemistry

Determination of the Gas-phase Acidities of Oligopeptides

1Department of Chemistry, University of the Pacific


JoVE 4348

Date Published: June 24, 2013  |  doi:10.3791/4348  |  Section: JoVE Chemistry

 JoVE Behavior

Functional Magnetic Resonance Imaging (fMRI) with Auditory Stimulation in Songbirds

1Bio-Imaging Lab, University of Antwerp


JoVE 4369

Date Published: June 3, 2013  |  doi:10.3791/4369  |  Section: JoVE Behavior

 JoVE Behavior

Best Current Practice for Obtaining High Quality EEG Data During Simultaneous fMRI

1Sir Peter Mansfield Magnetic Resonance Centre, School of Physics and Astronomy, University of Nottingham, 2Brain Products GmbH


JoVE 50283

Date Published: June 3, 2013  |  doi:10.3791/50283  |  Section: JoVE Behavior

 JoVE Behavior

Perceptual and Category Processing of the Uncanny Valley Hypothesis' Dimension of Human Likeness: Some Methodological Issues

1Department of Neuropsychology, University of Zurich


JoVE 4375

Date Published: June 3, 2013  |  doi:10.3791/4375  |  Section: JoVE Behavior

 JoVE Behavior

How to Detect Amygdala Activity with Magnetoencephalography using Source Imaging

1Department of Psychology, University of Wisconsin-Milwaukee, 2McConnell Brain Imaging Centre, Montreal Neurological Institute, McGill University, 3Department of Neurology, Medical College of Wisconsin


JoVE 50212

Date Published: June 3, 2013  |  doi:10.3791/50212  |  Section: JoVE Behavior

 JoVE Behavior

Stereotactic Injection of MicroRNA-expressing Lentiviruses to the Mouse Hippocampus CA1 Region and Assessment of the Behavioral Outcome

1Department of Biological Chemistry and The Edmond & Lily Safra Center for Brain Sciences, The Hebrew University of Jerusalem


JoVE 50170

Date Published: June 10, 2013  |  doi:10.3791/50170  |  Section: JoVE Behavior

 JoVE Behavior

Using Chronic Social Stress to Model Postpartum Depression in Lactating Rodents

1Biomedical Sciences, Tufts University Cummings School of Veterinary Medicine, 2School of Healthcare Sciences, Manchester Metropolitan University


JoVE 50324

Date Published: June 10, 2013  |  doi:10.3791/50324  |  Section: JoVE Behavior

 JoVE Behavior

Use of the Operant Orofacial Pain Assessment Device (OPAD) to Measure Changes in Nociceptive Behavior

1Department of Oral and Maxillofacial Surgery, University of Florida College of Dentistry, 2Department of Neuroscience, McKnight Brain Institute, University of Florida College of Medicine, 3Stoelting Co., 4Department of Orthodontics, University of Florida


JoVE 50336

Date Published: June 10, 2013  |  doi:10.3791/50336  |  Section: JoVE Behavior

 JoVE Behavior

Assessment of Sensorimotor Function in Mouse Models of Parkinson's Disease

1Department of Psychology, University of Cincinnati, 2Department of Neurology, University of Cincinnati


JoVE 50303

Date Published: June 17, 2013  |  doi:10.3791/50303  |  Section: JoVE Behavior

 JoVE Behavior

Simultaneous EEG Monitoring During Transcranial Direct Current Stimulation

1Programa de Pós-Graduação em Ciências Médica, Universidade Federal do Rio Grande do Sul, 2Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), 3Laboratory of Neuromodulation, Department of Physical Medicine & Rehabilitation, Spaulding Rehabilitation Hospital and Massachusetts General Hospital, Harvard Medical School, 4De Montfort University


JoVE 50426

Date Published: June 17, 2013  |  doi:10.3791/50426  |  Section: JoVE Behavior

 JoVE Behavior

Mouse Short- and Long-term Locomotor Activity Analyzed by Video Tracking Software

1Department of Animal Sciences, University of Illinois at Urbana-Champaign, 2Department of Pathology, Integrative Immunology and Behavior Program, University of Illinois at Urbana-Champaign


JoVE 50252

Date Published: June 20, 2013  |  doi:10.3791/50252  |  Section: JoVE Behavior

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